Iranian Journal of Pathology最新文献_第10页

Cerebrospinal Fluid Analysis in Patients with Post-neurosurgical Procedures: Meningitis vs. Non-meningitis. 神经外科手术后患者脑脊液分析：脑膜炎与非脑膜炎

Iranian Journal of Pathology Pub Date : 2024-01-01 Epub Date: 2024-07-24 DOI: 10.30699/IJP.2023.2019741.3240

Mehdi Zeinalizadeh, Maryam Shadkam, Pegah Afarinesh Khaki, Alireza Abdollahi, Masoumeh Douraghi, Mohammadreza Salehi

{"title":"Cerebrospinal Fluid Analysis in Patients with Post-neurosurgical Procedures: Meningitis vs. Non-meningitis.","authors":"Mehdi Zeinalizadeh, Maryam Shadkam, Pegah Afarinesh Khaki, Alireza Abdollahi, Masoumeh Douraghi, Mohammadreza Salehi","doi":"10.30699/IJP.2023.2019741.3240","DOIUrl":"10.30699/IJP.2023.2019741.3240","url":null,"abstract":"Background & objective: Cerebrospinal fluid (CSF) analysis is helpful in the diagnosis of infections of the central nervous system (CNS), especially after neurosurgical procedures. This study aimed to evaluate the diagnostic value of CSF markers for diagnosis of post-neurosurgical meningitis (PNM).Methods: Patients with neurosurgical procedures whose CSF was obtained for any reason (meningitis and non-meningitis) during 2020 and 2022, at Imam Khomeini Hospital Complex, Tehran, Iran, were included. Serum and CSF lactate dehydrogenase (LDH), glucose, protein, white blood cells (WBC), red blood cells (RBC), and CSF/serum glucose and LDH ratio were compared between the patients who were diagnosed with PNM and those without meningitis.Results: A total of 115 patients were included, of whom 23 patients were diagnosed with PNM and 92 with non-meningitis. No significant differences were observed in patients' age, gender, and underlying diseases between the two groups. Findings showed a significantly (P=0.029) lower level of the mean CSF glucose (59.5 mg/dL ±33.9) in patients with meningitis than in patients without meningitis (76.8 mg/dL ± 37.5). The mean CSF/serum glucose ratio was 43.7% in the meningitis group and 56.3% in the non-meningitis group (P=0.008). The mean WBC count and neutrophil dominance were significantly higher in the meningitis group. No significant differences were observed in CSF LDH, Protein, and RBC between the two groups.Conclusion: A CSF glucose level of less than 60 mg/dL, a CSF/serum glucose ratio of less than 0.44, and a higher CSF WBC and neutrophil count can help diagnose PNM.","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"19 3","pages":"342-347"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11646203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinicopathological Spectrum of Diabetic and Non-diabetic Renal Lesions in Patients with Diabetes Mellitus: An Experience from A Tertiary Care Center. 糖尿病患者的糖尿病和非糖尿病肾病的临床病理谱：来自三级保健中心的经验。

Iranian Journal of Pathology Pub Date : 2024-01-01 Epub Date: 2024-07-24 DOI: 10.30699/IJP.2024.2024462.3270

Netra Prakash Kori, Manjunath Revanasiddappa, Nagraj D Naik, Atul Desai, Ranjana Shashidhar Ranade

