Journal of Oral and Maxillofacial Pathology最新文献

{"title":"Evaluation of salivary vimentin in oral erosive lichen planus, oral leukoplakia, oral squamous cell carcinoma and healthy subjects using ELISA.","authors":"O Arjun, R Rathy, A Neena","doi":"10.4103/jomfp.jomfp_342_25","DOIUrl":"10.4103/jomfp.jomfp_342_25","url":null,"abstract":"<p><strong>Context: </strong>Oral cancer is the sixth most common cancer globally. In India, 80% of oral cancers are preceded by oral potentially malignant disorders (OPMDs). The conditions with mild dysplasia go unrecognised and may eventually transform into severe dysplasia and ultimately to malignant lesions. Hence, the development of biomarkers that will serve as an adjunct to histological diagnosis has become essential.</p><p><strong>Aim: </strong>The present study undertaken to assess the salivary levels of vimentin, being non-invasive as early biomarker for malignant transformation of OPMDs.</p><p><strong>Settings and design: </strong>Cross-sectional study, participants were selected from the Departments of Oral Medicine and Radiology and Oral Pathology and Microbiology of Azeezia College of Dental Sciences and Research, Kollam.</p><p><strong>Methods and materials: </strong>The salivary vimentin expression levels were detected using ELISA and were correlated with oral erosive lichen planus, oral leukoplakia, oral squamous cell carcinoma (OSCC) patients and healthy individuals. The association of salivary vimentin levels with clinical severity of oral erosive lichen planus, clinical types of leukoplakia, and clinical staging of OSCC. and also, the relationship between different grades of epithelial dysplasia in leukoplakia patients and histopathological grades of OSCC was assessed.</p><p><strong>Statistical analysis used: </strong>Data were analysed using the statistical package SPSS 26.0 (SPSS Inc., Chicago, IL, USA). Descriptive statistics were performed to estimate the mean, standard deviation, proportion of the respective groups. Kruskal-Wallis test followed by Tukey's HSD <i>post hoc</i> test was used to compare the differences between the groups. Independent <i>T</i>-test was used for comparison of groups.</p><p><strong>Results: </strong>The results showed that salivary vimentin can be used as a biomarker for predicting malignant transformation of oral leukoplakia and oral erosive lichen planus. The study showed a statistically significant correlation between oral erosive lichen planus, oral leukoplakia and oral cancer and control groups regarding salivary vimentin levels, also there is a statistically significant correlation of salivary vimentin with OSCC histopathological grades and OSCC Clinical Staging.</p><p><strong>Conclusion: </strong>The present study showed that salivary vimentin can be used as a biomarker for predicting malignant transformation of oral leukoplakia and oral erosive lichen planus.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"30 2","pages":"287-294"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148698047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crosstalk between salivary citrullinated proteins and oral health status in patients with rheumatoid arthritis: A cross-sectional study.","authors":"Nandita Kulkarni, Varsha Vimal Kumar, S Chandrashekara, Suraj Sathyendra, Girish Hemadala Channabasavaiah, Mayukh Dey","doi":"10.4103/jomfp.jomfp_341_25","DOIUrl":"10.4103/jomfp.jomfp_341_25","url":null,"abstract":"<p><strong>Context: </strong>Rheumatoid arthritis (RA) is often associated with reduced salivary flow, increasing susceptibility to opportunistic oral infections. Evidence suggests shared pathogenic mechanisms between RA and periodontitis. As a non-invasive medium, saliva offers potential for evaluating associations between anti-citrullinated protein antibody, <i>Porphyromonas gingivalis</i>, and RA.</p><p><strong>Aims: </strong>To measure salivary citrullinated proteins in RA patients, detect <i>P. gingivalis</i> and <i>Candida</i> species, and assess associated oral changes.</p><p><strong>Settings and design: </strong>Cross-sectional study including 21 RA patients and 21 age- and gender-matched healthy controls.