Iranian Journal of Pathology最新文献

{"title":"Bioinformatics Analysis of Peroxisomal Biogenesis Factor Proteins in Breast Malignancy for Introducing Potential Prognostic Biomarkers.","authors":"Nima Mahdei Nasirmahalleh, Mina Hemmati, Negin Parsamanesh","doi":"10.30699/ijp.2025.2030953.3311","DOIUrl":"10.30699/ijp.2025.2030953.3311","url":null,"abstract":"<p><strong>Background & objective: </strong>Breast cancer (BC) is the most common type of malignant neoplasm and is the primary cause of mortality among women aged 45 to 55 years. Studies indicate that cancer displays irregular metabolic patterns in contrast to normal tissue. Furthermore, there is compelling evidence supporting the significant role of peroxisomes in the intricate metabolic processes of cancer. Peroxisomal biogenesis factors (PEXs), which are peroxisomal proteins, control activities such as the degradation and biogenesis of peroxisomes. Hence, the correlation between peroxisomal biogenesis factor expression and BC was explored, to introduce key proteins and potential biomarkers by analyzing.</p><p><strong>Methods: </strong>This study utilized UALCAN, GenExMiner v4.8, Metascape, STRING, TIMER, the Kaplan-Meier plotter, The Human Protein Atlas, MirTarBase, and cBioportal.</p><p><strong>Results: </strong>The transcriptional levels of PEX6/7/10/11B/13/16 in BC tissues were significantly elevated, whereas the transcriptional levels of PEX2/3/5/11A/12/19 were significantly reduced. High expression levels of PEX 2/3/10/12/11G /26/13/16/14 were significantly related to shorter relapse-free survival, and higher mRNA expression of PEX 11B/11G/11A/12/19 was significantly associated with longer overall survival of BC patients. We identified has-miR-4318 and has-7106-3p as more correlated miRNAs with the PEX family.</p><p><strong>Conclusion: </strong>Our results may provide novel insights for the selection of therapeutic targets and prognostic biomarkers for BC.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"20 2","pages":"181-197"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12142011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyclooxygenase-2 Overexpression and its Association with Histopathological Features of Human Malignant Melanoma.","authors":"Nasrin Shayanfar, Zeinab Gholizade, Fatemeh Montazer, Kambiz Kamyab, Aram Nazari, Ali Zare Mirzaie","doi":"10.30699/ijp.2025.2042091.3357","DOIUrl":"10.30699/ijp.2025.2042091.3357","url":null,"abstract":"<p><strong>Background & objective: </strong>Melanoma is one of the most common types of skin cancer with an annually increasing mortality rate. Cyclooxygenase-2 (COX-2) plays an imperative role as a cancer biomarker in the biosynthesis of prostaglandin and thromboxane during inflammatory reactions. Its overexpression has been demonstrated in various cancer types, including melanoma. However, its clinicopathological association with melanoma is controversial. We aimed to immunohistochemically evaluate COX-2 expression in malignant melanoma (MM) tumors.</p><p><strong>Methods: </strong>In this cross-sectional retrospective study, blocks from patients with MM who were referred to Rasool-Akram and Razi hospitals between 2011 and 2020 were collected and immunohistochemically evaluated using COX-2 antibody. The intensity and percentage of COX-2 expression was determined in tumoral tissues, and its association with clinical and histological features of patients were evaluated.</p><p><strong>Results: </strong>A total of 39 patients diagnosed with MM were included in this study, of whom 20 (51.3%) were male and 19 (48.7%) were female, with an average age of 57.28 ± 14.37 (range 14-87 years). The most common histological subtypes were acral melanoma (30.8%) and nodular melanoma (20.5%). The most common locations of tumor involvement were the lower (33.3%) and upper limbs (23.1%). Ulcers and vascular-lymphatic invasion were observed in 33.3% and 5.1% of cases, respectively. 38.5% of tumors were in level IV according to Clark's level. Elastosis was present in 13% of samples. Approximately 87% of MM samples showed COX-2 expression, 61.5% of which were strong. There was a significant association between COX-2 expression and tumor location (P = .04).