Plant Gene最新文献

{"title":"Induced genetic diversity through EMS mutagenesis in Davana (Artemisia pallens Bess.) and use of ISSR markers to underpin agronomical and chemical traits","authors":"Arun J. Ganiger ,&nbsp;Channayya Hiremath ,&nbsp;K. Madhusudan ,&nbsp;Manoj kumar Chandrasekaran ,&nbsp;V.S. Pragadheesh","doi":"10.1016/j.plgene.2025.100543","DOIUrl":"10.1016/j.plgene.2025.100543","url":null,"abstract":"<div><div>Davana (<em>Artemisia pallens</em> Bess.) is an annual aromatic herb that belongs to the <em>Asteraceae</em> family. The current research was attempted to identify elite high essential oil yielding davana mutants with high davanone content. To determine the LD₅₀ value of EMS, seeds of the local davana cultivar “Bangalore” were treated with the chemical mutagen ethyl methane sulphonate (EMS) at varying concentrations of 0.10 %, 0.2 %, 0.3 %, 0.40 %, and 0.50 % for 16 h. The LD₅₀ value was (0.40 % for 16 h) determined by probit analysis. In the M₁ generation, 3000 davana plants were evaluated and forwarded to M₂. Based on visual observation of 1500 M₂ plants, 250 mutants were chosen, and their essential oil and davanone content was assessed. In the M₂ generation, the quantitative traits evaluated and chemical constituents assessed had higher mean and moderate to high phenotypic co-efficient of variation, in comparison to the control. Essential oil yield had a strong and significantly positive correlation to the number of flowers per plant, flower head diameter, and fresh herb yield traits. Based on superiority over control and Mean + 1 SD of M₂ population, 22 and 14 mutants were identified for essential oil yield and davanone content, respectively. Molecular diversity analysis of 16 high essential oil yielding M₂ plants and two check cultivars using ISSR markers revealed an average 79 % polymorphism, with the primer UBC-845 having the highest polymorphic information content. We identified several superior mutants including DM2158 (essential oil content-0.5 %) and DM2103 (Davanone content-70.92 %), indicating that mutation breeding can be an effective strategy to develop novel davana cultivars with higher essential oil and davanone content.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"44 ","pages":"Article 100543"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal and spatial expression analysis of AtbZIP9 during seed and silique development in Arabidopsis thaliana (L.) Heynh","authors":"Jonatan Illescas-Miranda ,&nbsp;Victoria Llanos-Casado ,&nbsp;Estefanía Contreras,&nbsp;Néstor Carrillo-Barral,&nbsp;Raquel Iglesias-Fernández","doi":"10.1016/j.plgene.2025.100536","DOIUrl":"10.1016/j.plgene.2025.100536","url":null,"abstract":"<div><div>In <em>Arabidopsis thaliana</em>, seed dispersal is mediated by the silique, a specialized fruit that undergoes a complex developmental program involving cell division, expansion, and programmed cell death. Transcription factors (TFs) from the bZIP family are key regulators of these transitions. In this study, we focused on the C-group bZIP TF AtbZIP9 to characterize its expression, potential regulatory roles, and functional relevance during silique development and early seedling growth. Promoter-reporter assays and qPCR analyses revealed that AtbZIP9 is broadly expressed, with strong activity in vascular tissues and the funiculus during early and mid-stages of silique development. AtbZIP9 physically interacts with the S1-group member AtbZIP44, as shown by yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays, supporting the formation of heterodimeric complexes. Despite the lack of major phenotypic alterations in <em>AtbZIP9</em> knockout mutants during germination and early development—even under salt stress conditions—its co-expression with <em>AtbZIP44</em> and the CW-modifying gene <em>AtMAN7</em> suggests a role in transcriptional regulation during silique development. Recent evidence further links AtbZIP9 to ABA-responsive gene expression and identifies it as a likely component of redundant regulatory networks involving other C-group bZIPs. These findings highlight AtbZIP9 as a candidate transcriptional modulator of silique and seed developmental processes, potentially acting in coordination with AtbZIP44 and other factors.