Plant Gene最新文献

{"title":"Structural and functional insights into Jasmonate ZIM-domain 2 (GhJAZ2) interactions in cotton fiber development via Jasmonic acid signaling","authors":"Muhammad Sulyman Saleem ,&nbsp;Sultan Habibullah Khan ,&nbsp;Zunaira Afzal Naveed","doi":"10.1016/j.plgene.2025.100528","DOIUrl":"10.1016/j.plgene.2025.100528","url":null,"abstract":"<div><div>Cotton is one of the world's most vital fiber crops, and enhancing its fiber quality continues to be a major priority for the textile sector. The protein GhJAZ2, belonging to the Jasmonate ZIM-domain (JAZ) family, functions as a transcriptional repressor that influences fiber initiation by interacting with proteins such as GhMYB25-like, GhWD40, GhMYC2, GhGL1, and GhJI1, all of which participate in jasmonic acid (JA) signaling pathways. Despite their known involvement, detailed insights into their interactions and precise three-dimensional structures remain elusive.</div><div>In this research, we aimed to characterize the functional roles of GhJAZ2 and its interacting partners, and to model their structural interactions computationally. We utilized homology-based modeling to construct 3D models and applied HADDOCK software for protein-protein docking to accurately pinpoint interaction sites and assess binding affinities. Our findings indicated that the ZIM domain of GhJAZ2 serves as the central interaction region. Specifically, key residues within the TIFY motif, particularly ILE126 and TYR128, play a significant role in binding GhWD40, GhMYC2, GhGL1, and GhJI1 proteins. These results underscore the critical role of the TIFY motif in enabling GhJAZ2 to act as a repressor during fiber development.</div><div>This study provides valuable new insights into the molecular processes involved in JA-driven fiber development in cotton, identifying potential genetic targets that could be manipulated to significantly improve fiber quality.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100528"},"PeriodicalIF":2.2,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of HD-Zip transcription factor family in Artemisia argyi and functional analysis of AaHDZ64 in trichome development","authors":"Zhanhu Cui ,&nbsp;Chunyan Miao ,&nbsp;Heyang Shang ,&nbsp;Qian Zhao ,&nbsp;Shujiao Li ,&nbsp;Zhongyi Zhang ,&nbsp;Yuqing Wang ,&nbsp;Xianzhang Huang","doi":"10.1016/j.plgene.2025.100529","DOIUrl":"10.1016/j.plgene.2025.100529","url":null,"abstract":"<div><div>The homeodomain-leucine zipper (HD-Zip) family of transcription factor is a unique and vital gene family in plants, involved in various processes including organ development, stress responses, and regulation of secondary metabolite biosynthesis. This study aimed to investigate the role of HD-Zip family transcription factors in the development of <em>Artemisia argyi</em> trichomes. By utilizing the whole genome data of <em>A. argyi</em>, we identified 69 AaHDZ transcription factors and systematically characterized their structural and physicochemical properties. The predicted amino acid sequence lengths range from 188 to 854 residues, with relative molecular masses of 22.2–94.6 kDa, theoretical isoelectric points of 4.65–9.31, and lipid solubility indices of 52.08–89.14. All of these proteins were hydrophilic. The results of subcellular localization predictions indicated that most AaHDZ proteins are localized in the nucleus, while a few are located in the cytoplasm and chloroplasts. Based on the classification of the HD-Zip family in <em>Arabidopsis thaliana</em>, the 69 AaHDZ proteins were divided into four subfamilies, with similar gene structures observed within each subfamily. Heterologous overexpression of <em>AaHDZ64</em> gene significantly increased trichome density in <em>A. thaliana</em>. The results confirmed that <em>AaHDZ64</em> is a positive regulatory factor for the growth and development of trichomes. This study provides a theoretical foundation for understanding the molecular regulatory mechanisms underlying trichome development in <em>A. argyi</em> leaves and offers insights for the precision breeding of <em>A. argyi</em>.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100529"},"PeriodicalIF":2.