Sensing and Bio-Sensing Research最新文献_第8页

High-sensitivity creatine detection using doped ZnO nanoribbon biosensors: A density functional theory approach 利用掺杂ZnO纳米带生物传感器进行高灵敏度肌酸检测：密度泛函理论方法

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100767

A. Shahriyari , Sh. Bagherzadeh-Nobari , M. Yousefizad , N. Manavizadeh , H. Pourfarzad , Z. GolshanBafghi , H. Ahmadi , F. Gholipour

{"title":"High-sensitivity creatine detection using doped ZnO nanoribbon biosensors: A density functional theory approach","authors":"A. Shahriyari , Sh. Bagherzadeh-Nobari , M. Yousefizad , N. Manavizadeh , H. Pourfarzad , Z. GolshanBafghi , H. Ahmadi , F. Gholipour","doi":"10.1016/j.sbsr.2025.100767","DOIUrl":"10.1016/j.sbsr.2025.100767","url":null,"abstract":"<div><div>Determining creatine concentration in the liver and kidneys is crucial for diagnosing health status due to its role as a significant biomarker in biosynthesis and muscle energy metabolism. This study proposes a two-terminal device made of hydrogen-passivated armchair ZnO nanoribbons connected between ZnO nanoribbon electrodes to explore its potential as a creatine biosensor. Using Transiesta, Tbtrans, and Density Functional Theory (DFT) calculations, the study evaluates the sensing capabilities of doped AZnONR for the first time. It involves hydrogen passivation, detailed doping analysis, and the assessment of electronic structure and adsorption properties to optimize biosensor performance. Measurements of PDOS, transmission coefficient, conductance, charge transfer, and electrostatic potential elucidate detection mechanisms, focusing on Al, Ga, and N doping effects. Findings reveal that creatine adsorption leads to the disappearance of some states near the conduction band edge due to charge transfer from the Al-doped ZnO nanoribbon. The projected density of states and transmission shift to higher energies because of creatine's negative electrostatic gating effect, significantly reducing transmission at 0.7 eV. A gate voltage of 0.7 V drastically alters the device's conductance, enabling creatine detection with 99.9 % sensitivity. The study highlights Al-doped ZnO nanoribbons' potential as highly sensitive creatine biosensors with an adsorption energy of −3.07 eV.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100767"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated kirigami-patterned skin patch for multiplexed detection of inflammatory biomarkers along with transdermal drug delivery 一种集成的基里伽米图案皮肤贴片，用于多重检测炎症生物标志物以及透皮给药

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100772

Tanzila Noushin , Nafize Ishtiaque Hossain , Rhythem Tahrin , Md Najmul Islam , Shawana Tabassum

{"title":"An integrated kirigami-patterned skin patch for multiplexed detection of inflammatory biomarkers along with transdermal drug delivery","authors":"Tanzila Noushin , Nafize Ishtiaque Hossain , Rhythem Tahrin , Md Najmul Islam , Shawana Tabassum","doi":"10.1016/j.sbsr.2025.100772","DOIUrl":"10.1016/j.sbsr.2025.100772","url":null,"abstract":"<div><div>Detecting, monitoring, and managing chronic pain levels in real-time remains a critical and challenging aspect of medical practice. Chronic pain is associated with an increased production of inflammatory biomarkers, such as Interleukin-6 and Interleukin-10. Accurately detecting the levels of these biomarkers with a highly sensitive sensor can facilitate real-time monitoring of pain severity and enable the administration of appropriate medication based on the patient's needs. In this context, transdermal drug delivery offers a significant advantage in pain management by delivering targeted opioids, such as Fentanyl, to alleviate chronic pain in a non-invasive and long-term manner. This work presents a kirigami-patterned skin patch that combines multiplexed sensors with a drug delivery module to detect inflammatory biomarker levels in sweat with high sensitivity and precision, while also enabling on-demand drug delivery for pain relief. By correcting response variations caused by changes in body temperature and sweat pH, the device ensures accurate sensing while maintaining strain-insensitive performance—an essential feature for wearable sensors. This system has the potential to significantly impact healthcare by providing an innovative, reliable solution for chronic pain management.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100772"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical aptasensor for ultrasensitive detection of glycated hemoglobin (HbA1c) using gold-modified SPCE 金修饰SPCE超灵敏检测糖化血红蛋白（HbA1c）的电化学适体传感器

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100765

Serly Zuliska , Irkham , Salma Nur Zakiyyah , Yeni Wahyuni Hartati , Yasuaki Einaga , Iman Permana Maksum

