Sensing and Bio-Sensing Research最新文献

{"title":"Leak detection characteristics of point and line sensors for foundational data in smart safety management systems","authors":"Hyeon-Ki Lee ,&nbsp;Tae-Woong Park ,&nbsp;Hyeong-Soo Kim ,&nbsp;Hyeong-Joo Kim","doi":"10.1016/j.sbsr.2025.100841","DOIUrl":"10.1016/j.sbsr.2025.100841","url":null,"abstract":"<div><div>The leak detection characteristics of fifth-generation point sensors and film-type leak sensors were determined to provide foundational data for sensor system research. A comparative analysis of electrical conductivity revealed that sodium hydroxide had approximately 0.5 to 0.6 times the conductivity of sulfuric acid, indicating that alkaline solutions generally have a lower electrical conductivity than acidic solutions. Stable measurements were obtained by setting the minimum measured value to 10 mA. Under this condition, the minimum detectable concentrations were at least 0.2 % for point sensors and at least 0.4 % for line sensors. A 3D graph depicting changes in output values across the entire concentration range for sulfuric acid and sodium hydroxide showed greater variability in measurements for point sensors than line sensors. At high concentrations, the measurements for sulfuric acid were consistently lower than those for sodium hydroxide. The correlation between electrical conductivity and sensor results led to the development of predictive equations. For point sensor measurements, a quartic function was derived based on the concentrations of sulfuric acid and sodium hydroxide solutions, while for line sensor measurements, an exponential function was used. Both measured and predicted values confirmed that the minimum detectable concentrations were at least 0.2 % for point sensors and at least 0.4 % for line sensors. These findings provide valuable data for the development of advanced smart safety management systems.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"49 ","pages":"Article 100841"},"PeriodicalIF":5.4,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-enhanced graphene-gold hybrid metasurface sensor for high-precision terahertz detection of Isoquercitrin biomarkers","authors":"Jacob Wekalao ,&nbsp;Hussein A. Elsayed ,&nbsp;Haifa A. Alqhtani ,&nbsp;Mayi bin Jumah ,&nbsp;Mostafa R. Abukhadra ,&nbsp;Stefano Bellucci ,&nbsp;Amuthakkannan Rajakannu ,&nbsp;Ahmed Mehaney","doi":"10.1016/j.sbsr.2025.100842","DOIUrl":"10.1016/j.sbsr.2025.100842","url":null,"abstract":"<div><div>This study presents a sensor operating in the terahertz (THz) frequency range for the selective detection and quantification of isoquercitrin, a crucial flavonoid biomarker. Through optimization of graphene chemical potential (and geometric parameters, the sensor achieves exceptional sensitivity of 1000 GHz/RIU with a quality factor ranging from 7.849 to 8.000. The integration of machine learning algorithms, including an ensemble of Random Forest, Support Vector Machine, and Neural Network models, significantly enhances analytical capabilities with 98.7 % prediction accuracy and 2.3 μg/mL RMSE. The ML framework incorporates advanced spectral pre-processing with 95 % noise reduction, automated extraction of 127 spectral features, and real-time processing capabilities with sub-second response times (0.12 s). Electric field distribution analysis reveals optimal resonance at 0.68 THz with maximum field confinement, while the sensor demonstrates robust performance across varying incidence angles. The proposed system offers superior detection limits, high selectivity, and exceptional reliability with 95.3 % average prediction confidence, making it highly suitable for point-of-care diagnostics, nutraceutical quality control, and personalized health monitoring applications.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"49 ","pages":"Article 100842"},"PeriodicalIF":5.4,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetic screening of nucleic acid ligand libraries for hit identification, binding mode characterization, and sequence optimization","authors":"Mareike C. De Pascali ,&nbsp;Nico Dreymann ,&nbsp;Marcus M. Menger ,&nbsp;Ulrich Rant ,&nbsp;Wouter Engelen","doi":"10.1016/j.sbsr.2025.100840","DOIUrl":"10.1016/j.sbsr.2025.100840","url":null,"abstract":"<div><div>Binding and unbinding rates are important characteristics of molecular interactions as they dictate binding affinities and complex lifetimes. Particularly, the engineering and maturation of nucleic acid-based ligands for therapeutic or diagnostic purposes will greatly benefit from adopting kinetic characterization in screening efforts. Here we introduce a kinetic screening setup for nucleic acid ligands based on a fluorescence biosensor platform, allowing rapid selection and kinetic characterization of aptamer sequences from random or rationally designed mutant libraries. The modular, non-covalent approach allows for a rapid and cost-effective exchange of nucleic acid ligands and automated measurements of the association and dissociation of various analytes. We screened 846 unique biomolecular interactions covered by 1774 kinetic measurements, enabling us to map essential and non-essential nucleotides in two independent aptamers, evaluate the effects of sequence variations on binding kinetics, and provide lead sequences to introduce specificity in an inherently promiscuous small molecule-binding aptamer.