Sensing and Bio-Sensing Research最新文献

{"title":"Miniaturized multispectral imaging for microfluidic pH sensing","authors":"Yushan Meng ,&nbsp;Dechuan Sun ,&nbsp;Bryce Widdicombe ,&nbsp;Dina Thankom Jacob ,&nbsp;Yiling Yang ,&nbsp;Xumei Gao ,&nbsp;Alastair G. Stewart ,&nbsp;Ranjith Rajasekharan Unnithan","doi":"10.1016/j.sbsr.2025.100764","DOIUrl":"10.1016/j.sbsr.2025.100764","url":null,"abstract":"<div><div>Multispectral lab-on-chip imaging based on complementary metal-oxide semiconductor (CMOS) is an innovative technology that supports portable microfluidic platforms and enables the extraction of spectral information from specimens under multiple wavelengths. Accurate pH sensing is essential across a diverse range of chemical, biological, medical, and environmental applications. In this paper, we presented a new approach for pH sensing using the CMOS-based multispectral lab-on-chip imaging. Using a six-band color filter array that spans the optical spectrum in the visible and near-infrared range and a U-shape PDMS microfluidic channel, images of solutions with specific pH values were captured by a monochrome CMOS image sensor. To predict pH values, we developed a Support Vector Regression (SVR) model, which was trained using captured images of the six bands. Predicted pH values were obtained by the model with an RMSE of 0.364. Our research demonstrates that multispectral lab-on-chip imaging represents a miniature and rapid method to achieve pH sensing with high sensitivity and accuracy.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100764"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DPV and EIS-based ultrasensitive aptasensor for VEGF165 detection based on nanoporous gold platform modified with SH-aptamer","authors":"Bahareh Pourbahram ,&nbsp;Samira Mansouri Majd ,&nbsp;Mojtaba Shamsipur","doi":"10.1016/j.sbsr.2025.100766","DOIUrl":"10.1016/j.sbsr.2025.100766","url":null,"abstract":"<div><div>The sensitive detection of trace amounts of vascular endothelial growth factor (VEGF₁₆₅) in samples holds significant potential for clinical cancer diagnosis across various cancer types. This work introduces a novel and cost-effective dual strategy for developing an ultrasensitive electrochemical aptasensor based on the self-assembly of SH-aptamer onto a nanoporous gold electrode for VEGF₁₆₅ detection. SEM and electrochemical characterization confirmed the formation of the nanoporous structure and the increase of the surface area of the gold electrode from 0.1199 cm² to 0.1875 cm². The sensing method relied on the interaction between the DNA probe and VEGF₁₆₅, which increased the charge transfer resistance of Fe(CN)₆^3−/4- in the (signal-on) strategy, as determined by EIS. Conversely, it diminished the reduction peak current of the methylene blue probe in the (signal-off) strategy, as assessed by Differential Pulse Voltammetry (DPV). The limit of detection and linear dynamic range of the prepared electrode were found to be 0.25 pM and 2.5 to 140 pM, respectively, using DPV, and 0.18 pM and 5 to 200 pM, respectively, using EIS. The aptasensor demonstrated desirable stability after storing the aptasensor in a buffer solution for one week. Furthermore, analogous proteins did not exhibit significant interference. Also, detecting the VEGF₁₆₅ cancer marker in undiluted human serum samples had excellent recovery with relatively low standard deviation percentages. Therefore, the findings indicate that the proposed aptasensor could potentially be utilized in the future design of a non-invasive cancer diagnostic kit using a blood or interstitial fluid test instead of the painful and aggressive biopsy method.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100766"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143480000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A wireless instrumented insole for monitoring plantar pressure during human locomotion","authors":"Mariana Alegria ,&nbsp;Mariana Ballesteros ,&nbsp;David Cruz-Ortiz","doi":"10.1016/j.sbsr.2025.100752","DOIUrl":"10.1016/j.sbsr.2025.100752","url":null,"abstract":"<div><div>This work proposes a wireless instrumented insole for monitoring plantar pressure (PP) during human locomotion. The insole is composed of two elements. The first is a mold manufactured with thermoplastic polyurethane using tridimensional printing techniques. The second one is a soft cover fabricated with a liquid polymer Ecoflex™ 00–30 from Smooth-On. The insole considers a microcontroller Arduino Nano and 24 force-sensing resistors located in ten anatomic regions of the foot with a sampling frequency of 70 Hz. The device sends the data through wireless communication based on a Bluetooth module to a graphical user interface in a computer where the data is received, displayed, and stored. A test was carried out with ten healthy volunteers (five men and five women) to validate the PP measured with the device. The data obtained were analyzed and compared with the literature, considering relevant characteristics in the PP analysis.