Sensing and Bio-Sensing Research最新文献

{"title":"Electrocatalytic behaviour of self-assembled Cu-chitosan/f-MWCNT on glassy carbon electrode for detection of erythromycin in various samples","authors":"Prashu Jain,&nbsp;Sony Jagtap,&nbsp;Megha Chauhan,&nbsp;Ramani V. Motghare","doi":"10.1016/j.sbsr.2023.100568","DOIUrl":"https://doi.org/10.1016/j.sbsr.2023.100568","url":null,"abstract":"<div><p>Although antibiotics are class of compounds that are administered to fight bacterial infections in human and animals, its abuse is ensuing its occurrence in natural resources producing drug resistant strains, which in turn affect human health. Therefore, it is important to develop rapid and effective detection techniques for such antibiotics which are contaminating various natural resources. In this study, a novel electrochemical sensor was developed for determination of one of the most used macrolide antibiotic “Erythromycin” based on self- assembled cu-chitosan/f-MWCNT modified glassy carbon electrode. The negatively charged f-MWCNT holds the positively charged chitosan and Cu²⁺ ions, leading to formation of Cu-chitosan/f-MWCNT/GCE. FE-SEM, FT-IR, EDS as well as electrochemical methods such as CV, DPV and EIS were used to characterize formation of Cu-chitosan/f-MWCNT/GCE at every step of fabrication. From thermo-gravimetric analysis the composite was found to be stable up to 120 °C and further heating results in breakdown of the skeletal structure between 300 °C to 500 °C. Results from Cyclic voltammetry and electrochemical impedance spectroscopy indicated that after modification, the value of charge transfer resistance (Rct) decreased, and the electron transfer kinetics increased, significantly. In comparison to bare GCE, the Cu-chitosan/f-MWCNT/GCE displayed excellent electrocatalytic activity for the oxidation of erythromycin, as indicated by an increased oxidation peak current. The differential pulse peak current was linear for erythromycin concentration from 0.5 × 10⁻⁶ to 10 × 10⁻⁶ and 10 × 10⁻⁶ to 150 × 10⁻⁶M, with LOD 0.2 × 10⁻⁶M. The sensor demonstrated high selectivity, excellent stability, and impressive repeatability, during erythromycin determination. Thus, the proposed sensor demonstrates promising analytical applicability towards erythromycin detection in various samples.</p></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"41 ","pages":"Article 100568"},"PeriodicalIF":5.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1747627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensing a SARS-CoV-2 spike peptide using a titanium carbide-doped imprinted polymer-coated extended-gate field effect transistor","authors":"Mei-Hwa Lee ,&nbsp;Cheng-Chih Lin ,&nbsp;James L. Thomas ,&nbsp;Chen-Yuan Chen ,&nbsp;Chuen-Yau Chen ,&nbsp;Chien-Hsin Yang ,&nbsp;Hung-Yin Lin","doi":"10.1016/j.sbsr.2023.100577","DOIUrl":"10.1016/j.sbsr.2023.100577","url":null,"abstract":"<div><p>The <strong>COVID-19 pandemic</strong> of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has over 750 million confirmed cases globally and more than six million deaths. Several variants have been named and identified as variants of concern by the World Health Organization (WHO); these include the Delta and Omicron variants. This work demonstrates the integration of epitope-imprinted conductive polymers with extended gate field effect transistors for the sensitive detection of the covid spike protein. Peptides from the receptor-binding domain on the spike protein were synthesized and imprinted onto poly(aniline-<em>co</em>-3-aminobenzenesulfonic acid), poly(AN-<em>co</em>-MSAN), by electropolymerization. Doping the conductive polymer film with titanium carbide (Ti₂C) strengthened the electrochemical response approximately 1.5-fold. The FET platform not only amplified the electrochemical response about two-fold (compared with electrode-based sensing), but also lowered the sensing range for the SARS-CoV-2 spike protein subunit S1 (ncovS1) from 1.0 to 0.01 fg/mL.</p></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"41 ","pages":"Article 100577"},"PeriodicalIF":5.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214180423000296/pdfft?md5=0c995ff16ea753dc6f0703303e3f9f85&pid=1-s2.0-S2214180423000296-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44595341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent developments in electrochemical sensors based on graphene for bioanalytical applications","authors":"Abdulazeez Tunbosun Lawal","doi":"10.1016/j.sbsr.2023.100571","DOIUrl":"https://doi.org/10.1016/j.sbsr.2023.100571","url":null,"abstract":"<div><p>An effective and affordable technique for detecting a variety of substances of biological, medical, and environmental significance is electrochemical sensing (ECS). Due to their distinctive structures and abilities to offer robust electrocatalytic activity with little surface fouling, low cost, biocompatibility, and simple electron transfer kinetics, carbon materials-based electrodes like graphene (GR) and carbon nanotube (CNT) are frequently used for the development of