{"title":"Clinicopathological Spectrum of Diabetic and Non-diabetic Renal Lesions in Patients with Diabetes Mellitus: An Experience from A Tertiary Care Center.","authors":"Netra Prakash Kori, Manjunath Revanasiddappa, Nagraj D Naik, Atul Desai, Ranjana Shashidhar Ranade","doi":"10.30699/IJP.2024.2024462.3270","DOIUrl":"10.30699/IJP.2024.2024462.3270","url":null,"abstract":"Background & objective: Diabetic patients often develop lesions called non-diabetic renal diseases (NDRD), whose prognostic and therapeutic implications vary from diabetic nephropathy (DN). Since early identification of NDRD is associated with a better prognosis, we aimed to understand its spectrum.Methods: One hundred and thirty-four patients were included in a cross-sectional study. Their clinical, and laboratory data and indications for biopsy were recorded. Two cores of renal tissue were studied under light microscopy and immunofluorescence; patients were classified into NDRD, NDRD+DN, and DN groups.Results: Of all the patients studied, five were diagnosed with type 1 diabetes mellitus (DM1), and the rest were type 2 diabetes mellitus (DM2). Overall, the male-to-female ratio was 3:1. The Mean age of NDRD patients was the greatest, and males were predominant in all three groups. CKD was the most common presentation. Except for significantly greater proteinuria and hematuria in the DN and NDRD groups respectively, others were not different. Infection-related glomerulonephritis was the most common lesion among the NDRD+DN group, whereas IgA nephropathy and acute tubulointerstitial nephritis were frequent in the NDRD group.Conclusion: Based on our findings, renal biopsy should be considered in all those diabetic patients with lesser onset duration presenting with hematuria, no/minimal retinopathy, and minimal proteinuria. A precise diagnosis helps in providing timely therapy for NDRD and prolonging renal and patient survival.","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"19 3","pages":"311-317"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11646201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macrolide-Lincosamide Resistance and Virulence Genes in Staphylococcus aureus Isolated from Clinical Specimens in Ardabil, Iran 伊朗阿达比尔地区金黄色葡萄球菌大环内酯-利可沙胺耐药及毒力基因分析

Iranian Journal of Pathology Pub Date : 2023-10-01 DOI: 10.30699/ijp.2023.1987077.3049

Meysam Manouchehrifar, Farzad Khademi, Hadi Peeri Doghaheh, shahram Habibzadeh, Mohsen Arzanlou

{"title":"Macrolide-Lincosamide Resistance and Virulence Genes in Staphylococcus aureus Isolated from Clinical Specimens in Ardabil, Iran","authors":"Meysam Manouchehrifar, Farzad Khademi, Hadi Peeri Doghaheh, shahram Habibzadeh, Mohsen Arzanlou","doi":"10.30699/ijp.2023.1987077.3049","DOIUrl":"https://doi.org/10.30699/ijp.2023.1987077.3049","url":null,"abstract":"Background & Objective: Staphylococcus aureus causes various hospital- and community-acquired infections. This study aimed to investigate the phenotypic and genotypic characteristics of erythromycin and inducible clindamycin resistance, virulence gene profiles, and spa types of S. aureus isolates collected from patients in Ardabil Province, Iran.Methods: A total of 118 clinical S. aureus isolates, including 50 (42.4%) methicillin-resistant S. aureus (MRSA) and 68 (57.6%) methicillin-susceptible S. aureus (MSSA) strains, were investigated. Resistance patterns were determined by the disk diffusion method and minimum inhibitory concentration (MIC) test. Inducible macrolide-lincosamide-streptogramin B (iMLSB) resistance was detected using D-test method. The polymerase chain reaction (PCR) was used to identify the virulence and resistance-encoding genes. Additionally, the spa types of the isolates were determined using the PCR, followed by sequencing.Results: In total, 49.1% (58/118) and 44% (52/118) of the isolates were resistant to erythromycin and clindamycin, respectively. Overall, 13.5% (16/118) of the isolates showed the iMLSB resistance phenotype. The ermC gene (72.4% [42]) was the most frequent erythromycin resistance-encoding gene, followed by ermA (60.3% [35]), ermB (60.3% [35]), ermTR (51.7% [30]), and msrA (15.5% [9]) genes among erythromycin-resistant isolates. The virulence genes hla, hld, sea, LukS PV, tst, seb, sed, eta, sec, and etb were detected in 93.2%, 74.5%, 70.3%, 32.2%, 29.6%, 17%, 8.5%, 8.5%, 5.9%, and 4.2% of the isolates, respectively. Ten different spa types were identified for 58 erythromycin-resistant S. aureus strains, of which t030 and t078 types were the most common types.Conclusion: A high frequency of macrolide- and lincosamide-resistant S. aureus isolates with different genetic backgrounds of resistance and virulence may be found in patients in Ardabil Province, Iran.","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"220 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136168217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic Resistance Pattern in Intensive Care Units in a Large Referral Hospital in Iran 伊朗一家大型转诊医院重症监护病房的抗生素耐药性模式

Iranian Journal of Pathology Pub Date : 2023-10-01 DOI: 10.30699/ijp.2023.1990807.3073

Samaneh Salarvand, Alireza Abdollahi, Pegah Afarinesh Khaki, Mahsa Norouzi Shadehi, Mohammad Taghi Beigh Mohammadi, Seyed Amir Miratashi Yazdi, Elham Nazar