</p><p><strong>Conclusion: </strong>\u0000 <b>Our findings highlight that the COX-2 protein is considerably expressed in MM tumors. Therefore, therapeutic strategies with the aim of targeting COX-2 might be considered in the prevention or treatment of MM. However, it remains to be explored whether COX-2 might be a prognostic marker of MM.</b>\u0000 </p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"20 2","pages":"207-216"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12142022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of The Milan System for Reporting Salivary Gland Cytology: A 5-Year Retrospective Review.","authors":"Afrooz Arashloo, Hana Saffar, Maryam Lotfi, Fereshteh Ameli","doi":"10.30699/ijp.2024.2021304.3249","DOIUrl":"10.30699/ijp.2024.2021304.3249","url":null,"abstract":"<p><strong>Background & objective: </strong>Use of fine needle aspiration cytology (FNAC) in the diagnosis of salivary gland neoplasms is controversial due to the diverse morphologic patterns and overlapping features between benign and malignant lesions. The Milan system has been introduced to report salivary gland cytopathology. The present study aimed to reclassify salivary gland lesions according to the Milan system for reporting salivary gland cytopathology (MSRSGC) to determine the risk of malignancy (ROM) and to estimate the diagnostic accuracy of the Milan system.</p><p><strong>Methods: </strong>In this retrospective cohort study, 136 salivary gland fine needle aspiration cytology samples taken from patients referred to Imam Khomeini and Amir-Aalam Hospital, Tehran, Iran, from 2016 to 2021, were retrieved along with the histopathological follow-up. Cytology smears were reviewed and reclassified based on MSRSGC. In addition, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and diagnostic accuracy were calculated.</p><p><strong>Results: </strong>ROM for each category was 26.7% for non-diagnostic, 12.5% for non-neoplastic, 40% for atypia of undetermined significance (AUS), 0 for benign neoplasm, 0 for salivary gland neoplasm with uncertain malignant potential (SUMP), 100% for suspicious for malignancy, and 100% for malignant group. Sensitivity, specificity, PPV, and NPV in differentiating benign from malignant neoplasms based on MSRSGC were 75.9%, 100%, 100%, and 93.8%, respectively. Diagnostic accuracy was calculated as 94.8%.</p><p><strong>Conclusion: </strong>MSRSGC may be associated with a high accuracy in differentiation of benign from malignant salivary gland neoplasms, indicating its potential value as an effective classification system for reporting salivary gland cytology. The ROM for cytological categories except SUMP can be almost similar to that suggested by MSRSGC.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"20 1","pages":"76-83"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11887628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Diagnostic Accuracy of Impression Cytology for Conjunctival Lesions: A Comparative Study with Histopathology.","authors":"Fatemeh Eslami, Hamidreza Ghasemibasir, Sara Alipour, Ramin Mansouri","doi":"10.30699/ijp.2024.2025786.3284","DOIUrl":"10.30699/ijp.2024.2025786.3284","url":null,"abstract":"<p><strong>Background & objective: </strong>Conjunctival lesions have a wide range of histological manifestations that are difficult to distinguish clinically. The gold standard for diagnosis of these lesions is the pathological examination, a costly and invasive procedure that may also adversely affect patients. Therefore, clinical researchers seek less invasive, inexpensive, and easier methods to detect conjunctival lesions. This study aims to compare the accuracy of impression cytology with pathology in patients referred to Farshchian Hospital in Iran.</p><p><strong>Methods: </strong>In this descriptive/cross-sectional study, 64 patients with conjunctival lesions were selected from patients referred to Farshchian Hospital in Hamedan in 2021. A cytology specimen was obtained from the patients and sent to the laboratory. The diagnostic accuracy of this method was compared with pathological results (gold standard). Data were analyzed by SPSS 16 software.