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"44 ","pages":"Article 100536"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144841494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning and functional characterization of three cinnamate 4-hydroxylase isoforms from Marchantia polymorpha associated with UV-absorbing compounds production","authors":"Jiayi Yang,&nbsp;Jian Mo,&nbsp;Baoyun Shan,&nbsp;Xiaochun Qin,&nbsp;Haina Yu","doi":"10.1016/j.plgene.2025.100558","DOIUrl":"10.1016/j.plgene.2025.100558","url":null,"abstract":"<div><div>Cinnamate 4-hydroxylase (C4H) catalyzes the second step of the phenylpropanoid pathway to generate precursor for phenolic compounds. As phenylpropanoid-derived specialized metabolites, Ultraviolet (UV)-absorbing compounds (UVACs) exhibit distinct UV-B absorption. In this study, we cloned and functionally characterized three C4H homologs (MpCYP73A1, A2 and A3) from the liverwort <em>Marchantia polymorpha.</em> Bioinformatic analyses confirmed that all MpCYP73 isoforms maintain conserved cytochrome P450 domains characteristic of plant C4H enzymes. Heterologous expression of recombinant MpCYP73 isoforms in yeast microsomes confirmed their functional identity as cinnamate 4-hydroxylases (C4H, EC 1.14.14.91). The enzymes catalyzed <em>trans</em>-cinnamic acid and 3-hydroxycinnamic acid into <em>p</em>-coumaric acid and caffeic acid, respectively. The three MpCYP73 isoforms exhibited distinct kinetic profiles, with MpCYP73A1 displaying the lowest <em>K</em>_<em>m</em> value for <em>trans</em>-cinnamic acid and MpCYP73A3 showing the highest affinity for 3-hydroxycinnamic acid. Furthermore, their catalytic activities toward both substrates also demonstrated marked variation. In addition, <em>MpCYP73A1</em> displayed the highest transcript abundance, positively correlating with UVACs accumulation. Furthermore, UV-B treatment significantly induced the expression of <em>MpCYP73s</em> and enhanced the total content of methanol-soluble UVACs. These findings provide a basis for further investigation on the critical regulatory role of MpCYP73 isoforms in UVACs biosynthesis in <em>M. polymorpha</em>.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"44 ","pages":"Article 100558"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145465723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive genome-wide study of Glutaredoxin (GRX) gene family in the liverwort Marchantia polymorpha and exploring their roles in abiotic stress tolerance","authors":"Shivani Singh ,&nbsp;Garima Saxena ,&nbsp;Prachi Mishra ,&nbsp;Monica Kumari ,&nbsp;Prasanna Dutta ,&nbsp;Mehar Hasan Asif ,&nbsp;Debasis Chakrabarty","doi":"10.1016/j.plgene.2025.100550","DOIUrl":"10.1016/j.plgene.2025.100550","url":null,"abstract":"<div><div>Glutaredoxins (GRXs) are thiol-disulfide oxidoreductases that function as key regulators of redox homeostasis, development, and stress responses in plants. Despite their functional importance, little is known about the GRX gene family in early diverging land plants. In this study, we performed a comprehensive genome-wide identification of GRX genes in the liverwort <em>Marchantia polymorpha</em> and their role in abiotic stresses. A total of 17 <em>MpGRX</em> genes with 2 isoforms (19 <em>MpGRX</em>) containing the conserved Glutaredoxin domain (PF00462) were identified using BLAST, HMMER, and SMART approaches. Gene structure analysis revealed that six <em>MpGRX</em> genes contained a single intron, whereas the remaining genes exhibited more complex structures with three or more introns, suggesting gene expansion and functional diversification. Evolutionary analysis was conducted using non-synonymous (Ka) and synonymous (Ks) substitution rates between <em>MpGRX</em> genes and homologous genes from <em>Physcomitrella patens</em>, <em>Ceratopteris richardii</em>, <em>Pinus taeda</em>, <em>Arabidopsis thaliana</em>, and <em>Oryza sativa</em>. The results indicated generally low Ka values, except in one <em>MpGRX–PtGRX</em> pair with Ka = 1.59, suggesting potential functional divergence in gymnosperms. Ks-based divergence time estimates were consistent with known evolutionary separations. Selection pressure analysis based on Ka/Ks ratios revealed that most GRX gene pairs were under purifying selection, particularly those between <em>M. polymorpha</em> and mosses, ferns, and angiosperms.