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100752"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143353638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simplified optical monitoring systems for detecting leukocyte-derived hypochlorite ions using small amounts of whole blood","authors":"Kozo Takeuchi ,&nbsp;Toshiyuki Miwa ,&nbsp;Takayuki Sato ,&nbsp;Takahiro Shikayama ,&nbsp;Hiroshi Tsuchiya ,&nbsp;Kimiko Kazumura","doi":"10.1016/j.sbsr.2024.100722","DOIUrl":"10.1016/j.sbsr.2024.100722","url":null,"abstract":"<div><div>Here, we developed two simplified optical systems, FLP-H3200 and FLP-H4200, designed to detect leukocyte-derived hypochlorite ions via fluorescence (FL-OCl⁻) using only three microliters of whole blood. First, the new systems employed a lower-cost photodiode as the photodetector instead of a photomultiplier tube in their preceding system CFL-H2200. The systems featured a simplified optical configuration optimized for the blood measurement fluidic chip, enabling efficient focusing of the desired FL-OCl⁻ signal and obtaining a signal intensity comparable to that obtained with CFL-H2200. These improvements made the new systems more compact, lightweight, and cost-effective than CFL-H2200. Additionally, in FLP-H4200, the intensity of the excitation light source, the light-emitting diode, was set higher than that in FLP-H3200, further enhancing the FL-OCl⁻ signal. Using the fluorescent reagent fluorescein, we confirmed that the fluorescence sensitivity of the new systems was comparable to that of CFL-H2200. Finally, blood analysis data using FLP-H3200 and FLP-H4200 showed a high correlation. This study broadens the scope of clinical examinations by providing accessible and efficient tools for monitoring leukocyte activity using whole blood.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100722"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A data ensemble-based approach for detecting vocal disorders using replicated acoustic biomarkers from electroglottography","authors":"Lizbeth Naranjo ,&nbsp;Carlos J. Pérez ,&nbsp;Daniel F. Merino","doi":"10.1016/j.sbsr.2025.100741","DOIUrl":"10.1016/j.sbsr.2025.100741","url":null,"abstract":"<div><h3>Introduction</h3><div>The relevant prevalence of voice-related pathologies underscores the need for robust computer-aided diagnostic (CAD) systems capable of supporting early detection and continuous monitoring. Electroglottography (EGG), a non-invasive technique measuring vocal fold contact area, has proven valuable in identifying and diagnosing vocal disorders.</div></div><div><h3>Problem statement</h3><div>Traditional diagnostic methods struggle with the dependent nature of EGG measurements within subjects, leading to challenges in managing within-subject variability and supporting multi-class classification.</div></div><div><h3>Objectives</h3><div>This study aims to design, implement, and evaluate two ensemble-based approaches that address the dependency in EGG measurements. The goal is to enhance the detection of vocal disorders by managing within-subject variability and facilitating multi-class classification.</div></div><div><h3>Methods</h3><div>The proposed methods utilize replicated acoustic biomarkers derived from EGG signals. Simulation-based experiments were conducted to assess the robustness and effectiveness of these methods. Additionally, experiments were performed using EGG signals from the Saarbrüecken Voice Database (SVD).</div></div><div><h3>Results</h3><div>Simulation results indicate that integrating replicated data improves accuracy rates compared to non-replicated models. Experiments on SVD demonstrated the robustness of the proposed methodology across different vowels in classifying healthy individuals, patients with laryngitis, and those with vocal fold paralysis.</div></div><div><h3>Conclusion</h3><div>The data ensemble-based approaches developed effectively manage the dependent nature of EGG measurements, enhancing the detection and classification of vocal disorders. These methods can be applied to other data types where replications play a key role. Future research should focus on collecting comprehensive EGG databases and further exploring multi-class classification methods to solidify EGG and machine learning as a valuable tool for non-invasive assessment of laryngeal function.