{"title":"Mir-let-7a在口腔鳞状细胞癌和口腔扁平苔藓中的差异表达:早期诊断生物标志物发展的见解","authors":"Seyedmovahed Seyedhoseini, Nooshin Mohtasham, Shadi Saghafi, Negin Alahkhani, Farnaz Mohajertehran, Fahimeh Afzaljavan, Mahammad Taghi Shakeri, Amirkian Bokharaei","doi":"10.61186/rbmb.13.4.456","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Early detection of oral squamous cell carcinoma (OSCC) is essential for improving treatment outcomes. Oral lichen planus (OLP) is recognized as a premalignant condition that may progress to OSCC. Recently, microRNAs, particularly miR-let-7a, have emerged as promising biomarkers for gene regulation and early disease diagnosis. This study aimed to evaluate the expression level of miR-let-7a in OSCC and OLP patients, and to compare it with healthy controls, to determine its potential as an early diagnostic marker.</p><p><strong>Methods: </strong>In this cross-sectional study, serum samples were collected from 36 OSCC patients, 38 OLP patients, and 38 healthy controls. Diagnosis of OSCC and OLP was confirmed via biopsy. Serum RNA was isolated, and after quality verification, cDNA was synthesized. Quantitative real-time PCR (qRT-PCR) was performed to assess miR-let-7a expression across the three groups. Statistical analysis was conducted using SPSS version 16.0.</p><p><strong>Results: </strong>Significant differences in miR-let-7a expression were observed among the groups. Mean expression levels of miR-let-7a were 1.55 ± 1.19 in OSCC, 2.97 ± 2.00 in OLP, and 7.02 ± 4.10 in the control group (p< 0.001). Lower miR-let-7a expression in OSCC was notably correlated with adverse clinicopathological features, including higher tumor grade (p < 0.001), advanced clinical stage (p= 0.011), larger tumor size (T2) (p< 0.0001), and lymph node involvement (p< 0.0001).</p><p><strong>Conclusions: </strong>The findings demonstrate that miR-let-7a expression is significantly reduced in OSCC and OLP patients compared to healthy controls, highlighting its potential as an early biomarker for detecting malignant transformation in oral lesions and understanding disease progression in OSCC and OLP.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 4","pages":"456-465"},"PeriodicalIF":1.2000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367217/pdf/","citationCount":"0","resultStr":"{\"title\":\"Mir-let-7a Differential Expression in Oral Squamous Cell Carcinoma and Oral Lichen Planus: Insights for Early Diagnostic Biomarker Development.\",\"authors\":\"Seyedmovahed Seyedhoseini, Nooshin Mohtasham, Shadi Saghafi, Negin Alahkhani, Farnaz Mohajertehran, Fahimeh Afzaljavan, Mahammad Taghi Shakeri, Amirkian Bokharaei\",\"doi\":\"10.61186/rbmb.13.4.456\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Early detection of oral squamous cell carcinoma (OSCC) is essential for improving treatment outcomes. Oral lichen planus (OLP) is recognized as a premalignant condition that may progress to OSCC. Recently, microRNAs, particularly miR-let-7a, have emerged as promising biomarkers for gene regulation and early disease diagnosis. This study aimed to evaluate the expression level of miR-let-7a in OSCC and OLP patients, and to compare it with healthy controls, to determine its potential as an early diagnostic marker.</p><p><strong>Methods: </strong>In this cross-sectional study, serum samples were collected from 36 OSCC patients, 38 OLP patients, and 38 healthy controls. Diagnosis of OSCC and OLP was confirmed via biopsy. Serum RNA was isolated, and after quality verification, cDNA was synthesized. Quantitative real-time PCR (qRT-PCR) was performed to assess miR-let-7a expression across the three groups. Statistical analysis was conducted using SPSS version 16.0.</p><p><strong>Results: </strong>Significant differences in miR-let-7a expression were observed among the groups. Mean expression levels of miR-let-7a were 1.55 ± 1.19 in OSCC, 2.97 ± 2.00 in OLP, and 7.02 ± 4.10 in the control group (p< 0.001). Lower miR-let-7a expression in OSCC was notably correlated with adverse clinicopathological features, including higher tumor grade (p < 0.001), advanced clinical stage (p= 0.011), larger tumor size (T2) (p< 0.0001), and lymph node involvement (p< 0.0001).</p><p><strong>Conclusions: </strong>The findings demonstrate that miR-let-7a expression is significantly reduced in OSCC and OLP patients compared to healthy controls, highlighting its potential as an early biomarker for detecting malignant transformation in oral lesions and understanding disease progression in OSCC and OLP.</p>\",\"PeriodicalId\":45319,\"journal\":{\"name\":\"Reports of Biochemistry and Molecular Biology\",\"volume\":\"13 4\",\"pages\":\"456-465\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367217/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reports of Biochemistry and Molecular Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.61186/rbmb.13.4.456\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reports of Biochemistry and Molecular Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.61186/rbmb.13.4.456","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Mir-let-7a Differential Expression in Oral Squamous Cell Carcinoma and Oral Lichen Planus: Insights for Early Diagnostic Biomarker Development.
Background: Early detection of oral squamous cell carcinoma (OSCC) is essential for improving treatment outcomes. Oral lichen planus (OLP) is recognized as a premalignant condition that may progress to OSCC. Recently, microRNAs, particularly miR-let-7a, have emerged as promising biomarkers for gene regulation and early disease diagnosis. This study aimed to evaluate the expression level of miR-let-7a in OSCC and OLP patients, and to compare it with healthy controls, to determine its potential as an early diagnostic marker.
Methods: In this cross-sectional study, serum samples were collected from 36 OSCC patients, 38 OLP patients, and 38 healthy controls. Diagnosis of OSCC and OLP was confirmed via biopsy. Serum RNA was isolated, and after quality verification, cDNA was synthesized. Quantitative real-time PCR (qRT-PCR) was performed to assess miR-let-7a expression across the three groups. Statistical analysis was conducted using SPSS version 16.0.
Results: Significant differences in miR-let-7a expression were observed among the groups. Mean expression levels of miR-let-7a were 1.55 ± 1.19 in OSCC, 2.97 ± 2.00 in OLP, and 7.02 ± 4.10 in the control group (p< 0.001). Lower miR-let-7a expression in OSCC was notably correlated with adverse clinicopathological features, including higher tumor grade (p < 0.001), advanced clinical stage (p= 0.011), larger tumor size (T2) (p< 0.0001), and lymph node involvement (p< 0.0001).
Conclusions: The findings demonstrate that miR-let-7a expression is significantly reduced in OSCC and OLP patients compared to healthy controls, highlighting its potential as an early biomarker for detecting malignant transformation in oral lesions and understanding disease progression in OSCC and OLP.
期刊介绍:
The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.