Journal of Oral and Maxillofacial Pathology最新文献

{"title":"Papillon-Lefevre syndrome: Case series.","authors":"Vasumati I Patel, Karan R Thakkar, Shalini S Gupta, Meghna J Pujara","doi":"10.4103/jomfp.jomfp_25_24","DOIUrl":"10.4103/jomfp.jomfp_25_24","url":null,"abstract":"<p><p>Papillon-Lefevre syndrome (PLS) is an exceptional autosomal recessive disorder characterized by palmoplantar keratinization and premature loss of deciduous and permanent teeth. The palmoplantar keratoderma commonly has its onset between the ages of 1 and 4 years with severe periodontitis initiating at 3 or 4 years old. Parental consanguinity has been demonstrated in 20% to 40% of the cases. Management and preservation of teeth in such patients is a challenge to periodontists. Genetic studies have shown that mutation in the major gene locus of chromosome 11q14.1-q14.3 with the loss of function of the Cathepsin-C (CTSC) gene is responsible for PLS. An early diagnosis of the syndrome can help preserve the teeth through early institution of treatment, using a multidisciplinary approach. We hereby present four cases having all the characteristic features of the syndrome.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"28 4","pages":"694-700"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11819644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of Fascin-1 with clinicopathological parameters and recurrence in oral squamous cell carcinoma.","authors":"Fatema Saify, Minal Chaudhary, Nidhi Tiwari, Shilpa Jain, Sarbani Deb Sikhdar, Meenakshi Sood","doi":"10.4103/jomfp.jomfp_146_24","DOIUrl":"10.4103/jomfp.jomfp_146_24","url":null,"abstract":"<p><strong>Background: </strong>Squamous cell carcinoma is the most common epithelial malignancy of the oral cavity. The 5-year survival rate is approximately 50% and is even lower, that is, 30% in the patients with recurrence of this disease. Because recurrence has a major influence on 5-year survival, it becomes imperative to identify the molecular elements responsible for the recurrence of oral squamous cell carcinoma.</p><p><strong>Aim: </strong>To evaluate the expression of fascin-1 and its correlation with clinicopathological parameters and recurrence in the patients of oral squamous cell carcinoma.</p><p><strong>Materials and methods: </strong>A cohort study was conducted in the Department of Oral Pathology, SPDC, Wardha. A 4 μm thick section from paraffin-embedded blocks of formalin-fixed biopsy tissues of 60 cases having primary oral squamous cell carcinoma was processed and stained with mouse monoclonal fascin-1 (Clone: SPM133) antibodies.</p><p><strong>Results: </strong>In the present study, all cases which had a final score above 2, denotes a strong positive immunostaining of fascin in OSCC. Tumour size was significantly correlated with fascin expression, whereas no significant association was found for other parameters, such as age, gender, node involvement, and grade of the tumour and recurrence.</p><p><strong>Conclusion: </strong>Fascin was found to be significantly correlated with tumour size. Fascin expression was found to be related with recurrence but no significant association exists. As recurrence is directly related to the poor prognosis and survival rate; so, it is essential to identify molecular markers indicative of recurrence. Fascin can be used to identify a subset of oral squamous cell carcinoma patients that are prone to recurrence.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"28 4","pages":"555-559"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11819638/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-biofilm effect of clove oil against <i>Candida albicans</i>: A systematic review.","authors":"J Palaskar Sangeeta, O Balsaraf Aishwarya, D Balsaraf Omkar, N Bhave Madhura","doi":"10.4103/jomfp.jomfp_225_24","DOIUrl":"10.4103/jomfp.jomfp_225_24","url":null,"abstract":"<p><p>In recent years, <i>Candida albicans</i> has emerged as a significant opportunistic pathogen, causing various infections, particularly in immunocompromised geriatric individuals. One of the major hindrances in managing <i>Candida</i> infections lies in its potential to develop and survive in biofilms, which demonstrate resistance to conventional antifungal therapies, thereby complicating treatment strategies. While clove oil has shown promising results as a potential antifungal agent, its specific anti-biofilm effects against <i>Candida albicans</i> biofilms remain relatively underexplored. Therefore, there is a need for a comprehensive review to