</p><p><strong>Results: </strong>The mean age of patients was 54.47 ±16.94 years; 70.3% were male, and 29.7% were female. In the cytologic and pathologic examination, 28.1% of the specimens showed dysplasia, and 71.9% were non-dysplastic. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the cytologic impression compared to the pathologic methods were 91.30%, 77.78%, 91.30%, 77.78%, and 87.5%, respectively. A positive and significant correlation was observed between pathological and cytological diagnosis scores (r=0.825, P-value<0.001).</p><p><strong>Conclusion: </strong>In conjunctival lesions, impression cytology may be a relatively accurate and sensitive procedure that can distinguish dysplastic from non-dysplastic conjunctival lesions.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"20 1","pages":"49-57"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11887644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IL-8/CD181 Mediated Inflammation in SLE-Associated Hemolytic Anemia.","authors":"Muslim Idan Mohsin, Samer A Mh Al-Hilali, Rusul Idan Mohsin, Mohammed Mohasin, Mohammed Jasim Mohammed Al-Shamarti","doi":"10.30699/ijp.2025.2051460.3403","DOIUrl":"10.30699/ijp.2025.2051460.3403","url":null,"abstract":"<p><strong>Background & objective: </strong>Systemic Lupus Erythematosus (SLE) is an autoimmune disease characterized by immune dysregulation, autoantibody production, and organ damage, notably in the kidneys. Cytokine imbalances contribute to SLE's diverse clinical presentations. This study investigated the roles of interleukin-8 (IL-8) and its receptor, CD181 (CXCR1), in SLE pathogenesis, specifically focusing on their association with hemolytic anemia, a severe complication.</p><p><strong>Methods: </strong>This research investigates the role of interleukin-8 (IL-8) and its cognate receptor, CXCR1 (CD181). It was analyzed clinical and demographic data from 250 SLE patients and quantified IL-8 and CD181 mRNA and protein expression in samples from patients with active SLE, inactive SLE, and SLE complicated by hemolytic anemia, comparing them to healthy controls.</p><p><strong>Results: </strong>Of the 250 samples, 84% were from SLE patients, with 67% in the active disease phase. Significant upregulation of both IL-8 and CD181 mRNA and protein was observed in SLE patients compared to controls. Specifically, mRNA expression was significantly elevated in active SLE (p=0.0001) and inactive SLE (p=0.01). Notably, IL-8 mRNA expression was significantly higher in SLE patients with hemolytic anemia (p<0.0001) compared to those without (p<0.01(.</p><p><strong>Conclusion: </strong>These findings suggest that the IL-8/CD181 axis plays a crucial role in the inflammatory processes and tissue damage associated with SLE, particularly in the development of hemolytic anemia.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"20 2","pages":"217-224"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12142021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commentary on \"p53 IHC Result as a Prognostic Tool in MDS\".","authors":"William Gesztes, Vahid Mehrtash, Victor E Nava","doi":"10.30699/ijp.2024.2027620.3295","DOIUrl":"10.30699/ijp.2024.2027620.3295","url":null,"abstract":"","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"20 1","pages":"140-141"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11887643/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Association Between RACK1 Gene Polymorphisms rs1279736 and rs3756585 in Uterine Cervical Cancer with the Risk Factor of HPV: A Focus on Squamous Cell Carcinoma (SCC).","authors":"Maryam Fekri Soofi Abadi, Fatemeh Hashemi, Saeedeh Shojaeepour, Sahar Amirpour-Rostami, Mohammad Reza Zangouey, Sara Pourshaikhali, Fatemeh Pouya, Shahriar Dabiri","doi":"10.30699/ijp.2024.2019194.3236","DOIUrl":"10.30699/ijp.2024.2019194.3236","url":null,"abstract":"<p><strong>Background & objective: </strong>Cervical cancer is the second most common malignancy among women worldwide. The Receptor for Activated C Kinase 1 (RACK1) has a key role in regulating the pathways of cell signaling, angiogenesis, cell migration, invasion, and metastasis. This study investigated the association of polymorphisms rs1279736 and rs3756585 with cervical cancer.