</p><p><strong>Materials and methods: </strong>Unstimulated saliva was collected for citrullinated protein estimation using enzyme-linked immunosorbent assay and cultured on Sabouraud dextrose agar for <i>Candida</i> quantification. Subgingival plaque samples were analysed for <i>P. gingivalis</i>. Oral status was assessed using the Community Periodontal Index (CPI) and decayed, missing, and filled teeth (DMFT) index.</p><p><strong>Statistical analysis used: </strong>Mann-Whitney test, Chi-square, the Independent Student <i>t</i>-test, and one-way ANOVA test were used.</p><p><strong>Results: </strong>Salivary citrullinated proteins showed significant positive correlation with <i>Candida</i> (<i>P</i> = 0.003), <i>P. gingivalis</i> (<i>P</i> < 0.001), and DMFT scores (<i>P</i> = 0.001). RA patients demonstrated significantly higher colony-forming units of <i>Candida</i> (5.11 ± 0.50 vs. 2.13 ± 0.16 log₁₀; <i>P</i> < 0.001) and <i>P. gingivalis</i> (5.20 ± 0.08 vs. 2.12 ± 0.29 log₁₀; <i>P</i> < 0.001). CPI scores correlated with microbial load (<i>Candida</i>, <i>P</i> = 0.04; <i>P. gingivalis</i>, <i>P</i> = 0.009).</p><p><strong>Conclusions: </strong>Although not a standalone diagnostic marker, salivary citrullinated proteins correlate with microbial load and caries, supporting saliva-based biomarkers for RA monitoring.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"30 2","pages":"217-223"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148698094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of lymph node micrometastasis in oral squamous cell carcinoma - A retrospective histopathological study.","authors":"Kuntala Mondal, S V Sowmya, Dominic Augustine, Anam Tasneem","doi":"10.4103/jomfp.jomfp_122_25","DOIUrl":"10.4103/jomfp.jomfp_122_25","url":null,"abstract":"<p><strong>Context: </strong>Cervical lymph node metastasis is the most important prognostic factor in squamous cell carcinoma of head and neck. Lymph node prognostic determinants hold immense significance in influencing both patient's survival outcomes and the propensity for recurrence.</p><p><strong>Aims: </strong>To evaluate the diagnostic accuracy of Cyclin D1 and Pan-Cytokeratin (Pan-CK) for the detection of lymph node micrometastasis in oral squamous cell carcinoma (OSCC) tissue sections and re-assess the tumor staging.</p><p><strong>Settings and design: </strong>Study was executed at Department of Oral and Maxillofacial Pathology and Oral Microbiology, Faculty of Dental Sciences, M.S. Ramaiah University of Applied Sciences, Karnataka, Bangalore, India and study design was <i>In-vitro</i> type.</p><p><strong>Materials and methods: </strong>A total of 30 N₀ lymph node sections of OSCC cases were included in the study. Other than Hematoxylin and Eosin (H&E) and modified PAP (Papanicolaou), immunohistochemistry for Pan-CK and Cyclin D1 were used for identification of micrometastatic deposits.</p><p><strong>Results: </strong>Pan-CK and Cyclin D1 was helpful in detecting micrometastasis in 16.66% of N₀ lymph nodes which was statistically significant. Based on the results of immunobiomarkers, tumor staging was re-evaluated. Pan-CK and Cyclin D1 showed statistically significant detection of micrometastasis (<i>P</i> = 0.023), whereas Modified PAP did not reach statistical significance (<i>P</i> = 0.083).</p><p><strong>Conclusions: </strong>Pan-CK and Cyclin D1 were specific in detecting micrometastatic deposits compared H&E and PAP stains. Cyclin D1 immunostaining detects molecular overexpression in tumor cells missed on PAP or H&E, enabling identification of small lymph node deposits and improving staging accuracy and patient management.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"30 2","pages":"194-201"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148698118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary detection of viral DNA loads of HPV 16 and 18 in oral potentially malignant disorders and oral squamous cell carcinoma.","authors":"Haripriya Vaddalapu, Shravan Kumar, Moni Thakur, Kundoor V K Reddy","doi":"10.4103/jomfp.jomfp_78_26","DOIUrl":"10.4103/jomfp.jomfp_78_26","url":null,"abstract":"<p><strong>Introduction: </strong>Oral cancer is the sixth most common cancer in the world. It is a major cause of morbidity and mortality worldwide in developing countries. Viruses such as human papillomavirus (HPV) are known to play a substantial role in aetiopathogenesis of oral squamous cell carcinoma (OSCC).