Mir-let-7a在口腔鳞状细胞癌和口腔扁平苔藓中的差异表达:早期诊断生物标志物发展的见解

IF 1.2 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Seyedmovahed Seyedhoseini, Nooshin Mohtasham, Shadi Saghafi, Negin Alahkhani, Farnaz Mohajertehran, Fahimeh Afzaljavan, Mahammad Taghi Shakeri, Amirkian Bokharaei
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引用次数: 0

摘要

背景:早期发现口腔鳞状细胞癌(OSCC)对于改善治疗效果至关重要。口腔扁平苔藓(OLP)被认为是一种可能发展为OSCC的癌前病变。最近,microrna,特别是miR-let-7a,已经成为基因调控和早期疾病诊断的有希望的生物标志物。本研究旨在评估miR-let-7a在OSCC和OLP患者中的表达水平,并将其与健康对照进行比较,以确定其作为早期诊断标志物的潜力。方法:在横断面研究中,收集36例OSCC患者、38例OLP患者和38例健康对照者的血清样本。通过活检确诊OSCC和OLP。分离血清RNA,经质量验证后合成cDNA。采用实时荧光定量PCR (qRT-PCR)评估miR-let-7a在三组中的表达。采用SPSS 16.0进行统计分析。结果:各组间miR-let-7a表达差异有统计学意义。miR-let-7a在OSCC组的平均表达水平为1.55±1.19,OLP组为2.97±2.00,对照组为7.02±4.10 (p< 0.001)。miR-let-7a在OSCC中的低表达与不良临床病理特征显著相关,包括肿瘤分级高(p< 0.001)、临床分期晚(p= 0.011)、肿瘤体积大(p< 0.0001)、淋巴结受累(p< 0.0001)。结论:研究结果表明,与健康对照组相比,miR-let-7a在OSCC和OLP患者中的表达显著降低,突出了其作为检测口腔病变恶性转化和了解OSCC和OLP疾病进展的早期生物标志物的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mir-let-7a Differential Expression in Oral Squamous Cell Carcinoma and Oral Lichen Planus: Insights for Early Diagnostic Biomarker Development.

Background: Early detection of oral squamous cell carcinoma (OSCC) is essential for improving treatment outcomes. Oral lichen planus (OLP) is recognized as a premalignant condition that may progress to OSCC. Recently, microRNAs, particularly miR-let-7a, have emerged as promising biomarkers for gene regulation and early disease diagnosis. This study aimed to evaluate the expression level of miR-let-7a in OSCC and OLP patients, and to compare it with healthy controls, to determine its potential as an early diagnostic marker.

Methods: In this cross-sectional study, serum samples were collected from 36 OSCC patients, 38 OLP patients, and 38 healthy controls. Diagnosis of OSCC and OLP was confirmed via biopsy. Serum RNA was isolated, and after quality verification, cDNA was synthesized. Quantitative real-time PCR (qRT-PCR) was performed to assess miR-let-7a expression across the three groups. Statistical analysis was conducted using SPSS version 16.0.

Results: Significant differences in miR-let-7a expression were observed among the groups. Mean expression levels of miR-let-7a were 1.55 ± 1.19 in OSCC, 2.97 ± 2.00 in OLP, and 7.02 ± 4.10 in the control group (p< 0.001). Lower miR-let-7a expression in OSCC was notably correlated with adverse clinicopathological features, including higher tumor grade (p < 0.001), advanced clinical stage (p= 0.011), larger tumor size (T2) (p< 0.0001), and lymph node involvement (p< 0.0001).

Conclusions: The findings demonstrate that miR-let-7a expression is significantly reduced in OSCC and OLP patients compared to healthy controls, highlighting its potential as an early biomarker for detecting malignant transformation in oral lesions and understanding disease progression in OSCC and OLP.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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