使用少量全血检测白细胞衍生次氯酸盐离子的简化光学监测系统

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research Pub Date : 2025-02-01 DOI:10.1016/j.sbsr.2024.100722

Kozo Takeuchi , Toshiyuki Miwa , Takayuki Sato , Takahiro Shikayama , Hiroshi Tsuchiya , Kimiko Kazumura

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引用次数: 0

摘要

在这里，我们开发了两个简化的光学系统，FLP-H3200和FLP-H4200，旨在通过荧光（FL-OCl−）检测白细胞衍生的次氯酸盐离子，仅使用3微升全血。首先，新系统采用成本较低的光电二极管作为光电探测器，而不是之前系统CFL-H2200中的光电倍增管。该系统具有针对血液测量流控芯片优化的简化光学配置，能够有效聚焦所需的FL-OCl -信号，并获得与CFL-H2200相当的信号强度。这些改进使新系统比CFL-H2200更紧凑、更轻、更经济。此外，在FLP-H4200中，激发光源（发光二极管）的强度设置高于FLP-H3200，进一步增强了FL-OCl−信号。使用荧光试剂荧光素，我们证实了新系统的荧光灵敏度与CFL-H2200相当。最后，使用FLP-H3200和FLP-H4200的血液分析数据显示高度相关。本研究通过提供使用全血监测白细胞活动的方便和有效的工具，拓宽了临床检查的范围。

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Simplified optical monitoring systems for detecting leukocyte-derived hypochlorite ions using small amounts of whole blood

Here, we developed two simplified optical systems, FLP-H3200 and FLP-H4200, designed to detect leukocyte-derived hypochlorite ions via fluorescence (FL-OCl⁻) using only three microliters of whole blood. First, the new systems employed a lower-cost photodiode as the photodetector instead of a photomultiplier tube in their preceding system CFL-H2200. The systems featured a simplified optical configuration optimized for the blood measurement fluidic chip, enabling efficient focusing of the desired FL-OCl⁻ signal and obtaining a signal intensity comparable to that obtained with CFL-H2200. These improvements made the new systems more compact, lightweight, and cost-effective than CFL-H2200. Additionally, in FLP-H4200, the intensity of the excitation light source, the light-emitting diode, was set higher than that in FLP-H3200, further enhancing the FL-OCl⁻ signal. Using the fluorescent reagent fluorescein, we confirmed that the fluorescence sensitivity of the new systems was comparable to that of CFL-H2200. Finally, blood analysis data using FLP-H3200 and FLP-H4200 showed a high correlation. This study broadens the scope of clinical examinations by providing accessible and efficient tools for monitoring leukocyte activity using whole blood.

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来源期刊

Sensing and Bio-Sensing Research Engineering-Electrical and Electronic Engineering

CiteScore

10.70

自引率

3.80%

发文量

审稿时长

87 days

期刊介绍： Sensing and Bio-Sensing Research is an open access journal dedicated to the research, design, development, and application of bio-sensing and sensing technologies. The editors will accept research papers, reviews, field trials, and validation studies that are of significant relevance. These submissions should describe new concepts, enhance understanding of the field, or offer insights into the practical application, manufacturing, and commercialization of bio-sensing and sensing technologies. The journal covers a wide range of topics, including sensing principles and mechanisms, new materials development for transducers and recognition components, fabrication technology, and various types of sensors such as optical, electrochemical, mass-sensitive, gas, biosensors, and more. It also includes environmental, process control, and biomedical applications, signal processing, chemometrics, optoelectronic, mechanical, thermal, and magnetic sensors, as well as interface electronics. Additionally, it covers sensor systems and applications, µTAS (Micro Total Analysis Systems), development of solid-state devices for transducing physical signals, and analytical devices incorporating biological materials.