Iranian Journal of Pathology最新文献

{"title":"α-Smooth Muscle Actin as Predictors of Early Recurrence in Early-Stage Ductal Type Breast Cancer After Mastectomy and Chemotherapy.","authors":"Andreas Andrianto, I Ketut Sudiana, Desak G A Suprabawati","doi":"10.30699/IJP.2023.2004468.3126","DOIUrl":"10.30699/IJP.2023.2004468.3126","url":null,"abstract":"<p><strong>Background & objective: </strong>Breast cancer recurrence after surgery was a sign that the progress of the disease was continuing. Early detection of breast cancer patients who are at risk requires development of a marker. Alfa smooth muscle actin (α-SMA) plays a role in the local recurrence process of invasive ductal carcinoma (IDC). Currently, existing tumor markers are used to predict the prognosis of breast cancer in general, not the early stages. Therefore, it was thought that finding α-SMA expression might predict early recurrence in early-stage IDC more accurately than others. This study investigated the potential role of α-SMA expression as a predictor of early recurrence in early-stage IDC and its relationship to clinicopathological factors.</p><p><strong>Methods: </strong>The study design was cross-sectional, with data obtained from the medical records of Dr. Koesnadi, General Hospital, Bondowoso, Indonesia. Bivariate and multivariate analysis was performed to analyze data.</p><p><strong>Results: </strong>We included 50 subjects divided into the local recurrence group (n=25) and the non-local recurrence group (n=25). We found a statistically significant correlation between the incidence of local recurrence in early-stage IDC and the high expression of α-SMA (odd ratio [OR]=23.22, 95% confidence interval [CI]=5.101-105.7, <i>P</i>=0.001). Clinicopathological variables and α-SMA expression did not have a significant correlation.</p><p><strong>Conclusion: </strong>In early-stage IDC, α-SMA expression had the potential to predict and could be an independent prognostic factor for early recurrence.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"19 1","pages":"67-74"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11164303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141307065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytoplasmic Expression of p16 Is Associated with Carcinoma Breast: It Is Not an Artifact.","authors":"Sudipta Naskar, Nadeem Tanveer, Sonal Sharma, Navneet Kaur","doi":"10.30699/IJP.2024.2006691.3186","DOIUrl":"10.30699/IJP.2024.2006691.3186","url":null,"abstract":"<p><strong>Background & objective: </strong>p16 has different roles in the nuclear and cytoplasmic locations. The nuclear localization of the p16 protein explains its role in cell cycle regulation. Cytoplasmic expression was considered an artifact in the initial years, but there is evidence to prove that cytoplasmic localization is real and that p16 has different roles in the nuclear and cytoplasmic locations. We aimed to study the immunoexpression of p16 protein in the nuclear and cytoplasmic locations of the epithelial and stromal compartments of fibroadenoma, invasive breast carcinoma, and a select number of phyllodes tumors.</p><p><strong>Methods: </strong>The study included a total of 107 patients, comprising 51 cases of invasive breast carcinoma, 51 cases of fibroadenoma, 4 cases of benign phyllodes tumors, and 1 case of lobular carcinoma in situ (LCIS). The p16 immunohistochemistry was evaluated for nuclear and cytoplasmic localization in the epithelial and stromal compartments of the tumors.</p><p><strong>Results: </strong>Of the 51 fibroadenoma cases, 23 showed strong nuclear p16 epithelial expression, but no case showed cytoplasmic expression. In 19/51 cases, stromal cells also showed strong p16 nuclear expression. Moderate stromal p16 expression was observed in 3 out of 4 cases of benign phyllodes. Out of the 51 cases of invasive carcinoma, 31 showed moderate to strong nuclear p16 immunopositivity, while 27 cases exhibited cytoplasmic p16 expression. We found a statistically significant correlation between moderate to strong nuclear p16 immunoexpression and the molecular subtype of breast carcinoma.</p><p><strong>Conclusion: </strong>The cytoplasmic localization of p16 immunohistochemistry is not seen in epithelial components of fibroadenoma, while it is seen frequently in breast carcinoma. Nuclear p16 expression has a statistically significant correlation with molecular subtypes of breast carcinoma.