</div><div>This study provides novel insights into the structural diversity, evolutionary history, and selective constraints acting on the GRX gene family in <em>M. polymorpha</em>, offering a foundation for future functional and comparative studies in early land plant lineages.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"44 ","pages":"Article 100550"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene expression analysis of four Solanum tuberosum L. varieties in vitro induced with Alternaria alternata and Streptomyces werraensis extracts","authors":"Izdihar Ferhat ,&nbsp;Wahiba Harrat ,&nbsp;Emre Yörük ,&nbsp;Boualem Harfi","doi":"10.1016/j.plgene.2025.100546","DOIUrl":"10.1016/j.plgene.2025.100546","url":null,"abstract":"<div><div>Potato (<em>Solanum tuberosum</em> L.) is one of the major tuber food crops worldwide. However, its culture is threatened by several diseases like potato brown leaf spot which is related to <em>Alternaria</em> fungi. This study addresses the challenge of inducing resistance in four potato varieties against brown spot disease by <em>in vitro</em> culture. The approach relies on combining <em>Streptomyces werraensis</em> extracts, reported as potential biocontrol agents, and <em>Alternaria alternata</em> pathogen extracts in the potato culture medium. Initially, potato micropropagation and microtuberization media were optimized, followed by an acclimatization phase and a pathogenicity test on the four varieties (Arizona, Désirée, Spunta and Synergy). Subsequently, potato microtubers were produced <em>in vitro</em>, with <em>microorganisms'</em> extracts included in the culture medium. To assess for induced resistance, qRT-PCR analysis was conducted to evaluate gene expression levels, targeting the most relevant genes (<em>ChtA1</em>, <em>ChtB3</em>, <em>PR1b</em>, <em>PRa1-like</em>, <em>WRKY31</em> and <em>WRKY75-like</em>). Gene expression analysis revealed significant differences between varieties, with Arizona variety showing the most vigorous response. These distinct varietal responses indicate genotype-specific reactions to the combined treatment of <em>A. alternata</em> and <em>S. werraensis</em> extracts. This research highlights the <em>S. werraensis</em> potential as a biocontrol agent to induce resistance in potatoes to <em>A. alternata</em>. The results support the integration of <em>in vitro</em> culture media supplemented with microorganism extracts for large-scale standardized potato production.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"44 ","pages":"Article 100546"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative leaf transcriptomic and physiological insights into salt stress responses in Eucalyptus: A comparative analysis of two contrasting clones","authors":"Ravita Tadiya ,&nbsp;Santan Barthwal ,&nbsp;Harish Singh Ginwal ,&nbsp;Hukum Singh ,&nbsp;Fateh Singh","doi":"10.1016/j.plgene.2025.100551","DOIUrl":"10.1016/j.plgene.2025.100551","url":null,"abstract":"<div><div>Salinity severely limits forest productivity; however, the molecular basis of tolerance in <em>Eucalyptus</em> remains unclear. We investigated the physiological and transcriptomic responses of a salt-tolerant clone (B-112) and a salt-sensitive clone (W-12) subjected to 80 days of severe salinity (EC ≥ 20 dS/m). Both clones showed reductions in photosynthesis, stomatal conductance, internal CO₂ concentration, and transpiration; however, the extent of decline was considerably smaller in B-112, indicating superior physiological resilience. RNA-seq profiling revealed the targeted reprogramming of 1025 genes in B-112, compared with a broad, less coordinated response involving 14,994 genes in W-12. In B-112, enrichment analyses highlighted the strong activation of abscisic acid (ABA) and ethylene signaling, mitogen-activated protein kinases (MAPKs) cascades, carbohydrate metabolism, antioxidant defense, and protein folding pathways. Key salt-responsive genes included Dehydrins (DHN1), Ethylene Response Factors (ERFs), cupredoxins, and expansins, indicating coordinated osmotic adjustment, cell-wall modification, and stress signaling. Conversely, W-12 displayed extensive upregulation of ubiquitination and vesicle trafficking genes, alongside repression of photosynthesis and chloroplast-related processes, consistent with a damage-induced rather than adaptive program. qRTPCR validation confirmed high concordance with RNAseq results. Together, these findings reveal that B-112 sustains salt tolerance through proactive and focused transcriptomic reprogramming, while W-12 exhibits reactive stress-triggered responses, providing candidate targets for breeding and genetic improvement of <em>Eucalyptus</em> in saline environments.