Journal of Oral and Maxillofacial Pathology最新文献

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Forensic DNA analysis of heat-exposed unrestored teeth: Temperature and duration effects. 热暴露未修复牙齿的法医DNA分析:温度和持续时间的影响。
Journal of Oral and Maxillofacial Pathology Pub Date : 2025-07-01 Epub Date: 2025-09-26 DOI: 10.4103/jomfp.jomfp_159_25
C N Roshin, Sudeendra Prabhu, T S Bastian, M Selvamani, H S Suhana, K Ankitha Thejus
{"title":"Forensic DNA analysis of heat-exposed unrestored teeth: Temperature and duration effects.","authors":"C N Roshin, Sudeendra Prabhu, T S Bastian, M Selvamani, H S Suhana, K Ankitha Thejus","doi":"10.4103/jomfp.jomfp_159_25","DOIUrl":"10.4103/jomfp.jomfp_159_25","url":null,"abstract":"<p><strong>Context: </strong>Forensic identification often relies on DNA recovery from human remains, with teeth being a preferred source due to their resistance to extreme conditions.</p><p><strong>Aim: </strong>To evaluate the DNA yield from unrestored teeth exposed to various temperatures and durations and assess its utility in forensic identification.</p><p><strong>Settings and design: </strong>An experimental study assessing DNA recovery from teeth subjected to controlled heat exposure.</p><p><strong>Materials and methods: </strong>Unrestored teeth were exposed to temperatures of 200°C, 450°C, 700°C, 950°C, and 1200°C for 30 and 60 min. DNA was then extracted and quantified.</p><p><strong>Statistical analysis used: </strong>The study analysed DNA yield from unrestored teeth exposed to varying temperatures and durations. Posterior teeth showed higher yields, with DNA decreasing as heat and time increased. Independent <i>t</i>-tests, ANOVA, and Tukey honestly significant difference confirmed statistically significant differences.</p><p><strong>Results: </strong>DNA was effectively recovered at lower temperatures and shorter durations. Higher temperatures and longer exposures reduced DNA yield, highlighting the relevance of thermal conditions in forensic applications.</p><p><strong>Conclusions: </strong>Forensic dentistry is crucial in identifying remains exposed to fire or decomposition. Teeth, especially posteriors, often preserve DNA due to their durability and protected position. They offer a reliable, cost-effective source of genetic material, even in harsh conditions. Advancements in extraction methods continue to improve identification in complex forensic cases.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"29 3","pages":"478-485"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145259449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Minimum ramus breadth and coronoid height in gender determination - An orthopantomographic study. 性别测定中的最小分支宽度和冠状高度。一项骨解剖研究。
Journal of Oral and Maxillofacial Pathology Pub Date : 2025-07-01 Epub Date: 2025-09-26 DOI: 10.4103/jomfp.jomfp_297_24
Reshma Poothakulath Krishnan, Deepak Pandiar, A Mabbithasri, Bianca Princeton, Abirami Arthanari
{"title":"Minimum ramus breadth and coronoid height in gender determination - An orthopantomographic study.","authors":"Reshma Poothakulath Krishnan, Deepak Pandiar, A Mabbithasri, Bianca Princeton, Abirami Arthanari","doi":"10.4103/jomfp.jomfp_297_24","DOIUrl":"10.4103/jomfp.jomfp_297_24","url":null,"abstract":"<p><strong>Background: </strong>Gender determination is an important part of forensic dentistry and legal investigations. Mandible is the largest, sexually dimorphic, and strongest bone of the facial skeleton. Morphometric measurements of the mandible by using orthopantomogram are widely used in gender determination.</p><p><strong>Aim: </strong>The aim of this study was to determine the efficacy of minimum ramus breath and coronoid height of the mandible for gender determination using orthopantomogram.