{"title":"Antibiotic Resistance Pattern in Intensive Care Units in a Large Referral Hospital in Iran","authors":"Samaneh Salarvand, Alireza Abdollahi, Pegah Afarinesh Khaki, Mahsa Norouzi Shadehi, Mohammad Taghi Beigh Mohammadi, Seyed Amir Miratashi Yazdi, Elham Nazar","doi":"10.30699/ijp.2023.1990807.3073","DOIUrl":"https://doi.org/10.30699/ijp.2023.1990807.3073","url":null,"abstract":"Background & Objective: Antibiotic resistance, especially in the form of multidrug-resistant (MDR), is a big problem, especially in intensive care units (ICUs). This study aimed to evaluate antibiotic resistance and MDR patterns among patients hospitalized in the ICUs in one of the large referral centers in Iran.Methods: The present study was conducted at Imam Khomeini Hospital in Tehran (a great referral hospital), which admits critically ill patients requiring ICU services. To determine the rate of positive cultures for resistant strains, the patient’s blood specimens were sent to the laboratory of the hospital for inoculation on proper culture media within 2 hours of extraction. Antimicrobial susceptibility tests were done using the Bauer–Kirby disk diffusion method.Results: A total of 1,755 samples were collected from the patients to assess microbial strains and antibiotic resistance. The most common microbial strains detected in the cultures extracted from peripheral blood samples were Klebsiella pneumonia (22.1%), Staphylococcus epidermidis (7.9%) and another coagulase-negative Staphylococcus (15.0%). The antibiogram test showed antibiotic resistance in 1,509 cases, leading to a resistance prevalence rate of 85.9%. The most common antimicrobial resistance observed was against cotrimoxazole (61.7%), ciprofloxacin (51.3%), imipenem (50.0%), and ampicillin (49.6%). The rate of MDR was found to be 96.3%.Conclusion: In Iran’s ICUs, a significantly high level of antibiotic resistance may be seen especially the MDR pattern, which indicates the need to change the pattern of prescribing and managing these drugs in ICU centers.","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"135 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136168219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different Isoforms of PML-RARA Chimeric Protein in Patients with Acute Promyelocytic Leukemia: Survival Analysis per Demographic Characteristics, Clinicohematological Parameters, and Cytogenetic Findings 急性早幼粒细胞白血病患者PML-RARA嵌合蛋白的不同亚型:人口统计学特征、临床血液学参数和细胞遗传学结果的生存分析

Iranian Journal of Pathology Pub Date : 2023-10-01 DOI: 10.30699/ijp.2023.2007229.3145

Sarah Siahbani, Akbar Safaie, Masoumeh Faghih, Marzieh Hosseini, Afsaneh Fendereski, Behnaz Valibeigi, Ahmad Monabati

{"title":"Different Isoforms of PML-RARA Chimeric Protein in Patients with Acute Promyelocytic Leukemia: Survival Analysis per Demographic Characteristics, Clinicohematological Parameters, and Cytogenetic Findings","authors":"Sarah Siahbani, Akbar Safaie, Masoumeh Faghih, Marzieh Hosseini, Afsaneh Fendereski, Behnaz Valibeigi, Ahmad Monabati","doi":"10.30699/ijp.2023.2007229.3145","DOIUrl":"https://doi.org/10.30699/ijp.2023.2007229.3145","url":null,"abstract":"Background & Objective: Acute Promyelocytic Leukemia (APL) is a medical emergency with potentially fatal complications. APL primarily results from a chromosomal translocation (t(15;17)(q22;q21)), leading to the formation of the PML-RARA fusion gene with three possible isoforms. This study aims to investigate the characteristics of Iranian APL patients, the distribution of PML-RARA isoforms, and survival analysis.Methods: We included 145 consecutive eligible patients in this study. Data were collected through archived documents and phone inquiries, following consent. Subsequently, we analyzed the data using SPSS software version 26.0.Results: We examined 75 men and 70 women, with a mean age of 34 years (range: 2-78 years). Besides t(15;17) (q22;q21), 45.6% had other chromosomal abnormalities. The prevalence of bcr1 and bcr3 isoforms was 73% and 27%, respectively. bcr3 correlated with higher white blood cell (WBC) counts, additional chromosomal abnormalities, and faster Complete Hematologic Response (CHR). Early death occurred in approximately 36% of all patients. The mean overall survival time was 73.5 months, with 120-month survival rates of 53.8% for all patients and 83.9% for those who achieved CHR. Univariate analysis identified old age, relapse, lower platelet (PLT) counts, higher WBC counts, and leukocytosis as survival risk factors. However, in multivariate analysis, only old age and higher WBC counts were identified as adverse prognostic factors.Conclusion: In Iranian APL patients, bcr1 predominates, while bcr3 correlates with higher WBC counts, high-risk categorization, additional chromosomal abnormalities, and faster CHR. Survival is negatively impacted by old age, relapse, lower PLT counts, higher WBC counts, and leukocytosis.","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136168223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Etiology, Clinical Manifestations, Diagnosis, Follow-up, Histopathology and Prognosis Factors in Papillary Thyroid Microcarcinoma: A Systematic Review and Meta-analysis 甲状腺乳头状微癌的病因、临床表现、诊断、随访、组织病理学和预后因素的评价:一项系统综述和荟萃分析

