A highly sensitive and selective one-dimensional Ag@hydrogen-bonded organic framework-based biosensor for the detection of the prohibited food additive ractopamine

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research Pub Date : 2025-09-30 DOI:10.1016/j.sbsr.2025.100883

Mahmoud Roushani , Fatemeh Hamdi , Azam Zare Asadabadi , S. Jafar Hoseini

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引用次数: 0

Abstract

In this study, a novel and highly responsive biosensor was engineered for the identification of ractopamine (RAC), an illicit additive used to stimulate muscle development in farm animals. A hydrogen-bonded organic framework (HOF) was synthesized via a simple, economical, and environmentally friendly approach. To reinforce the electrochemical performance, silver nanoparticles (Ag NPs) were integrated into the HOF, thereby enlarging the electrode's active surface area and facilitating greater immobilization of RAC-specific aptamers (Apt). The successful immobilization of Apt RAC on the Ag@HOF-coated glassy carbon electrode (GCE) was validated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This sensing platform displayed a wide linear detection range from 0.01 fM - 0.25 nM, with an outstanding detection limit of 4 aM. Furthermore, the sensor demonstrated excellent specificity against potential interfering agents. Its reliable performance was also confirmed in complex biological samples, including milk and meat, indicating strong potential for practical, real-world applications.

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一种用于检测违禁食品添加剂莱克多巴胺的高灵敏度和选择性一维Ag@hydrogen-bonded有机框架生物传感器

在这项研究中，设计了一种新型的高响应生物传感器，用于识别莱克多巴胺（RAC），莱克多巴胺是一种用于刺激农场动物肌肉发育的非法添加剂。采用简单、经济、环保的方法合成了一种氢键有机骨架（HOF）。为了增强电化学性能，将银纳米粒子（Ag NPs）集成到HOF中，从而扩大电极的活性表面积，并促进rac特异性适配体（Apt）的固定化。利用循环伏安法（CV）和电化学阻抗谱法（EIS）验证了Apt RAC在Ag@HOF-coated玻碳电极（GCE）上的成功固定。该传感平台的线性检测范围为0.01 fM - 0.25 nM，检测限为4 aM。此外，该传感器对潜在干扰物表现出良好的特异性。在包括牛奶和肉类在内的复杂生物样品中也证实了其可靠的性能，表明其在实际应用中的强大潜力。

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来源期刊

Sensing and Bio-Sensing Research Engineering-Electrical and Electronic Engineering

CiteScore

10.70

自引率

3.80%

发文量

审稿时长

87 days

期刊介绍： Sensing and Bio-Sensing Research is an open access journal dedicated to the research, design, development, and application of bio-sensing and sensing technologies. The editors will accept research papers, reviews, field trials, and validation studies that are of significant relevance. These submissions should describe new concepts, enhance understanding of the field, or offer insights into the practical application, manufacturing, and commercialization of bio-sensing and sensing technologies. The journal covers a wide range of topics, including sensing principles and mechanisms, new materials development for transducers and recognition components, fabrication technology, and various types of sensors such as optical, electrochemical, mass-sensitive, gas, biosensors, and more. It also includes environmental, process control, and biomedical applications, signal processing, chemometrics, optoelectronic, mechanical, thermal, and magnetic sensors, as well as interface electronics. Additionally, it covers sensor systems and applications, µTAS (Micro Total Analysis Systems), development of solid-state devices for transducing physical signals, and analytical devices incorporating biological materials.