Daffney Mano Doss, Ramdas Madhava Nirmal, Veeravarmal Veeran, R Saravanan, J Sridevi, J Beryl Rachel
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引用次数: 0
Abstract
Aim: The aim of the present study is to analyse the role of glucose transporter (GLUT)-1 in oral cancer. This study was performed to evaluate the expression of GLUT-1 in normal oral epithelium, to evaluate the expression of GLUT-1 levels in the tissue samples of grades of oral squamous cell carcinoma (OSCC) [well-differentiated (WD), moderately differentiated (MD), and poor differentiated (PD)], and to statistically compare the expression of GLUT-1 in normal epithelium and in the grades of OSCC.
Materials and methods: The study sample comprised formalin-fixed and paraffin-embedded tissue specimens from 12 cases of histopathologically diagnosed WD OSCC and ten cases of MD and PD OSCC and formalin-fixed paraffin-embedded tissue specimens from ten cases of normal oral mucosa. Sections were mounted on glass slides coated with aminopropyltriethoxysilane (APES; Sigma chemical co., USA) and processed for subsequent immunohistochemical study to demonstrate GLUT-1.
Results: In our study, the GLUT-1 expression score of OSCC demonstrated strong positivity in 16 cases (50%), weak positivity in 11 cases (34.38%), and negativity in 5 cases (15.62%). There was significant correlation at a P value of 0.007 for expression of GLUT-1 in normal oral epithelium and OSCC.
Conclusion: Over-expression of GLUT-1 in peripheral cells of tumour islands and invasive front could reflect the active metabolism which may be taking place in these areas where cellular proliferation and invasion is at the highest. The notable expression of GLUT-1 in malignant cells reveals glucose transporters play a key role in tumour progression and metastasis.
目的：本研究的目的是分析葡萄糖转运蛋白(GLUT)-1在口腔癌中的作用。本研究旨在评估GLUT-1在正常口腔上皮中的表达情况，评估GLUT-1在口腔鳞状细胞癌（OSCC）分级[高分化（WD）、中分化（MD）和低分化（PD）]组织样本中的表达水平，并对正常上皮和OSCC分级中GLUT-1的表达进行统计学比较。材料与方法：研究样本为取自12例经组织病理学诊断为WD型OSCC的福尔马林固定石蜡包埋组织标本，以及取自10例正常口腔黏膜的MD、PD型OSCC和福尔马林固定石蜡包埋组织标本。切片装在涂有氨基丙基三乙基氧基硅烷（APES）的玻片上；Sigma chemical co.， USA)，并进行后续免疫组织化学研究以证实GLUT-1。结果：本研究中，GLUT-1在OSCC中的表达评分为强阳性16例（50%），弱阳性11例（34.38%），阴性5例（15.62%）。正常口腔上皮中GLUT-1的表达与OSCC呈显著相关，P值为0.007。结论：GLUT-1在肿瘤岛外周细胞和侵袭前缘的高表达，反映了肿瘤岛外周细胞和侵袭前沿细胞可能发生了活跃的代谢活动。GLUT-1在恶性肿瘤细胞中的显著表达表明葡萄糖转运蛋白在肿瘤进展和转移中起关键作用。
期刊介绍：
The journal of Oral and Maxillofacial Pathology [ISSN:print-(0973-029X, online-1998-393X)] is a tri-annual journal published on behalf of “The Indian Association of Oral and Maxillofacial Pathologists” (IAOMP). The publication of JOMFP was started in the year 1993. The journal publishes papers on a wide spectrum of topics associated with the scope of Oral and Maxillofacial Pathology, also, ensuring scientific merit and quality. It is a comprehensive reading material for the professionals who want to upgrade their diagnostic skills in Oral Diseases; allows exposure to newer topics and methods of research in the Oral-facial Tissues and Pathology. New features allow an open minded thinking and approach to various pathologies. It also encourages authors to showcase quality work done by them and to compile relevant cases which are diagnostically challenging. The Journal takes pride in maintaining the quality of articles and photomicrographs.
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