Iranian Journal of Pathology Pub Date : 2023-10-01 DOI: 10.30699/ijp.2023.2005196.3134

Shiva Didehban, Alireza Abdollahi, Alipasha Meysamie

{"title":"Evaluation of Etiology, Clinical Manifestations, Diagnosis, Follow-up, Histopathology and Prognosis Factors in Papillary Thyroid Microcarcinoma: A Systematic Review and Meta-analysis","authors":"Shiva Didehban, Alireza Abdollahi, Alipasha Meysamie","doi":"10.30699/ijp.2023.2005196.3134","DOIUrl":"https://doi.org/10.30699/ijp.2023.2005196.3134","url":null,"abstract":"Background & Objective: The most frequent type of cancer found in the endocrine system is thyroid carcinoma. Among well-differentiated thyroid malignancies, the most commonly occurring type is identified as papillary thyroid carcinoma (PTC), which makes up 70-90% of the cases. A subtype of PTC is papillary thyroid microcarcinoma (PTMC), which includes tumors smaller than 10 mm in diameter. Due to the advancements in diagnostic techniques, the incidence of this type of cancer is on the rise. In this study, we aimed to analyze the factors worsening the PTMC prognosis.Methods: In the first step, we searched various databases for the factors affecting this tumor. The relevant articles were collected and different outcomes of this tumor and its associated factors which were studied in more than one article, were classified. Finally, we conducted a meta-analysis of these outcomes and their related factors.Results: In the meta-analysis, a significantly association was found between the following factors: recurrence with gender (P<0.001) lymph node metastasis (LNM) (P= 0.003), and extrathyroidal invasion (P<0.001); lymph node metastasis with extrathyroidal invasion (P<0.001), and multifocality (P<0.001); central lymph node metastasis (CLNM) with gender (P=0.001), tumor size (P<0.001), extracapsular invasion (P<0.001), lateral cervical lymph node metastasis (P<0.001), and extrathyroidal invasion (P<0.001); lymph node metastasis resulted in poor outcomes (P<0.001); and finally tumor size with BRAFV600E mutation (P<0.001).Conclusion: In conclusion, it is essential to note that greater awareness and understanding of this tumor characteristics and special and separate attention to PTMC can significantly improve the society overall health.","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136168614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of the Milan System for Reporting Salivary Gland Cytology: A Prospective Study 应用米兰系统报告唾液腺细胞学:一项前瞻性研究

Iranian Journal of Pathology Pub Date : 2023-10-01 DOI: 10.30699/ijp.2023.1999632.3098