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100741"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of petrochemicals using photonic crystal fiber (PCF) in terahertz domain","authors":"Md. Anowar Kabir ,&nbsp;Md. Selim Hossain ,&nbsp;Ashik Muhammed Arman ,&nbsp;Md. Al-Amin ,&nbsp;Shuvo Sen","doi":"10.1016/j.sbsr.2025.100737","DOIUrl":"10.1016/j.sbsr.2025.100737","url":null,"abstract":"<div><div>A photonic crystal fiber (PCF) with a hexahedron core has been introduced for usage in petrochemical sensing operations. The performance of the proposed sensor has been statistically analyzed in the frequency band of 1.0 to 3.0 THz. Pure petrol, kerosene, and diesel are poured into the hexahedron core hole. The Finite element Method (FEM) is handled for the simulation and precise calculation and investigation. The sensor utilizing PCF exhibits a relative sensitivity of about 97.80 %, 97.45 %, and 96.25 % and a confinement loss of approximately 1.88 × 10⁻⁸ dB/m, 1.90 × 10⁻⁸ dB/m, and 1.85 × 10⁻⁸ dB/m at 2.20 THz for all investigated Petrol, Kerosene, and Diesel petrochemicals. Moreover, effective areas are values of 7.75 × 10⁻⁸ m², 7.80 × 10⁻⁸ m², and 6.98 × 10⁻⁸ m², and the effective material loss for Petrol, Kerosene, and Diesel of 0.0066168 cm⁻¹, 0.0066955 cm⁻¹ and 0.0066988 cm⁻¹, respectively at 2.20 THz. Both 3D printing and extrusion can be used to create this photonic crystal fiber (PCF) sensor. In addition, this heptagonal photonic crystal fiber sensor will be used for biomedical sensing and various industry fields related to sensing sectors.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100737"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface acoustic waves (SAW) sensor for the active detection of Microcystin-LR (Cyanobacteria)","authors":"Debdyuti Mandal ,&nbsp;Tally Bovender ,&nbsp;Robert D. Geil ,&nbsp;Debabrata Sahoo ,&nbsp;Sourav Banerjee","doi":"10.1016/j.sbsr.2024.100724","DOIUrl":"10.1016/j.sbsr.2024.100724","url":null,"abstract":"<div><div>Cyanobacteria are a family of prokaryotic bacteria whose death causes the release of harmful toxins. Upon ingestion, these toxins produce symptoms similar to food poisoning and are dangerous to humans, as well as livestock. As there is no easily accessible way to remove cyanotoxins from water sources, thus detection before consumption is vitally important. In this article, we report a shear horizontal surface acoustic wave (SH-SAW) based sensor for the active detection of the microcystin congener, microcystin-LR (MC-LR). The sensing platform was devised on 36° YX cut-LiTaO₃ which is a piezoelectric substrate. The sensor system was designed based on delay line configuration and was actively coated with silicon dioxide as a waveguide layer for better mass-load sensitivity. Unlike conventional SAW, the sensing platform utilizes a 5-count tone burst signal, enhancing sensitivity due to its sensitive coda waves. Signal transformation and analysis were made for distinct detection in the frequency domain. The sensor also incorporates the functionalization of gold nanospheres for a high surface-to-volume ratio and enhanced degree of orientation leading to better sensitivity. The sensor detection limit was down to 5.13 nM. Further evidence was provided by selectivity analysis and the sensor could identify MC-LR from the other biomarkers.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100724"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ternary metal oxide of CuNiCo2O4nanorods (1D) distributed on g-C3N4 (2D) nanocomposite for non-enzymatic glucose sensing application","authors":"R. Thenmozhi,&nbsp;R. Navamathavan","doi":"10.1016/j.sbsr.2025.100757","DOIUrl":"10.1016/j.sbsr.2025.100757","url":null,"abstract":"<div><div>Non-enzymatic glucose sensing has a major demand in research community for developing a good glucose sensor. Because enzyme based glucose sensor has several disadvantages such as high cost, difficult fabrication process and instability. To overcome these disadvantages, transition metal oxides (TMO) with g-C₃N₄ nanocomposite are a good choice for non-enzymatic medium for developing a good glucose sensor. Transition metal oxide has multiple oxidation state, different morphology, high conductivity, enhanced catalytic activity and 2D graphitic carbon nitride has a higher stability. In this work, our particular interest in ternary metal oxide (CuNiCo₂O₄) nanorods (1D) distributed on surface of the g-C₃N₄ (2D) nanocomposite were prepared by simple hydrothermal method. The prepared nanocomposite was performed a basic studies such as XRD, FESEM, HRTEM, FTIR, XPS and an electrochemical studies using nickel foam as a current collector. Chronoampermetry analysis produced a sensitivity of 4368 μA Cm⁻² mM⁻¹ and low detection limit (LOD) 1.91 μM. The produced response time is 4 s. The measured results of the CuNiCo₂O₄nanorods (1D) on the surface of g-C₃N₄ (2D) nanocomposite could be a satisfied material for non-enzymatic glucose sensor.