</p><p><strong>Materials and methods: </strong>Sixty orthopantomograms of 30 males and 30 females between the age group of 30 to 40 years were collected. The minimum ramus breath and the coronoid height were measured using a computerized software. The data obtained was statistically analysed using IBM SPSS Statistics for Windows, Version 23.0 (IBM Corp., Armonk, New York, United States) software.</p><p><strong>Results: </strong>The mean minimal ramus breath and mean coronoid height were greater in males than in females. The mean minimal ramus breath for males was found to be 37.83 ± 2.24 and for females, it was 33.08 ± 4.87 (<i>P</i> = 0.00). The mean coronoid height of males was 80.67 ± 0.83 and that of females was 73.51 ± 1.26 (<i>P</i> = 0.00).</p><p><strong>Conclusion: </strong>Our results suggest that the mandible is a sexually dimorphic bone, and the minimum ramus breath and coronoid height can be used in gender determination. However, population specific studies with larger sample sizes are recommended.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"29 3","pages":"468-471"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145259435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimizing outcomes: The role of oral metronomic therapy in proliferative verrucous leucoplakia. 优化结果:口服节奏疗法在增殖性疣状白斑中的作用。
Journal of Oral and Maxillofacial Pathology Pub Date : 2025-07-01 Epub Date: 2025-09-26 DOI: 10.4103/jomfp.jomfp_320_24
Supriya Kheur, Karishma Madhusudan Desai, Nikhil Pande, Vinay K Hazarey
{"title":"Optimizing outcomes: The role of oral metronomic therapy in proliferative verrucous leucoplakia.","authors":"Supriya Kheur, Karishma Madhusudan Desai, Nikhil Pande, Vinay K Hazarey","doi":"10.4103/jomfp.jomfp_320_24","DOIUrl":"10.4103/jomfp.jomfp_320_24","url":null,"abstract":"","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"29 3","pages":"465-467"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145259455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Swertia purpurascens Wall: Evaluation of its antifibrotic activity in an in vitro model of oral submucous fibrosis. 紫癜獐牙菜壁:在口腔粘膜下纤维化的体外模型中评价其抗纤维化活性。
Journal of Oral and Maxillofacial Pathology Pub Date : 2025-07-01 Epub Date: 2025-09-26 DOI: 10.4103/jomfp.jomfp_115_25
Snehalata Narvekar, Shridhar D Baliga, Punnya V Angadi
{"title":"Swertia purpurascens Wall: Evaluation of its antifibrotic activity in an <i>in vitro</i> model of oral submucous fibrosis.","authors":"Snehalata Narvekar, Shridhar D Baliga, Punnya V Angadi","doi":"10.4103/jomfp.jomfp_115_25","DOIUrl":"10.4103/jomfp.jomfp_115_25","url":null,"abstract":"<p><strong>Background: </strong>Oral submucous fibrosis (OSF) is a chronic, progressive, and potentially malignant condition prevalent in Southeast Asia, primarily associated with betel nut consumption. The pathogenesis involves overexpression of transforming growth factor beta 1 (TGF-β1), which drives fibroblast activation and excessive extracellular matrix (ECM) deposition. Despite available therapies, effective long-term management remains elusive.</p><p><strong>Objective: </strong>This study investigates the antifibrotic activity of <i>Swertia purpurascens</i> Wall extract, a xanthone-rich medicinal herb, by evaluating its effect on TGF-β1 expression, fibroblast viability, and ECM remodelling in primary OSF-derived human fibroblasts.</p><p><strong>Methods: </strong>Primary fibroblasts were isolated from OSF biopsy tissues, authenticated through STR profiling, and phenotypically confirmed by vimentin expression using flow cytometry. Cells were treated with <i>Swertia purpurascens</i> extract at concentrations ranging from 31.25 to 500 μg/mL. TGF-β1 secretion was quantified using ELISA. Cell viability was measured via MTT assay over a 72-hour period. Collagen gel contraction assays assessed ECM remodelling capacity.</p><p><strong>Results: </strong>A significant, dose-dependent reduction in TGF-β1 secretion was observed, with the highest concentration (500 μg/mL) achieving nearly 50% inhibition (<i>P</i> < 0.05). Fibroblast viability decreased linearly over time (<i>R</i> <sup>2</sup> = 0.998), indicating sustained cytostatic activity. Post-treatment gel contraction also reduced by approximately 50%, supported by linear regression analysis (<i>R</i> <sup>2</sup> = 0.955).