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"44 ","pages":"Article 100551"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145157959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of the Delay of Germination (DoG1) gene and ethrel mediated regulation of seed dormancy in peanut","authors":"Chandramohan Sangh,&nbsp;Swati Kasundra,&nbsp;Praveen Kona,&nbsp;Radhakrishnan Thankappan,&nbsp;Sandip Kumar Bera","doi":"10.1016/j.plgene.2025.100555","DOIUrl":"10.1016/j.plgene.2025.100555","url":null,"abstract":"<div><div>The <em>DoG1</em> (Delay of Germination 1) gene family has emerged as an important regulator in the post-genomic era owing to its critical roles in seed dormancy, plant growth, and development. Although extensively studied in several crops, its characterization in peanut (<em>Arachis hypogaea</em>) has not yet been undertaken. To address this gap, the present study aimed to identify <em>DoG1</em>-like (<em>AhDoG1L</em>) genes in peanut and investigate structural features, expression, and evolutionary relationships. Using a peanut-specific HMM, we identified 34 <em>AhDoG1L</em> genes, all of which contained a conserved DoG1 domain, with some also harboring a bZIP domain. Protein sequence analysis predicted nuclear localization, and phylogenetic classification grouped these genes into three subclades, with tandem duplications preferentially distributed at chromosome termini. Comparative synteny and orthology analyses with soybean and sunflower revealed conserved evolutionary relationships. Promoter analysis identified 10 conserved motifs and multiple cis-acting regulatory elements responsive to light, hormones, and low temperature, suggesting diverse regulatory mechanisms. Functional annotation through GO enrichment and protein–protein interaction networks further supported roles in transcriptional regulation and dormancy control. Expression analysis using qRT-PCR demonstrated that 11 <em>AhDoG1L</em> genes (<em>p</em> &lt; 0.05) showed significantly higher expression in freshly harvested seeds than in germinated seeds, indicating a role in seed dormancy in the TG37 genotype. Moreover, ethrel treatment in PBS 15014 led to the identification of <em>AhDoG1</em>-<em>L34</em>, <em>AhDoG1</em>-<em>L30</em>, and <em>AhDoG1</em>-<em>L22</em> (<em>p</em> &lt; 0.05), highlighting responsiveness to ethrel. Collectively, these findings provide the first comprehensive overview of the <em>AhDoG1</em> gene family in peanut, offering insights into their potential involvement in regulating seed dormancy and germination.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"44 ","pages":"Article 100555"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145415854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induced mutagenesis confers earliness and salt tolerance in ricebean (Vigna umbellata)","authors":"Soumik Dey ,&nbsp;Aditya Pratap Singh ,&nbsp;Sutanu Sarkar ,&nbsp;Kalyan Jana ,&nbsp;Mojtaba Kordrostami ,&nbsp;Mehdi Rahimi","doi":"10.1016/j.plgene.2025.100556","DOIUrl":"10.1016/j.plgene.2025.100556","url":null,"abstract":"<div><div>Ricebean (<em>Vigna umbellata</em> [Thunb.] Ohwi and Ohashi) is an underutilized legume valued for its dual role as a protein-rich pulse and high-quality forage crop. However, its commercial adoption is limited due to late maturity and susceptibility to salinity stress. Eleven M5 mutant lines derived from gamma-irradiated seeds of the late-maturing variety BRB-1 were evaluated under single-site field trials and controlled salinity assays. Several mutants flowered and matured ∼2 weeks earlier than BRB-1 and showed higher mean forage protein and seed/forage yield under these single-site trials. One line displayed superior germination and seedling performance under in-vitro salinity screening. These results indicate that induced mutagenesis can generate promising early-maturing and salt-tolerant candidates; however, multi-location and multi-season evaluations and physiological/molecular validation are required before recommending release or broad dual-purpose deployment.