Sensing and Bio-Sensing Research最新文献_第9页

ATP-enhanced platinum nanoparticle's peroxidase activity in alkaline environment for simplifying alkaline phosphatase detection atp增强碱性环境下铂纳米颗粒过氧化物酶活性，简化碱性磷酸酶检测

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-04-29 DOI: 10.1016/j.sbsr.2025.100798

Lu Dang , Aoxue Zhang , Jinqing Li , Jin Cao , Xiangke Rong , Minyang Su , Baoji Du , Yazhong Bu

{"title":"ATP-enhanced platinum nanoparticle's peroxidase activity in alkaline environment for simplifying alkaline phosphatase detection","authors":"Lu Dang , Aoxue Zhang , Jinqing Li , Jin Cao , Xiangke Rong , Minyang Su , Baoji Du , Yazhong Bu","doi":"10.1016/j.sbsr.2025.100798","DOIUrl":"10.1016/j.sbsr.2025.100798","url":null,"abstract":"<div><div>In recent years, a new method has been developed for the quantitative detection of alkaline phosphatase (ALP) based on the signal-off principle using oxidase or peroxidase nanozymes. However, the nanozymes used usually lose their activity under alkaline conditions, making it difficult to match the pH for ALP-catalyzed hydrolysis. Consequently, this detection method requires additional pH adjustment, which complicates the detection process and makes it prone to errors. In this study, we explored the enhancing effect of adenosine triphosphate (ATP) on the peroxidase (POD) activity of platinum nanoparticles formed with different protectants (Pt-CS NPs, Pt-PVP NPs, and Pt-BSA NPs) under neutral and alkaline conditions. It was found that ATP enhanced the POD activity of positively charged Pt-CS NPs the most, manifested as a significant increase in the maximum reaction rate (V<sub>max</sub>). Based on the enhanced alkaline catalytic effect of ATP on Pt-CS NPs, we used Pt-CS/ATP as a new peroxidase nanozyme system. This system can catalyze at pH 8.5, enabling the detection of ALP without the need for pH adjustment. The research shows that the linear range of this detection system is 5–120 U/L, and the detection limit is 0.5 U/L. This study confirms that ATP can enhance the catalytic activity of platinum nanoparticles under neutral and alkaline conditions, providing a feasible solution for the direct detection of ALP under alkaline conditions using nanozymes.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"48 ","pages":"Article 100798"},"PeriodicalIF":5.4,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric sensing of Au3+ ions in aqueous media: A xanthene-based probe for rapid and visual detection 水介质中Au3+离子的比色感应：用于快速和视觉检测的基于杂蒽的探针

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-04-29 DOI: 10.1016/j.sbsr.2025.100799

Nuriye Tuna Subasi

{"title":"Colorimetric sensing of Au3+ ions in aqueous media: A xanthene-based probe for rapid and visual detection","authors":"Nuriye Tuna Subasi","doi":"10.1016/j.sbsr.2025.100799","DOIUrl":"10.1016/j.sbsr.2025.100799","url":null,"abstract":"<div><div>Ionic gold species play a pivotal role in various chemical and biological systems due to their unique properties. However, even trace amounts of Au<sup>3+</sup> ions (as low as 200 μM) can pose significant toxicity risks to living organisms, underscoring the need for accurate and rapid detection methods. In this study, we present the design and characterization of a novel colorimetric sensor, TS-Xan, specifically developed for Au<sup>3+</sup> ion detection. TS-Xan, a xanthene-derived probe, functions as a reversible sensor. Its molecular structure was characterized using nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry (HRMS). The sensor exhibited excellent sensitivity toward Au<sup>3+</sup> ions, inducing a distinct colorimetric shift from yellow to violet, visible to the naked eye in aqueous media. UV–vis absorption studies revealed the formation of a new absorption band at 570 nm, corresponding to the TS-Xan-Au<sup>3+</sup> complex. HRMS analysis further elucidated the binding stoichiometry, confirming a 2:1 (TS-Xan:Au<sup>3+</sup>) complex formation. To enhance its practical applicability, TS-Xan-coated test strips were fabricated, demonstrating exceptional sensitivity and reliability for on-site Au<sup>3+</sup> detection. This work introduces a promising and user-friendly approach for gold ion sensing, with potential applications in environmental monitoring, industrial waste analysis and bioanalytical fields.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"48 ","pages":"Article 100799"},"PeriodicalIF":5.4,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aptamer-based dual-enzyme, amplification-free biosensor integrating CRISPR-Cas12a and Exo III for sensitive detection of ATP 基于核酸适配体的双酶，无扩增生物传感器整合CRISPR-Cas12a和Exo III，用于ATP的敏感检测