</p><p><strong>Conclusion: </strong><i>Swertia purpurascens</i> Wall extract effectively attenuates fibrosis-related cellular processes in OSF-derived fibroblasts. Its ability to suppress TGF-β1 signalling, limit fibroblast proliferation, and inhibit ECM contraction highlights its potential as a plant-based antifibrotic therapy for OSF and related fibrotic disorders.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"29 3","pages":"402-408"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145259563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of knowledge, attitude, and awareness regarding age estimation using forensic odontology among Karnataka Labour Department Officials: A cross-sectional survey. 卡纳塔克邦劳工部官员对使用法医牙科学估计年龄的知识、态度和意识的评估:一项横断面调查。
Journal of Oral and Maxillofacial Pathology Pub Date : 2025-07-01 Epub Date: 2025-09-26 DOI: 10.4103/jomfp.jomfp_29_25
Krishnanand P Satelur, Vidya M Annegowda, Y B Geetha Priya, Bhanu Prakash
{"title":"Assessment of knowledge, attitude, and awareness regarding age estimation using forensic odontology among Karnataka Labour Department Officials: A cross-sectional survey.","authors":"Krishnanand P Satelur, Vidya M Annegowda, Y B Geetha Priya, Bhanu Prakash","doi":"10.4103/jomfp.jomfp_29_25","DOIUrl":"10.4103/jomfp.jomfp_29_25","url":null,"abstract":"<p><strong>Context: </strong>Child labour remains a significant concern in India, with numerous children engaged in hazardous occupations, often lacking valid age documentation. Forensic odontology, a specialised discipline within dentistry, can play a pivotal role in age estimation and individual identification in such cases. However, its integration into child labour enforcement mechanisms is limited.</p><p><strong>Aim: </strong>To assess the knowledge, attitude, and awareness of Karnataka State Labour Department officials regarding the role of forensic odontology in age estimation related to child labour cases.</p><p><strong>Methods: </strong>A cross-sectional survey was conducted among 80 officials from the Karnataka State Labour Department, each with a minimum of 5 years of experience. A structured questionnaire comprising 20 items assessed knowledge, attitude, and awareness regarding forensic odontology and its relevance to child labour cases. Multivariate data analysis was performed using The Unscrambler X software from CAMO Analytics.</p><p><strong>Results: </strong>While a majority of officials had heard of forensic odontology, their primary source of information was media rather than formal training. Most participants acknowledged the significance of forensic odontology in age estimation and expressed interest in undergoing formal training. However, limited access to resources and lack of training were cited as major barriers to its implementation.</p><p><strong>Conclusion: </strong>The study reveals a significant knowledge and training gap among Labour Department officials regarding forensic odontology. Incorporating structured training programs and promoting interdisciplinary collaboration could enhance the effective application of forensic odontology in child labour cases.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"29 3","pages":"472-477"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145259572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Emmprin (CD147) expression in oral submucous fibrosis and in oral squamous cell carcinoma-A potential predictor. Emmprin (CD147)在口腔黏膜下纤维化和口腔鳞状细胞癌中的表达——一种潜在的预测因子。
Journal of Oral and Maxillofacial Pathology Pub Date : 2025-07-01 Epub Date: 2025-09-26 DOI: 10.4103/jomfp.jomfp_115_23
Rajshri Raveendran, Sivakumar Gopalakrishan, Raghini Ramamoorthi, Sivakumar Muniapillai
