Sensing and Bio-Sensing Research最新文献

{"title":"“All-on-a-Tube” POCT of Salmonella in large-volume sample","authors":"Lei Wang,&nbsp;Nana Jin,&nbsp;Meixuan Li,&nbsp;Jianhan Lin","doi":"10.1016/j.sbsr.2024.100712","DOIUrl":"10.1016/j.sbsr.2024.100712","url":null,"abstract":"<div><div>Point-of-care testing (POCT) of pathogenic bacteria at low concentrations is vital to early warning of bacterial contaminations. A disposable centrifuge tube was reconstructed in this study for sensitive <em>Salmonella</em> detection in large-volume samples, where the entire bacterial detection progress from separation to detection, was performed within the tube. The bottom of this centrifuge tube was assembled with a flexible stirrer containing a pair of circular magnets, which was rapidly rotated using a DC motor to produce the vortex for simultaneous mixing and capture of target bacteria. Besides, immune manganese dioxide nanoflowers were synthesized and used to label target bacteria, followed by mimicking catalyze colorless TMB substrate into blue TMBox product. The product image was captured and analyzed by a smartphone App to quantitatively determine the target bacteria. This POCT centrifuge tube effectively achieved a separation efficiency of approximately 80 % for target bacteria from a 10 mL sample, enabling the detection of target bacteria within the range of 1.3 × 10¹ to 1.3 × 10⁴ CFU/mL in 1 h, with a low detection limit of 13 CFU/mL. More importantly, this reconstructed centrifuge tube demonstrated its potential as a laboratory consumable for bacterial detection in routine screening owing to the features of low cost, easy operation and high integration.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100712"},"PeriodicalIF":5.4,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of flexible polyimide-based serpentine EMG sensor for AI-enabled fatigue detection in construction","authors":"Yogesh Gautam,&nbsp;Houtan Jebelli","doi":"10.1016/j.sbsr.2024.100713","DOIUrl":"10.1016/j.sbsr.2024.100713","url":null,"abstract":"<div><div>Physical fatigue and musculoskeletal disorders are critical health issues for construction workers, stemming from repetitive motions, heavy lifting, and awkward postures. These factors compromise worker well-being, productivity, and safety while increasing the risk of accidents and long-term health problems. Recent advancements in wearable health monitoring technology offer potential solutions, but current sensors encounter significant challenges in the dynamic construction environment. These include inadequate skin contact, increased contact impedance, and vulnerability to motion artifacts all of which degrade signal quality and reduce the accuracy of fatigue detection. This paper develops a fractal-based, flexible sensor for enhanced adaptability and accurate fatigue estimation. Finite element analysis compared five space-filling designs, with the serpentine curve exhibiting the highest contact area and lowest strain, making it the preferred choice for fabrication. Evaluations demonstrated significant improvements in signal-to-noise ratio (SNR) and motion artifact reduction, with the newly developed sensor achieving a 37 % to 59 % SNR improvement over commercial electrodes across different muscle groups. The developed flexible sensor was integrated with a fatigue-detecting framework based on a vision transformer model which provided an average accuracy of 87 % for fatigue detection. The developed sensor significantly enhances EMG signal quality and reliability, promising improved health monitoring and safety for construction workers.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100713"},"PeriodicalIF":5.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular displacement approach for the electrochemical detection of protein-bound propofol","authors":"David C. Ferrier,&nbsp;Janice Kiely,&nbsp;Richard Luxton","doi":"10.1016/j.sbsr.2024.100710","DOIUrl":"10.1016/j.sbsr.2024.100710","url":null,"abstract":"<div><div>Propofol is one of the principal drugs used for the sedation of patients undergoing mechanical ventilation in intensive care units. The correct dosage of such sedative drugs is highly important, but current methods of determining infusion rates are limited and there is a lack of suitable methods for directly determining patient blood propofol concentrations. A significant challenge for the development of propofol sensors is that propofol demonstrates very high protein binding, leading to a low free fraction in blood. Here we present a method for improving the efficacy of an electrochemical propofol sensor by increasing the free fraction via a molecular displacement approach. When used in conjunction with a carbon nanotube/graphene oxide/iron oxide nanoparticle functionalised screen-printed electrode, it was found that this approach dramatically improved the sensor's sensitivity towards propofol. Ibuprofen was found to be the most effective displacement agent, with an optimal concentration of 30 mM. The resultant sensitivity was 2.82 nA/μg/ml/mm² with a coefficient of variation of 0.07, and the limit of detection was 0.2 μg/ml. This approach demonstrates high specificity towards drugs commonly administered to intensive care patients.