</p><p><strong>Aims and objectives: </strong>The aim of this study is to detect the viral DNA loads of HPV 16 and HPV 18 in the saliva samples collected from the oral potential malignant disorders (OPMDs) group, OSCC group, and control group; the objective of the present study is to analyse the possible role of HPV 16 and 18 in the pathogenesis of OSCC.</p><p><strong>Materials and methods: </strong>The study was carried out on 45 cases which were divided into three groups, \"A\", \"B\", and \"C\", comprising 15 cases each. Group \"A\" comprised disease-free subjects as controls. Group \"B\" comprised clinically and histopathologically diagnosed cases of OPMDs. Group \"C\" comprised OSCC. Results obtained were sent for statistical analysis. Chi-square test was performed to study the distribution of HPV 16 and HPV 18. Statistical analysis was performed using a computer with Statistical Package for Social Science software (SPSS VS 18). A <i>P</i> value of 0.005 was taken as standard.</p><p><strong>Results: </strong>Group \"A\" was found negative for both HPV 16 and 18; in group \"B\", out of 15 cases, 08 cases were positive for HPV 16 and 03 cases were positive for HPV 18, whereas in group \"C\", out of 15 cases, 10 cases were positive for HPV 16 and 04 cases were positive for HPV 18. The <i>P</i> value was 0.00 (<0.005) for HPV 16, which was significant, whereas for HPV 18, the <i>P</i> value was 0.111 (>0.005), which was insignificant.</p><p><strong>Conclusion: </strong>HPV 16 followed by HPV 18 may play a pivotal role in the pathogenesis of OPMDs and OSCC.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"30 2","pages":"224-228"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving diagnostic clarity in fibro-osseous lesions using modified Gallego's, alizarin red, and von kossa stains: A pilot comparative study.","authors":"Sharon Sam, Vijaya Nirmala Subramani, Anand Ramanathan, Thamizhchelvan Harikrishnan","doi":"10.4103/jomfp.jomfp_11_26","DOIUrl":"10.4103/jomfp.jomfp_11_26","url":null,"abstract":"<p><strong>Background: </strong>Distinguishing bone, cementum, and immature osteoid in maxillofacial fibro-osseous lesions (FOLs) can be challenging using routine haematoxylin and eosin (H&E) staining alone.</p><p><strong>Objective: </strong>To quantitatively evaluate the comparative diagnostic utility of H&E, Alizarin Red, Von Kossa, and Modified Gallego's stains (MGS) in identifying mineralized components across FOL subtypes.</p><p><strong>Materials and methods: </strong>In this pilot study, archival specimens of fibrous dysplasia (<i>n</i> = 2), cemento-osseous dysplasia (<i>n</i> = 2), cemento-ossifying fibroma (<i>n</i> = 2), and juvenile ossifying fibromas (<i>n</i> = 2) were stained. Independent pathologists scored structural feature visibility utilizing a semi-quantitative 0-3 scale. Visibility scores, inter-observer, and intra-observer reliability were analysed using Friedman, Wilcoxon signed-rank, and Gwet's AC1 tests.</p><p><strong>Results: </strong>Qualitatively, MGS provided consistent bone (green) versus cementum (red) differentiation, Alizarin Red isolated immature osteoid, and Von Kossa mapped mature, phosphate-rich calcifications. Quantitatively, inter-observer reliability demonstrated strong to perfect consensus, while intra-observer stability was moderate-to-perfect for MGS, slight-to-substantial for Alizarin Red, and poor-to-moderate for Von Kossa. Descriptively, MGS achieved the highest mean clarity score (2.93 ± 0.25), followed closely by Alizarin Red (2.80 ± 0.40) and Von Kossa (1.80 ± 1.17). Global Friedman and post-hoc pairwise comparisons confirmed that these differences were statistically significant (<i>P</i> = 0.0003), with both MGS (<i>P</i> = 0.0069) and Alizarin Red (<i>P</i> = 0.0057) yielding significantly superior diagnostic clarity over Von Kossa (<i>P</i> < 0.01).</p><p><strong>Conclusion: </strong>Integrating a targeted special stain selection protocol significantly overcomes the descriptive limitations of routine H&E, reducing diagnostic ambiguity in maxillofacial FOL configurations.