Comparative Analysis of Curcumin and Metformin Effects on the Kidney in a Model of Unilateral Nephrectomy and Ischemia-Reperfusion Injury.

IF 1.2 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Samaneh Karimi, Farhad Koohpeyma, Layasadat Khorsandi, Armin Panahi, Fatemeh Rezaei-Tazangi, Fereshtesadat Fakhredini
{"title":"Comparative Analysis of Curcumin and Metformin Effects on the Kidney in a Model of Unilateral Nephrectomy and Ischemia-Reperfusion Injury.","authors":"Samaneh Karimi, Farhad Koohpeyma, Layasadat Khorsandi, Armin Panahi, Fatemeh Rezaei-Tazangi, Fereshtesadat Fakhredini","doi":"10.61186/rbmb.13.4.579","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Unilateral nephrectomy and renal ischemia-reperfusion injury (I/R) are causes of acute kidney injury that can cause renal dysfunction, decreased glomerular filtration rate, impaired homeostasis, and increased mortality. This study aimed to evaluate the combined effects of curcumin (Cur) and metformin (Met), both protective antioxidants, on renal tissue structure and Neutrophil gelatinase-associated lipocalin (Ngal) and Kidney injury molecule 1 (Kim-1) genes expression levels in rats undergoing unilateral nephrectomy and ischemia/reperfusion.</p><p><strong>Methods: </strong>In this experimental study, 40 male rats were randomly divided into five groups. The animals underwent unilateral nephrectomy and ischemia/reperfusion and were then treated with curcumin and metformin or both for 14 days. Subsequently, histopathological and morphometric analyses were performed, and Ngal and Kim-1 gene expression levels were evaluated using real-time PCR.</p><p><strong>Results: </strong>Histopathological damage, expression levels of blood urea nitrogen (BUN), creatinine (Cr), blood biochemical parameters and oxidative stress and expression levels of Ngal and Kim-1 genes were significantly reduced in the patient group that received metformin and curcumin simultaneously. However, total antioxidant capacity (TAC) was increased in the patient group that received curcumin + metformin. Morphometric parameters also improved in this group.</p><p><strong>Conclusions: </strong>The results showed that the combination therapy with curcumin and metformin effectively protected the kidneys against unilateral nephrectomy and I/R injury.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"13 4","pages":"579-589"},"PeriodicalIF":1.2000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367211/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reports of Biochemistry and Molecular Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.61186/rbmb.13.4.579","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Unilateral nephrectomy and renal ischemia-reperfusion injury (I/R) are causes of acute kidney injury that can cause renal dysfunction, decreased glomerular filtration rate, impaired homeostasis, and increased mortality. This study aimed to evaluate the combined effects of curcumin (Cur) and metformin (Met), both protective antioxidants, on renal tissue structure and Neutrophil gelatinase-associated lipocalin (Ngal) and Kidney injury molecule 1 (Kim-1) genes expression levels in rats undergoing unilateral nephrectomy and ischemia/reperfusion.

Methods: In this experimental study, 40 male rats were randomly divided into five groups. The animals underwent unilateral nephrectomy and ischemia/reperfusion and were then treated with curcumin and metformin or both for 14 days. Subsequently, histopathological and morphometric analyses were performed, and Ngal and Kim-1 gene expression levels were evaluated using real-time PCR.

Results: Histopathological damage, expression levels of blood urea nitrogen (BUN), creatinine (Cr), blood biochemical parameters and oxidative stress and expression levels of Ngal and Kim-1 genes were significantly reduced in the patient group that received metformin and curcumin simultaneously. However, total antioxidant capacity (TAC) was increased in the patient group that received curcumin + metformin. Morphometric parameters also improved in this group.

Conclusions: The results showed that the combination therapy with curcumin and metformin effectively protected the kidneys against unilateral nephrectomy and I/R injury.

姜黄素与二甲双胍对单侧肾切除术及缺血再灌注损伤模型肾脏影响的比较分析。
背景:单侧肾切除术和肾缺血再灌注损伤(I/R)是急性肾损伤的原因,可导致肾功能障碍、肾小球滤过率下降、体内平衡受损和死亡率增加。本研究旨在探讨姜黄素(curcumin, Cur)和二甲双胍(metformin, Met)对单侧肾切除术及缺血再灌注大鼠肾组织结构及中性粒细胞明胶酶相关脂钙蛋白(Neutrophil gelatinase-associated lipocalin, Ngal)和肾损伤分子1 (Kidney injury molecule 1, Kim-1)基因表达的影响。方法:选取40只雄性大鼠,随机分为5组。经单侧肾切除和缺血再灌注治疗后,给予姜黄素和二甲双胍或两者联合治疗14天。随后,进行组织病理学和形态计量学分析,并使用实时PCR评估Ngal和Kim-1基因表达水平。结果:二甲双胍与姜黄素联合用药组组织病理损伤、血尿素氮(BUN)、肌酐(Cr)、血液生化指标、氧化应激表达水平及Ngal、Kim-1基因表达水平均显著降低。然而,接受姜黄素+二甲双胍治疗的患者组总抗氧化能力(TAC)增加。形态学参数也得到改善。结论:姜黄素联合二甲双胍治疗对单侧肾切除术及肾I/R损伤有保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信