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"49 ","pages":"Article 100840"},"PeriodicalIF":5.4,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-labeling strategy to enhance direct aptamer sensor sensitivity for detecting MUC1 tumor marker","authors":"Ashkan Koushanpour ,&nbsp;Edward J. Harvey ,&nbsp;Geraldine Merle","doi":"10.1016/j.sbsr.2025.100833","DOIUrl":"10.1016/j.sbsr.2025.100833","url":null,"abstract":"<div><div>Aptamers hold great potential for point-of-care diagnostics (POC), but the complexity of sensor architectures and poor sensitivities in detecting small molecules remain challenging. In this study, we present a simple but effective approach to enhance the sensitivity of the electrochemical ap-tamer-based (<em>E</em>-AB) sensors. The proposed aptamer was labeled by double redox tags through a lysine linker and incorporated with an optimized length of passivation layer, which cooperatively led to gain enhancement and thus higher sensitivity. The analytical performance of this <em>E</em>-AB sen-sor was measured and compared with a conventional E-AB sensor towards the detection of MUC1 in buffer and serum. Our study revealed the double-tagged aptamer with a lysine linker's superior performance, yielding a low 2.4 nM limit of detection (LOD) for MUC1 in buffer, with a wide lin-ear dynamic range (LDR) from 5.0 × 101 to 4.0 × 102 nM. In contrast, the conventional counterpart exhibited a tenfold higher LOD (25.7 nM). This innovative synthetic strategy addresses the limita-tions of the signal-to-noise ratio (S/N) and the need for higher sensitivity towards the detection of the tumor markers, which may hold promise for rapid simple-to-answer technology for P.O·C testing.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"49 ","pages":"Article 100833"},"PeriodicalIF":5.4,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an electrochemical biosensor based on MOF@AuNPs nanocomposite for early-stage evaluation of ovarian cancer via CA125 oncomarker in human serum samples","authors":"Ihsan Ibadi ,&nbsp;Abdolhossein Naseri ,&nbsp;Balal Khalilzadeh","doi":"10.1016/j.sbsr.2025.100838","DOIUrl":"10.1016/j.sbsr.2025.100838","url":null,"abstract":"<div><div>Among gynecologic cancers, ovarian cancer (OC) is one of the main causes of mortality. Therefore, early detection is essential for successful treatment. We were motivated to develop a label-free electrochemical biosensor for the prompt evaluation of OC through cancer antigen 125 (CA125) due to the insufficiency of the sensitivity of the available diagnostic techniques. To improve conductivity and sensitivity, this biosensor uses gallate-based metal organic framework (MIL-156 MOF) nanocomposite decorated with gold nanoparticles (AuNPs). Large-surface-area MIL-156 MOF was electrodeposited onto a glassy carbon electrode (GCE) and then decorated with AuNPs. The chronoamperometry (CHA) technique was used to carry out both electrodeposition steps. The characteristics of nanomaterials were assessed using EDX, SEM, and XRD methods. Here, an electrochemical biosensor based on MOF@AuNPs is developed for the evaluation of ovarian cancer using a biotinylated monoclonal CA125 antibody. The differential pulse voltammetry (DPV) technique was applied to perform the electrochemical measurements. The designed biosensor showed a low limit of detection (LOD = 7.185 nU/mL), a dynamic linear range (10–70 nU/mL), and desirable repeatability and reproducibility for CA125 detection. Clinical serum sample analysis demonstrated its ability to improve patient outcomes and confirmed its practical application. Biomedical research and clinical diagnostics could benefit from this robust and selective biosensor platform for screening approaches.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"49 ","pages":"Article 100838"},"PeriodicalIF":5.4,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wearable and wireless sEMG acquisition system based on the Internet of Medical Things","authors":"Manuela Gomez-Correa ,&nbsp;David Cruz-Ortiz ,&nbsp;Mariana Ballesteros","doi":"10.1016/j.sbsr.2025.100828","DOIUrl":"10.1016/j.sbsr.2025.100828","url":null,"abstract":"<div><div>The use and development of wearable systems, including the Internet of Things, have become very relevant in recent years. This type of technology has gained popularity in biomedical applications thanks to its advantages in remote patient monitoring and rehabilitation. The aim of the above is to generate greater access to health services, create personalized systems adapted to the needs of patients, and reduce the necessity of having specialized personnel constantly available while evaluating users. Therefore, the present work developed a portable and wireless surface electromyography (sEMG) system based on