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"30 2","pages":"303-311"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148698045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of clonogenic potential of primary cells from dental pulp and gingiva in an individual.","authors":"Raina Basu Roy, Emmanuel D S Azariah, C Deepak, Alan M Punnoose, Viswanathan Paramesh","doi":"10.4103/jomfp.jomfp_377_25","DOIUrl":"10.4103/jomfp.jomfp_377_25","url":null,"abstract":"<p><strong>Background: </strong>Regenerative medicine is an emerging field that combines biotechnology with aspects of medicine, cell and molecular biology, materials science and bioengineering to regenerate, repair or replace tissues. Since 1990, growth factors and platelet concentrates have been considered novel and have been widely used in oral and maxillofacial surgery. In the following years, biomaterials, as well as various types of scaffolds and autologous tissues, were increasingly used. Stem cells are defined as special cells that have the capacity to both self-renew and give rise to a cell population that can be differentiated.</p><p><strong>Aim: </strong>The aim of this study was to investigate and compare the yield of primary cells from dental pulp and gingival tissue. This study was designed to investigate the growth potential of dental pulp tissue and gingival tissue by assessing the colony-forming units.</p><p><strong>Materials and methods: </strong>Primary cells were isolated from dental pulp and gingival tissues collected from patients undergoing third molar extraction. Colony-forming unit (CFU) assay and scratch wound assay were performed to evaluate clonogenicity and proliferative potential.</p><p><strong>Results: </strong>Gingival stem cells demonstrated faster proliferation and higher colony-forming efficiency compared to dental pulp stem cells. Gingival stem cells also showed quicker wound closure in the scratch wound assay.</p><p><strong>Conclusion: </strong>Gingival tissue may serve as a more accessible and efficient source of stem cells for regenerative applications due to its rapid growth and enhanced clonogenic potential.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"30 2","pages":"280-286"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148698097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the role of salivary survivin and caspase-3 in apoptosis regulation in oral submucous fibrosis and oral squamous cell carcinoma among gutka chewers.","authors":"Arushi Chaure, Jaya Joshi, Sakshi Arora, Neha Choudhary, Supreet Kaur Gurdatta, Astha Pusame","doi":"10.4103/jomfp.jomfp_396_25","DOIUrl":"10.4103/jomfp.jomfp_396_25","url":null,"abstract":"<p><strong>Background: </strong>This study evaluates salivary Survivin and Caspase-3 as key regulators of apoptosis across a disease spectrum-from gutka chewers without lesions to oral submucous fibrosis (OSMF) and oral squamous cell carcinoma (OSCC). Survivin, an anti-apoptotic protein, and caspase-3, the principal executioner caspase, represent crucial biomarkers of malignant transformation, especially in populations with high smokeless tobacco exposure.</p><p><strong>Materials and methods: </strong>A cross-sectional analytical study included 92 participants divided into: controls (n = 17), gutka chewers without lesions (n = 25), OSMF (n = 25), and OSCC (n = 25). Unstimulated saliva samples were collected and analysed for Survivin and Caspase-3 using ELISA. Statistical analysis included Pearson correlation and Kruskal-Wallis.</p><p><strong>Results: </strong>Mean survivin levels demonstrated a progressive increase from healthy subjects (1.00 ± 0.73 pg/ml) to gutka chewers without lesions (2.00 ± 1.36 pg/ml), OSMF (3.50 ± 2.82 pg/ml), and OSCC (5.00 ± 3.04 pg/ml), indicating a trend towards increased anti-apoptotic activity with disease progression. In contrast, Caspase-3 levels showed a biphasic pattern, measuring 4.90 ± 4.43 ng/ml in healthy subjects, 4.50 ± 2.71 ng/ml in gutka chewers without lesions, decreasing to 3.50 ± 2.82 ng/ml in OSMF, and subsequently increasing to 5.50 ± 3.62 ng/ml in OSCC.</p><p><strong>Conclusion: </strong>Survivin demonstrated potential as a biomarker of malignant transformation in gutka-associated oral potentially malignant disorders, while the non-linear pattern of Caspase-3 highlights the complex regulatory interactions involving apoptosis suppression, hypoxic adaptation, and possible apoptosis-induced proliferation. However, both biomarkers may serve as potential indicators of disease progression, pending validation through larger multi-centre studies with longitudinal follow-up.