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing drought tolerance mechanisms in Pongamia pinnata through integrated physiological, biochemical, and transcriptomic profiling","authors":"K. Rajarajan ,&nbsp;Sandhya Sharma ,&nbsp;Harsha Srivastava ,&nbsp;Kumari Arpita ,&nbsp;A.K. Handa ,&nbsp;A. Arunachalam ,&nbsp;S.K. Dhyani","doi":"10.1016/j.plgene.2025.100527","DOIUrl":"10.1016/j.plgene.2025.100527","url":null,"abstract":"<div><div><em>Pongamia pinnata</em> is a promising industrial species for biofuel production. However, the detrimental effects of drought stress on the initial growth phases pose significant challenges to germination and seedling development. This problem impedes the establishment of commercial plantations in drought-prone areas, primarily because of the lack of cultivar stability. To address this issue, understanding their physiochemical and molecular responses is crucial. To elucidate the intricate molecular mechanisms underlying drought tolerance, two contrasting Pongamia genotypes, NRCP9 (tolerant) and NRCP10 (sensitive), were subjected to drought stress and watered conditions. Drought stress significantly reduced the chlorophyll content and relative water content in the NRCP10 (susceptible) genotype. In contrast, drought stress induced greater increases in peroxidase activity and proline accumulation in NRCP9 than in NRCP10. Furthermore, transcriptome analysis revealed a total of 26,195 and 18,742 differentially expressed genes (DEGs) in the tolerant and susceptible genotypes, respectively. Additionally, 128 common DEGs were commonly expressed under drought stress conditions, whereas 10,271 DEGs were commonly expressed under well-watered conditions. Among the DEGs in the TF families, the major were bHLH, NAC, ERF, WRKY, MYB, Trihelix, bZIP, FAR1, B3, C3H, STAT, and C2H2. Furthermore, transcriptome analyses revealed the significant genes involved in hormone biosynthesis, secondary metabolite accumulation, cofactor and carbon metabolism, and MAPK signaling. Additionally, the selected genes were validated by qRT-PCR, the transcriptome analysis and expression patterns were found to be corresponded. These findings reveal Pongamia's stress-adaptive mechanism and shed light on the physicochemical and differential gene responses to drought stress.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100527"},"PeriodicalIF":2.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular network, structural and expression analyses of NHX genes in Phaseolus vulgaris in response to salt stress","authors":"Faten Mhadhbi,&nbsp;Hatem Boubakri","doi":"10.1016/j.plgene.2025.100526","DOIUrl":"10.1016/j.plgene.2025.100526","url":null,"abstract":"<div><div>Salt stress is one of the most serious abiotic stresses limiting plant productivity worldwide. Here, we identified and characterized Sodium/Hydrogen antiporter (NHX) family in <em>Phaseolus vulgaris</em> genome and studied their expression in response to salt stress, for the first time, using RT q-PCR. In total, we identified 9 <em>PvNHX</em> genes from the common bean genome. Phylogeny, gene structure, motif patterns were performed and allowed the classification of these genes according to their subcellular localization into three subfamilies termed vacuolar (Vac-class), plasma membrane (PM-class) and endosomal (Endo-class). Conserved motifs and gene structures showed that most of which had an amiloride-binding site (FFI/LY/FLLPPI). RT q-PCR analysis revealed that salt stress induced the expression of specific <em>PvNHX</em> genes in both leaves (<em>PvNHX1</em>, <em>PvNHX2</em>, Pv<em>NHX3</em>, and <em>PvNHX7</em>) and roots (<em>PvNHX1</em>, <em>PvNHX2</em>, <em>PvNHX3</em>, <em>PvNHX4</em> and <em>PvNHX7</em>), suggesting their potential role in regulating salinity tolerance in <em>P. vulgaris.</em> Protein-protein interaction (PPI) network indicated that several PvNHX proteins interact with CIPK24 (CBL-interacting serine/threonine-protein kinase 24), CBL4 (Calcineurin B-like protein 4) and KEA4 (K⁺ efflux antiporter 4) proteins, implying their involvement in CBL-CIPK pathway during salinity adaptation. These findings provide valuable targets within the <em>PvNHX</em> family for genetic engineering aimed at improving salinity tolerance in <em>Phaseolus vulgaris</em>.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100526"},"PeriodicalIF":2.