Comparative analysis of periodontal ligament stem cell characteristics in type 2 diabetic and non-diabetic patients with periodontitis.

Q3 Medicine
Sudarvizhi Thiruvasagam, Muhammad Nihad, Shruthi Nayak, Debajit Chaudhury, Maji Jose, P Sudheer Shenoy
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Abstract

Context: Periodontitis, whether associated with diabetes or not, may have an impact on periodontal ligament stem cells (PDLSCs), which can adversely affect the regeneration of lost periodontal tissues and therefore treatment outcome. This study aimed to analyse the characteristics and differentiation potential of PDLSCs isolated from periodontitis patients with and without diabetes.

Aims: To understand the effect of diabetes mellitus (type II) on the periodontal ligament cells in patients with periodontitis.

Settings and design: A cross-sectional prospective study was carried out from the Yenepoya Research Centre and from the Department of Periodontology, Yenepoya Dental College for sample collection.

Methods and material: The study utilized cell culture media, antibodies, an MTT assay kit and a differentiation kit. PDLSCs were isolated from granulation tissues of diabetic and non-diabetic periodontitis patients. Immunophenotyping confirmed mesenchymal markers, and the cells were evaluated for viability, migration, proliferation and differentiation into osteogenic, chondrogenic and adipogenic lineages.

Statistical analysis used: Data analysed using SPSS version 22. The Kruskal-Wallis H test was used, with statistical significance set at P < 0.05.

Results: PDLSCs isolated from all three sources exhibited similar phenotypic characteristics, including high expression of mesenchymal markers and showed comparable cell growth, viability, proliferation and migration (P < 0.05). All groups demonstrated chondrogenic and osteogenic differentiation, with no evidence of adipogenic potential, indicating that even inflamed tissue can yield functional PDLSCs.

Conclusion: PDLSCs can be successfully isolated from inflamed periodontal granulation tissue in both diabetic and non-diabetic patients, exhibiting similar phenotypic characteristics to healthy PDLSCs while retaining comparable proliferative, migratory and differentiation potential.

2型糖尿病与非糖尿病牙周炎患者牙周韧带干细胞特征的比较分析。
背景:牙周炎,无论是否与糖尿病相关,都可能对牙周韧带干细胞(PDLSCs)产生影响,这可能对失去的牙周组织的再生产生不利影响,从而影响治疗结果。本研究旨在分析伴有和不伴有糖尿病的牙周炎患者分离的PDLSCs的特征和分化潜力。目的:了解糖尿病(II型)对牙周炎患者牙周韧带细胞的影响。环境和设计:一项横断面前瞻性研究从叶内波亚研究中心和叶内波亚牙科学院牙周病学系进行样本收集。方法和材料:采用细胞培养基、抗体、MTT测定试剂盒和分化试剂盒。从糖尿病和非糖尿病牙周炎患者肉芽组织中分离PDLSCs。免疫表型分析证实了间充质标志物,并评估了细胞的活力、迁移、增殖和向成骨、软骨和脂肪谱系的分化。使用的统计分析:数据分析使用SPSS版本22。采用Kruskal-Wallis H检验,差异有统计学意义,P < 0.05。结果:从三种来源分离的PDLSCs均表现出相似的表型特征,包括间充质标志物的高表达,细胞生长、活力、增殖和迁移表现相似(P < 0.05)。所有组均表现出成软骨和成骨分化,没有证据表明存在成脂肪潜能,这表明即使是炎症组织也能产生功能性PDLSCs。结论:糖尿病和非糖尿病患者均可成功从炎症性牙周肉芽组织中分离出PDLSCs,其表型特征与健康的PDLSCs相似,同时具有相当的增殖、迁移和分化潜力。
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来源期刊
Journal of Oral and Maxillofacial Pathology
Journal of Oral and Maxillofacial Pathology Medicine-Otorhinolaryngology
CiteScore
1.40
自引率
0.00%
发文量
115
期刊介绍: The journal of Oral and Maxillofacial Pathology [ISSN:print-(0973-029X, online-1998-393X)] is a tri-annual journal published on behalf of “The Indian Association of Oral and Maxillofacial Pathologists” (IAOMP). The publication of JOMFP was started in the year 1993. The journal publishes papers on a wide spectrum of topics associated with the scope of Oral and Maxillofacial Pathology, also, ensuring scientific merit and quality. It is a comprehensive reading material for the professionals who want to upgrade their diagnostic skills in Oral Diseases; allows exposure to newer topics and methods of research in the Oral-facial Tissues and Pathology. New features allow an open minded thinking and approach to various pathologies. It also encourages authors to showcase quality work done by them and to compile relevant cases which are diagnostically challenging. The Journal takes pride in maintaining the quality of articles and photomicrographs.
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