{"title":"Electrochemical aptasensor for ultrasensitive detection of glycated hemoglobin (HbA1c) using gold-modified SPCE","authors":"Serly Zuliska , Irkham , Salma Nur Zakiyyah , Yeni Wahyuni Hartati , Yasuaki Einaga , Iman Permana Maksum","doi":"10.1016/j.sbsr.2025.100765","DOIUrl":"10.1016/j.sbsr.2025.100765","url":null,"abstract":"<div><div>Accurate monitoring of diabetes mellitus (DM) biomarkers, particularly glycated hemoglobin (HbA1c), is essential for assessing long-term blood glucose control, optimizing therapeutic strategies, and preventing diabetes-related complications. To enhance the efficiency of this monitoring, electrochemical aptasensors based on screen-printed carbon electrodes (SPCE) modified with gold nanoparticles (AuNP) have been developed. The results demonstrate that the electrodeposition technique produces a uniform AuNP distribution and significantly increases the current responses of K₃[Fe(CN)₆] compared to bare SPCE. Aptamer immobilization using a streptavidin-biotin system via linker-mediated attachment exhibits high affinity for HbA1c, as evidenced by a significant decrease in current upon target binding.The analytical performance of the aptasensor shows a low detection limit of 8.34 pg/mL, with a linear detection range of 1 to 10<sup>4</sup> pg/mL. Moreover, the aptasensor exhibits good selectivity for HbA1c over hemoglobin, glucose, and palmitic acid, with a selectivity value of 82.56 %. Stability tests indicate that the sensor retains 90.38 % of its activity after 70 days of storage, confirming its reliability for HbA1c detection. This study contributes to the advancement of effective electrochemical biosensors for diabetes monitoring, enabling early diagnosis and improved disease management.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100765"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon dots: Review of recent applications and perspectives in bio-sensing and biomarker detection 碳点：在生物传感和生物标志物检测中的最新应用及展望

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100771

Xeniya Terzapulo, Aiym Kassenova, Alissa Loskutova, Rostislav Bukasov

{"title":"Carbon dots: Review of recent applications and perspectives in bio-sensing and biomarker detection","authors":"Xeniya Terzapulo, Aiym Kassenova, Alissa Loskutova, Rostislav Bukasov","doi":"10.1016/j.sbsr.2025.100771","DOIUrl":"10.1016/j.sbsr.2025.100771","url":null,"abstract":"<div><div>Carbon Dots (CDs) are carbon-based nanoparticles with relatively strong and tunable fluorescence emission properties and relatively good photo stability, which are increasingly used in sensing and biosensing as reported in hundreds of research publications. There are 4 types of CD-based sensors or detection (CD-only, CD-based Enzymatic, CD-based Antigen-Antibody modified, CD-based Nucleic acid biosensors) that will be described and compared in this review. Such analytical parameters as Limit of Detection (LOD), linear range, Relative Standard Deviation (RSD) are compared and discussed in this review. Median and/or average LOD, RSD, Linear Range are calculated and presented in multiple tables, while LODs are tabulated from 135 references. Among these biosensors, CD-based Nucleic Acid biosensors and CD-based Antibody-Antigen biosensors show better sensitivity (lower LOD) when compared with CD-only and CD-based Enzymatic biosensors. Four main groups of detection techniques applicable, such as fluorescence, electrochemical, colorimetry, and electrochemiluminescence methods are considered and compared for CD-based Antibody-Antigen biosensors descriptions. Perspectives of machine learning methods and smart phones as read out devices for CD-based bio-sensors are outlined in the conclusion of the review. We also briefly described and illustrated with graphic examples/figures some basic phenomena related to CD-based sensing, including CD doping, up-conversion, modification and immobilization of CDs, quenching and FRET.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100771"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CRISPR/Cas12a electrochemical biosensing to detect pig mtDNA D-loop for ensuring food authenticity 利用CRISPR/Cas12a电化学生物传感技术检测猪mtDNA d环，确保食品的真实性

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100755

Muhammad Ihda H.L. Zein , Clianta Yudin Kharismasari , Ari Hardianto , Salma Nur Zakiyyah , Riezki Amalia , Mehmet Ozsoz , Mara Mirasoli , Irkham , Yeni Wahyuni Hartati