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 14-3-3 gene family in Nicotiana tabacum: Genome-wide identification, expression profiling, subcellular localization, and protein interaction analysis in response to cadmium stress","authors":"Tengfei Ma ,&nbsp;Kunjian Yang ,&nbsp;Fang Yang ,&nbsp;Li Chen ,&nbsp;Ji Chen ,&nbsp;Shunqin Zhu ,&nbsp;Wanhong Liu","doi":"10.1016/j.plgene.2025.100525","DOIUrl":"10.1016/j.plgene.2025.100525","url":null,"abstract":"<div><div>Plant 14-3-3 proteins play a crucial role in the fine-tuning of growth and development, stress resistance, and nutrient metabolism. Tobacco is recognized as a hyperaccumulator of cadmium (Cd), yet the understanding of the <em>Nt14-3-3</em> gene family remains limited in tobacco (<em>Nicotiana tabacum</em> L.). This study systematically identified 28 members (designated as NtGRF1 through NtGRF28) of the <em>Nt14-3-3</em> family in the cultivated variety TN90 and categorized them into ε and non-ε groups. Genes within the same evolutionary branch exhibited similar conserved motif patterns and intron-exon structures. Promoter <em>cis</em>-acting element analysis indicated that the expression of <em>NtGRF</em> genes is regulated by plant hormones, particularly abscisic acid (ABA) and jasmonic acid (JA), as well as various stress factors. RNA-seq-based analysis revealed three expression patterns for the <em>Nt14-3-3</em> family genes: constitutively high-expressing, low-expressing, and tissue-specific expression groups, corroborating these findings with qPCR results. Cd stress significantly enhanced the expression levels of six out of eight randomly screened <em>NtGRF</em> genes (<em>NtGRF8, NtGRF15, NtGRF16, NtGRF21, NtGRF22,</em> and <em>NtGRF28</em>). Subcellular localization analysis showed that <em>NtGRF8, NtGRF16,</em> and <em>NtGRF22</em> were expressed in the cytoplasm and nucleus, while <em>NtGRF26</em> was restricted to the cytoplasm. Yeast two-hybrid assays demonstrated that <em>NtGRF16</em> and <em>NtGRF22</em> formed homodimers, with heterodimer formation also prevalent. These results offer important insights into the potential biological functions of the <em>Nt14-3-3</em> gene family, emphasizing their role in various biological processes, particularly in the response of tobacco to cadmium stress.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100525"},"PeriodicalIF":2.2,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Azomite, a volcanic ash-based fertilizer modulates gene expression during photomorphogenesis through phyB-dependent and independent pathways","authors":"Elijah Mehlferber ,&nbsp;Kent F. McCue ,&nbsp;Yang Bi ,&nbsp;Robert Reed ,&nbsp;Jon Ferrel ,&nbsp;Rajnish Khanna","doi":"10.1016/j.plgene.2025.100523","DOIUrl":"10.1016/j.plgene.2025.100523","url":null,"abstract":"<div><div>Azomite is a lightly weathered dacitic (rhyolitic) tuff breccia (DTB), it is silicon-based with over 70 minerals and trace elements (micronutrients). In previous studies, application of Azomite increased greenhouse tomato production. In the tomato root endosome, Azomite caused functional shifts from higher abundance of microbes involved in metabolism of 2- to 4- carbon compounds to higher levels of microbes involved in carbohydrate metabolism. This suggested a possible increase in carbohydrate production and shift in exudates involved in microbial recruitment. Parallel studies with 4-day old Arabidopsis seedlings revealed that photosynthetically active radiation was required for Azomite-induced increase in both hypocotyl length and cotyledon area. These data suggested that Azomite may influence growth through changes in photosynthesis, leading to carbohydrate-enriched root exudates and increased growth. Here, we present RNAseq analysis in response to Azomite of 4-day old Arabidopsis seedlings grown either in continuous darkness (Dc) or under continuous red-light (Rc). Significant changes in genes involved in carbon assimilation and nutrient uptake, amongst other functional pathway categories are reported. Comparison with <em>phyB</em> (phytochrome B, red-light photoreceptor) mutant seedlings is shown to determine the overlap between phyB-regulated genes and Azomite-responsive genes. Two concentrations, 0.5 g and 1.0 g of Azomite were included because our previous results with tomato and Arabidopsis exhibited a dose-dependent response. Several genes are identified as responding differentially, including <em>SUBERMAN</em>, a myb-family transcription factor that regulates suberization of the root endodermis. This study advances our understanding of how complex mixtures of micronutrients such as Azomite influence gene expression during plant growth and development.