Reema Bhushan, Jyoti Shrivastava, Varsha Verma

{"title":"Application of the Milan System for Reporting Salivary Gland Cytology: A Prospective Study","authors":"Reema Bhushan, Jyoti Shrivastava, Varsha Verma","doi":"10.30699/ijp.2023.1999632.3098","DOIUrl":"https://doi.org/10.30699/ijp.2023.1999632.3098","url":null,"abstract":"Background & Objective: The Milan system of classification of the salivary gland lesions came up with an aim to establish a universal reporting protocol. The aim of this study was to classify the fine-needle aspiration cytology (FNAC) cases of salivary gland according to the Milan system.Methods: All the cases presenting with salivary gland lesion for FNAC were considered. The clinical data was recorded. Cytology findings were analyzed according to the Milan System. Histopathological correlation was made wherever available.Results: A total of 100 cases of salivary gland lesions were collected and categorized according to the Milan system. They were correlated with histopathology in 45 cases. The patients’ age varied from 2-85 years. Parotid gland was the most commonly affected. Category 1 (non-diagnostic) comprised of three cases. Category 2 (non-neoplastic) had 40 cases. In category 4a (benign) there were 43 cases, and the most common lesion was pleomorphic adenoma. Category 5 (suspicious of malignancy) comprised of 3 cases. Category 6 (malignant) comprised of 11 cases and the most common lesion was mucoepidermoid carcinoma. In category 2, the cytological findings of 5 cases were concordant with histopathology while, 2 were discordant. In category 4a (benign), 20 cases were concordant, and 3 cases were discordant (2 cases were mucoepidermoid carcinoma, 1 was adenoid cystic carcinoma on histology). The risks of malignancy in NN, AUS, benign, SOM, and malignant were 33.3, 2.5, 0, 7, 66.6, and 100%, respectively.Conclusion: Milan system of reporting salivary gland cytopathology may have great potential of escalating clinical communication and may guide appropriate treatment.","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136168214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transitional Metaplasia of Renal Collecting Ducts in Vesicoureteral Reflux and Chronic Pyelonephritis: An Immunohistochemical Study of a Case and Review of the Literature 膀胱输尿管反流合并慢性肾盂肾炎的肾集管移行性化生:1例免疫组织化学研究及文献复习

Iranian Journal of Pathology Pub Date : 2023-10-01 DOI: 10.30699/ijp.2023.1998769.3087

Naser Tayyebi Meibodi, Salman Soltani, Farnaz Torabian

{"title":"Transitional Metaplasia of Renal Collecting Ducts in Vesicoureteral Reflux and Chronic Pyelonephritis: An Immunohistochemical Study of a Case and Review of the Literature","authors":"Naser Tayyebi Meibodi, Salman Soltani, Farnaz Torabian","doi":"10.30699/ijp.2023.1998769.3087","DOIUrl":"https://doi.org/10.30699/ijp.2023.1998769.3087","url":null,"abstract":"The capability of the urinary tract to undergo metaplastic changes such as squamous, intestinal, glandular, mucinous, or ciliated epithelium in renal pelvis has been previously reported, which hypothetically is due to the mechanical irritation of the transitional epithelium. However, transitional metaplasia is a rare presentation in the collecting ducts. The aim of this paper was to report this type of extremely rare metaplasia and to inform pathologists that they may encounter this kind of metaplasia. A 25-year-old man, a known case of vesicoureteral reflux (VUR), referred to the Imam Reza Hospital; affiliated to the Mashhad University of Medical Sciences, for bilateral nephrectomy. Gross evaluation of bilateral nephrectomy specimens showed atrophic kidneys and dilated pelvicalyceal systems. The light microscopic evaluation showed transitional metaplasia in the background of chronic pyelonephritis, confirmed by GATA3 nuclear immunohistochemical stain. In this study, we presented a rare case of a renal collecting duct with transitional epithelial lining replacing the normal epithelium as a metaplastic change, with the hypothesis that previous medical history including VUR, or hemodialysis could be the trigger for the metaplastic change, which should be confirmed by further studies.","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"2012 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136168617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of HER2 Positivity Based on Clinicopathological Findings in HER2 Borderline Tumors in Iranian Patients with Breast Cancer 基于伊朗乳腺癌患者HER2交界性肿瘤临床病理结果评价HER2阳性

Iranian Journal of Pathology Pub Date : 2023-10-01 DOI: 10.30699/ijp.2023.561915.2970

Ramesh Omranipour, Newsha Nazarian, Sadaf Alipour, Alireza Abdollahi, Bita Eslami