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-04-29 DOI: 10.1016/j.sbsr.2025.100795

Baolin Li , Yaxin Huang , Zixin Zhu , Min Zhong , Guangrong Li , Jinbo Liu

{"title":"Aptamer-based dual-enzyme, amplification-free biosensor integrating CRISPR-Cas12a and Exo III for sensitive detection of ATP","authors":"Baolin Li , Yaxin Huang , Zixin Zhu , Min Zhong , Guangrong Li , Jinbo Liu","doi":"10.1016/j.sbsr.2025.100795","DOIUrl":"10.1016/j.sbsr.2025.100795","url":null,"abstract":"<div><div>Adenosine triphosphate (ATP) plays a critical role in cellular energy metabolism, acting as the energy source for many biological reactions. Abnormal fluctuations in ATP levels are directly linked to various diseases such as malignancies, bacterial infections, and cardiovascular disorders, making early-stage disease detection through rapid, highly sensitive ATP measurement essential. The current methods for ATP detection suffer from drawbacks such as complex procedures, reliance on expensive instruments, and prolonged reaction times. To this end, we have developed a novel biosensor named ATP Output Sensor Activated by CRISPR (AOSAC).This biosensor employs aptamer-based specific recognition combined with a dual-enzyme, amplification-free system involving Exo III and CRISPR-Cas12a for ATP detection. This sensor operates through an aptamer in a closed duplex structure, upon binding ATP, releases a trigger strand. This strand displaces an activation chain, which in turn interacts with Cas12a's crRNA, initiating trans-cleavage activity that cleaves a single-stranded DNA probe to produce a fluorescent signal. Concurrently, the activation chain's release sites are targeted by Exo III, enhancing signal amplification without the need for complex nucleic acid amplification, thus providing a highly selective, rapid detection method. This technique offers a linear detection range from 0 nM to 20 μM with a detection limit of 44.2 nM, presenting a novel strategy for signal amplification and high specificity in distinguishing ATP from its analogues.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"48 ","pages":"Article 100795"},"PeriodicalIF":5.4,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A one-pot, one-step CRISPR-AapCas12b-based platform for sensitive and specific detection of Klebsiella pneumoniae 基于crispr - aapcas12b的一锅一步灵敏特异性检测肺炎克雷伯菌平台

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-04-29 DOI: 10.1016/j.sbsr.2025.100797

Juan Zhou , Zirong Wu , Lei Yu , Xiaolan Huang , Fei Xiao , Yu Zhang , Linglong Wan , Jin Fu , Nan Jia , Min Chen , Chunrong Sun , Zhaomin Feng , Yi Wang

{"title":"A one-pot, one-step CRISPR-AapCas12b-based platform for sensitive and specific detection of Klebsiella pneumoniae","authors":"Juan Zhou , Zirong Wu , Lei Yu , Xiaolan Huang , Fei Xiao , Yu Zhang , Linglong Wan , Jin Fu , Nan Jia , Min Chen , Chunrong Sun , Zhaomin Feng , Yi Wang","doi":"10.1016/j.sbsr.2025.100797","DOIUrl":"10.1016/j.sbsr.2025.100797","url":null,"abstract":"<div><div><em>Klebsiella pneumoniae</em> (<em>K. pneumoniae</em>) was a prevalent nosocomial pathogen and a major cause of neonatal sepsis, highlighting the critical need for simple, rapid, sensitive and reliable diagnosis for <em>K. pneumoniae</em> infection. While CRISPR-based diagnostics provide a transformative technology to nucleic acid detection, current CRISPR-based methods often rely on PAM-dependent sequences, involve multiple steps, and are hampered by cumbersome manual operation, sequence limitations and the risk of aerosol contamination. To address these challenges, we developed a novel one-pot, one-step, PAM-independent CRISPR-Cas12b-based detection platform for rapid, sensitive and specific detection of <em>K. pneumoniae</em>, termed CRISPR-KP. This assay integrates multiple cross displacement amplification (MCDA) with CRISPR-Cas12b system in a single reaction mixture, employing MCDA for target nucleic acid amplification and the trans-cleavage activity of CRISPR-Cas12b system for amplification signal decoding. This one-pot assay achieves a remarkable limit of detection 10 fg (∼1.6 copies) of <em>K. pneumoniae</em> DNA within a 40-min isothermal reaction at 59 °C, eliminating the need for complex multi-step procedures. CRISPR-KP demonstrated comparable diagnostic efficiency to the clinical standard method in bronchoalveolar lavage fluid (BALF) samples, with reduced turnaround time and minimal manual operation, highlighting the excellent reliability and accuracy of our assay. Taken together, the CRISPR-KP assay offers a promising diagnostic tool for <em>K. pneumoniae</em> detection in centralized laboratories, point-of-care settings, and primary healthcare institutions.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"48 ","pages":"Article 100797"},"PeriodicalIF":5.4,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an electrochemical aptasensor for cocaine detection using CuFe2O4 and electroreduced graphene oxide 基于CuFe2O4和电还原氧化石墨烯的可卡因检测电化学感应传感器的研制