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100523"},"PeriodicalIF":2.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reference gene selection and expression analyses of anthocyanin biosynthetic genes in flower and vegetative tissues of Clivia miniata L.","authors":"Mathabatha F. Maleka ,&nbsp;Johan J. Spies","doi":"10.1016/j.plgene.2025.100521","DOIUrl":"10.1016/j.plgene.2025.100521","url":null,"abstract":"<div><div>Pigmentation is one of the most variable traits in plants, with flowers typically being the main tissue that displays such diversity. Unlike other horticulturally valuable plants, the genetic basis of flower pigmentation in <em>Clivia</em> species remains poorly understood. Therefore, this study aimed to analyze the expression of numerous anthocyanin biosynthetic genes in flower and vegetative tissues of the most popular <em>Clivia</em> species, <em>Clivia miniata</em>. Such information can facilitate the breeding and biotechnological use of <em>Clivia</em> in the global horticulture value-chain. Initially, we mined a previously assembled <em>C. miniata</em> flower transcriptome for <em>WDR</em> transcripts that are homologous to key pigmentation genes from maize (<em>PAC1</em>) and <em>Arabidopsis thaliana</em> (<em>TTG1</em>). Subsequently, we identified and tested eight candidate reference genes (<em>18S</em>, <em>ACT</em>, <em>EF1</em>α, <em>G6PDH</em>, <em>PP2A</em>, <em>RNPII</em>, <em>TUB</em>α and <em>UBQ</em>) for expression stability in <em>Clivia</em> tissues using geNorm, NormFinder, BestKeeper, and the comparative Delta-Ct method. Homology analysis revealed a transcript encoding a partial protein with specific motifs that are common in pigmentation-related WDR proteins. Further, three reference genes (<em>CmiPP2A</em>, <em>CmiEF1</em>α and <em>Cmi18S</em>) were most stable in tested <em>Clivia</em> tissues. Quantitative real-time PCR (qPCR) analyses of ten anthocyanin biosynthetic genes (<em>CmiDFR</em>, <em>CmiF3H</em>, <em>CmiF3’H</em>, <em>CmiUF3GT</em>, <em>CmibHLH001</em>, <em>CmibHLH002</em>, <em>CmiMYB001</em>, <em>CmiMYB002</em>, <em>CmiMYB003</em> and <em>CmiWDR001</em>) normalized against the three reference genes revealed that most had relatively higher expression levels in flower than vegetative tissues. Also, expression was generally higher during anthesis than in buds, but some genes remarkably showed marked expression in roots too. Overall, this is the first study to systematically select and validate reference genes for expression analysis of anthocyanin biosynthetic genes in <em>Clivia</em>.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100521"},"PeriodicalIF":2.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144263937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of aldehyde dhydrogenase (ALDH) family genes in pepper (Capsicum annuum) reveals involvement of CaALDH7B1 in heat and drought stress tolerance","authors":"Bo Yang ,&nbsp;Yifen Shang ,&nbsp;Lang Wen ,&nbsp;Yijia Cui ,&nbsp;Zixing Li ,&nbsp;Yuan Cheng ,&nbsp;Chaochao Liu","doi":"10.1016/j.plgene.2025.100524","DOIUrl":"10.1016/j.plgene.2025.100524","url":null,"abstract":"<div><div>Abiotic stresses such as heat and drought lead to oxidative damage in plants by inducing excessive accumulation of reactive oxygen species (ROS). Aldehyde dehydrogenases (ALDHs), which detoxify reactive aldehydes, play critical roles in stress responses, but their functions in pepper (<em>Capsicum annuum</em>) remain largely unexplored. In this study, 28 CaALDH genes were identified and categorized into nine families. Phylogenetic and synteny analyses revealed strong evolutionary conservation, highlighting CaALDH2B4 and CaALDH7B1 as key members. Expression profiling showed distinct tissue-specific patterns and robust induction under heat and drought stress. Functional analysis via virus-induced gene silencing (VIGS) confirmed that CaALDH7B1 enhances stress tolerance by limiting ROS accumulation, promoting antioxidant enzyme activity (SOD, CAT, APX, POD), and maintaining NADPH/NADP⁺ homeostasis. Comparative genomics and structural modeling further revealed that CaALDH7B1 is evolutionarily conserved, with critical NADP⁺-binding residues retained across plant species. These findings underscore the pivotal role of CaALDH7B1 in oxidative stress regulation and provide new insights into the functional evolution of the ALDH gene family in pepper.