{"title":"A CRISPR/Cas12a electrochemical biosensing to detect pig mtDNA D-loop for ensuring food authenticity","authors":"Muhammad Ihda H.L. Zein , Clianta Yudin Kharismasari , Ari Hardianto , Salma Nur Zakiyyah , Riezki Amalia , Mehmet Ozsoz , Mara Mirasoli , Irkham , Yeni Wahyuni Hartati","doi":"10.1016/j.sbsr.2025.100755","DOIUrl":"10.1016/j.sbsr.2025.100755","url":null,"abstract":"<div><div>Ensuring safe and accurately labeled food products is crucial for communities worldwide. One significant concern in food safety is the unintended inclusion of pork in products, which impacts food labeling, religious dietary practices, legal standards, and public health. Detecting such contamination is challenging due to complex food processing, requiring advanced methods for accurate identification. A CRISPR/Cas12a-based electrochemical biosensor has garnered attention for its rapidity, portability, high sensitivity, and specificity, leveraging trans-cleavage activity for precise detection. This study utilized a modified electrode, SPCE/Ceria/STV/Biotin-ssDNA-Methylene Blue (MB), characterized by SEM/EDX and voltammetry methods to examine morphology and electrochemical behavior. Optimization using the Box-Behnken Design (BBD) yielded ideal conditions: 30 min STV incubation, 1.5 μM probe concentration, 240 min probe incubation, 0.1 % BSA concentration, 30 min target incubation, and 2 μL target volume. These parameters enabled the development of an ultrasensitive biosensor with detection and quantification limits of 4 fM and 71 fM, respectively. Operating within a range of 10 nM to 100 fM, the biosensor achieved 1.1 % RSD. This CRISPR-based biosensor successfully detected pig mtDNA in raw and processed meat samples, achieving a 100.82 % recovery rate for corned samples and maintaining stability for 14 days. These findings highlight the biosensor's potential as a rapid, stable, ultrasensitive, and ultraspecific alternative for food monitoring. Its robustness makes it particularly suitable for ensuring the authenticity of animal-derived food products susceptible to counterfeiting.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100755"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green synthesis of biocompatible fluorescent carbon dots from bitter gourd for effective metal sensing and biological applications 用苦瓜绿色合成生物相容性荧光碳点，用于有效的金属传感和生物应用

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100751

V. Renuga , X. Vinoliya , A. Dinesh , S. Suthakaran , Manikandan Ayyar , V. Mohanavel , Lalitha Gnanasekaran , Prabhu Paramasivam , R. Bousbih , M. Khalid Hossain , A. Rajendran , S. Santhoshkumar

{"title":"Green synthesis of biocompatible fluorescent carbon dots from bitter gourd for effective metal sensing and biological applications","authors":"V. Renuga , X. Vinoliya , A. Dinesh , S. Suthakaran , Manikandan Ayyar , V. Mohanavel , Lalitha Gnanasekaran , Prabhu Paramasivam , R. Bousbih , M. Khalid Hossain , A. Rajendran , S. Santhoshkumar","doi":"10.1016/j.sbsr.2025.100751","DOIUrl":"10.1016/j.sbsr.2025.100751","url":null,"abstract":"<div><div>Highly fluorescent Carbon dots (C-dots) were synthesized from the Bitter Gourd by simple hydrothermal method without the addition of any surfactant or additives. The optical properties, luminescence, phase formation and crystal structure were measured using UV–Visible spectroscopy (UV–Vis), Photoluminescence (PL), X-ray diffraction (XRD) and Transmission electron microscopy (TEM) by varying the medium from acidic to basic by changing its pH (2−12) and temperature range (180-300<sup>ο</sup>C) to assess the exact nature and properties of the synthesized C-dots. The synthesized C-dots have wide range of applications from metal sensing to bioimaging and optoelectronics. The antimicrobial properties of the prepared C-dots were also analyzed using <em>Klebsiellapneumoniae</em> and <em>Staphylococcus aureus</em> microbes and found that the prepared C-dots have better antimicrobial activity in both microbes with better efficiency due to its smaller size. The challenges and perspectives of the synthesized C-dots from Bitter Gourd were also discussed.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100751"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smart sensing of creatinine in urine samples: Leveraging Cu-nanowires/MoS2 quantum dots and machine learning 尿液样本中肌酐的智能传感：利用cu纳米线/MoS2量子点和机器学习

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2024.100727

Geethukrishnan , Paresh Prakash Bagde , Sammishra KH , Chandranath Adak , Rajendra P. Shukla , Kiran Kumar Tadi

