Sensing and Bio-Sensing Research最新文献_第10页

CRISPR-Cas12a based detection of EGFR gene mutation in cell free DNA for early diagnosis of Non-Small Cell Lung Cancer (NSCLC) 基于CRISPR-Cas12a的游离细胞DNA EGFR基因突变检测在非小细胞肺癌（NSCLC）早期诊断中的应用

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100735

Syeda Najidah Shahni , Sarah Albogami , Bijay Pattnaik , Iqbal Azmi , Syed Mansoor Ali , Kapil Dev , Anant Mohan , Jawed Iqbal , Amit Sharma , Tanveer Ahmad

{"title":"CRISPR-Cas12a based detection of EGFR gene mutation in cell free DNA for early diagnosis of Non-Small Cell Lung Cancer (NSCLC)","authors":"Syeda Najidah Shahni , Sarah Albogami , Bijay Pattnaik , Iqbal Azmi , Syed Mansoor Ali , Kapil Dev , Anant Mohan , Jawed Iqbal , Amit Sharma , Tanveer Ahmad","doi":"10.1016/j.sbsr.2025.100735","DOIUrl":"10.1016/j.sbsr.2025.100735","url":null,"abstract":"<div><div>Detecting EGFR mutations in circulating cell-free DNA (cfDNA) is essential for personalizing treatment and real-time monitoring of non-small cell lung cancer (NSCLC), especially when tissue biopsies are not an option. Conventional methods for identifying these mutations in cfDNA are limited and often require extensive laboratory set-up. The CRISPR-Cas system, notably the Cas12a variant used in the DETECTR method, presents a groundbreaking approach for highly sensitive and specific mutation detection in biological fluids, significantly advancing diagnostic capabilities. In this study, we present a novel, rapid, and precise method combining Recombinase Polymerase Amplification (RPA) for DNA amplification with a CRISPR-Cas12a fluorescence assay to detect EGFR mutations. This technique achieves attomole-level sensitivity without cross-reactivity, functions near 37°C, and delivers results within an hour, making it highly suitable for clinical applications in precision oncology. To further enhance its utility for point-of-care diagnostics, we integrated Cas12a with lateral flow-based detection, achieving single-copy detection sensitivity. In a clinical trial involving 57 NSCLC patient samples, this method successfully identified EGFR mutations, with results consistent with those obtained via PCR. The CRISPR-Cas12a based lateral flow assay demonstrated exceptional sensitivity and specificity, even detecting dual mutations simultaneously on a single test strip. These results position the CRISPR-Cas12a detection system as a straightforward, highly sensitive alternative to PCR, with significant potential for point-of-care applications in detecting cfDNA mutations.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100735"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Indirect determination of free chlorine in seawater by cyclic voltammetry using graphite–epoxy composite electrode: Hydrogen adsorption capacity of graphite–epoxy composite is one–third of that of platinum 石墨-环氧复合电极循环伏安法间接测定海水中游离氯：石墨-环氧复合材料的氢吸附容量为铂的三分之一

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2024-12-01 DOI: 10.1016/j.sbsr.2024.100711

Kye Hak Ko

{"title":"Indirect determination of free chlorine in seawater by cyclic voltammetry using graphite–epoxy composite electrode: Hydrogen adsorption capacity of graphite–epoxy composite is one–third of that of platinum","authors":"Kye Hak Ko","doi":"10.1016/j.sbsr.2024.100711","DOIUrl":"10.1016/j.sbsr.2024.100711","url":null,"abstract":"<div><div>A new possibility of indirect determination of free chlorine using a graphite–epoxy composite(GEC) electrode instead of Pt disk electrode was suggested by interpreting the relationship between the peak current of the oxidation peak for hydrogen generated through water electrolysis in CV and the amount of the free chlorine. The linear response range of concentration was 0.06–0.2 mg∙L<sup>−1</sup> with correlation coefficient of 0.9951 (<em>n</em> = 5) and the sensitivity of 1225 μA cm<sup>−2</sup> mg<sup>−1</sup> L. The limit of detection (LOD) calculated from the 3<em>σ</em> IUPAC criteria was 1.2 × 10<sup>−2</sup> mg L<sup>−1</sup>. The relative standard deviation (<em>RSD</em>) to 0.06 mg L<sup>−1</sup> was 4.65 %(<em>n</em> = 10). The results show that the amount of free chlorine in the disinfected seawater can be indirectly determined by using a GEC electrode without influence of interferences unlike a Pt disk electrode. On the other hand, in this paper, a new method is proposed to evaluate the relative hydrogen adsorption capacity by the sensitivity of GEC electrode compared with that of Pt disk electrode. During the investigation of the hydrogen adsorption on the surface of the working electrode, we obtained the result that the hydrogen adsorption capacity of GEC is one-third of that of platinum.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100711"},"PeriodicalIF":5.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication and characterization of inkjet-printed interdigitated electrodes for non-faradaic electrochemical detection of uromodulin in urine 非法拉第电化学检测尿调素的喷墨印刷交叉电极的制备与表征

