Dual-target electrochemical aptasensor for the simultaneous detection of interleukin-6 and tumor necrosis factor-α in biological fluids

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research Pub Date : 2025-08-01 DOI:10.1016/j.sbsr.2025.100866

Maria-Bianca Irimes , Alexandra Pusta , Daniel Coker , Paul Cristian Martian , Maria Suciu , Stanca-Lucia Pandrea , Mihaela Tertis , Cecilia Cristea , Radu Oprean

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Abstract

Cytokines are key signaling biomolecules involved in cell growth, immune regulation, inflammation, and cancer-related processes, being valuable biomarkers for diagnosing medical conditions, assessing prognosis, and monitoring treatment efficacy. This study aimed to develop a customized platform enabling the simultaneous electrochemical detection of Interleukin-6 (IL-6) and Tumor Necrosis Factor-α (TNF-α) in biological fluids.

The platform was fabricated using in-lab printed electrochemical cells. The working electrode was functionalized with Au and Pt nanoparticles to enhance detection sensitivity. The specificity towards the targets was achieved by immobilizing two distinct aptamers, each labeled with a different redox probe, allowing parallel signal readouts and implicitly the simultaneous detection of the two biomarkers. The aptasensor demonstrated simultaneous detection of IL-6 and TNF-α within a linear range of 5–5000 pg/mL, achieving a limit of detection of 1.6 pg/mL. The dual-target aptasensor was validated using real biological samples, including raw saliva and sweat, collected from patients and healthy individuals. Results obtained from the aptasensor were cross-verified with ELISA and validated through statistical analysis.

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同时检测生物体液中白细胞介素-6和肿瘤坏死因子-α的双靶电化学感应传感器

细胞因子是参与细胞生长、免疫调节、炎症和癌症相关过程的关键信号生物分子，是诊断疾病、评估预后和监测治疗效果的有价值的生物标志物。本研究旨在开发一种定制的平台，能够同时电化学检测生物体液中的白细胞介素-6 （IL-6）和肿瘤坏死因子-α (TNF-α)。该平台是用实验室打印的电化学电池制造的。工作电极被金和铂纳米粒子功能化，以提高检测灵敏度。对目标的特异性是通过固定两个不同的适体来实现的，每个适体都用不同的氧化还原探针标记，允许并行信号读出，并隐含地同时检测两种生物标志物。该适体传感器在5-5000 pg/mL的线性范围内同时检测IL-6和TNF-α，达到1.6 pg/mL的检测限。双靶点适配体传感器使用真实的生物样本进行验证，包括从患者和健康个体收集的原始唾液和汗液。与酶联免疫吸附试验（ELISA）进行交叉验证，并进行统计分析。

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来源期刊

Sensing and Bio-Sensing Research Engineering-Electrical and Electronic Engineering

CiteScore

10.70

自引率

3.80%

发文量

审稿时长

87 days

期刊介绍： Sensing and Bio-Sensing Research is an open access journal dedicated to the research, design, development, and application of bio-sensing and sensing technologies. The editors will accept research papers, reviews, field trials, and validation studies that are of significant relevance. These submissions should describe new concepts, enhance understanding of the field, or offer insights into the practical application, manufacturing, and commercialization of bio-sensing and sensing technologies. The journal covers a wide range of topics, including sensing principles and mechanisms, new materials development for transducers and recognition components, fabrication technology, and various types of sensors such as optical, electrochemical, mass-sensitive, gas, biosensors, and more. It also includes environmental, process control, and biomedical applications, signal processing, chemometrics, optoelectronic, mechanical, thermal, and magnetic sensors, as well as interface electronics. Additionally, it covers sensor systems and applications, µTAS (Micro Total Analysis Systems), development of solid-state devices for transducing physical signals, and analytical devices incorporating biological materials.