Sudarvizhi Thiruvasagam, Muhammad Nihad, Shruthi Nayak, Debajit Chaudhury, Maji Jose, P Sudheer Shenoy
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引用次数: 0
Abstract
Context: Periodontitis, whether associated with diabetes or not, may have an impact on periodontal ligament stem cells (PDLSCs), which can adversely affect the regeneration of lost periodontal tissues and therefore treatment outcome. This study aimed to analyse the characteristics and differentiation potential of PDLSCs isolated from periodontitis patients with and without diabetes.
Aims: To understand the effect of diabetes mellitus (type II) on the periodontal ligament cells in patients with periodontitis.
Settings and design: A cross-sectional prospective study was carried out from the Yenepoya Research Centre and from the Department of Periodontology, Yenepoya Dental College for sample collection.
Methods and material: The study utilized cell culture media, antibodies, an MTT assay kit and a differentiation kit. PDLSCs were isolated from granulation tissues of diabetic and non-diabetic periodontitis patients. Immunophenotyping confirmed mesenchymal markers, and the cells were evaluated for viability, migration, proliferation and differentiation into osteogenic, chondrogenic and adipogenic lineages.
Statistical analysis used: Data analysed using SPSS version 22. The Kruskal-Wallis H test was used, with statistical significance set at P < 0.05.
Results: PDLSCs isolated from all three sources exhibited similar phenotypic characteristics, including high expression of mesenchymal markers and showed comparable cell growth, viability, proliferation and migration (P < 0.05). All groups demonstrated chondrogenic and osteogenic differentiation, with no evidence of adipogenic potential, indicating that even inflamed tissue can yield functional PDLSCs.
Conclusion: PDLSCs can be successfully isolated from inflamed periodontal granulation tissue in both diabetic and non-diabetic patients, exhibiting similar phenotypic characteristics to healthy PDLSCs while retaining comparable proliferative, migratory and differentiation potential.
期刊介绍:
The journal of Oral and Maxillofacial Pathology [ISSN:print-(0973-029X, online-1998-393X)] is a tri-annual journal published on behalf of “The Indian Association of Oral and Maxillofacial Pathologists” (IAOMP). The publication of JOMFP was started in the year 1993. The journal publishes papers on a wide spectrum of topics associated with the scope of Oral and Maxillofacial Pathology, also, ensuring scientific merit and quality. It is a comprehensive reading material for the professionals who want to upgrade their diagnostic skills in Oral Diseases; allows exposure to newer topics and methods of research in the Oral-facial Tissues and Pathology. New features allow an open minded thinking and approach to various pathologies. It also encourages authors to showcase quality work done by them and to compile relevant cases which are diagnostically challenging. The Journal takes pride in maintaining the quality of articles and photomicrographs.