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100757"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roles of nanotechnology in electrochemical sensors for medical diagnostic purposes: A review","authors":"Ali R. Jalalvand,&nbsp;Mohammad Mehdi Karami","doi":"10.1016/j.sbsr.2024.100733","DOIUrl":"10.1016/j.sbsr.2024.100733","url":null,"abstract":"<div><div>The rapid advancements in nanotechnology have significantly enhanced the capabilities of electrochemical sensors, particularly in the realm of medical diagnostics. This review article explores the integration of nanomaterials such as nanoparticles, nanowires, nanotubes, and graphene in electrochemical sensors and their transformative impact on disease detection and health monitoring. Nanotechnology-enhanced sensors offer remarkable improvements in sensitivity, specificity, miniaturization, and making them ideal for point-of-care testing and real-time analysis. This review article provides a comprehensive information about the types and mechanisms of electrochemical sensors, the unique properties of nanomaterials that enhance sensor performance, and the diverse medical applications ranging from biomarker detection to pathogen identification. Despite the promising benefits, several challenges including technical, regulatory, and market barriers are discussed alongside potential strategies to overcome these hurdles. The ongoing research and development in this field promise to revolutionize medical diagnostics by providing rapid, accurate, and accessible testing solutions ultimately improving patient outcomes and healthcare efficiency.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100733"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-pot green synthesis of BSA-capped O-CQDs as an effective fluorescent sensing platform for sensitive and selective detection of promethazine drug","authors":"S. Viji ,&nbsp;A. Dinesh ,&nbsp;K. Radhakrishnan ,&nbsp;L. Srimathi Priya ,&nbsp;C. Sivasankari ,&nbsp;Madhappan Santhamoorthy ,&nbsp;Manikandan Ayyar ,&nbsp;V. Mohanavel ,&nbsp;Mohamed Hashem ,&nbsp;Hassan Fouad ,&nbsp;G. Ramachandran ,&nbsp;S. Santhoshkumar","doi":"10.1016/j.sbsr.2025.100756","DOIUrl":"10.1016/j.sbsr.2025.100756","url":null,"abstract":"<div><div>Reliability in biosensing technologies is crucial for the accurate detection and measurement of target molecules, prompting strategies to minimise non-specific binding events that may result in inaccurate findings. This work reports on bovine serum albumin (BSA)-capped oxygen rich carbon quantum dots (O-CQDs) to improve the accuracy of promethazine sensing. The CQDs were produced utilizing a hydrothermal technique with apple juice as a sustainable carbon source. BSA-Caped O-CQDs improve their biocompatibility and stability in physiological conditions while also reducing non-specific interactions with other biomolecules. Several spectroscopic methods, such as fluorescence, X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR), were used to evaluate this enhanced specificity and sensitivity. Obtained results show that BSA-O-CQDs had higher specificity for promethazine Furthermore, their ability to resolve non-specific binding was assessed under simulated physiological backgrounds, demonstrating their promise for robust biosensing applications. This sensor method achieved a high selectivity with LOD of 0.2 μM from range of 10–90 μM indicating its suitability for precise promethazine measurement in environmental samples. The primary advantages of this technique include simplified sample preparation together with economic advantages which make it a critical instrument for public health monitoring as well as cutting-edge pharmaceutical research. Sustainable O-CQDs demonstrate the potential for creating environmentally friendly biosensors.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100756"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}