electrochemical sensors. Since GR has a large specific surface area, it can host a large number of biomolecules and still have a decent detection sensitivity. Along with important developments in the synthesis, purification, conjugation, and biofunctionalization of GR, device integration technologies and nanofabrication of GR have evolved. Combinations of the aforementioned characteristics have accelerated the development of GR-based sensors for many critical bio-analyses. Along with important developments in the synthesis, purification, conjugation, and biofunctionalization of GR, device integration technologies and nanofabrication of GR have evolved. The rapid development of GR-based sensors for many critical bioanalyses with better detection sensitivity and selectivity has been facilitated by the combination of the aforementioned features. Direct electron transport between the enzyme and the active electrode region is made possible by the application of GR. It is possible to easily construct and execute on-site sensors for oxidases and dehydrogenases with improved selectivity thanks to the excellent electro-catalytic activities of GR on the redox reaction of hydrogen peroxide (H₂O₂) and nicotinamide adenine dinucleotides (NADH).</p><p>Since GR's oxidation of NADH, thiols, H₂O₂, and other interfering species occurs at low potentials, the use of GR-based electrodes effectively suppresses these species. The two benefits of GR-based sensors for use in clinical chemistry, food quality and control, wastewater treatment, and bioprocessing are their great resistance to electrode fouling and selectivity.</p></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"41 ","pages":"Article 100571"},"PeriodicalIF":5.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3447387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid detection of tetracycline residues at clinically relevant levels via a novel, inexpensive and analytically-sensitive magneto-immunoassay – A feasibility study","authors":"Owen J. Harrison ,&nbsp;Bertrand Monnier ,&nbsp;Ed Regan ,&nbsp;Dave West ,&nbsp;Hugh Ballantine Dykes ,&nbsp;Jeffrey S. Davey ,&nbsp;Janice Kiely ,&nbsp;Richard Luxton","doi":"10.1016/j.sbsr.2023.100566","DOIUrl":"https://doi.org/10.1016/j.sbsr.2023.100566","url":null,"abstract":"<div><p>Expensive, time-consuming and labour-intensive solvent-extraction and liquid-chromatography methods are the industry's current gold standard for antibiotic residue quantification. A novel immunoassay methodology and system for the rapid detection of clinically relevant levels of tetracycline residues found in food-producing animal tissues is described. Anti-tetracycline antibody-coated paramagnetic particles were used for the specific capture of tetracycline in spiked buffer (with and without a 1% pork muscle tissue suspension) and quantified via an analytically-sensitive in-house magnetometer instrument. Detection of tetracycline between 0.1 μg/mL - 1 μg/mL was achieved, with a readout time (including sample treatment) presented in 20 min. The magneto-immunoassay described provides a rapid, low-cost, de-skilled and analytically-sensitive solution for tetracycline screening at the point-of-sampling, with potential applications for other prevalent antibiotic families used in the international farming and food industry.</p></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"41 ","pages":"Article 100566"},"PeriodicalIF":5.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1883595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photonic crystal fibre for blood components sensing","authors":"Abdul Mu'iz Maidi ,&nbsp;Md. Abul Kalam ,&nbsp;Feroza Begum","doi":"10.1016/j.sbsr.2023.100565","DOIUrl":"https://doi.org/10.1016/j.sbsr.2023.100565","url":null,"abstract":"<div><p>A photonic crystal fibre (PCF)-based sensor has been proposed and thoroughly investigated for the identification of blood components, including red blood cells, haemoglobin, white blood cells, plasma, and water. To evaluate the sensor's sensing and propagation properties, a numerical analysis was performed using the COMSOL Multiphysics software. The proposed sensor design features an octagonal core and two layers of cladding with octagonal and circular air holes. At the optimal wavelength of 7.0 μm, the extensive simulation results confirms that the proposed sensor achieves high relative sensitivity of 99.89%, 99.13%, 97.95%, 97.77%, and 96.68% for red blood cells, haemoglobin, white blood cells, plasma, and water, respectively. Furthermore, the design demonstrates favourable confinement loss, propagation constant, V-parameter, spot size, and beam divergence. Therefore, the proposed PCF-based sensor holds great promise not only for medical sensing applications but also for optical communications. Its advanced design and highly sensitive capabilities make it a valuable tool for a wide range of potential applications in the biomedical and telecommunications fields.</p></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"41 ","pages":"Article 100565"},"PeriodicalIF":5.