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-04-29 DOI: 10.1016/j.sbsr.2025.100796

Fatemeh Hamdi, Mahmoud Roushani

{"title":"Development of an electrochemical aptasensor for cocaine detection using CuFe2O4 and electroreduced graphene oxide","authors":"Fatemeh Hamdi, Mahmoud Roushani","doi":"10.1016/j.sbsr.2025.100796","DOIUrl":"10.1016/j.sbsr.2025.100796","url":null,"abstract":"<div><div>Cocaine is a type of narcotic used for medical purposes, such as local anesthesia in some surgeries. Cocaine diagnosis, which influences health and performance of human beings, is particularly essential in Clinical Toxicology and forensics. This underlines the importance of developing efficient and accurate tools to detect cocaine and track their behavior. Aptamers have been widely used as stable, efficient, and specific biorecognition molecules in biosensors. Herein, a CuFe<sub>2</sub>O<sub>4</sub> (CFO)/ ErGO (Electroreduced graphene oxide) based electrochemical biosensor was fabricated with aptamer for the detection of cocaine. This design increased the active surface area of the electrode that allows more sequences of Apt to be loaded on the surface of the modified electrode. CFO was synthesized using the hydrothermal method. Fe<sup>3+</sup> ions are placed on the Cu<sup>2+</sup> particles, leading to the production of CFO. Graphene oxide was deposited on it and subsequently reduced through electrochemical means to enhance the electrode surface. In the second step, CFO nanospheres were loaded onto the ErGO-modified electrode surface. The synergy between CFO and ErGO and the porosity of CFO increases conductivity and electron transport. Evaluating the calibration curve showed that the linear range and the detection limit were 0.5 fM- 30 fM and 30 fM- 0.7 nM, and 0.16 fM, respectively. The designed sensor showed excellent selectivity against interfering species. In addition, the performance of this biosensor in human serum samples as an actual sample was appropriately evaluated.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"48 ","pages":"Article 100796"},"PeriodicalIF":5.4,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a sensitive antibody-based label-free electrochemical immunosensors for breast cancer diagnosis 一种基于抗体的乳腺癌诊断无标记电化学免疫传感器的研制

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-04-21 DOI: 10.1016/j.sbsr.2025.100793

Saiedeh Agh Atabay , Hossein Ahmadzadeh , Parviz Norouzi , Somayeh Dianat , Kheibar Dashtian

{"title":"Development of a sensitive antibody-based label-free electrochemical immunosensors for breast cancer diagnosis","authors":"Saiedeh Agh Atabay , Hossein Ahmadzadeh , Parviz Norouzi , Somayeh Dianat , Kheibar Dashtian","doi":"10.1016/j.sbsr.2025.100793","DOIUrl":"10.1016/j.sbsr.2025.100793","url":null,"abstract":"<div><div>Early detection of human epidermal growth factor receptor 2 (HER2) is crucial for effective breast cancer treatment. This research focuses on the development of a sensitive, label-free electrochemical immunosensor for detecting HER2, which plays a key role in breast cancer diagnosis. The sensor employs a gold electrode functionalized with carboxylic acid molecules (11-mercaptoundecanoic acid, 3-mercaptopropionic acid), activated by coupling agents and modified with anti-HER2 antibodies. Key parameters such as pH, incubation time, and antibody concentration were optimized to enhance the immunosensor's performance. Using differential pulse voltammetry (DPV), the immunosensor achieved a limit of detection (LOD) of 2.3 fM within a range of 0.10 nM to 100 nM. To further improve detection limits and sensitivity, protein G (PG) was incorporated into the next iteration of the immunosensor. Again, a gold electrode was used as the main component, this time functionalized with L-cysteine (CYS) and PG. The PG-enhanced sensor exhibited improved sensitivity and a LOD of 0.51 fM, with a linear range from 1 fM to 0.10 nM. The sensor demonstrated high selectivity for HER2 and maintained performance in human serum samples. These results highlight its potential for rapid diagnostic applications in breast cancer monitoring.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"48 ","pages":"Article 100793"},"PeriodicalIF":5.4,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implementation of QKD-based system using Nano PCF for healthcare applications 在医疗保健应用中使用Nano PCF实现基于qkd的系统