{"title":"Smart sensing of creatinine in urine samples: Leveraging Cu-nanowires/MoS2 quantum dots and machine learning","authors":"Geethukrishnan , Paresh Prakash Bagde , Sammishra KH , Chandranath Adak , Rajendra P. Shukla , Kiran Kumar Tadi","doi":"10.1016/j.sbsr.2024.100727","DOIUrl":"10.1016/j.sbsr.2024.100727","url":null,"abstract":"<div><div>Serum creatinine (CRT) levels are key biomarkers for diagnosing, staging, and monitoring renal disease in clinical practice. In this work, copper nanowires (CuNW), and Molybdenum disulfide quantum dots (MSQD) modified glassy carbon electrode (GCE) were chosen to demonstrate the electrochemical detection of CRT in complex mixture and urine samples. The materials were characterized using various physical characterizations such as FESEM, XRD, UV, PL, and FT-Raman. The electrocatalytic activity of the sensor was investigated using cyclic voltammetry (CV), and differential pulse voltammetry (DPVs). Despite the elevated sensitivity and cost-effectiveness of electrochemical sensors, the performance of the sensors is constrained by the existence of interfering species that generate conflicting and overlapping electrochemical signatures. In order to address this issue, we implemented a machine learning (ML) approach to accurately quantify CRT levels in complex mixtures, as well as in urine samples. The ML algorithms employed are trained and tested on a large dataset, allowing them to effectively capture and analyze the variance in the electrochemical signatures, demonstrating the application of artificial intelligence. The proposed sensor exhibits linearity from 1.96 μM to 966.0 μM and shows the best performance in terms of limit-of-detection (LOD) of 2.3 μM in a complex mixture and 0.001 μM in real urine samples, with RMSE of 0.2 and 0.017 μM using artificial neural network and random forest ML models respectively. We anticipate that by further miniaturization of these sensors into point-of-care testing devices, renal diseases can be managed effectively.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100727"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrahigh effective mode area photonic crystal fibers with extremely low bending loss for long distance transmission application 具有超低弯曲损耗的超高有效模面积光子晶体光纤，适用于远距离传输

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2024.100734

Asif Shariar, Mehedi Hasan Milan

{"title":"Ultrahigh effective mode area photonic crystal fibers with extremely low bending loss for long distance transmission application","authors":"Asif Shariar, Mehedi Hasan Milan","doi":"10.1016/j.sbsr.2024.100734","DOIUrl":"10.1016/j.sbsr.2024.100734","url":null,"abstract":"<div><div>In this work, an extremely large mode area (LMA) photonic crystal fiber (PCF) design has been proposed having single mode operation. A full-vectorial finite element method (FEM) approach is considered with perfectly matched layer (PML) boundary for hybrid cladding with fluorine-doped silica holes in the cladding. An ultrahigh effective mode area can be achieved along with maintaining robust single mode operation. The hybrid cladding structure of the fiber makes it possible to exhibit lower bending loss and confinement loss. The proposed PCF design achieves an effective mode area of <span><math><mn>1727.55</mn><mi>μ</mi><msup><mi>m</mi><mn>2</mn></msup></math></span> at straight state and <span><math><mn>719.08</mn><mi>μ</mi><msup><mi>m</mi><mn>2</mn></msup></math></span> at bending state for a radius of <span><math><mn>30</mn><mi>cm</mi></math></span> at a wavelength of <span><math><mn>1550</mn><mi>nm</mi></math></span>. The proposed fiber also exhibits both an extremely low confinement loss of <span><math><mn>4.745</mn><mo>×</mo><msup><mn>10</mn><mrow><mo>−</mo><mn>4</mn></mrow></msup><mi>dB</mi><mo>/</mo><mi>Km</mi></math></span> and a bending loss of <span><math><mn>6.11</mn><mo>×</mo><msup><mn>10</mn><mrow><mo>−</mo><mn>4</mn></mrow></msup><mi>dB</mi><mo>/</mo><mi>Km</mi></math></span> at <span><math><mn>30</mn><mi>cm</mi></math></span> of bending radius at <span><math><mn>1550</mn><mi>nm</mi></math></span> wavelength. Moreover, the ultrahigh effective area of the fiber makes it possible to obtain an ultralower non-linear coefficient.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100734"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yerba mate tea mediated synthesis of nanoscale zero valent iron particles and their application in detection of Pb ions in water 马黛茶介导纳米级零价铁粒子的合成及其在水中铅离子检测中的应用

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2024.100728

L. Mampane , K. Moothi , O. Ntwampe , N. Moloto , G. Ndlovu , A. Jijana , P. Tetyana , N. Mphuthi , A. Ngqalakwezi , P. Shumbula , B. Ntsendwana , L. Sikhwivhilu