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2024-12-01 DOI: 10.1016/j.sbsr.2024.100715

Kunj Vora , Norbert Kordas , Karsten Seidl

{"title":"Fabrication and characterization of inkjet-printed interdigitated electrodes for non-faradaic electrochemical detection of uromodulin in urine","authors":"Kunj Vora , Norbert Kordas , Karsten Seidl","doi":"10.1016/j.sbsr.2024.100715","DOIUrl":"10.1016/j.sbsr.2024.100715","url":null,"abstract":"<div><div>This work describes the simulation, design, and fabrication of an inkjet-printed impedance biosensor for uromodulin which is a urine biomarker for kidney disease. The interdigitated electrodes (IDE) were fabricated through inkjet printing of silver ink on polyethylene terephthalate (PET) substrate and subsequently passivated with SU-8 for non-faradaic measurements. We functionalized the passivated IDE with biotin-streptavidin complex and uromodulin antibody to detect uromodulin (6.75 μg/ml-100 μg/ml). The measured impedance due to uromodulin immobilized from artificial urine samples showed a 27.4 % change in impedance magnitude per μg/ml of uromodulin at 150 Hz (log scale). The limit of detection achieved is 25 ng/ml. The sensor demonstrated specificity to uromodulin when measured against albumin, which is another protein biomarker present in the urine. These results highlight the possibility of implementing non-faradaic impedance measurements using a passivated IDE as the sensor element. It also promotes the sensor fabrication method of inkjet printing. The sensor concept shows the implementation potential of IDE as a single-use-sensor for point-of-care applications.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100715"},"PeriodicalIF":5.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overcoming challenges in plasmonic biosensors deployment for clinical and biomedical applications: A systematic review and meta-analysis 克服在临床和生物医学应用中部署等离子体生物传感器所面临的挑战：系统回顾与荟萃分析

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2024-11-20 DOI: 10.1016/j.sbsr.2024.100717

Patricia Ramirez-Priego , Elba Mauriz , Juliana Fátima Giarola , Laura M. Lechuga

{"title":"Overcoming challenges in plasmonic biosensors deployment for clinical and biomedical applications: A systematic review and meta-analysis","authors":"Patricia Ramirez-Priego , Elba Mauriz , Juliana Fátima Giarola , Laura M. Lechuga","doi":"10.1016/j.sbsr.2024.100717","DOIUrl":"10.1016/j.sbsr.2024.100717","url":null,"abstract":"<div><div>Over recent decades, plasmonic biosensors have positioned themselves as one of the most powerful analytical tools for evaluating biomolecular interactions. This impactful analytical technology has demonstrated its value in diverse fields such as clinical diagnostics, biotechnology, pharmaceutical evaluation, disease prevention, among others. Moreover, technological advances have led to the development of miniaturized plasmonic biosensing platforms, which are small and portable for point-of-care (POC) applications. This review presents an overview of recently implemented POC-plasmonic biosensors and their use for clinical and biomedical analysis. A systematic and deep literature search in PubMed, Scopus, and Web of Science databases was performed in publications between 1 January 2018 and 30 November 2023. We excluded many publications due to the absence of real application in complex matrices or insufficient analytical information. The quality of each study was evaluated methodologically by QUADAS-2, and a meta-analysis was performed using the Cochrane RevMan software. From 1177 full-text assessments, 27 research articles were selected. The pooled sensitivity was 4.36 (95%, CI 0.19–5.97, I<sup>2</sup> = 83%). The subgroup analysis according to the plasmonic sensors type revealed the best diagnostic odds ratio with the lowest heterogeneity: 3.81 (95% CI 0.39–39.08; I<sup>2</sup> = 33%, <em>p</em> = 0.22). These findings indicate that despite plasmonic biosensors taking advantage of their miniaturization and showing promising results in portable POC devices, there are still significant obstacles to using them as routine diagnostic tools. Estimating test accuracy may help to reduce the gap between controlled laboratory conditions and real-world clinical decision-making environments.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100717"},"PeriodicalIF":5.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Embedded optofluidic biosensing platform for enzyme-linked immunosorbent assay 用于酶联免疫吸附测定的嵌入式光流体生物传感平台