{"title":"Emmprin (CD147) expression in oral submucous fibrosis and in oral squamous cell carcinoma-A potential predictor.","authors":"Rajshri Raveendran, Sivakumar Gopalakrishan, Raghini Ramamoorthi, Sivakumar Muniapillai","doi":"10.4103/jomfp.jomfp_115_23","DOIUrl":"10.4103/jomfp.jomfp_115_23","url":null,"abstract":"<p><strong>Introduction: </strong>Oral squamous cell carcinoma arising from oral submucous fibrosis (OSMF) is highly invasive with metastasis and high recurrence rate. WHO has categorized oral carcinoma as 6<sup>th</sup> most common cancer in male and 10<sup>th</sup> most common cancer in female. It is estimated that there are 7-13% chances of malignant transformation of OSMF, with 60% of cases fall under well-differentiated OSCC. CD147 also called as EMMPRIN (Basigin) is a transmembrane glycoprotein and found to be highly expressed by active cells like tumour cells and lymphocytes.</p><p><strong>Aims and objectives: </strong>This study aims at evaluating the expression of EMMPRIN (CD147) in both OSMF and in oral squamous cell carcinoma through immunohistochemical technique, to use EMMPRIN as a marker for disease progression and prognosis.</p><p><strong>Materials and method: </strong>In this study, we have taken 12 samples of OSMF and 12 samples of OSCC along with 5 normal tissue blocks as control. The tissue samples were all retrieved and taken from the Department of Oral Pathology and Microbiology which were reconfirmed by histopathological diagnosis. All the samples were poly-HRP (horse radish peroxidase) and PolyExcel Stunn substrate and chromogen. The staining was scored and analysed statistically.</p><p><strong>Results: </strong>There was a significantly good expression in OSMF with 83.3% of cases, that is, 10 out of 12 show positive staining. Weak positive cases and moderately positive cases were 4 each, and strong positive cases were 2. This indicates that the staining increases as the stage advances. In OSCC, the number of cases showing positive staining was 5, which makes up to 41.7%. As the stage of OSCC increases, the expression of EMMPRIN (CD147) is expected to increase as it is a component important in both angiogenesis and metastasis.</p><p><strong>Conclusion: </strong>The 42% positive CD147 (EMMPRIN) expression in OSCC indicates that it may progress to the next stage of invasion and metastasis, whereas the 83% of positive cases of OSMF indicate that it is likely to transform into OSCC as early as possible. But further study with large sample size and proper distribution of samples in each stage of OSMF and OSCC can help in better analysis of the marker CD147 (EMMPRIN) as a predictor of prognosis.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"29 3","pages":"441-451"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145259345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of pattern of tobacco habit and tobacco associated oral mucosal lesions in Kerala population- An epidemiological study. 喀拉拉邦人群烟草习惯和烟草相关口腔黏膜病变模式的评估-一项流行病学研究。
Journal of Oral and Maxillofacial Pathology Pub Date : 2025-07-01 Epub Date: 2025-09-26 DOI: 10.4103/jomfp.jomfp_25_25
Niveditha Baiju, C P Athira, T S Indu Sundaram, Litu Mary Thampy, Lakshmi Balakrishnan, Aneesa Ameer
{"title":"Assessment of pattern of tobacco habit and tobacco associated oral mucosal lesions in Kerala population- An epidemiological study.","authors":"Niveditha Baiju, C P Athira, T S Indu Sundaram, Litu Mary Thampy, Lakshmi Balakrishnan, Aneesa Ameer","doi":"10.4103/jomfp.jomfp_25_25","DOIUrl":"10.4103/jomfp.jomfp_25_25","url":null,"abstract":"<p><strong>Background: </strong>The use of tobacco significantly affects oral health and causes serious hazards like oral potentially malignant disorders (OPMD) and Oral Cancer. This study analyses the prevalence of various oral mucosal lesions among tobacco users.