脂质体维生素C对肾细胞中SARS-CoV-2靶病毒进入基因的保护作用

IF 1.2 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Abtin Behmardi, Leila Zolghadr, Farzad Rajaei, Saeideh Gholamzadeh Khoei, Nematollah Gheibi
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引用次数: 0

摘要

背景:肾脏是SARS-CoV-2感染的潜在靶点。抗坏血酸(维生素C)已被证明在减轻SARS-CoV-2症状方面发挥重要作用。最近,脂质体给药平台在提高包括传染病在内的各种治疗方法的有效性方面显示出有希望的结果。在本研究中,我们设计了一种含维生素C的脂质体给药系统,以评估其对COVID-19的抗病毒效果,重点关注其对病毒进入Vero细胞基因表达的影响。方法:将维生素C装入由氢化大豆磷脂酰胆碱、胆固醇和1,2-二硬脂酰-sn-甘油-3磷酸乙醇胺- n-[甲氧基(聚乙二醇)-2000]组成的脂质体中,并对其理化性质进行评价。接下来,使用MTT法评估游离和脂质体维生素C对Vero细胞系存活的细胞毒性。此外,我们还利用RTq-PCR检测了SARS-CoV-2进入肾细胞的关键介质——病毒进入基因血管紧张素转换酶2 (ACE2)和跨膜蛋白酶丝氨酸2 (TMPRSS2)的表达。结果:脂质体成功负载维生素C,包封率为88.03%。通过扫描电镜观察,维生素C脂质体制剂的表面形态非常明亮。游离和脂质体形式的维生素C在较高浓度下均表现出细胞毒性作用。此外,两种形式都下调了病毒进入基因的表达,尽管脂质体形式比自由形式表现出更好的抑制性能。结论:该研究表明,脂质体维生素C可以靶向肾细胞中的病毒进入基因,保护肾细胞免受病毒损伤和炎症,从而安全有效地治疗COVID-19。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective Effects of Liposomal Vitamin C on SARS-CoV-2 Target Viral Entry Genes in Renal Cells.

Background: The kidneys are a potential target for SARS-CoV-2 infection. Ascorbic acid (vitamin C) has been shown to play an important role in reducing the symptoms of SARS-CoV-2. Recently liposomal drug delivery platforms have demonstrated promising results in enhancing the effectiveness of various therapeutics including infectious diseases. In this study, we designed a liposomal delivery system containing vitamin C to evaluate its antiviral efficacy in COVID-19, focusing on its effects on viral entry gene expression in Vero cells.

Methods: Vitamin C was loaded into a liposome made up of hydrogenated soybean phosphatidylcholine, cholesterol, and 1,2-distearoyl-sn-glycero-3 phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000], and their physicochemical properties were assessed. Next, the cytotoxicity of free and liposomal vitamin C on the survival of the Vero cell line was evaluated using the MTT assay. In addition, the expression of viral entry genes, angiotensin-converting enzyme 2 (ACE2) and transmembrane protease serine 2 (TMPRSS2), key mediators of SARS-CoV-2 entry into kidney cells, was investigated using RTq-PCR.

Results: Liposomes were successfully loaded with vitamin C, achieving an encapsulation efficiency of 88.03%. The liposomal vitamin C formulation exhibited a brilliant surface morphology as observed by SEM. Both free and liposomal forms of vitamin C showed cytotoxic effects at higher concentrations. Moreover, both forms downregulated the expression of viral entry genes, although the liposomal form showed superior inhibitory performance compared to the free form.

Conclusions: The study suggests liposomal vitamin C as a safe, effective treatment for COVID-19 by targeting viral entry genes in kidney cells, protecting them from viral damage and inflammation.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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