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2024-11-19 DOI: 10.1016/j.sbsr.2024.100716

Jie Zhang, Yuyao Li, Yuan Fang, Junzhe Wang, Erhan Zhuo, Jiekai Zhuo, Xia Ouyang, Zidan Gong

{"title":"Embedded optofluidic biosensing platform for enzyme-linked immunosorbent assay","authors":"Jie Zhang, Yuyao Li, Yuan Fang, Junzhe Wang, Erhan Zhuo, Jiekai Zhuo, Xia Ouyang, Zidan Gong","doi":"10.1016/j.sbsr.2024.100716","DOIUrl":"10.1016/j.sbsr.2024.100716","url":null,"abstract":"<div><div>With the growing demand for applications in disease diagnostics, bioanalysis, and health monitoring, developing efficient biosensing systems for fast detections and trace analysis of biomarkers is of great significance. In this work, an embedded optofluidic biosensing platform is proposed, which consists of asymmetrical core-offset optical fiber (ACOOF) structure, microfluidic chip and photoelectric detection system. In particular, by introducing the concept of optical fiber bridge, an ACOOF structure is designed to improve the optical coupling efficiency and reduce the limit of detection (LOD) of the developed sensor. On this basis, the human epidermal growth factor (EGF) was detected by constructing optofluidic chip and enzyme-linked immunosorbent assay (ELISA) kit. The LOD was 0.587 pg/mL. Leveraging the developed biosensing platform integrated with our chip, we achieved trace analysis with high specificity, as demonstrated by the measurement of interleukin 1α (IL-1α) with a low LOD of 43.3 fg/mL. Monitoring these two biomarkers is important for early cancer diagnosis. Beyond this, this novel platform has the potential for a range of applications, including disease diagnosis and management, bioanalysis, and health and environmental monitoring, with a focus on alternative biomarker targets.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100716"},"PeriodicalIF":5.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

“All-on-a-Tube” POCT of Salmonella in large-volume sample 大容量样本中沙门氏菌的 "全试管 "POCT检测

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2024-11-10 DOI: 10.1016/j.sbsr.2024.100712

Lei Wang, Nana Jin, Meixuan Li, Jianhan Lin

{"title":"“All-on-a-Tube” POCT of Salmonella in large-volume sample","authors":"Lei Wang, Nana Jin, Meixuan Li, Jianhan Lin","doi":"10.1016/j.sbsr.2024.100712","DOIUrl":"10.1016/j.sbsr.2024.100712","url":null,"abstract":"<div><div>Point-of-care testing (POCT) of pathogenic bacteria at low concentrations is vital to early warning of bacterial contaminations. A disposable centrifuge tube was reconstructed in this study for sensitive <em>Salmonella</em> detection in large-volume samples, where the entire bacterial detection progress from separation to detection, was performed within the tube. The bottom of this centrifuge tube was assembled with a flexible stirrer containing a pair of circular magnets, which was rapidly rotated using a DC motor to produce the vortex for simultaneous mixing and capture of target bacteria. Besides, immune manganese dioxide nanoflowers were synthesized and used to label target bacteria, followed by mimicking catalyze colorless TMB substrate into blue TMBox product. The product image was captured and analyzed by a smartphone App to quantitatively determine the target bacteria. This POCT centrifuge tube effectively achieved a separation efficiency of approximately 80 % for target bacteria from a 10 mL sample, enabling the detection of target bacteria within the range of 1.3 × 10<sup>1</sup> to 1.3 × 10<sup>4</sup> CFU/mL in 1 h, with a low detection limit of 13 CFU/mL. More importantly, this reconstructed centrifuge tube demonstrated its potential as a laboratory consumable for bacterial detection in routine screening owing to the features of low cost, easy operation and high integration.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100712"},"PeriodicalIF":5.4,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of flexible polyimide-based serpentine EMG sensor for AI-enabled fatigue detection in construction 设计基于柔性聚酰亚胺的蛇形肌电图传感器，用于人工智能建筑疲劳检测