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3266018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasticizer-free Hydrazonic Dibenzo-18-crown-6 derived lead ion sensing electrodes","authors":"Deneikah T. Jackson ,&nbsp;Peter N. Nelson ,&nbsp;Kimberly Weston ,&nbsp;Richard A. Taylor","doi":"10.1016/j.sbsr.2023.100570","DOIUrl":"https://doi.org/10.1016/j.sbsr.2023.100570","url":null,"abstract":"<div><p>The redox behaviours of three dibenzo-18-crown-6 hydrazones were investigated via cyclic and square wave voltammetries, prior to their electropolymerization onto a platinum working electrode to yield three novel Ion Selective Electrodes (ISEs) whose lead sensing behaviours were investigated via Differential Pulse Anodic Stripping Voltammetry (DPASV) and Electrochemical Impedance Spectroscopy (EIS), subsequent to their characterization via Scanning Electron Microscopy (SEM). For all three ISEs, the electrosynthesized polymeric Ion Selective Membranes (ISM) are multi-layered with pores of diverse dimensions; a feature which allows wide linear ranges (4–107 ppm) in all cases, relative to Flame Atomic Absorption Spectroscopy (FAAS); one of the traditional “go-to” methods for metal ion quantification. Additionally, these ISEs allow selective detection of Pb²⁺ at concentrations below 20 ppm even in the presence of high concentrations of competing metal ions, except when excess Al³⁺ is present. Overall, given the relative ease of modification and low cost of these hydrazonic dibenzo-18-crown-6 ISEs, coupled with their performance, they represent a viable starting point for the development of high quality, low cost lead ISEs.</p></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"41 ","pages":"Article 100570"},"PeriodicalIF":5.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1694000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An electrochemical immunosensor on a carbon nanofiber/gold nanoparticles platform for the detection of alpha-feto protein cancer biomarker","authors":"Foluke O.G. Olorundare ,&nbsp;Dimpo S. Sipuka ,&nbsp;Tsholofelo I. Sebokolodi ,&nbsp;Sesethu Makaluza ,&nbsp;Nyasha Midzi ,&nbsp;Tetsuya Kodama ,&nbsp;Omotayo A. Arotiba ,&nbsp;Duduzile Nkosi","doi":"10.1016/j.sbsr.2023.100574","DOIUrl":"10.1016/j.sbsr.2023.100574","url":null,"abstract":"<div><p>We report an alpha – fetoprotein (AFP) cancer biomarker electrochemical immunosensor detected on a carbon nanofiber (CNF) and gold nanoparticles (AuNPs) platform. Functionalised carbon nanofiber and gold nanoparticles were immobilised through drop drying and electrodeposition techniques respectively on a glassy carbon electrode (GCE). AFP antibody was immobilised on the platform overnight followed by blocking of nonspecific binding sites with bovine serum albumin (BSA) for 4 h. The AFP antigen was incubated on the platform for 40 min at 35 °C for the antigen – antibody interaction. CNF and AuNPs were characterised by electron microscopy and Fourier transform infrared (FTIR) spectroscopy. The modified electrodes and the immunosensor were characterised by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. The electrochemical response of the GCE/CNF/AuNPs electrode and the resulting immunosensor was significantly enhanced by the nanocomposite of CNF and AuNPs. The electrochemical immunosensing measurement for AFP was carried out under optimal conditions at a concentration range from 0.005 to 500 ng mL⁻¹ in 5 mM ferri/ferrocyanide solution. A low limit of detection of 0.50 pg mL⁻¹ from square wave voltammetry (SWV) and 0.48 pg mL⁻¹ from EIS measurements were obtained. The immunosensor exhibited good sensitivity, good selectivity, good repeatability, and was stable at 4 °C over a period of three weeks.</p></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"41 ","pages":"Article 100574"},"PeriodicalIF":5.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214180423000260/pdfft?md5=41880337af2b21f254b3bbf45b72bf68&pid=1-s2.0-S2214180423000260-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42735866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advanced carbon dots derived natural products and aptasensor-based carbon dots for detection of pesticides","authors":"Sriati Wahyudi ,&nbsp;Ayi Bahtiar ,&nbsp;Camellia Panatarani ,&nbsp;Anas ,&nbsp;Risdiana","doi":"10.1016/j.sbsr.2023.100576","DOIUrl":"10.1016/j.sbsr.2023.100576","url":null,"abstract":"<div><p>Carbon dots (CDs) have gained a lot of attention and have been investigated extensively over the last decade due to their exceptional physical and chemical qualities including water solubility, low toxicity, biocompatibility, tiny size, fluorescence, and simplicity of modification. The employment of more ecologically friendly technologies to prepare high-quality CDs remains a challenge. The use of renewable, low-cost, and green biomass resources is not only required for large-scale synthesis of natural product CDs but also supports the development of sustainable applications, particularly in pesticide detection. Pesticide detection is critical because pesticides are commonly used in agriculture and the environment, potentially harming human health and the ecosystem. In this review, we discuss the most recent breakthroughs in natural product CDs, such as the detection of pesticides from various carbon sources using the applied sensing method. High sensitivity and selectivity pesticide sensors are critically needed to control food safety, protect ecosystems, and prevent disease. Therefore, we also present an overview of current breakthroughs and new trends in optical sensors for pesticide detection based on fluorescence and electrochemiluminescence (ECL) paired with pesticide-specific aptamer recognition element types based on CDs.