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100710"},"PeriodicalIF":5.4,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosensor for integrin inhibition of mammalian cell adhesion and migration using micropatterned cell culture substrate and retroreflective optical signaling","authors":"Kyung Won Lee ,&nbsp;Eun Kyeong Yang ,&nbsp;Duc Long Nguyen ,&nbsp;Soo A Park ,&nbsp;Moon Suk Kim ,&nbsp;Jae-Ho Kim ,&nbsp;Min Young Lee ,&nbsp;Hyun C. Yoon","doi":"10.1016/j.sbsr.2024.100714","DOIUrl":"10.1016/j.sbsr.2024.100714","url":null,"abstract":"<div><div>Integrins are a family of transmembrane receptors that play a crucial role in cell adhesion and migration. Integrins can uniquely transduce biochemical signals bidirectionally across the membrane and physically link the cell-cell and cell-extracellular matrix (ECM) with ligand bonds. The arginyl-glycyl-aspartic acid (RGD) peptide motif is present in the ECM as a minimal recognition sequence for integrins. To leverage this property in cell-based therapy, RGD variants, such as cyclic-type RGDfK (c(RGDfK)), which share a similar structure with RGD but exhibit a higher affinity for integrins, have been developed. However, because most evaluation methods for newly developed RGD variants focus on affinity strength, tools for cellular effects are required. In this study, we developed a new platform that integrates micropatterned three-dimensional cell culture substrates with a non-spectroscopic optical analysis system to quantitatively analyze the effects of RGD variants on cell adhesion and migration. The specially micropatterned substrate provides a cell adhesive and migration area to provide a restricted analysis area. Owing to the characteristics of retroreflective Janus particles (RJPs), a non-spectroscopic optical analysis system provides long-term stable optical verification properties and a simple optical setup. These techniques were integrated to quantitatively determine the integrin inhibitory effect of various concentrations of RGD variant. To demonstrate the efficacy of the developed cellular level RGD variant testing platform, the model cell line L929 fibroblast and model RGD variant c(RGDfK) were analyzed ranging from 0 to 10 μM. The results showed that the developed system could effectively and quantitatively analyze the effects of RGD variants on cells across various concentrations.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100714"},"PeriodicalIF":5.4,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in electrochemiluminescence-based sensors for ultra-sensitive pesticide residue detection","authors":"Irkham ,&nbsp;Chico Permana Putra ,&nbsp;Clianta Yudin Kharismasari ,&nbsp;Salma Nur Zakiyyah ,&nbsp;Isnaini Rahmawati ,&nbsp;Ivandini Tribidasari Anggraningrum ,&nbsp;Wulan Tri Wahyuni ,&nbsp;Giovanni Valenti ,&nbsp;Francesco Paolucci ,&nbsp;Yeni Wahyuni Hartati","doi":"10.1016/j.sbsr.2024.100708","DOIUrl":"10.1016/j.sbsr.2024.100708","url":null,"abstract":"<div><div>Pesticides are chemical substances utilized to prevent, control, and eradicate pests or organisms detrimental to crops, playing a pivotal role in minimizing agricultural losses and enhancing yields. However, improper pesticide use can lead to residue accumulation in the surrounding environment, including agricultural products, soil, and water. Detecting pesticide residues in the environment has become imperative. While various techniques such as chromatography, spectrophotometry, optical sensors, and electrochemical biosensors have been reported for pesticide residue detection, they face limitations such as large instrument size and low sensitivity. Currently, electrochemiluminescence (ECL) methods have been developed for pesticide detection. ECL methods can be combined with biosensor techniques or other molecule recognition element to enhance selectivity and specificity. ECL combines controllable electrochemical reaction properties with high photokinetic sensitivity. It can detect analytes with low detection limits (down to pM levels), ensuring rapid measurements and requiring minimal sample volumes for analysis. This literature review discusses the progress in research on detecting pesticide residues using ECL methods. The utilization of ECL, with its unique capabilities, signifies a promising avenue for addressing the shortcomings of traditional detection methods and advancing the field of pesticide residue analysis.