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"30 2","pages":"312-319"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148698076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the anticancer effect of cytisine against oral cancer: Insights from bioinformatics and <i>in-vitro</i> approaches.","authors":"Savita S Thakkannavar, Deepa R Mane, Faizan Beerwala, Harish R Darasaguppe, Shahana Shahpuri","doi":"10.4103/jomfp.jomfp_86_26","DOIUrl":"10.4103/jomfp.jomfp_86_26","url":null,"abstract":"<p><strong>Introduction: </strong>Oral squamous cell carcinoma (OSCC), the most common malignancy, involves molecular alterations including p53 mutations, TGF-α, and EGFR overexpression. Targeting biomarkers is essential for effective therapy. Cytisine, a natural smoking-cessation alkaloid with diverse pharmacological activities, was evaluated using bioinformatics to identify potential anticancer targets and subsequently validated <i>in vitro</i>.</p><p><strong>Materials and methods: </strong>The in silico analysis included predictions of toxicity, drug-likeness, and potential targets for cytisine. OSCC-associated targets were retrieved from GeneCards and ACTREC databases. Cytoscape was used to construct cytisine-target pathways. Molecular docking and 100 ns molecular dynamics simulations were performed to evaluate cytisine binding affinity and structural stability to key targets. The <i>in vitro</i> validation was performed using the MTT assay and cell migration assay on an epithelial carcinoma cell model (KB-3-1 cell line).</p><p><strong>Results: </strong>Cytisine was non-toxic and modulated 23 target proteins and 13 signalling pathways. Gene enrichment analysis revealed significant interactions with EGFR and TP53. Molecular docking and 100 ns molecular dynamics simulation showed strong intermolecular interactions and low binding energies with EGFR (-6.9 kcal/mol) and TP53 (-7.7 kcal/mol) compared with standard drugs. <i>In vitro</i> assays demonstrated significant cytotoxicity and inhibition of cell migration in epithelial carcinoma cell lines while maintaining the viability of L929 normal fibroblast cells.</p><p><strong>Conclusion: </strong>Cytisine exhibits strong interactions and lower binding energies with EGFR and TP53, and cytotoxic and anti-migratory effects against the epithelial carcinoma cell model. However, further validation using primary oral squamous carcinoma cell lines and <i>in vivo</i> studies is necessary to confirm its therapeutic relevance to epithelial carcinoma.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"30 2","pages":"258-269"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic profiling of serum and saliva in rheumatoid arthritis: A pilot Q-TOF study.","authors":"Anitha Krishnan Pandarathodiyil, Ramya Ramadoss, K Hema Shree, Saranya Chinnadurai, B Sivapathasundharam","doi":"10.4103/jomfp.jomfp_111_26","DOIUrl":"10.4103/jomfp.jomfp_111_26","url":null,"abstract":"<p><strong>Background: </strong>Rheumatoid arthritis (RA) is a chronic autoimmune disease that primarily targets joints but also affects multiple organ systems. Current RA diagnostics rely on serum biomarkers like rheumatoid factor and anti-citrullinated protein antibodies, which indicate systemic inflammation but often lack sensitivity in the early stages. Recent advances in proteomics provide opportunities for discovering new biomarkers that could enhance diagnostic accuracy. Saliva, a non-invasive biofluid, has emerged as a potential alternative that may reflect both systemic and localized inflammation.</p><p><strong>Objective: </strong>This pilot study assesses whether saliva can complement serum in diagnosing and monitoring RA. Using quadrupole time-of-flight mass spectrometry, we compare serum and saliva proteomic profiles to identify RA-related biomarkers.</p><p><strong>Materials and methods: </strong>Serum and saliva samples from five RA patients and five healthy controls were analyzed using quadrupole time-of-flight mass spectrometry. Multivariate analyses, including partial least squares discriminant analysis and receiver operating characteristic analysis, were used to evaluate the diagnostic potential of saliva-based biomarkers relative to those in serum.</p><p><strong>Results: </strong>RA-related biomarkers, including citrullinated vimentin and tyrosine, were identified in both serum and saliva. Partial least squares discriminant analysis demonstrated distinct separation among sample groups, while receiver operating characteristic analysis indicated high diagnostic accuracy, supporting saliva's utility as a non-invasive complement to serum.