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"19 2","pages":"160-169"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11304457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological Evaluation of PYGO2 Expression in Esophageal Squamous Cell Carcinoma.","authors":"Sima Ardalan Khales, Habibeh Rahmani Kalat, Sedigheh Soleymani, Amir Hossein Jafarian, Mohammad Mahdi Forghanifard","doi":"10.30699/ijp.2024.2024609.3269","DOIUrl":"10.30699/ijp.2024.2024609.3269","url":null,"abstract":"<p><strong>Background & objective: </strong>Esophageal squamous cell carcinoma (ESCC) is one of the world's deadliest cancer diseases. Deregulation of developmental signaling pathways such as Wnt/β-catenin is frequently implicated in a wide range of human cancers. The present study was designed to analyze the expression of the Pygopus2 (PYGO2) protein, the main co-activator of the Wnt/β-catenin signaling pathway, in ESCC tissues and evaluate its probable correlation with clinicopathological features of patients.</p><p><strong>Methods: </strong>In this study, PYGO2 protein expression was assessed in tumors and margin normal tissues from 50 ESCC patients using immunohistochemical analysis, and its clinicopathological relevance in the patients was evaluated.</p><p><strong>Results: </strong>A significant PYGO2 overexpression was observed in %32 of the tumor cells. Interestingly, PYGO2 expression was significantly correlated with the depth of tumor invasion (<i>P</i>= 0.021).</p><p><strong>Conclusion: </strong>PYGO2 protein may be highly expressed in ESCC in correlation with the invasiveness of the disease. Therefore, it may be used as a biomarker for diagnosis of invasive ESCC and a putative therapeutic target to inhibit ESCC invasiveness.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"19 4","pages":"415-521"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical Expression of Programmed Death Ligand- 1 (PD-L1) in Colorectal Carcinoma; A Cross-sectional Study.","authors":"Shruti Tadachina, Sheela Devi Shivalingaiah, Mahesh Shetty","doi":"10.30699/IJP.2023.1988660.3054","DOIUrl":"10.30699/IJP.2023.1988660.3054","url":null,"abstract":"<p><strong>Background & objective: </strong>Colorectal carcinoma (CRC) is one of the most common cancers worldwide. The interaction of programmed cell death receptor 1 (PD-1) and programmed death ligand 1 (PD-L1) plays an important role by inhibiting the immune mechanism by which cancer cells escape antitumor immunity. Immunotherapy using checkpoint inhibitors is a growing treatment modality in many cancers; one such is anti-PD1/PD-L1. The present study aimed to study the immunohistochemical (IHC) expression of PD-L1 in CRC and its association with various known clinicopathological parameters.</p><p><strong>Methods: </strong>This study was a 2-year prospective study and included 34 colectomy specimens diagnosed as colorectal adenocarcinoma. The expression of PD-L1 was evaluated on tumoral cells and tumor-infiltrating immune cells (TIICs) and was correlated with various clinicopathological parameters.</p><p><strong>Results: </strong>Immunohistochemical expression of PD-L1 on tumoral cells and tumor microenvironment in CRC revealed positivity in 17.65% of cases each. The PD-L1 expression on tumoral cells was associated with lymphovascular invasion (LVI) and perineural invasion (PNI) with P- values of 0.012 and 0.005, respectively, while PD-L1 expression on TIICs was associated with tumor budding with a P-value of 0.022.</p><p><strong>Conclusion: </strong>IHC expression of PD-L1 on tumoral cells and immune cells may be associated with some known poor prognostic factors. Since anti-PD1/PD-L1 is used for targeted therapy, it may be beneficial and economically feasible to evaluate PD-L1 in CRC and establish its role as a prognostic factor.