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"44 ","pages":"Article 100556"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145415857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the genetic diversity of common bean germplasm: insights into Andean gene pool variability","authors":"Cecilia Luvizutti Ferreira Silva ,&nbsp;Dario Grattapaglia ,&nbsp;Paula Arielle Mendes Ribeiro Valdisser ,&nbsp;Paula Pereira Torga ,&nbsp;Alessandra da Cunha Moraes Rangel ,&nbsp;Claudio Brondani ,&nbsp;Alexandre Siqueira Guedes Coelho ,&nbsp;Tereza Cristina de Oliveira Borba ,&nbsp;Rosana Pereira Vianello","doi":"10.1016/j.plgene.2025.100540","DOIUrl":"10.1016/j.plgene.2025.100540","url":null,"abstract":"<div><div>Common bean (<em>Phaseolus vulgaris</em>) is among the most widely consumed legumes globally, with Brazil playing a crucial role in preserving and expanding its existing genetic diversity. This study aimed to characterize a subset of 863 common bean accessions from Brazil's Gene Banks, mainly of Andean origin. A germplasm collection was genotyped with 4275 SNPs using the EMBRAPA Multispecies 65KChip. Population structure analysis revealed two main groups: Andean (<em>n</em> = 558) and Middle American (<em>n</em> = 267), along with 38 admixed accessions. Andean group exhibited lower gene diversity (GD = 0.086), and allelic richness (A_R = 1.83) compared to the Middle American group (GD = 0.270, A_R = 1.98). Brazilian landraces in the Andean and Middle American groups showed potentially lower overall diversity, emphasizing the need for conservation efforts to preserve these genetic resources. The Andean Brazilian core collection (<em>n</em> = 221; GD = 0.095) encompasses the active collection diversity evaluated (<em>n</em> = 337; GD = 0.093). A total of 26 SNPs potentially under selection, mainly associated with plant development and defense, were identified. Georeferencing landraces using climate maps identified potentially valuable varieties adapted to drought (e.g., BGF0011779, BGF0012528, BGF0013826) and high temperatures and low-fertility soils (e.g., BGF0016128, BGF0013871), highlighting their relevance for conservation and sustainable use in breeding programs. Accessions' photographs showcased a wide range of morphological diversity, colors, types, shapes, and sizes of beans. Our findings reveal a significant genetic diversity among common bean germplasm, offering practical breeding opportunities and enhancing the value of gene bank collections. Certain landraces show potential for adapting to challenging climatic conditions, making them promising subjects for further adaptation studies.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"44 ","pages":"Article 100540"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of the CONSTANS-like gene family and its expression profiling under salt treatment in alfalfa (Medicago sativa L.)","authors":"Shuzhi Ma,&nbsp;Yiping Wei,&nbsp;Qinyan Bao,&nbsp;Zhaozhu Wen,&nbsp;Mengli Luo,&nbsp;Wenxuan Du","doi":"10.1016/j.plgene.2025.100544","DOIUrl":"10.1016/j.plgene.2025.100544","url":null,"abstract":"<div><div>Alfalfa (<em>Medicago sativa</em> L.) is a globally cultivated, high-quality forage crop. The CONSTANS-LIKE (COL) genes play crucial roles in regulating flowering time and stress responses in plants. However, the functional characterization of <em>COL</em> genes in alfalfa remain largely unexplored. In this study, we conducted a genome-wide analysis of the Xinjiang Daye alfalfa genome and identified 36 <em>MsCOL</em> genes. Multiple sequence alignment confirmed that all <em>MsCOL</em> genes contain conserved B-box and CCT (CO, CO-like and TOC1) domains. Phylogenetic analysis and gene structure analyses classified the <em>MsCOL</em> genes into two subclades, exhibiting similar exon-intron organization and conserved motif distributions. Chromosomal mapping revealed that these genes are distributed across 22 chromosomes. Expression profiling under salt stress demonstrated that <em>MsCOL</em> genes exhibit expression patterns, indicating their potential involvement in abiotic stress responses. These findings enhance our understanding of <em>MsCOL</em> gene function in alfalfa and provide a theoretical basis for breeding salt-tolerant alfalfa cultivars.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"44 ","pages":"Article 100544"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144920283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}