Genome-wide identification of HD-Zip transcription factor family in Artemisia argyi and functional analysis of AaHDZ64 in trichome development

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2025-06-27 DOI:10.1016/j.plgene.2025.100529

Zhanhu Cui , Chunyan Miao , Heyang Shang , Qian Zhao , Shujiao Li , Zhongyi Zhang , Yuqing Wang , Xianzhang Huang

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Abstract

The homeodomain-leucine zipper (HD-Zip) family of transcription factor is a unique and vital gene family in plants, involved in various processes including organ development, stress responses, and regulation of secondary metabolite biosynthesis. This study aimed to investigate the role of HD-Zip family transcription factors in the development of Artemisia argyi trichomes. By utilizing the whole genome data of A. argyi, we identified 69 AaHDZ transcription factors and systematically characterized their structural and physicochemical properties. The predicted amino acid sequence lengths range from 188 to 854 residues, with relative molecular masses of 22.2–94.6 kDa, theoretical isoelectric points of 4.65–9.31, and lipid solubility indices of 52.08–89.14. All of these proteins were hydrophilic. The results of subcellular localization predictions indicated that most AaHDZ proteins are localized in the nucleus, while a few are located in the cytoplasm and chloroplasts. Based on the classification of the HD-Zip family in Arabidopsis thaliana, the 69 AaHDZ proteins were divided into four subfamilies, with similar gene structures observed within each subfamily. Heterologous overexpression of AaHDZ64 gene significantly increased trichome density in A. thaliana. The results confirmed that AaHDZ64 is a positive regulatory factor for the growth and development of trichomes. This study provides a theoretical foundation for understanding the molecular regulatory mechanisms underlying trichome development in A. argyi leaves and offers insights for the precision breeding of A. argyi.

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艾蒿HD-Zip转录因子家族的全基因组鉴定及AaHDZ64在毛状体发育中的功能分析

同源域-亮氨酸拉链（HD-Zip）转录因子家族是植物中一个独特而重要的基因家族，参与了器官发育、逆境反应和次生代谢产物生物合成的调控等多种过程。本研究旨在探讨HD-Zip家族转录因子在艾叶毛状体发育中的作用。利用艾叶的全基因组数据，鉴定出69个AaHDZ转录因子，并对其结构和理化性质进行了系统表征。预测氨基酸序列长度为188 ~ 854个残基，相对分子质量为22.2 ~ 94.6 kDa，理论等电点为4.65 ~ 9.31，脂溶性指数为52.08 ~ 89.14。所有这些蛋白质都是亲水的。亚细胞定位预测结果表明，大多数AaHDZ蛋白定位于细胞核，少数位于细胞质和叶绿体中。根据拟南芥HD-Zip家族的分类，将69个AaHDZ蛋白划分为4个亚家族，每个亚家族中都有相似的基因结构。AaHDZ64基因的异源过表达显著增加了拟蓝藻的毛密度。结果证实，AaHDZ64是毛状体生长发育的正向调控因子。本研究为了解艾叶毛状体发育的分子调控机制提供了理论基础，并为艾叶精细育种提供了参考。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.