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100524"},"PeriodicalIF":2.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The calmodulin-binding transcriptional activator transcription factor family in foxtail millet (Setaria italica L.): Molecular characterization, codon bias, and evolutionary trajectory","authors":"Huilong Chen ,&nbsp;Kexin Ji ,&nbsp;Yun Bai ,&nbsp;Yuxian Li ,&nbsp;Ying Liu ,&nbsp;Fang Liu ,&nbsp;Yutong Cui ,&nbsp;Weina Ge ,&nbsp;Zhenyi Wang","doi":"10.1016/j.plgene.2025.100522","DOIUrl":"10.1016/j.plgene.2025.100522","url":null,"abstract":"<div><div>Calmodulin-binding transcriptional activator (CAMTA) is a calmodulin-binding transcription factor, which plays an important role in calcium/calmodulin transduction signaling pathway. Foxtail millet (<em>Setaria italica</em> L.) is an economically important C4 model crop, but the genome-wide identification and molecular evolution of the CAMTA family is not yet available. In this study, seven CAMTA genes were identified from the foxtail millet genome via bioinformatics methods. They were overall similar in structure but differed, and all showed tissue expression specificity. Regarding molecular evolution, codon bias contributed to the evolution of the CAMTA family, and interestingly, base mutation was not the main factor affecting their codon bias, and may also be affected by factors such as natural selection and other factors. Multiple traces indicated that polyploidization events primarily led to the expansion of the plant CAMTA family, with overall similar but differentiated expansion histories in different species, and that <em>Selaginella moellendorfii</em> possesses the most paralogous genes being the most distinctive. More intriguingly, we found the opposite quantitative evolutionary history of terrestrial plants to that of the algal CAMTA family. Therefore, we firstly analyzed the molecular properties of the CAMTA transcription factor family in foxtail millet and explored its molecular evolutionary trajectory, and constructed a flow of evolutionary trajectories from monospecies transition to the plant kingdom for reference.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100522"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of medicinal plant Solanum procumbens Lour. using DNA barcodes and morphology","authors":"Lieu Thi Thuy Nguyen ,&nbsp;Quyen Thi Thao Tran ,&nbsp;Dan Thai Vo ,&nbsp;Tri Minh Bui ,&nbsp;Biet Van Huynh ,&nbsp;Dung Minh Ha-Tran","doi":"10.1016/j.plgene.2025.100517","DOIUrl":"10.1016/j.plgene.2025.100517","url":null,"abstract":"<div><h3>Background</h3><div>This study employed DNA barcoding to identify and differentiate <em>Solanum procumbens</em>, a potential medicinal plant used from other species in Solanaceae family. Eleven plant samples of <em>Solanum</em> spp. with morphological characteristics similar with those of <em>S. procumbens</em> and <em>S. trilobatum</em> were collected from various locations across Vietnam. Five DNA barcode markers were analyzed for identifying <em>Solanum procumbens</em> and its morphologically cryptic species <em>Solanum trilobatum</em>.</div></div><div><h3>Results</h3><div>Ten putative <em>S. procumbens</em> samples displayed a high identity (99.6–99.7 %) with the reference sequence within the transfer RNA leucine-transfer RNA phenylalanine <em>(trnL</em>–<em>trnF)</em> region. DNA sequences of the Internal Transcribed Spacer (ITS) region exhibited lower identity with the reference sequence, ranging from 94 % to 97.25 %. Despite this difference, DNA barcoding effectively differentiated these individuals, with both chloroplast <em>trnL</em>–<em>trnF</em> and nuclear ITS barcodes achieving high identification accuracy. The precision of <em>trnL</em>–<em>trnF</em> and ITS in species identification was validated using dried leaf samples of the putative <em>S. procumbens</em> and <em>S. trilobatum</em>. The consistency between fresh and dried samples confirms that our results are reliable and applicable in the pharmaceutical industry.</div></div><div><h3>Conclusions</h3><div>Phylogenetic analysis based on barcoding data aligned with morphology-based phenogram. These findings demonstrate the effectiveness of DNA barcoding for accurate identification of <em>S. procumbens</em>, particularly when morphological traits are ambiguous.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100517"},"PeriodicalIF":2.2,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}