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-04-19 DOI: 10.1016/j.sbsr.2025.100791

Ajay Kumar Vyas , Achyutesh Dixit , Subhashish Tiwari , Prem Shankar Mishra , Dileep Kumar Gupta , Sanjeev K Srivastava , Vijay Singh

{"title":"Implementation of QKD-based system using Nano PCF for healthcare applications","authors":"Ajay Kumar Vyas , Achyutesh Dixit , Subhashish Tiwari , Prem Shankar Mishra , Dileep Kumar Gupta , Sanjeev K Srivastava , Vijay Singh","doi":"10.1016/j.sbsr.2025.100791","DOIUrl":"10.1016/j.sbsr.2025.100791","url":null,"abstract":"<div><div>The present paper proposed a design of a Photonic Crystal Fiber (PCF) with a nano-scale coating of very thin gold dielectric rods in the first ring. In such a configuration, the single-ring PCF may act as a bridge or a connector between two multi-ring PCFs on both sides. This arrangement could be used in various optical communications where one is required to interface different types of PCF. Also, it can be used as a sensor for analyte as a blood sample for different patients. The envisioned sensor can identify unknown analytes either by passing through the metal surface or being applied to the outer surface of the metal layer. The single-ring PCF will be infused with blood samples from various patients to monitor changes in ligand and receptor concentrations with temperature. Additionally, this proposed sensor design features symmetrical elliptical gold-dielectric rods, with two small metal-rods in the first ring. These supplementary thin metal-dielectric rods in place of air holes enhance the generation of the evanescent field. This helps in adjusting the phase matching between the cores-guided mode and surface plasmon polaritons (SPP) mode. The sensing mechanism is based on the Quantum Key Distribution (QKD) technique and Surface Plasmon Resonance (SPR) occurring at the interfaces of dielectric-metal contacts. To estimate the dispersion and birefringence of the patterned PCF we employ the Finite Difference Time Domain (FDTD) method. Such research holds significance in the realm of biochemical sensing, offering potential advancements in this field. These simulation result is required to make its experimental validation and immediate applicability in clinical or diagnostic contexts to confirm the real-world feasibility and performance of the proposed PCFs structure in future works.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"48 ","pages":"Article 100791"},"PeriodicalIF":5.4,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A perspective on the selection and design of antimicrobial peptides for bacteria detection 细菌检测用抗菌肽的选择与设计

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-04-18 DOI: 10.1016/j.sbsr.2025.100794

Miral O. Sabry , Dan Li , Leon C.Z. Chan

{"title":"A perspective on the selection and design of antimicrobial peptides for bacteria detection","authors":"Miral O. Sabry , Dan Li , Leon C.Z. Chan","doi":"10.1016/j.sbsr.2025.100794","DOIUrl":"10.1016/j.sbsr.2025.100794","url":null,"abstract":"<div><div>Antimicrobial peptides (AMPs) are versatile biomolecules with unique properties, including specificity, stability, and ease of synthesis, making them promising candidates for bacteria detection in biosensors. This review explores the potential of AMPs as biorecognition elements, emphasizing their structural properties, interaction mechanisms with bacterial membranes, and their application in biosensing. AMPs offer advantages such as being less prone to bacterial resistance mechanisms and compatibility with various biosensor platforms, including optical and electrochemical sensors. Recent advancements in AMP design strategies, such as in-silico modeling and structural tailoring, in addition to immobilization techniques, are discussed, highlighting their role in enhancing AMP properties. Challenges and current research gaps affecting AMP efficacy in biosensors are critically examined and possible solutions are suggested and discussed. Future directions include the development of multiplexed AMP-based biosensors and integration into smart food packaging for real-time pathogen monitoring. By addressing current research gaps, AMPs could revolutionize bacterial detection methodologies across multiple fields, including medicine, food safety, and environmental monitoring.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"48 ","pages":"Article 100794"},"PeriodicalIF":5.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143869180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green voltammetric strategy for sensitive determination of paracetamol in pharmaceuticals and serum using alizarin red S-modified glassy carbon electrodes 用茜素红s修饰玻碳电极测定药物和血清中扑热息痛的绿色伏安策略

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-04-17 DOI: 10.1016/j.sbsr.2025.100792