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"19 1","pages":"22-30"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11164304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141307059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of CK7 Immunoreactivity in Predicting Progression of the Cervical Intraepithelial Neoplasia: A Retrospective Cross-sectional Study.","authors":"Soheila Sarmadi, Fatemeh Nili, Kiana Anousha","doi":"10.30699/IJP.2024.2019662.3239","DOIUrl":"10.30699/IJP.2024.2019662.3239","url":null,"abstract":"<p><strong>Background & objective: </strong>Cervical intraepithelial squamous lesions (SIL) are the first step toward developing most cervical cancers. Moreover, most of the previous studies focusing on a reliable predictive factor of cervical cancer progression have been reported as inconclusive. Recently, immunophenotyping of squamocolumnar junction cells with CK7 has been introduced as a novel SIL detection and risk evaluation method. Therefore, we aimed to investigate the value of CK7 positivity and its pattern in predicting the progression of LSIL to high-grade counterparts.</p><p><strong>Methods: </strong>This retrospective study was conducted in Yas Complex Hospital from 2016 to 2018 among patients of the clinic of colonoscopy. Patients with low-grade lesions were included in one group, and patients with high-grade lesions were considered as a control group. The immunoreactivity of CK7 by immunohistochemical staining pattern was interpreted in both groups.</p><p><strong>Results: </strong>CK7 immunoreactivity was negative in 29.5% of the precancerous lesions and positive in 70.5%. The relationship between grade and CK7 staining was significant (<i>P</i> = 0.040). A significant relationship between the progression status of the disease and CK7 staining was obtained (<i>P</i>=0.001). CK7 staining showed a sensitivity of 82.35%, specificity of 81.82%, positive predictive value of 87.5%, negative predictive value of 75%, and diagnostic accuracy of 82.14% in predicting the progression of the SILs.</p><p><strong>Conclusion: </strong>Our data and previous studies support the correlation of CK7 expression with a higher chance of progression of low-grade intraepithelial lesions.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"19 4","pages":"384-391"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of Tumor Vascular Microenvironment by Nestin and Fms-related Tyrosine Kinase 1 (FLT1) and Their Prognostic Significance in Renal Cell Carcinoma.","authors":"Noha Elkady, Reham Ahmed Abdelaziz, Rasha Adel Abdelmoneum, Ahmed S Ghonaimy, Dina Mohamed Allam","doi":"10.30699/IJP.2024.2024190.3266","DOIUrl":"10.30699/IJP.2024.2024190.3266","url":null,"abstract":"<p><strong>Background & objective: </strong>Hypervascularity is a characteristic feature of renal cell carcinoma (RCC) and microvessel density (MVD) predicts tumor metastasis. Nestin is a stem cell marker that is expressed in proliferating endothelial cells and newly formed vessels and Fms-related tyrosine kinase 1 (FLT1) is a proangiogenic factor. This study aimed to evaluate the expression of Nestin and FLT1 in RCC and their prognostic impact.</p><p><strong>Methods: </strong>This retrospective study included sixty cases of RCC after obtaining ethical approval. Sections were immunohistochemically stained by Nestin and FLT1 then their expressions were compared to different clinicopathological parameters. MVD was evaluated using Nestin and CD34 and compared to the different parameters.</p><p><strong>Results: </strong>Nestin was expressed mainly in endothelial cells of small vessels in 65% of cases while FLT1 was expressed in tumor and endothelial cells in 73.3% of cases. Their expressions were significantly associated with aggressive tumor parameters including larger tumors, high-grade tumors, wider tumor extension, and advanced stage. Moreover, Nestin expression was significantly associated with metastasis. MVD evaluated by Nestin showed more associations with larger tumors, high-grade tumors, wider tumor extension, advanced stage, and metastasis than MVD measured by CD34. Nestin and FLT1 positivity and high MVD measured by Nestin were significantly associated with short overall survival.</p><p><strong>Conclusion: </strong>Nestin and FLT1 expressions in RCC may be associated with aggressive tumor features and short patients' overall survival. MVD evaluated by Nestin may be correlated with tumor progression and metastasis. Nestin and FLT1 may be used as prognostic biomarkers in RCC.