</p></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"41 ","pages":"Article 100576"},"PeriodicalIF":5.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214180423000284/pdfft?md5=1a755071516654094b9cae9fb57c3ac9&pid=1-s2.0-S2214180423000284-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44595058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanosensors and their applications in early diagnosis of cancer","authors":"Masoud Khazaei ,&nbsp;Marzieh Sadat Hosseini ,&nbsp;Ali Moshfegh Haghighi ,&nbsp;Majid Misaghi","doi":"10.1016/j.sbsr.2023.100569","DOIUrl":"https://doi.org/10.1016/j.sbsr.2023.100569","url":null,"abstract":"<div><p>According to WHO and other key statistics, cancer is a leading cause of death worldwide, accounting for nearly 10 million deaths in 2020. By identifying cancer early, it is more likely to respond to treatment, resulting in a better chance of survival as well as a lower cost of treatment. Early detection of cancer and avoiding delays in treatment can significantly improve the lives of cancer patients. Nanomaterial-based sensors with the ability to extract and identify tumor-specific biomarkers, circulating tumor cells, or extracellular vesicles secreted by the tumor have the potential to diagnose cancer considerably earlier and enhance patient long-term survival. In this review, we provide the application of nanosensors for the early detection of cancer and the recent techniques for clinically diagnosing cancer patients' biomarker levels. Furthermore, the presented works are compared based on their sensitivity and selectivity in the detection of different kinds of cancer biomarkers.</p></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"41 ","pages":"Article 100569"},"PeriodicalIF":5.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1883596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-physics simulations and experimental comparisons for the optical and electrical forces acting on a silica nanoparticle trapped by a double-nanohole plasmonic nanopore sensor","authors":"Homayoun Asadzadeh ,&nbsp;Scott Renkes ,&nbsp;MinJun Kim ,&nbsp;George Alexandrakis","doi":"10.1016/j.sbsr.2023.100581","DOIUrl":"10.1016/j.sbsr.2023.100581","url":null,"abstract":"<div><p>Bimodal optical-electrical data generated when a 20 nm diameter silica (SiO₂) nanoparticle was trapped by a plasmonic nanopore sensor were simulated using Multiphysics COMSOL and compared with sensor measurements for closely matching experimental parameters. The nanosensor, employed self-induced back action (SIBA) to optically trap nanoparticles in the center of a double nanohole (DNH) structure on top a solid-state nanopores (ssNP). This SIBA actuated nanopore electrophoresis (SANE) sensor enables simultaneous capture of optical and electrical data generated by several underlying forces acting on the trapped SiO₂ nanoparticle: plasmonic optical trapping, electroosmosis, electrophoresis, viscous drag, and heat conduction forces. The Multiphysics simulations enabled dissecting the relative contributions of those forces acting on the nanoparticle as a function of its location above and through the sensor's ssNP. Comparisons between simulations and experiments demonstrated qualitative similarities in the optical and electrical time-series data generated as the nanoparticle entered and exited from the SANE sensor. These experimental parameter-matched simulations indicated that the competition between optical and electrical forces shifted the trapping equilibrium position close to the top opening of the ssNP, relative to the optical trapping force maximum that was located several nm above. The experimentally estimated minimum for the optical force needed to trap a SiO₂ nanoparticle was consistent with corresponding simulation predictions of optical-electrical force balance. The comparison of Multiphysics simulations with experiments improves our understanding of the interplay between optical and electrical forces as a function of nanoparticle position across this plasmonic nanopore sensor.</p></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"41 ","pages":"Article 100581"},"PeriodicalIF":5.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214180423000338/pdfft?md5=d37f35adcb2856078ff07b2c2646bfda&pid=1-s2.0-S2214180423000338-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43024601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}