the Internet of Medical Things, named MUSS, for Muscle Sensing System. This system has eight wireless modules for biosignal recording, online acquisition, and sEMG signal processing. The system presents an innovative circuit that uses Wi-Fi communication for the online adjustment of sEMG parameters, such as gain, signal offset, and the implementation of digital filters to perform tests based on the application’s needs. Finally, the developed software allows the connection to the Cloud for remote control, information exchange between specialized personnel, and Cloud storage for greater accessibility. The system comparison with different commercial devices and a clinical device demonstrated the usability and advantages of the device developed, considering features such as signal-to-noise ratio, frequency peaks, energy, among others.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"49 ","pages":"Article 100828"},"PeriodicalIF":5.4,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wheat Germ Agglutinin physisorption on Love Surface Acoustic Wave sensor for biofluid analysis","authors":"Martin Millicovsky ,&nbsp;Luis Schierloh ,&nbsp;Pablo Kler ,&nbsp;Gabriel Muñoz ,&nbsp;Matias Machtey ,&nbsp;Martin Zalazar","doi":"10.1016/j.sbsr.2025.100830","DOIUrl":"10.1016/j.sbsr.2025.100830","url":null,"abstract":"<div><div>Biosensors play an essential role in the detection of biomarkers, enabling precise and early diagnosis as well as effective monitoring of diseases. However, conventional immobilization techniques for biosensors, such as covalent attachment and silanization, often require harsh chemical treatments that can alter the structure and activity of bioreceptors, limiting their effectiveness. As an alternative, physisorption offers a milder approach, preserving biomolecular activity by relying on non-covalent interactions. When combined with the Love Surface Acoustic Wave (LSAW) transducer—a highly sensitive technology for biosensing applications—physisorption presents a promising strategy for developing efficient biosensors. In this study, a gold-coated LSAW sensor was used to investigate the physisorption of Wheat Germ Agglutinin (WGA) lectin. A sensing system prototype was developed and characterized to assess sensor response across different WGA concentrations in a pH<!--> <!-->9 buffer aligned with WGA isoelectric point. The obtained biosensor was tested with human reflex tears at different dilution levels to evaluate the signal response of putative WGA-binding molecules. Results confirmed that physisorption is a robust and reliable immobilization technique. Moreover, integrating LSAW sensors with physisorption creates an innovative biosensing strategy, enabling high-sensitivity detection in complex biological fluids. This advancement underscores its significant potential for supporting tear-based diagnostics and expanding its applications in biomedical analysis.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"49 ","pages":"Article 100830"},"PeriodicalIF":5.4,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boron-doped diamond electrodes for electrochemical glucose detection via enzymatic and non-enzymatic approaches","authors":"Aqilla Irfa Muthia Rahma ,&nbsp;Rafa Radithya Swara ,&nbsp;Salma Nur Zakiyyah ,&nbsp;Clianta Yudin Kharismasari ,&nbsp;Ziping Zhang ,&nbsp;Yasuaki Einaga ,&nbsp;Irwan Kurnia ,&nbsp;Yeni Wahyuni Hartati ,&nbsp;Irkham","doi":"10.1016/j.sbsr.2025.100834","DOIUrl":"10.1016/j.sbsr.2025.100834","url":null,"abstract":"<div><div>The development of highly sensitive and reliable glucose sensors has driven significant interest in boron-doped diamond (BDD) electrodes due to their exceptional electrochemical properties. With superior chemical stability, a wide potential window, low background current, and excellent electrical conductivity, BDD provides an optimal platform for precise, stable, and interference-free glucose detection. It's remarkable fouling resistance and resilience against chemical degradation further enhance long-term reliability in both biological and laboratory settings. This article reviews recent advancements in BDD-based glucose sensors, focusing on surface modifications and electrode enhancements that improve performance of BDD for glucose detection. Both enzymatic and non-enzymatic detection strategies have been explored, demonstrating low detection limits, high sensitivity, and excellent stability. These innovations hold promise for a wide range of applications, including clinical diagnostics for diabetes, food and beverage industry quality control, and environmental monitoring, particularly in assessing glucose levels in water and soil, expanding the scope of BDD-based sensors.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"49 ","pages":"Article 100834"},"PeriodicalIF":5.