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100708"},"PeriodicalIF":5.4,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determining factor of enzyme conjugates, bridge heterology and analytical variables of immunogens in prednisolone ELISA","authors":"Dinesh Kumar ,&nbsp;Harinder Singh Oberoi ,&nbsp;Subash Chandra Sonkar ,&nbsp;Bidhan Chandra Koner ,&nbsp;T.G. Shrivastav","doi":"10.1016/j.sbsr.2024.100700","DOIUrl":"10.1016/j.sbsr.2024.100700","url":null,"abstract":"<div><div>In this study, adipic acid dihydrazide (ADH), carbohydrazide (CH), ethylenediamine (EDA), and urea (U) were used as spacer molecules, covalently linking prednisolone (PSL) to carrier proteins for immunogen preparation, and PSL to enzymes for enzyme conjugate preparation using N-hydroxysuccinimide (NHS)-mediated carbodiimide reactions. The resulting immunogens were used to generate antiserum in New Zealand white rabbits. Antibodies produced against immunogens with various spacers were tested for immunoreactivity with enzyme conjugates, both with and without spacers, in a total of twenty different combinations. All combinations demonstrated binding and were subsequently evaluated through displacement studies. Sensitivity and specificity tests revealed that the combination of PSL-21-HS-U-BSA-antibody with PSL-21-HS-HRP enzyme conjugate exhibited the best sensitivity (0.032 ng/mL) and limited cross-reactivity with other steroids. This combination was further examined for analytical parameters, showing recovery rates of 93.87–101.19 % for PSL from spiked human serum samples, with intra- and inter-assay CVs of &lt;8.88 %. The serum PSL values obtained by this method showed strong correlation with a commercially available ELISA kit (r² = 0.97, <em>n</em> = 78).</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100700"},"PeriodicalIF":5.4,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards yeast fermentation monitoring: Enhanced sensing performance with nanostructured platinum integrated microsensors array","authors":"Nurul Izni Rusli ,&nbsp;Ruben Van den Eeckhoudt ,&nbsp;Catarina Fernandes ,&nbsp;Filippo Franceschini ,&nbsp;Dimitrios Konstantinidis ,&nbsp;Kevin J. Verstrepen ,&nbsp;Frederik Ceyssens ,&nbsp;Michael Kraft ,&nbsp;Irene Taurino","doi":"10.1016/j.sbsr.2024.100709","DOIUrl":"10.1016/j.sbsr.2024.100709","url":null,"abstract":"<div><div>Effective and continuous monitoring of bioprocesses requires the parallel screening of multiple key parameters to enhance the processes and ultimately improve the quality of the end products. In this work, the development and characterization of only few square millimeters microfabricated multi-sensor array chip for analysis of yeast fermentation is described. We originally integrated platinum nanostructures (nano-Pt) on the microelectrodes by a simple, CMOS compatible, and scalable electrodeposition procedure. This step was proven to be pivotal to obtain highly sensitive and selective microsensors with minimal cross-talk and measurement variability. Nano-Pt enables reliable sensing at lower applied potentials, offering a promising solution to mitigate electrical cross-talk in closely integrated sensor configurations. The multi-sensor features potentiometric parallel-plate nanostructured electrodes for measuring pH, interdigitated nano-Pt electrodes for indirectly measuring microbial growth and activity by measuring the electrolyte conductivity, and microelectrodes based on nano-Pt for measuring dissolved oxygen (DO) and glucose <em>via</em> amperometry. Importantly, all-solid-state on-chip reference electrodes for potentiometric and amperometric sensors of this chip have been developed and characterized to enable standalone measurements and achieve true miniaturization, avoiding the need for external conventional reference electrodes. The chip includes a meander thin-film resistance temperature detector for temperature monitoring as well. Our platform represents the first step towards viable <em>in-situ</em> monitoring of lab-scale yeast fermentation and to control the homogeneity of process parameters in large scale bioreactors.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100709"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanopyramid copper structures on screen-printed carbon electrode for high-performance non-enzymatic glucose sensing: A cost-effective and scalable approach","authors":"Sahar Bakhshi ,&nbsp;Mahsa Rahmanipour ,&nbsp;Amir R. Amirsoleimani ,&nbsp;Mostafa Rezazadeh ,&nbsp;Hossein Siampour ,&nbsp;Ahmad Moshaii","doi":"10.1016/j.sbsr.2024.100706","DOIUrl":"10.1016/j.sbsr.2024.100706","url":null,"abstract":"<div><div>We report on the fabrication of a robust non-enzymatic glucose sensor featuring a sensing electrode composed of pyramid-shaped copper/copper oxide (Cu/Cu₂O) nanostructures formed through a simple electrodeposition process on a screen-printed carbon electrode (SPCE). The fabrication of Cu/Cu₂O nanostructures on the SPCE enhances the charge transfer and electrocatalytic performance of the sensor, proving advantageous for glucose sensing. Notably, this morphology contributes to electrochemical glucose determination over a wide linear range of 0.01 to 6 mM, with a sensitivity of 214.04 μA/(mM·cm²) and a low detection limit of 0.03 μM. The proposed simple approach ensures high reproducibility, stable attachment to the printed layer, and cost-effectiveness, making it well-suited for scalable production of non-enzymatic glucose sensors.