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"19 3","pages":"332-341"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11646198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FABP4, GINS2 and CBX7 Expression in Cancer Cervix Tissues: Clinical, Pathological and Prognostic Implications.","authors":"Mariem A Elfeky, Rema H Faraj Saad, Mohamed Ali Alabiad, Mohammed Alorini, Rehab Hemeda, Ramadan M Ali, Loay M Gertallah, Mohamed Negm, Ahmed Mahmoud Abdou, Ahmed Baker A Alshaikh, Ahmed Elmaasrawy","doi":"10.30699/IJP.2023.1971325.2944","DOIUrl":"10.30699/IJP.2023.1971325.2944","url":null,"abstract":"<p><strong>Background & objective: </strong>Cervical cancer spreads to the pelvic lymph nodes, leading to a high incidence of cancer recurrence and unfavorable survival rates. Therefore, there is an urgent need to detect new predictive biomarkers for the early assessment of pelvic lymph node status in patients with cervical cancer. The current study aimed to assess the expression of FABP4, GINS2, and CBX7 in cervical cancer tissue to detect their prognostic and predictive roles in developing lymph node metastases in patients with that cancer type.</p><p><strong>Methods: </strong>We collected the tissues from patients with cervical cancer and evaluated the expression of FABP4, GINS2, and CBX7 using immunohistochemistry. We evaluated the association between their expression and clinicopathological and prognostic parameters.</p><p><strong>Results: </strong>A high expression of FABP4 and GINS2 and a low expression of CBX7 were found to be positively associated with the old age group, large tumor size, high grade and lymphovascular involvement, para-uterine organ infiltration, advanced FIGO stage, chemotherapeutic resistance, and tumor recurrence.</p><p><strong>Conclusion: </strong>We demonstrated the oncogenic roles of FABP4 and GISN2 in addition to the on-co-suppressive roles of CBX7 in cervical cancer and their association with poor clinicopathological criteria and poor survival. Our results may indicate that FABP4, GISN2, and CBX7 could be considered predictive biomarkers of the occurrence of lymph node metastases in the cancer of the cervix preoperatively, which could be beneficial in the accurate preoperative design therapy.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"19 1","pages":"10-21"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11164314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary Undifferentiated Pleomorphic Sarcoma of the Breast: A Case Report with Literature Review of Similar Cases.","authors":"Amirhossein Jafarian, Mohaddeseh Shahraki, Samaneh Sajjadi, Behrooz Daneshmand","doi":"10.30699/IJP.2023.2006411.3139","DOIUrl":"10.30699/IJP.2023.2006411.3139","url":null,"abstract":"<p><p>Breast sarcoma is a rare but aggressive tumor. There are few case reports in the literature and several aspects of this disease are still not completely comprehended. Therefore, reporting new cases can help to enrich the literature. We report a patient with breast mass and pus secretion from her right breast, misdiagnosed as an abscess and mistreated by antibiotics. The patient was referred for an ultrasound examination and mammography, and a needle biopsy was performed that suggested an aggressive tumor. By the pathologist's suggestion, a total mastectomy of the right breast was performed with the excision of sentinel nodes. A pathological examination revealed a high-grade undifferentiated pleomorphic sarcoma (UPS) without vascular or lymph node invasion as the final diagnosis. The patient underwent postoperative chemotherapy and is currently in good condition. This case emphasizes considering this rare tumor when approaching a breast mass. Performing surgery with adequate resection margin can improve the patient's prognosis. Some suggested breast UPS cases with lung and brain metastasis would be more aggressive tumors than other breast sarcomas. Total mastectomy with negative margins and free-of-tumor lymph nodes may be the key to improve prognosis in such patients.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"19 1","pages":"118-125"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11164308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141307061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategic Framework for Strengthening Health Laboratory Services in the COVID-19 Pandemic in Iran.","authors":"Nooshafarin Safadel, Parisa Dahim, Siamak Mirab Samiee, Katayoon Khodaverdian, Maryam Mir Mohammad Ali Roodaki, Soghra Anjarani, Shahla Farsi, Mahdi Rafiee, Marjan R Farzami","doi":"10.30699/IJP.2024.2013702.3192","DOIUrl":"10.30699/IJP.2024.2013702.3192","url":null,"abstract":"<p><strong>Background & objective: </strong>Providing equitable access to good quality, timely, and affordable laboratory testing has always been a top priority for the Ministry of Health and Medical Education (MoH-ME) and the Reference Health Laboratory (RHL). Considering the significant role of medical laboratories in disease surveillance, RHL developed a strategic plan to manage laboratory services during the COVID-19 pandemic based on the \"Strategic Framework for strengthening health laboratory services, 2016-2020\" proposed by the World Health Organization (WHO). This article describes the steps taken to establish the strategic framework in Iran.