evaluate the existing literature on the anti-biofilm properties of clove oil and its mechanisms of action against <i>Candida albicans</i> biofilms. Such a review will provide valuable insights into the potential therapeutic applications of clove oil in combating biofilm-associated <i>Candida</i> infections. This review will enable us to infer and understand the potential effects of clove oil against <i>Candida albicans</i>. This systematic review aims to determine the anti-biofilm efficacy of clove oil against <i>Candida albicans</i>. This systematic review was registered in 'PROSPERO' (CRD42024524134) and followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The literature searches used Google Scholar, PubMed, and Science Direct databases. All original studies published in English from 2004 to 2023 were included. From 37 studies found online, only four were reviewed. Results showed that clove oil had significant anti-biofilm activity when assessed against <i>Candida albicans</i>. Our systematic review provides insights into clove oil's potential as a therapeutic agent against <i>Candida albicans</i> biofilms. Clove oil also represents a promising avenue for future research and development in managing <i>Candida biofilms</i>.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"28 4","pages":"665-671"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11819647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histomorphometric analysis of lichen planus and lichenoid lesions - Retrospective study.","authors":"Manti Vijayalakshmi, Bandela Rajasekhar, Vishwaprakash Shetty, Animelli Jacob Prakash, Thokala Madhusudhan Rao, H Aparna Latha","doi":"10.4103/jomfp.jomfp_29_24","DOIUrl":"10.4103/jomfp.jomfp_29_24","url":null,"abstract":"<p><strong>Introduction: </strong>Though it has a different aetiology, lichenoid lesions and lichen planus are both chronic inflammatory mucocutaneous conditions with similar clinical and histological characteristics. Uncertainty persists despite the development of recommendations for distinguishing oral lichen planus from lichenoid diseases.</p><p><strong>Materials and methodology: </strong>A retrospective analysis was conducted on 25 cases of oral lichen planus (OLP), oral lichenoid lesions (OLL), and normal oral mucosa (NOM) that had been histologically diagnosed. Using imagej analysis software, morphometric assessments of the cellular area, nuclear area, nucleus cytoplasmic ratio, nuclear diameter, and cellular diameter of suprabasal cells were performed on sections stained with haematoxylin and eosin. The data were then analysed using ANOVA.</p><p><strong>Results: </strong>Our study's findings demonstrated that, in comparison to the normal oral mucosa, the mean values of the nuclear area, cellular area, nuclear diameter, and cellular diameter in oral lichen planus and oral lichenoid lesions were higher.</p><p><strong>Conclusion: </strong>In the current investigation, we also found that, in comparison to normal mucosa, the nuclear-cytoplasmic ratio was lower in oral lichen planus and oral lichenoid lesions.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"28 4","pages":"570-575"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11819632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of DNA, in dried blood, saliva tinged with blood in comparision with fresh blood for forensic identification- A pilot study.","authors":"Kangan Manjunathan, Radhika Manoj Bavle, Mrinalini Menon, Reshma Venugopal, Soumya Makarla, Sankha Nair","doi":"10.4103/jomfp.jomfp_137_24","DOIUrl":"10.4103/jomfp.jomfp_137_24","url":null,"abstract":"<p><p>The important tenet of forensic science is personal identification based on DNA studies. The use of DNA has changed the specificity and sensitivity of victim identity tests associated with criminology, trauma, and natural disasters in Forensic Science. Blood is a proven source of DNA quantification for these analyses. The use of saliva, which can be acquired more easily in a simple and atraumatic method is gaining preference; as some of its advantages include - collection of larger sample numbers, easy replication of tests in duplicates and triplicates, and repetitions if advocated, can easily be performed. Though it yields a comparatively slightly lesser quantity of DNA, some of the samples - like dried blood and blood-tinged saliva, have the potential to be used in forensic sciences and provide a better result than simple saliva. Such samples are valuable sources of genetic material that can be utilized in crime and forensic investigations. The present study is one of few studies to be explored in this sphere as very few studies using blood-tinged saliva and dried blood have been conducted so far.