</p><p><strong>Methods: </strong>A cross-sectional study was conducted using medical records of subjects with various types of tobacco habit reported over a period of five years. The subjects were grouped as smokers, smokeless tobacco users and subjects with mixed habits. A working classification was formulated to include all the tobacco-induced lesions in the study subjects. Three categories of lesions in this classification were Oral Cancer, OPMD and Tobacco-induced oral lesions without malignant potential. The pattern of tobacco use, age and gender distribution, and the correlation between tobacco use in all the three habit patterns with tobacco-induced oral lesions were analyzed statistically and <i>P</i> value was determined using Chi square test.</p><p><strong>Results: </strong>Tobacco smoking was found to be highly prevalent in this study, with all types of tobacco habits being more common among males. Tobacco-induced lesions without malignant potential were more prevalent than oral cancers and OPMD among the group of smokers whereas OPMD were highly prevalent among the smokeless tobacco users and subjects with mixed habit. Statistically significant correlation was found between smokeless tobacco usage and OPMD and other tobacco-induced lesions without malignant potential.</p><p><strong>Conclusion: </strong>The results of this study can be utilised to educate patients with tobacco habits, with the aim of improving oral health and preventing the occurrence of OPMD.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"29 3","pages":"377-381"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145259498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
From the Editor's desk. 从编辑的桌子上。
Journal of Oral and Maxillofacial Pathology Pub Date : 2025-07-01 Epub Date: 2025-09-26 DOI: 10.4103/jomfp.jomfp_274_25
P Jayanthi
{"title":"From the Editor's desk.","authors":"P Jayanthi","doi":"10.4103/jomfp.jomfp_274_25","DOIUrl":"https://doi.org/10.4103/jomfp.jomfp_274_25","url":null,"abstract":"","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"29 3","pages":"343"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145259442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of cytotoxic effects of fruit extracts on oral cancer cell lines: An in vitro experimental study. 水果提取物对口腔癌细胞系细胞毒作用的体外实验研究。
Journal of Oral and Maxillofacial Pathology Pub Date : 2025-07-01 Epub Date: 2025-09-26 DOI: 10.4103/jomfp.jomfp_328_24
Samyukta Srinivasan, R Sathish Muthukumar, N Nachiammai Nachiappan, Sreeja Chellaswamy, V L Premika Sri
{"title":"Evaluation of cytotoxic effects of fruit extracts on oral cancer cell lines: An <i>in vitro</i> experimental study.","authors":"Samyukta Srinivasan, R Sathish Muthukumar, N Nachiammai Nachiappan, Sreeja Chellaswamy, V L Premika Sri","doi":"10.4103/jomfp.jomfp_328_24","DOIUrl":"10.4103/jomfp.jomfp_328_24","url":null,"abstract":"<p><strong>Background: </strong>The Indian subcontinent has the highest incidence and prevalence of Oral Squamous cell carcinoma. Recently, there has been a shift in paradigm favoring natural products to combat cancer, owing to the high costs and immense side effects associated with the conventional treatments such as surgery, radiotherapy, and chemotherapy. Cancer statistics express that consumption of diets rich in fresh fruits and vegetables is inversely associated with cancer incidence. The same has led to the increased number of studies in this area in recent times. The cytotoxic effect of many fruit extracts on various cancers, like cervical cancer, breast cancer, and colon cancer, has been reported.</p><p><strong>Aim: </strong>To evaluate the cytotoxic effect of <i>Vaccinium macrocarpon</i> (cranberry) and <i>Punica granatum</i> (pomegranate) extracts on oral cancer cell lines.