</p><p><strong>Methods: </strong>Firstly, a National Laboratory Committee was formed in MoH-ME and a situation analysis was conducted to explore the strengths, weaknesses, opportunities, and threats in different components of our laboratory system. Gaps and resources needed to address those gaps were determined; then, RHL outlined operational processes and mechanisms for monitoring the activities.</p><p><strong>Results: </strong>The WHO strategic roadmap and its six strategic goals concerning leadership, quality, human resources, safety and security, laboratory networking, and rational use of laboratory testing, helped us to promote national laboratory services in accordance with health system requirements in the COVID-19 pandemic.</p><p><strong>Conclusion: </strong>The establishment of a national molecular laboratory network with more than 500 laboratories from different sectors may result in timely access to countrywide laboratory services and would be beneficial for future COVID-19 and/or other viral outbreaks. Continual evaluation of the COVID-19 laboratories' performance, production of PCR test kits by the local manufacturers, and development of a platform for virtual training would be other accomplishments that Iran achieved in coping with the recent pandemic.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"19 2","pages":"170-176"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11304460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Mir-1290 as a Possible Diagnostic Factor in the Serum of Oral Squamous Cell Carcinoma Patients with Qualitative Real-Time Polymerase Chain Reaction.","authors":"Siavash Bagheri Shirvan, Mehdi Shahabinejad, Farnaz Mohajertehran, Nazanin Nazari","doi":"10.30699/IJP.2024.2010130.3161","DOIUrl":"10.30699/IJP.2024.2010130.3161","url":null,"abstract":"<p><strong>Background & objective: </strong>This study aimed to determine the incidence of microRNA (miRNA; miR-1290) in the serum of oral squamous cell carcinoma (OSCC) patients compared to a control group using the qualitative real-time polymerase chain reaction (PCR) method.</p><p><strong>Methods: </strong>Blood serum samples were obtained from patients diagnosed with OSCC and confirmed through biopsy. The samples were collected from patients referred to the Mashhad Dental Faculty and Ghaem Hospital. The OSCC group consisted of 17 patients, while the healthy group included 15 individuals. RNA was extracted from the patient samples, and samples with an A260/280 ratio between 1.8 and 2.0 (indicating acceptable RNA quality) were immediately converted into complementary DNA (cDNA) using albumin and cDNA reference genes. The SYBR green real-time reverse transcriptase PCR method was used to measure the presence of miR-1290 in the blood samples.</p><p><strong>Results: </strong>A total of 32 patients were examined in this study, including 17 women (53.1%) and 15 men (46.9%). The mean age was 46.7 years in the healthy group and 54.6 years in the SCC group, indicating a significant difference (<i>P</i><0.05). The expression level of the miR-1290 gene was higher in patients with SCC compared to the healthy group (<i>P</i>=0.000). While the expression level of miR-1290 was higher in grade 3 and advanced stage than in grades 2 and 1 and early stage, the differences were not statistically significant (<i>P</i>=0.173 and <i>P</i>=0.564 for grade and stage, respectively).</p><p><strong>Conclusion: </strong>The expression level of miR-1290 may increase in SCC patients compared to healthy individuals, making it a potential circulating biomarker. Further investigations for diagnostic utility would be warranted.</p>","PeriodicalId":38900,"journal":{"name":"Iranian Journal of Pathology","volume":"19 2","pages":"244-249"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11304459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}