</p><p><strong>Conclusion: </strong>Saliva shows promise as a complementary biofluid for RA diagnostics. It allows non-invasive sampling and the potential for frequent monitoring. While serum remains the standard for diagnosis, saliva could enhance disease monitoring in clinical practice.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"30 2","pages":"229-238"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of GLUT 1 expression in different grades of oral epithelial dysplasia and squamous cell carcinoma - An immunohistochemical study.","authors":"Neethu Kadar, Vishnudas Dinesh Prabhu, Maji Jose, Pm Mohamed Haris, Meera K Pynadath, Sushma Bommanavar","doi":"10.4103/jomfp.jomfp_263_25","DOIUrl":"10.4103/jomfp.jomfp_263_25","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Increased glucose uptake primarily mediated by Glucose Linked Transporter type 1 (GLUT1) is identified as one of the vital predictors of malignancies.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Aim and objective: &lt;/strong&gt;To compare and correlate GLUT1 expression in normal oral mucosa, different grades of oral epithelial dysplasia (OED) and oral squamous cell carcinomas (OSCCs).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Materials and methods: &lt;/strong&gt;The present retrospective study was conducted during the period of September 2023-August 2024 on a total of 45 (&lt;i&gt;n&lt;/i&gt; = 45) histologically graded formalin fixed paraffin embedded sections of OEDs, OSCC and normal oral mucosa that were immunostained with GLUT1. The samples were randomly divided into Study groups: Group 1 (OEDs), Group 2 (OSCC) and Control group (normal oral mucosa). Balanced equivalence sampling method was applied across all the groups with 15 samples each (&lt;i&gt;n&lt;/i&gt; = 15). Group 1 and Group 2 were further subdivided into subgroups as subgroup 1.1 (mild dysplasia), subgroup 1.2 (moderate dysplasia), subgroup 1.3 (severe dysplasia), subgroup 2.1 (well differentiated SCC), subgroup 2.1 (moderately differentiated SCC), and subgroup 2.3 (poorly differentiated SCC) with five samples each (&lt;i&gt;n&lt;/i&gt; = 5). Samples with membrane staining were classified as having a positive GLUT1 expression. Remmele and Stegner's immunoreactive score (IRS) was used to rate the staining intensity and percentage of positive cells. A bright field microscope was used to examine the slides, and photomicrographs were obtained to determine the proportion of positive cells. For semi-quantitative analysis, three regions (per case) with the highest GLUT1 expression were chosen at random. The mean value of the percentages was computed after the percentage of GLUT1 expression was measured. SPSS Version 19.0 was used for data analysis. Mean, frequencies, and percentages were used for categorical variables. The statistical analyses were done using Mann-Whitney and Kruskal Wallis Tests. A &lt;i&gt;P&lt;/i&gt; value &lt; 0.05 was considered statistically significant for all tests.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Group 1 had a total mean IRS score of 7.93 ± 2.219 with subgroup 1.1 (5.20 ± 1.095), subgroup 1.2 (9.00 ± 0.000) and subgroup 1.3 (9.60 ± 1.342), respectively, with highly significant &lt;i&gt;P&lt;/i&gt; value (&lt;i&gt;P&lt;/i&gt; &lt; 0.001). Group 2 had a total mean IRS score of 9.40 ± 2.230 with subgroup 2.1 (7.20 ± 1.643), subgroup 2.2 (9.00 ± 0.000) and subgroup 2.3 (12.00 ± 0.000) with highly significant &lt;i&gt;P&lt;/i&gt; value (&lt;i&gt;P&lt;/i&gt; &lt; 0.001). However, the control group reported the least total mean IRS score of 2.47 ± 1.407 (&lt;i&gt;P&lt;/i&gt; &gt; 0.05). However, there was no significant difference in the Mean IRS between Group 1 (epithelial dysplasia) and Group 2 (OSCC) (&lt;i&gt;P&lt;/i&gt; = 0.150). When the mean IRS differences between Group 1 (epithelial dysplasia) and normal control, as well as between Group 2 (OSCC) and normal control, were compared, the results were determined to be highly si","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"30 2","pages":"295-302"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148698115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}