Wudneh Girum, Adane Kassa

{"title":"Green voltammetric strategy for sensitive determination of paracetamol in pharmaceuticals and serum using alizarin red S-modified glassy carbon electrodes","authors":"Wudneh Girum, Adane Kassa","doi":"10.1016/j.sbsr.2025.100792","DOIUrl":"10.1016/j.sbsr.2025.100792","url":null,"abstract":"<div><div>This study introduces a highly sensitive electrochemical method for detecting paracetamol (PCT) in pharmaceutical tablets and human serum samples, utilizing a glassy carbon electrode modified with alizarin red S (poly (ARS)/GCE). PCT is one of the most widely used analgesic and antipyretic drugs; however, its overdose or prolonged use can lead to severe liver and kidney damage. Therefore, the development of sensitive and reliable methods for monitoring PCT levels in pharmaceutical formulations and biological fluids is crucial for ensuring drug safety and effective therapeutic monitoring. Characterization of the electrode confirmed that the surface modification with a conductive and electroactive polymer film (poly(ARS)) significantly enhanced the effective electrode surface area and reduced charge transfer resistance. Compared to the unmodified electrode, the modified electrode exhibited a well-resolved, irreversible redox peak at a significantly lower potential with a sixfold increase in current, highlighting the catalytic efficiency of the modifier toward PCT. The electrochemical behavior of PCT was analyzed via cyclic voltammetry and square wave voltammetry, revealing significantly enhanced sensitivity and selectivity due to the conductive polymer coating. Under optimized electrode condition square wave voltammetric current response of poly(ARS)/GCE showed linear dependence on concentration of 0.01–250.0 μM and an ultralow detection limit of 1.0 nM in phosphate buffer solution (pH 7.0). Analytical application on real samples confirmed the method's accuracy, achieving recovery rates of 98.8–100.3 % for pharmaceutical tablets and human blood serum, even in the presence of potential interferents. The developed method provides a cost-effective and robust alternative for PCT quantification, with superior performance compared to previously report electrochemical approaches.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"48 ","pages":"Article 100792"},"PeriodicalIF":5.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Telluride quantum dot-amplified immunosensor for interferon gamma – A TB antigen test biomarker 干扰素γ - A结核抗原检测生物标志物的碲化物量子点扩增免疫传感器

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-04-15 DOI: 10.1016/j.sbsr.2025.100788

Sixolile Mini , Kaylin Cleo Januarie , Marlon Oranzie , Candice Cupido , Kefilwe Vanessa Mokwebo , Usisipho Feleni , Emmanuel Iwuoha

{"title":"Telluride quantum dot-amplified immunosensor for interferon gamma – A TB antigen test biomarker","authors":"Sixolile Mini , Kaylin Cleo Januarie , Marlon Oranzie , Candice Cupido , Kefilwe Vanessa Mokwebo , Usisipho Feleni , Emmanuel Iwuoha","doi":"10.1016/j.sbsr.2025.100788","DOIUrl":"10.1016/j.sbsr.2025.100788","url":null,"abstract":"<div><div>Interferon-gamma (IFN-γ) is a cytokine (i.e. a microprotein involved in cell signaling and immunomodulation) which is known to be expressed in response to <em>Mycobacterium tuberculosis</em> (Mtb) infections. For this reason, IFN-γ has gained prominence as a diagnostic biomarker for TB antigen tests, such as Interferon-Gamma Release Assays (IGRAs). Recent research efforts have focused on improving the sensitivity of TB tests based on IFN-γ. In this study, a new and sensitive chalcogenide-based electrochemical IFN-γ immunosensor was developed. The chalcogenide is a 9.30 nm average diameter bimetallic copper zinc telluride (CuZnTe) quantum dot (QD) particles, which were stabilised by sequential triple-capping with thioglycolic acid (TGA), (3-mercaptopropyl)trimethoxysilane (MPS) and tetraethylorthosilicate (TEOS) (i.e. CuZnTe-TGA-MPS-TEOS QD). The morphologies of the quantum dots were determined by X-ray transmission electron microscopy (TEM) and small angle X-ray scattering spectroscopy (SAXS); while their structural characteristics were ascertained with Raman and X-ray diffraction (XRD) spectroscopic analyses. A gold disk electrode (AuE) surface was functionalized with CuZnTe-TGA-MPS-TEOS QD, anti-interferon-gamma antibody (Anti) and bovine serum albumin (BSA) to create a BSA/Anti/CuZnTe-TGA-MPS-TEOS QD/AuE amperometric immunosensor bioelectrode. The immunosensor exhibited a linear detection range (LDR) value of 2–20 pg/mL IFN-γ, a limit of detection (LOD) value of 0.82 pg/mL IFN-γ and was highly sensitive in human serum samples with recovery rates of 97–99 %.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"48 ","pages":"Article 100788"},"PeriodicalIF":5.4,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143869179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0