{"title":"Evaluation of HER2 Positivity Based on Clinicopathological Findings in HER2 Borderline Tumors in Iranian Patients with Breast Cancer","authors":"Ramesh Omranipour, Newsha Nazarian, Sadaf Alipour, Alireza Abdollahi, Bita Eslami","doi":"10.30699/ijp.2023.561915.2970","DOIUrl":"https://doi.org/10.30699/ijp.2023.561915.2970","url":null,"abstract":"Background & Objective: Human epidermal growth receptor-2 (HER2) gene amplification is an important predictive and prognostic factor in breast cancer treatment. However, the expression of HER2 determined by immunohistochemistry (IHC) is considered as borderline in some cases, and confirmation of the HER2 status by either fluorescent in situ hybridization (FISH) or chromogenic in situ hybridization (CISH) is necessary for correct treatment decision-making. Considering the high cost of FISH and CISH, we aimed to investigate whether clinicopathological findings of the tumor could predict the HER2 status. Methods: A retrospective study was performed using the data from 584 patients with breast cancer with HER2-borderline disease, confirmed by IHC. Final HER2 status, pathologic tumor size and type, nodal involvement, Ki67 index, presence of estrogen and progesterone receptors (ER, PR), lymphovascular invasion (LVI), and stage were retrieved from the clinical records.Results: One hundred twenty-one (20.7%) patients were HER2-positive according to the FISH or CISH results. Logistic regression analysis showed that the pathologic size was positively associated with HER2 positivity with an odds ratio (OR) of 1.02 (95% CI: 1.01-1.04). In addition, the adjusted OR illustrated a statistically significant association between HER2 positivity and PR negativity (OR= 2.22, 95% CI: 1.29-3.83).Conclusion: In HER2 borderline breast cancer, HER2 positivity significantly increases with tumor size and PR negativity. Further studies are recommended that may find an applicable model to predict the actual status of HER2 in borderline cases.","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136167888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing a Multiplex PCR for Rapid and Accurate Detection of Metallobetalactamases Resistant Genes from Acinetobacter baumaniiIsolates in Tehran City, Iran 设计一种快速准确检测伊朗德黑兰鲍曼不动杆菌金属β -内酰胺酶耐药基因的多重PCR方法

Iranian Journal of Pathology Pub Date : 2023-10-01 DOI: 10.30699/ijp.2023.2007142.3144

zahra Mottaghiyan, Davoud Esmaeili, Mohammad Ahmadi, Mohammad Niakan

{"title":"Designing a Multiplex PCR for Rapid and Accurate Detection of Metallobetalactamases Resistant Genes from Acinetobacter baumaniiIsolates in Tehran City, Iran","authors":"zahra Mottaghiyan, Davoud Esmaeili, Mohammad Ahmadi, Mohammad Niakan","doi":"10.30699/ijp.2023.2007142.3144","DOIUrl":"https://doi.org/10.30699/ijp.2023.2007142.3144","url":null,"abstract":"Background & Objective: Acinetobacter baumannii strains harboring Meallobetalactamases (MBL) pose a significant threat in the context of nosocomial infections. The present investigation was undertaken with the objective of devising a Multiplex PCR methodology for the concurrent detection of MBL genes within A. baumannii strains prevalent in Tehran City, Iran.Methods: Between October 2020 and February 2021, 100 strains of A. baumannii were procured from burn specimens of hospitalized patients at Motahhari Hospital in Tehran. The identification of A. baumannii strains involved conventional biochemical techniques, coupled with confirmation of the presence of the bla OXA-51 gene. Antibiotic susceptibility was assessed using the Kirby–Bauer disc diffusion test. MBL-producing strains were characterized through a phenotypic approach employing the combined disk test, alongside Multiplex PCR for the simultaneous identification of bla VIM, bla IMP, bla GIM, and bla NDM genes. Statistical analyses were conducted using the chi-square test, with SPSS version 20.0 employed for data processing.Results: Among 100 strains examined, 96.1% exhibited positivity for MBL, as determined by the combined disk test. The study revealed a predominance of extensively drug-resistant (XDR) strains, with colistin demonstrating the highest level of sensitivity. The genotypic assay unveiled that Multiplex PCR identified bla VIM, bla NDM, and bla IMP in 20 strains, bla VIM and bla NDM in 30 strains, and exclusively the bla NDM gene in 45 strains. Notably, the Multiplex PCR technique exhibited the capacity to concurrently detect MBL genes (bla VIM, bla IMP, bla GIM, bla NDM) in 2 strains.Conclusion: The current investigation underscores prevalence of the bla NDM gene within clinical strains of A. baumannii. Furthermore, Multiplex PCR emerges as a robust and highly sensitive technique for rapid discernment of the MBL genes within in A. baumannii strains.","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136168216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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