</p><p><strong>Aims and objectives: </strong>To determine if blood-tinged saliva or dried blood yields a good amount of DNA. To compare it with fresh blood samples for authentication.</p><p><strong>Materials and methodology: </strong>The study groups: Group A - <i>n</i> = 20 normal blood samples, Group B - <i>n</i> = 20 of saliva-tinged blood samples and Group C - Dried Blood samples from the same subjects. DNA was isolated from these 60 samples using Xplorogen DNA isolation and extraction kit. The quality and quantity of DNA isolation were determined using a spectrophotometric technique using a Biospectrometer.</p><p><strong>Results: </strong>The extracted DNA was quantified and the yield was 83.3 ± 9.4, 55.1 ± 7.4, and 73.4 ± 8.7 μg/ml in fresh blood, dried blood, and blood-tinged saliva, respectively. Out of the 60 samples assessed, all 100% samples yielded a sufficiently good DNA content. Extraction from Blood tinged with saliva showed promising results when compared to dried blood and the levels obtained were closely comparable to whole blood (fresh).</p><p><strong>Conclusion: </strong>Blood is a standard sample for ample DNA yields in forensic studies. We conclude in this study, that blood-tinged saliva gives an equally good sample for utilization in DNA detection in those situations where such samples are traced (even at a later date) at the scene of crime, or mass disasters for forensic analysis.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"28 4","pages":"725-730"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11819636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The oral-lung microbiome dysbiosis: Unravelling its role in implications for chronic obstructive pulmonary disease (COPD) pathogenesis.","authors":"Nimisha Mithradas, Uma Sudhakar, K Shanmugapriya, Nadeem Jeddy, Shankar Ram","doi":"10.4103/jomfp.jomfp_277_24","DOIUrl":"10.4103/jomfp.jomfp_277_24","url":null,"abstract":"<p><strong>Background: </strong>The impact of the oral flora on the composition of the microbiome in the lungs is substantial in both healthy and diseased conditions, contributing significantly to its intricacy. There is mounting evidence from microbiological research that suggests a major ecological relationship between periodontitis, Chronic Obstructive Pulmonary Disease (COPD), and oral microecosystems. An association has been established between respiratory diseases and disruptions in the symbiotic equilibrium of the oral microbiome. This study aims to explore the intricate connections between oral health and lung microflora, particularly about the pathogenesis of COPD, and to highlight the implications for future research and clinical practice.</p><p><strong>Materials and methods: </strong>Subgingival Plaque samples were collected from a total of 120 participants with 30 healthy Control (H group),30 Periodontitis with no COPD (P group), 30 COPD with periodontally healthy (COPD) and 30 individuals with COPD and Periodontitis (COPD+ P). All participants underwent evaluation of periodontal measurements like Pocket Depth (PD), Clinical loss of Attachment (CAL), Gingival Index (GI), and Plaque Index (PI) Bacterial DNA was extracted and quantified using Real-time polymerase chain reaction. Using the One-dimensional Analysis of Variance (ANOVA) and post-analysis test for multiple comparisons, the mean values of all the clinical parameters were analyzed among the four participant groups. Using the Pearson Correlation coefficient, the parameters were correlated.</p><p><strong>Results: </strong>Statistical relevant relation was shown among Probing Depth (PD), Clinical Loss of Attachment (CAL), Plaque Index (PI) and Gingival Index (GI) in the COPD+P group. Increased prevalence of Pa (<i>Pseudomonas aeroginosa</i>) seen among <i>P</i> group and COPD+P. A substantial inverse relationship was seen between the absolute levels of Pa, CAL, PI, and lung function measures (Fev1, Fev1/FVC).</p><p><strong>Conclusion: </strong>The importance of maintaining dental health in the prevention and treatment of respiratory disorders is highlighted by the relationships that exist between the oral microecosystem, oral hygiene, and respiratory pathologies. There is substantial potential to decrease the occurrence of respiratory illnesses by practicing good oral care and strategically managing the balance of the oral microbial flora. Therefore, future research efforts should prioritize the characterization of the precise impact of the oral microbiota on pulmonary health and use this knowledge towards developing innovative preventive and treatment measures targeted at combating respiratory infections and related diseases.