</p><p><strong>Methods: </strong>In this study, 100 LB pap-smear and paraffin block samples of cervical squamous carcinoma associated with Human <i>papillomavirus (HPV)</i> were selected along with 100 LB smear cytology samples from healthy women with normal pap smears, serving as the control group. Genomic DNA was extracted from the samples, and the polymorphisms rs1279736 and rs3756585 in the RACK1 genes were examined using the RFLP-PCR and ARMS-PCR methods, respectively<b>.</b></p><p><strong>Results: </strong>The rs1279736 polymorphism shows that the chance of cervical cancer in individuals with CA and AA genotypes is 3.772 and 1.852 times that of CC genotypes, respectively. This suggests that the A allele is involved in developing cervical cancer (<i>P</i>=0.005; OR=2.113). The rs3756585 polymorphism indicates that the chance of cervical cancer in TG and GG genotypes is 0.373 and 4.235 times that of TT genotypes, respectively. This also points towards the involvement of the G allele in the development of cervical cancer (<i>P</i>=0.032; OR=1.800). Also, there was no significant relationship between the genotype of polymorphisms, age, and subtype of papillomavirus 16 and 18 in patients.</p><p><strong>Conclusion: </strong>The present study was conducted for the first time. Our results showed that two polymorphisms are significantly associated with cervical cancer.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"20 1","pages":"24-32"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11887637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vacuolating Cytotoxin A (VacA) and Extracellular Vesicles in <i>Helicobacter pylori</i>: Two Key Arms in Disease Development.","authors":"Seyedeh Faride Alavi Rostami, Mansoor Khaledi, Fatemeh Dalilian, Mahtab Mehboodi, Atefeh Akbari, Milad Shahini Shams Abadi, Pouria Khodaei Ataloo, Zeinab Mohsenipour, Samad Rastmanesh","doi":"10.30699/ijp.2024.2031417.3312","DOIUrl":"10.30699/ijp.2024.2031417.3312","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) are cell-derived vesicles that play a critical role in host-pathogen interactions, facilitating intercellular communication and transporting both pathogen- and host-derived molecules during infection spread. To regulate their environment, for instance, by modulating innate and adaptive inflammatory immune responses, pathogens may alter the composition of EVs produced by infected cells. Gastric cancer is one of the leading causes of cancer-related deaths worldwide, and Helicobacter pylori infection is considered a significant risk factor for its development. This cancer is characterized by significant inflammation mediated by EVs generated from infected host cells. H. pylori contributes substantially to inflammation, promoting disease progression. Moreover, H. pylori produces and releases vesicles known as outer membrane vesicles (H. pylori-OMVs), which contribute to the shrinkage and cellular transformation of the gastric epithelium. Although the vacuolating cytotoxin A (VacA) plays a critical role in pathogenesis, its association with EVs in H. pylori has not been previously addressed. Understanding the roles of extracellular vesicles and VacA during H. pylori infection-whether they benefit the host or the pathogen-could pave the way for new treatment approaches. This review briefly discusses the role of VacA and extracellular vesicles in the growth and pathogenesis of <i>H. pylori</i>.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"20 1","pages":"1-17"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11887638/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Diagnostic Methods in Patients with Squamous Intraepithelial Lesion in Women Infected with Multiple High-Risk Human Papillomaviruses.","authors":"Ensiyeh Bahadoran, Babak Rahmani, Esfandiyar Nazari, Aida Hosseinnezhad, Fatemeh Samieerad","doi":"10.30699/ijp.2024.2036312.3330","DOIUrl":"10.30699/ijp.2024.2036312.3330","url":null,"abstract":"<p><strong>Background & objective: </strong>Human papillomavirus (HPV) is a major cause of cervical cancer and mortality, particularly in low-income countries. Late diagnosis of cervical cancer often leads to advanced-stage disease, resulting in poorer prognosis and higher mortality, which underscores the critical need for effective early screening methods. Pap smears, colposcopy, and HPV testing are essential for early detection. This study addresses a gap in the literature by comparing the effectiveness of different diagnostic methods specifically in women with multiple high-risk HPV (hrHPV) infections-a population for which limited comparative data exist.