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2024-11-08 DOI: 10.1016/j.sbsr.2024.100713

Yogesh Gautam, Houtan Jebelli

{"title":"Design of flexible polyimide-based serpentine EMG sensor for AI-enabled fatigue detection in construction","authors":"Yogesh Gautam, Houtan Jebelli","doi":"10.1016/j.sbsr.2024.100713","DOIUrl":"10.1016/j.sbsr.2024.100713","url":null,"abstract":"<div><div>Physical fatigue and musculoskeletal disorders are critical health issues for construction workers, stemming from repetitive motions, heavy lifting, and awkward postures. These factors compromise worker well-being, productivity, and safety while increasing the risk of accidents and long-term health problems. Recent advancements in wearable health monitoring technology offer potential solutions, but current sensors encounter significant challenges in the dynamic construction environment. These include inadequate skin contact, increased contact impedance, and vulnerability to motion artifacts all of which degrade signal quality and reduce the accuracy of fatigue detection. This paper develops a fractal-based, flexible sensor for enhanced adaptability and accurate fatigue estimation. Finite element analysis compared five space-filling designs, with the serpentine curve exhibiting the highest contact area and lowest strain, making it the preferred choice for fabrication. Evaluations demonstrated significant improvements in signal-to-noise ratio (SNR) and motion artifact reduction, with the newly developed sensor achieving a 37 % to 59 % SNR improvement over commercial electrodes across different muscle groups. The developed flexible sensor was integrated with a fatigue-detecting framework based on a vision transformer model which provided an average accuracy of 87 % for fatigue detection. The developed sensor significantly enhances EMG signal quality and reliability, promising improved health monitoring and safety for construction workers.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100713"},"PeriodicalIF":5.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular displacement approach for the electrochemical detection of protein-bound propofol 电化学检测蛋白结合型异丙酚的分子置换方法

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2024-11-07 DOI: 10.1016/j.sbsr.2024.100710

David C. Ferrier, Janice Kiely, Richard Luxton

{"title":"Molecular displacement approach for the electrochemical detection of protein-bound propofol","authors":"David C. Ferrier, Janice Kiely, Richard Luxton","doi":"10.1016/j.sbsr.2024.100710","DOIUrl":"10.1016/j.sbsr.2024.100710","url":null,"abstract":"<div><div>Propofol is one of the principal drugs used for the sedation of patients undergoing mechanical ventilation in intensive care units. The correct dosage of such sedative drugs is highly important, but current methods of determining infusion rates are limited and there is a lack of suitable methods for directly determining patient blood propofol concentrations. A significant challenge for the development of propofol sensors is that propofol demonstrates very high protein binding, leading to a low free fraction in blood. Here we present a method for improving the efficacy of an electrochemical propofol sensor by increasing the free fraction via a molecular displacement approach. When used in conjunction with a carbon nanotube/graphene oxide/iron oxide nanoparticle functionalised screen-printed electrode, it was found that this approach dramatically improved the sensor's sensitivity towards propofol. Ibuprofen was found to be the most effective displacement agent, with an optimal concentration of 30 mM. The resultant sensitivity was 2.82 nA/μg/ml/mm<sup>2</sup> with a coefficient of variation of 0.07, and the limit of detection was 0.2 μg/ml. This approach demonstrates high specificity towards drugs commonly administered to intensive care patients.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100710"},"PeriodicalIF":5.4,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosensor for integrin inhibition of mammalian cell adhesion and migration using micropatterned cell culture substrate and retroreflective optical signaling 利用微图案细胞培养基底和逆反射光学信号抑制哺乳动物细胞粘附和迁移的整合素生物传感器

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2024-11-06 DOI: 10.1016/j.sbsr.2024.100714

Kyung Won Lee , Eun Kyeong Yang , Duc Long Nguyen , Soo A Park , Moon Suk Kim , Jae-Ho Kim , Min Young Lee , Hyun C. Yoon