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"28 4","pages":"619-625"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11819631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive analysis of odontogenic tumors according to recent WHO (2022) classification: An institution-based retrospective study.","authors":"Piyush Asnani, Shireen Ali, Sima Odedra, Jayasankar Pillai, Namrata Jayasheel, Ranjit Jadeja","doi":"10.4103/jomfp.jomfp_56_24","DOIUrl":"10.4103/jomfp.jomfp_56_24","url":null,"abstract":"<p><strong>Background: </strong>Odontogenic tumors constitute a rare group of lesions with diverse characteristics originating from tooth-forming tissues, primarily within the maxillofacial region. Variations in incidence across populations in India and with changes incorporated in successive World Health Organization (WHO) classifications, necessitate continued research to understand their epidemiology.</p><p><strong>Objective: </strong>This retrospective study aims to provide an extensive analysis of Odontogenic tumors reported in a dental institute in Gujarat, India, using the 5^th edition of the WHO classification. The study also seeks to compare the findings with data from other regions.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on archived histopathological records from the Oral Pathology department of our institute, spanning from 1966 to 2023. This analysis focused on demographic details, tumor types, and their distribution. The data were reclassified according to the WHO 5^th edition and subjected to descriptive statistical analysis.</p><p><strong>Results: </strong>Among 10,907 biopsies, 501 cases (4.6%) were diagnosed as Odontogenic tumors, with 97.6% being benign. Benign epithelial tumors, predominantly Ameloblastoma (68.9%) was most common. Supporting their rare occurrence malignant cases included Ameloblastic carcinoma (2.2%) and Clear Cell Odontogenic Carcinoma (0.2%). An overall male predominance (51.7%) was noted, with female dominance in Adenomatoid Odontogenic tumor (60%), Cemento-Ossifying Fibroma (62.5%), Odontogenic fibroma (91%), and Odontogenic Myxoma (66.7%). Most cases occurred in the third decade (27.1%), with a notable mandibular predominance (78%).</p><p><strong>Conclusion: </strong>The findings contribute to the understanding of Odontogenic tumors epidemiology, emphasizing the importance of further regional studies and adapting to evolving classifications for accurate assessments and cross-population comparisons.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"28 4","pages":"576-582"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11819634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The chronicles of green complex bacteria.","authors":"Moitri Ojha, Amrita, Disha Gupta, Vartika Verma","doi":"10.4103/jomfp.jomfp_121_24","DOIUrl":"10.4103/jomfp.jomfp_121_24","url":null,"abstract":"<p><p>Periodontal pathogens have always captivated the attention of periodontists and microbiologists as it account for causing periodontal disease in 90% of the population globally. Clinical and experimental studies have confirmed that destructive activity on the periodontium is due to certain strains of bacteria that occupy a relatively small portion of dental biofilm. Among them, the green and the red complex bacteria enjoy the popularity of being the most notorious strain in disease initiation and progression. The genera of green complex bacteria comprise three pathogens- <i>Aggregatibacter actinomyecetecomitans</i>, <i>Capnocytophaga</i>, and <i>Eikenella corrodens</i>. The group possesses several stratagems and key elements that aid them in escaping the immune surveillance and creating a harsh environment for the periodontium. The review focuses on defining the green complex bacteria and their role in periodontitis.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"28 4","pages":"633-640"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11819641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of clinical and histopathological changes in leukoplakia using curcumin lozenges based on revised WHO criteria- A pilot study.","authors":"Parul Khare, Anshu Gupta Devra, Madhvika Patidar, Rohit Punga, Nirupama Gupta, Soo-Yeon Sohn","doi":"10.4103/jomfp.jomfp_227_24","DOIUrl":"10.4103/jomfp.jomfp_227_24","url":null,"abstract":"<p><strong>Introduction: </strong>Oral epithelial dysplasia, such as leukoplakia has a risk of malignant transformation. Curcumin, a natural polyphenol with anticancer properties, has been explored for many years as an alternative treatment for leukoplakia.