</p><p><strong>Methods: </strong>This prospective cross-sectional study, approved by the Ethical Committee of Qazvin University (IR.QUMS.REC.1400.143), was conducted at Kowsar Hospital from 2022 to 2023 and included 608 patients infected with multiple hrHPV subtypes. Inclusion criteria required hrHPV confirmation by PCR genotyping and excluded patients without colposcopy and biopsy or with a history of cervical dysplasia. Participants underwent Pap smear cytology, colposcopy, and biopsy, with the biopsy specimens examined by a gynecologic pathologist. Statistical analyses included chi-square tests for qualitative variables and t-tests or ANOVA for quantitative variables, with a significance level set at P<0.05.</p><p><strong>Results: </strong>The average age of the patients was 38.54 years, with the majority aged 30-39 years (43.6%). Common symptoms included vaginal discharge (36.5%) and postcoital bleeding (34.9%). Pap smear results showed ASCUS in 43.6% of cases and LSIL in 39.1%. Colposcopy revealed acetowhite lesions in 45.6% and abnormal vascular patterns in 25.5%. Histopathology indicated that 72.4% of patients had CIN I. Smoking was significantly correlated with colposcopic findings (P=0.044). Colposcopy demonstrated the highest sensitivity (69.1%) for detecting cervical lesions, while cytology had the highest specificity (63.1%).</p><p><strong>Conclusion: </strong>Pap smear findings provide adequate diagnostic accuracy for hrHPV patients, but colposcopy offers superior sensitivity for detecting cervical lesions. Combining both methods is recommended to improve diagnosis.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"20 1","pages":"108-117"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11887635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Value of Cytology in Pancreatic Endoscopic Ultrasound-Guided Fine Needle Aspiration: Accuracy in Common Epithelial Pancreatobiliary Tumors and the Role of Cell Block Analysis.","authors":"Naser Rakhshani, Neda Soleimani, Sanaz Aghaei, Rasha Barakat, Ali Mohammad Keshtvarz Hesam Abadi","doi":"10.30699/ijp.2024.2036290.3329","DOIUrl":"10.30699/ijp.2024.2036290.3329","url":null,"abstract":"<p><strong>Background & objective: </strong>Limited literature describes the accuracy of endoscopic ultrasonography-fine needle aspiration (EUS-FNA) cytology in various types of pancreatic epithelial tumors, and this underscores the usefulness of cell blocks, and highlights potential diagnostic pitfalls.</p><p><strong>Methods: </strong>This study included 108 patients who underwent EUS-FNA pancreatobiliary cytology followed by surgery. Age, gender, tumor location, tumor size, presence or absence of a cell block, cytologic and pathologic diagnoses, and histologic tumor grade were recorded. Cytologic and pathologic slides were examined, and the cytologic accuracy was determined by comparing cytologic with the histopathologic results as the gold standard. Additionally, the impact of cell block on the cytologic accuracy was assessed.</p><p><strong>Results: </strong>EUS-FNA cytology showed an overall accuracy of 80%, a sensitivity of 90%, and a false-positive rate below 1%. Pancreatic ductal adenocarcinomas (PDAs) accounted for 65% of cases, followed by neuroendocrine tumors (NETs), solid pseudopapillary neoplasms (SPNs), mucinous cystic neoplasms (MCNs), and chronic pancreatitis. Diagnostic accuracy was higher for PDA and SPN than for NET and MCN and significantly improved to 100% in cases with a cell block.</p><p><strong>Conclusion: </strong>Combining pancreatobiliary cytology with a cell block significantly enhances diagnostic accuracy, reaching 100%. Moreover, poorly differentiated PDAs and well-differentiated organoid-type tumors, such as NETs and SPNs, demonstrate higher diagnostic accuracy.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"20 1","pages":"98-107"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11887639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}