{"title":"Biosensor for integrin inhibition of mammalian cell adhesion and migration using micropatterned cell culture substrate and retroreflective optical signaling","authors":"Kyung Won Lee , Eun Kyeong Yang , Duc Long Nguyen , Soo A Park , Moon Suk Kim , Jae-Ho Kim , Min Young Lee , Hyun C. Yoon","doi":"10.1016/j.sbsr.2024.100714","DOIUrl":"10.1016/j.sbsr.2024.100714","url":null,"abstract":"<div><div>Integrins are a family of transmembrane receptors that play a crucial role in cell adhesion and migration. Integrins can uniquely transduce biochemical signals bidirectionally across the membrane and physically link the cell-cell and cell-extracellular matrix (ECM) with ligand bonds. The arginyl-glycyl-aspartic acid (RGD) peptide motif is present in the ECM as a minimal recognition sequence for integrins. To leverage this property in cell-based therapy, RGD variants, such as cyclic-type RGDfK (c(RGDfK)), which share a similar structure with RGD but exhibit a higher affinity for integrins, have been developed. However, because most evaluation methods for newly developed RGD variants focus on affinity strength, tools for cellular effects are required. In this study, we developed a new platform that integrates micropatterned three-dimensional cell culture substrates with a non-spectroscopic optical analysis system to quantitatively analyze the effects of RGD variants on cell adhesion and migration. The specially micropatterned substrate provides a cell adhesive and migration area to provide a restricted analysis area. Owing to the characteristics of retroreflective Janus particles (RJPs), a non-spectroscopic optical analysis system provides long-term stable optical verification properties and a simple optical setup. These techniques were integrated to quantitatively determine the integrin inhibitory effect of various concentrations of RGD variant. To demonstrate the efficacy of the developed cellular level RGD variant testing platform, the model cell line L929 fibroblast and model RGD variant c(RGDfK) were analyzed ranging from 0 to 10 μM. The results showed that the developed system could effectively and quantitatively analyze the effects of RGD variants on cells across various concentrations.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100714"},"PeriodicalIF":5.4,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in electrochemiluminescence-based sensors for ultra-sensitive pesticide residue detection 基于电化学发光的超灵敏农药残留检测传感器的研究进展

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2024-11-04 DOI: 10.1016/j.sbsr.2024.100708

Irkham , Chico Permana Putra , Clianta Yudin Kharismasari , Salma Nur Zakiyyah , Isnaini Rahmawati , Ivandini Tribidasari Anggraningrum , Wulan Tri Wahyuni , Giovanni Valenti , Francesco Paolucci , Yeni Wahyuni Hartati

{"title":"Advancements in electrochemiluminescence-based sensors for ultra-sensitive pesticide residue detection","authors":"Irkham , Chico Permana Putra , Clianta Yudin Kharismasari , Salma Nur Zakiyyah , Isnaini Rahmawati , Ivandini Tribidasari Anggraningrum , Wulan Tri Wahyuni , Giovanni Valenti , Francesco Paolucci , Yeni Wahyuni Hartati","doi":"10.1016/j.sbsr.2024.100708","DOIUrl":"10.1016/j.sbsr.2024.100708","url":null,"abstract":"<div><div>Pesticides are chemical substances utilized to prevent, control, and eradicate pests or organisms detrimental to crops, playing a pivotal role in minimizing agricultural losses and enhancing yields. However, improper pesticide use can lead to residue accumulation in the surrounding environment, including agricultural products, soil, and water. Detecting pesticide residues in the environment has become imperative. While various techniques such as chromatography, spectrophotometry, optical sensors, and electrochemical biosensors have been reported for pesticide residue detection, they face limitations such as large instrument size and low sensitivity. Currently, electrochemiluminescence (ECL) methods have been developed for pesticide detection. ECL methods can be combined with biosensor techniques or other molecule recognition element to enhance selectivity and specificity. ECL combines controllable electrochemical reaction properties with high photokinetic sensitivity. It can detect analytes with low detection limits (down to pM levels), ensuring rapid measurements and requiring minimal sample volumes for analysis. This literature review discusses the progress in research on detecting pesticide residues using ECL methods. The utilization of ECL, with its unique capabilities, signifies a promising avenue for addressing the shortcomings of traditional detection methods and advancing the field of pesticide residue analysis.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"46 ","pages":"Article 100708"},"PeriodicalIF":5.4,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0