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100706"},"PeriodicalIF":5.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold coated surface plasmon resonance based biosensor: An hexagonal photonic crystal Fiber platform","authors":"G.K.M. Hasanuzzaman,&nbsp;Tubassir Muhammad Sakib,&nbsp;Alok Kumar Paul","doi":"10.1016/j.sbsr.2023.100582","DOIUrl":"https://doi.org/10.1016/j.sbsr.2023.100582","url":null,"abstract":"<div><p>We numerically analyze a dual-core gold coated photonic crystal fiber (PCF) biosensor based on surface plasmon resonance that can be used for remote sensing applications in place of the traditional prism-based biosensor. Chemically stable active plasmonic material gold (Au) are positioned outside the fiber structure as a sensing layer to facilitate the construction of the sensor. From finite element method (FEM) based simulation, it is shown that the proposed sensor has the maximum wavelength sensitivity of 5000 nm/RIU. In addition, the amplitude sensitivity of the sensor is 267.66 RIU⁻¹ with the resolution of 2.00 × 10⁻⁵ RIU⁻¹ having sensing range of analyte refractive index 1.30 to 1.40. The suggested two-ring dual-core PCF sensor can be used to detect biological analytes, organic compounds, biomolecules, and other unidentified analytes due to its high sensitivity, enhanced sensing resolution, and suitable linearity properties. The proposed sensor has high sensitivity and wide operating range; from visible to mid-infrared which enables the detection of various biomolecules with different absorption or fluorescence spectra. Overall, the unique combination of structural design and optical properties make hexagonal PCF biosensor a promising and innovative platform for advanced and high-performance biosensing applications.</p></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"42 ","pages":"Article 100582"},"PeriodicalIF":5.3,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3211960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrocatalytic behaviour of self-assembled Cu-chitosan/f-MWCNT on glassy carbon electrode for detection of erythromycin in various samples","authors":"Prashu Jain,&nbsp;Sony Jagtap,&nbsp;Megha Chauhan,&nbsp;Ramani V. Motghare","doi":"10.1016/j.sbsr.2023.100568","DOIUrl":"https://doi.org/10.1016/j.sbsr.2023.100568","url":null,"abstract":"<div><p>Although antibiotics are class of compounds that are administered to fight bacterial infections in human and animals, its abuse is ensuing its occurrence in natural resources producing drug resistant strains, which in turn affect human health. Therefore, it is important to develop rapid and effective detection techniques for such antibiotics which are contaminating various natural resources. In this study, a novel electrochemical sensor was developed for determination of one of the most used macrolide antibiotic “Erythromycin” based on self- assembled cu-chitosan/f-MWCNT modified glassy carbon electrode. The negatively charged f-MWCNT holds the positively charged chitosan and Cu²⁺ ions, leading to formation of Cu-chitosan/f-MWCNT/GCE. FE-SEM, FT-IR, EDS as well as electrochemical methods such as CV, DPV and EIS were used to characterize formation of Cu-chitosan/f-MWCNT/GCE at every step of fabrication. From thermo-gravimetric analysis the composite was found to be stable up to 120 °C and further heating results in breakdown of the skeletal structure between 300 °C to 500 °C. Results from Cyclic voltammetry and electrochemical impedance spectroscopy indicated that after modification, the value of charge transfer resistance (Rct) decreased, and the electron transfer kinetics increased, significantly. In comparison to bare GCE, the Cu-chitosan/f-MWCNT/GCE displayed excellent electrocatalytic activity for the oxidation of erythromycin, as indicated by an increased oxidation peak current. The differential pulse peak current was linear for erythromycin concentration from 0.5 × 10⁻⁶ to 10 × 10⁻⁶ and 10 × 10⁻⁶ to 150 × 10⁻⁶M, with LOD 0.2 × 10⁻⁶M. The sensor demonstrated high selectivity, excellent stability, and impressive repeatability, during erythromycin determination. Thus, the proposed sensor demonstrates promising analytical applicability towards erythromycin detection in various samples.</p></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"41 ","pages":"Article 100568"},"PeriodicalIF":5.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1747627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}