The Association Between RACK1 Gene Polymorphisms rs1279736 and rs3756585 in Uterine Cervical Cancer with the Risk Factor of HPV: A Focus on Squamous Cell Carcinoma (SCC).

Q3 Medicine

Iranian Journal of Pathology Pub Date : 2025-01-01 Epub Date: 2025-01-10 DOI:10.30699/ijp.2024.2019194.3236

Maryam Fekri Soofi Abadi, Fatemeh Hashemi, Saeedeh Shojaeepour, Sahar Amirpour-Rostami, Mohammad Reza Zangouey, Sara Pourshaikhali, Fatemeh Pouya, Shahriar Dabiri

{"title":"The Association Between RACK1 Gene Polymorphisms rs1279736 and rs3756585 in Uterine Cervical Cancer with the Risk Factor of HPV: A Focus on Squamous Cell Carcinoma (SCC).","authors":"Maryam Fekri Soofi Abadi, Fatemeh Hashemi, Saeedeh Shojaeepour, Sahar Amirpour-Rostami, Mohammad Reza Zangouey, Sara Pourshaikhali, Fatemeh Pouya, Shahriar Dabiri","doi":"10.30699/ijp.2024.2019194.3236","DOIUrl":null,"url":null,"abstract":"Background & objective: Cervical cancer is the second most common malignancy among women worldwide. The Receptor for Activated C Kinase 1 (RACK1) has a key role in regulating the pathways of cell signaling, angiogenesis, cell migration, invasion, and metastasis. This study investigated the association of polymorphisms rs1279736 and rs3756585 with cervical cancer.Methods: In this study, 100 LB pap-smear and paraffin block samples of cervical squamous carcinoma associated with Human papillomavirus (HPV) were selected along with 100 LB smear cytology samples from healthy women with normal pap smears, serving as the control group. Genomic DNA was extracted from the samples, and the polymorphisms rs1279736 and rs3756585 in the RACK1 genes were examined using the RFLP-PCR and ARMS-PCR methods, respectively.Results: The rs1279736 polymorphism shows that the chance of cervical cancer in individuals with CA and AA genotypes is 3.772 and 1.852 times that of CC genotypes, respectively. This suggests that the A allele is involved in developing cervical cancer (P=0.005; OR=2.113). The rs3756585 polymorphism indicates that the chance of cervical cancer in TG and GG genotypes is 0.373 and 4.235 times that of TT genotypes, respectively. This also points towards the involvement of the G allele in the development of cervical cancer (P=0.032; OR=1.800). Also, there was no significant relationship between the genotype of polymorphisms, age, and subtype of papillomavirus 16 and 18 in patients.Conclusion: The present study was conducted for the first time. Our results showed that two polymorphisms are significantly associated with cervical cancer.","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"20 1","pages":"24-32"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11887637/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Pathology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.30699/ijp.2024.2019194.3236","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/10 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Background & objective: Cervical cancer is the second most common malignancy among women worldwide. The Receptor for Activated C Kinase 1 (RACK1) has a key role in regulating the pathways of cell signaling, angiogenesis, cell migration, invasion, and metastasis. This study investigated the association of polymorphisms rs1279736 and rs3756585 with cervical cancer.

Methods: In this study, 100 LB pap-smear and paraffin block samples of cervical squamous carcinoma associated with Human papillomavirus (HPV) were selected along with 100 LB smear cytology samples from healthy women with normal pap smears, serving as the control group. Genomic DNA was extracted from the samples, and the polymorphisms rs1279736 and rs3756585 in the RACK1 genes were examined using the RFLP-PCR and ARMS-PCR methods, respectively.

Results: The rs1279736 polymorphism shows that the chance of cervical cancer in individuals with CA and AA genotypes is 3.772 and 1.852 times that of CC genotypes, respectively. This suggests that the A allele is involved in developing cervical cancer (P=0.005; OR=2.113). The rs3756585 polymorphism indicates that the chance of cervical cancer in TG and GG genotypes is 0.373 and 4.235 times that of TT genotypes, respectively. This also points towards the involvement of the G allele in the development of cervical cancer (P=0.032; OR=1.800). Also, there was no significant relationship between the genotype of polymorphisms, age, and subtype of papillomavirus 16 and 18 in patients.

Conclusion: The present study was conducted for the first time. Our results showed that two polymorphisms are significantly associated with cervical cancer.

查看原文本刊更多论文

子宫颈癌RACK1基因多态性rs1279736和rs3756585与HPV危险因素的关系：以鳞状细胞癌（SCC）为例

背景与目的：宫颈癌是世界范围内女性中第二常见的恶性肿瘤。活化C激酶1受体（RACK1）在调节细胞信号传导、血管生成、细胞迁移、侵袭和转移等途径中起关键作用。本研究探讨了rs1279736和rs3756585多态性与宫颈癌的关系。方法：本研究选取宫颈鳞癌伴人乳头瘤病毒（HPV）的LB涂片和石蜡块标本100例，同时选取宫颈涂片正常的健康女性的LB涂片细胞学标本100例作为对照组。提取样本基因组DNA，分别采用RFLP-PCR和ARMS-PCR方法检测RACK1基因rs1279736和rs3756585的多态性。结果：rs1279736多态性显示CA和AA基因型个体宫颈癌的发生几率分别是CC基因型个体的3.772倍和1.852倍。这表明A等位基因与宫颈癌的发生有关(P=0.005；或= 2.113)。rs3756585多态性提示TG和GG基因型的宫颈癌发生几率分别是TT基因型的0.373和4.235倍。这也表明G等位基因参与宫颈癌的发展(P=0.032；或= 1.800)。此外，在患者中，多态性基因型、年龄和乳头瘤病毒16和18亚型之间没有显著关系。结论：本研究为首次开展。我们的研究结果表明，两个多态性与宫颈癌显著相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