{"title":"Yerba mate tea mediated synthesis of nanoscale zero valent iron particles and their application in detection of Pb ions in water","authors":"L. Mampane , K. Moothi , O. Ntwampe , N. Moloto , G. Ndlovu , A. Jijana , P. Tetyana , N. Mphuthi , A. Ngqalakwezi , P. Shumbula , B. Ntsendwana , L. Sikhwivhilu","doi":"10.1016/j.sbsr.2024.100728","DOIUrl":"10.1016/j.sbsr.2024.100728","url":null,"abstract":"<div><div>Yerba-mate tea extracts were used to successfully synthesise nanoscale zero-valent iron (NZVI) particles. The tea extracts acted as both the reducing and capping agents. The morphological, optical and structural properties were characterized using Transmission Electron Microscope (TEM), X-ray Diffraction (XRD), UV–vis absorption spectroscopy and Fourier Transform Infrared (FTIR) spectroscopy, respectively. The XRD and FTIR confirmed a complete reduction of Fe ions forming nanoparticles with an average size of 3 to 3.5 nm synthesized at 25 °C as shown by TEM images, indicating enhanced electrocatalytic sites. The as-synthesized NZVI particles were immobilized on AuSPE and evaluated on the electrocatalytic behaviour using ferri/ferrocyanide as a redox probe. Thus, they were modified on the screen-printed carbon electrodes to fabricate an electrochemical sensor for Pb<sup>2+</sup> detection. The sensor was optimised to detect traces of Pb ions from 5 to 9 parts per billion concentrations. The surface concentration of the adsorbed electro-active film on the reduction half of AuSPE/NZVI modified electrodes was determined and estimated to be 1.32 × 10<sup>−10</sup> mol cm<sup>−2</sup><sub>.</sub> Moreover, the charge transfer coefficient of AuSPE/NZVI particles was estimated to be 5.43 × 10<sup>−17</sup> cm<sup>2</sup>s<sup>−1</sup>. The sensor gave a relatively low limit of detection 2.56 ppb for Pb<sup>2+</sup> ions. This was lower than most commercially available heavy metal detectors with a detection limit of 3.5 ppb.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100728"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cost-effective Amperometric Immunosensor for cardiac troponin I as a step towards affordable point-of-care diagnosis of acute myocardial infarction 具有成本效益的心脏肌钙蛋白I安培免疫传感器是向可负担得起的急性心肌梗死即时诊断迈出的一步

IF 5.4

Sensing and Bio-Sensing Research Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2024.100725

Niamh Docherty , Lilian Collins , Susan Pang , Ying Fu , Stuart Milne , Damion Corrigan

{"title":"Cost-effective Amperometric Immunosensor for cardiac troponin I as a step towards affordable point-of-care diagnosis of acute myocardial infarction","authors":"Niamh Docherty , Lilian Collins , Susan Pang , Ying Fu , Stuart Milne , Damion Corrigan","doi":"10.1016/j.sbsr.2024.100725","DOIUrl":"10.1016/j.sbsr.2024.100725","url":null,"abstract":"<div><div>Early detection of cardiac troponin I in blood is vital for acute myocardial infarction diagnosis. A low-cost thin film gold electrode array was used with affordable ELISA antibodies and reagents to fabricate two cardiac troponin I amperometric immunosensors. The HRP-labelled sandwich immunocomplex was constructed on the gold electrode surface, and chronoamperometry was used to quantify cTnI indirectly by measuring the amount of TMB<sup>+</sup> produced at the electrode surface. First, the system was evaluated using a physisorption approach to immobilise the capture antibody to the electrode with a 309 pg/mL LOD observed. Subsequently, a second sensor variant was produced using sulfo-LC-SPDP as a crosslinker to control antibody immobilisation, which resulted in an improved sensitivity with an LOD of 109 pg/mL. The chemisorption sensor outperformed the working range of the commercially available ELISA kit used (8000–125 pg/mL), demonstrating the power of enhanced antibody immobilisation and electrochemical detection for clinically relevant levels of cardiac troponin I. Amperometric immunosensors offer vital advantages including being cost-effective, simple to use, and compatible with commercially available reagents. These features make the sensor accessible to users and easy to manufacture. With further improvements to sensitivity and performance in complex samples, the sensor could be deployed to streamline acute myocardial infarction diagnosis and reduce the burden of chest pain patients on the healthcare system.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100725"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0