</p><p><strong>Materials and methods: </strong>Ethanolic extract of <i>Punica granatum</i> pericarp, fruit, and <i>Vaccinium macrocarpon</i> fruit were prepared. Oral cancer (KB) cell line was procured and cultured. Anti- proliferative assay (MTT assay) was performed with various concentrations of all three fruit extracts on oral cancer. Percentage of cell viability for each concentration was calculated, and the half maximal inhibitory concentration (IC50) was derived for the extracts. The role of apoptosis in the cytotoxicity of these extracts was assessed by analysing DNA fragmentation via gel electrophoresis. The data obtained was analysed using Pearson's correlation.</p><p><strong>Results: </strong>The study revealed a significant decrease in the percentage of viable cells with increasing concentration of the extract. The IC50 value of <i>Punica granatum</i> pericarp, fruit, and <i>Vaccinium macrocarpon</i> extract was 5.625 μg/mL, 15 μg/mL, and 27.5 μg/mL, respectively. On comparison, <i>Punica granatum</i> pericarp extract showed the highest anti-carcinogenic activity. The DNA fragmentation assay showed a DNA laddering pattern, indicative of apoptosis, in the cells treated with <i>Punica granatum</i> pericarp extract.</p><p><strong>Conclusion: </strong>Punic granatum pericarp, fruit, and <i>Vaccinium macrocarpon</i> exhibited sufficient anticancer activity against oral cancer (KB) cells. Apoptosis was shown to play a role in the cytotoxic effect of <i>Punica granatum</i> pericarp extract against oral cancer cells.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"29 3","pages":"395-401"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145259334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the effectiveness of oral Pirfenidone in the treatment of oral submucous fibrosis: A non-randomised pilot clinical trial. 评价口服吡非尼酮治疗口腔粘膜下纤维化的有效性:一项非随机临床试验。
Journal of Oral and Maxillofacial Pathology Pub Date : 2025-07-01 Epub Date: 2025-09-26 DOI: 10.4103/jomfp.jomfp_319_24
Vedashree Mahajan, Bhakti Patil, Deepa Das
{"title":"Evaluation of the effectiveness of oral Pirfenidone in the treatment of oral submucous fibrosis: A non-randomised pilot clinical trial.","authors":"Vedashree Mahajan, Bhakti Patil, Deepa Das","doi":"10.4103/jomfp.jomfp_319_24","DOIUrl":"10.4103/jomfp.jomfp_319_24","url":null,"abstract":"<p><strong>Background: </strong>Oral submucous fibrosis (OSMF) is a potentially malignant disorder with a high rate of malignant transformation. Pirfenidone is an anti-fibrotic drug currently used in the treatment of idiopathic lung fibrosis (ILF). As both ILF and OSMF are mediated through transforming growth factor-beta, Pirfenidone could be beneficial in treating early stages of OSMF.</p><p><strong>Objectives: </strong>To evaluate the effectiveness of oral Pirfenidone in increasing the mouth opening, reducing fibrosis and burning sensation.</p><p><strong>Settings and design: </strong>A single-arm prospective clinical trial conducted in the Department of Oral Medicine and Radiology, at a recognised dental college.</p><p><strong>Materials and methods: </strong>Eleven subjects of either sex, of 21-55 years diagnosed with OSMF Stage 2 according to Chandramani More <i>et al</i>. classification, were recruited for the study and prescribed Pirfenidone 200 mg, 1 tablet TID with meals for 1 week, and later 2 tablets TID with meals for 5 weeks. The patients were monitored for adverse effects, and treatment response was assessed as unit of improvement. Data were analysed using SPSS; parametric tests such as ANOVA followed by <i>post hoc</i> test and non-parametric tests such as Friedman's followed by Wilcoxon signed rank test were applied with significance at <i>P</i> < 0.05.</p><p><strong>Results: </strong>60% of the patients showed +1 unit of improvement with no serious adverse effect.</p><p><strong>Conclusions: </strong>The patient-related clinical outcomes were quite significant showing the effectiveness of Pirfenidone as a promising adjuvant therapy in the management of OSMF.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"29 3","pages":"424-429"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145259380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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