</p><p><strong>Aim: </strong>To evaluate clinical and histopathological changes in oral leukoplakia from curcumin lozenges, this study compares pre- and post-medication effects. It is the first to involve general and oral pathologists from India and the USA, who independently assessed changes using WHO 2022 criteria.</p><p><strong>Material and method: </strong>The study involved 10 patients and was conducted in three phases. Initial biopsy, and administration of curcumin lozenges (2 tablets thrice daily for 3 months) with clinical evaluation every month followed by a second biopsy.</p><p><strong>Results: </strong>Post-medication, clinically in a few cases reduction of lesion size was observed as a change from white to gray patches. Histopathological analysis was subjective and the three experts reported an overall improvement in architectural as well as cytological features suggesting the possible use of curcumin in treating cases of mild dysplasia.</p><p><strong>Conclusion: </strong>The pilot study suggests curcumin lozenges may benefit leukoplakia patients, but the small sample size limits definitive conclusions. Larger studies are needed to confirm these findings and assess long-term efficacy. WHO 2022 criteria showed subjectivity and variability. Despite this, they were useful for identifying specific changes. To the best of our knowledge to date, no other study has validated these WHO 2022 features in repeated biopsies.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"28 4","pages":"626-632"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11819616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of <i>porphyromonas gingivalis</i> in oral potentially malignant disorders and oral squamous cell carcinoma using qRT-PCR: A comparative study.","authors":"G Suganya, N S Sahana, P Akalya, Satish T Yadav, T Suresh, J Chandrakala","doi":"10.4103/jomfp.jomfp_119_24","DOIUrl":"10.4103/jomfp.jomfp_119_24","url":null,"abstract":"<p><strong>Background: </strong>Recent researches has shown a significant association between microorganisms and oral squamous cell carcinoma (OSCC). <i>Porphyromonas gingivalis</i>, the keystone pathogen in chronic periodontitis, is considered as an important potential etiologic agent of OSCC, but the underlying mechanisms by which <i>P. gingivalis</i> mediates OSCC progression remain poorly understood.</p><p><strong>Aim: </strong>The aim of this study was to compare the levels of <i>P. gingivalis</i> in oral potentially malignant disorders, oral squamous cell carcinoma and normal oral mucosa using qRT-PCR.</p><p><strong>Method and material: </strong>Genomic DNA was extracted and quantified, and the expression of the <i>P. gingivalis</i> levels was done in 16 cases of oral potentially malignant disorders, 16 cases of oral squamous cell carcinoma and 16 cases of normal oral mucosa by quantitative real-time polymerase chain reaction (RT-qPCR).</p><p><strong>Results: </strong>It was observed that there was an over expression of <i>P. gingivalis</i> in both oral potentially malignant disorders and oral squamous cell carcinoma with good mean cycle threshold (CT) value of 27.00 and 27.55, respectively. When comparing the levels of <i>P. gingivalis</i> in three groups, oral potentially malignant disorders (OPMD) and oral squamous cell carcinoma (OSCC) showed higher expression than normal mucosa and in between two groups OSCC showed higher expression than OPMD and the difference is statistically significant with <i>P</i> value less than 0.001.</p><p><strong>Conclusion: </strong>Our findings suggest that there is an over expression of <i>P. gingivalis</i> in oral potentially malignant disorders and oral squamous cell carcinoma, compared to normal mucosa and highly expressed in OSCCs compared to OPMD. Increased levels of <i>P. gingivalis</i> in OPMDs and OSCCs may suggest the early event of tumorigenesis. Hence, it can be used as a valuable marker for early diagnosis, prognosis marker and in the identification of therapeutic targets.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"28 4","pages":"583-588"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11819619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}