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive miniaturized optical sensor for trace cyanide detection via supramolecular solvent microextraction of cyano-dithizone adduct","authors":"M.M. Dabi ,&nbsp;F.M. Alshareef ,&nbsp;H. Alwael ,&nbsp;T.N. Abduljabbar ,&nbsp;A.A. Alkhraije ,&nbsp;E.A. Assirey ,&nbsp;M.S. El-Shahawi","doi":"10.1016/j.sbsr.2025.100836","DOIUrl":"10.1016/j.sbsr.2025.100836","url":null,"abstract":"<div><div>The ultrasensitive detection of cyanide ions (CN⁻) at sub-trace levels in aqueous environments has emerged as an imperative analytical challenge, driven by its lethality for ecological systems and public health. The implementation of wavelength-dependent spectrochemical methodologies based on chromogenic adduct formation for CN⁻ quantification in aqueous media remains intrinsically challenging, owing to intrinsic self-absorbance, spectral convolution, and diffuse background scattering that collectively compromise analytical resolution and selectivity. In this study, a novel, eco-compatible, and analytically robust sample pretreatment protocol was devised for the ultra-trace extraction of CN⁻ ions from aqueous matrices, based on supramolecular solvent-based dispersive liquid–liquid microextraction (SM-DLLME) strategy, prior to miniaturized UV–visible spectrophotometric quantification. The supramolecular phase, comprising self-assembled reverse micelles of 1-octanol dispersed in tetrahydrofuran (THF), was spontaneously generated upon injection of the binary solvent mixture into CN⁻-containing aqueous solutions buffered at pH 10. Subsequent ultrasonic irradiation facilitated rapid micellization and dispersion, enabling the efficient partitioning of the hydrophobic neutral brownish-orange cyano-dithizone adduct [CN(H₂Dz)₂], centrifuged and extracted into the supramolecular phase. The chromogenic adduct was quantitatively assessed for CN⁻ ions at λ_max = 470 nm, with linearity across the concentration range 0.05–0.3 μg/mL (R² = 0.999). The acquired detection and quantification limits were 1.03 × 10⁻² and 4.01 × 10⁻² μg/mL, respectively. The sensor was implemented in spiked real water samples and yielded outstanding recoveries (100.3 ± 2.4 % to 101.8 ± 1.7 %), validated via the Student's <em>t</em>-test (t_exp = 1.5 &lt; t_tab = 2.78, <em>n</em> = 5, <em>P</em> = 0.05). Furthermore, the chemical equilibrium, stoichiometric configuration, and thermodynamic parameters governing formation of the [CN(H₂Dz)₂] adduct were systematically elucidated and assigned. The assay can assist as a talented substitute for CN⁻ detection in food, biogenic amines and food freshness. Incorporation of dithizone as a functional complexing agent markedly enhanced the sensing performance and selectivity of the established SM-DLLME system and thus enhanced the analytical scope for ultra-trace detection of CN⁻ ions in complex aqueous matrices. The proposed sample prep avoids the use of toxic organic solvents or complex nanomaterials, providing a direct and effective strategy for monitoring and quality control of CN⁻ ions in environmental water.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"49 ","pages":"Article 100836"},"PeriodicalIF":5.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silver nanoparticles prepared via an eco-friendly phytoextract method and their humidity sensing properties","authors":"M.V. Arularasu ,&nbsp;M. Sai Deiv Ramkumar ,&nbsp;Raghuraman Tarunprasad ,&nbsp;Packirisamy Vinitha","doi":"10.1016/j.sbsr.2025.100837","DOIUrl":"10.1016/j.sbsr.2025.100837","url":null,"abstract":"<div><div>In this work, a resistive-type thin film humidity sensor is developed using phytoextracted silver nanoparticles (Ag NPs) for first time. Initially, Ag NPs were synthesized by a sustainable approach using the aqueous extract of turmeric powder, in which plant biomaterials function as both reducing as well as capping agents. The Ag NPs structural, morphological and composition properties were characterized using X-ray diffraction (XRD), X-ray photoelectron spectrum (XPS), Fourier transform infrared spectrum (FT-IR), UV–Visible, High resolution scanning electron microscopy (HR-SEM) coupled with energy dispersive X-ray (EDX) spectrometer techniques. The XRD result revealed a significant diffraction peak appeared at 38.62°, 44.16°, 66.29°, 76.53° which correspondent to the (111), (200), (220) and (311) plane would confirm the crystalline nature with cubic phase structure of Ag NPs. The spherical shaped morphological nature has been confirmed by HR-SEM analysis. The UV–Visible spectra showing a characteristic peak of Ag NPs at an absorption maximum at 429 nm. The humidity sensing properties of Ag NPs have been exposed to a humidity range from 5 % to 98 % relative humidity (RH) and three different testing frequency (100, 500 and 1000 Hz). The obtained results confirmed that the optimum testing frequency is 500 Hz. Consequently, we observed fast response (28 s) and recovery (47 s), low hysteresis characteristic, and long-term stability (up to 30 days). Owing to this merit, the implemented sensor has been illustrated for a great application prospect in smart food packaging, breath analysis, non-contact sensing, and soil monitoring.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"49 ","pages":"Article 100837"},"PeriodicalIF":5.4,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}