药用植物天竺葵的鉴定。利用DNA条形码和形态学

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2025-05-26 DOI:10.1016/j.plgene.2025.100517

Lieu Thi Thuy Nguyen , Quyen Thi Thao Tran , Dan Thai Vo , Tri Minh Bui , Biet Van Huynh , Dung Minh Ha-Tran

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引用次数: 0

摘要

摘要本研究利用DNA条形码技术对茄科植物中具有潜在药用价值的原蛮茄（Solanum procumbens）进行鉴定和区分。在越南不同地点采集了11种形态特征与原藜和三叶草相似的茄属植物标本。对5种DNA条形码标记进行了分析，用于鉴别原甘菊及其形态隐种三叶甘菊。结果10份推定样品与参考序列在转移RNA亮氨酸-转移RNA苯丙氨酸（trnL-trnF）区具有较高的一致性（99.6 ~ 99.7%）。ITS区域的DNA序列与参考序列的一致性较低，在94% ~ 97.25%之间。尽管存在这种差异，但DNA条形码可以有效地区分这些个体，叶绿体trnL-trnF和核ITS条形码都具有很高的识别精度。利用推定的原藜和三叶草的干叶样品，验证了trnL-trnF和ITS在物种鉴定中的精度。新鲜和干燥样品之间的一致性证实了我们的结果是可靠的，适用于制药行业。结论基于条形码数据的系统发育分析与基于形态学的表型图一致。这些发现证明了DNA条形码在准确鉴定原藜的有效性，特别是在形态特征不明确的情况下。

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Identification of medicinal plant Solanum procumbens Lour. using DNA barcodes and morphology

Background

This study employed DNA barcoding to identify and differentiate Solanum procumbens, a potential medicinal plant used from other species in Solanaceae family. Eleven plant samples of Solanum spp. with morphological characteristics similar with those of S. procumbens and S. trilobatum were collected from various locations across Vietnam. Five DNA barcode markers were analyzed for identifying Solanum procumbens and its morphologically cryptic species Solanum trilobatum.

Results

Ten putative S. procumbens samples displayed a high identity (99.6–99.7 %) with the reference sequence within the transfer RNA leucine-transfer RNA phenylalanine (trnL–trnF) region. DNA sequences of the Internal Transcribed Spacer (ITS) region exhibited lower identity with the reference sequence, ranging from 94 % to 97.25 %. Despite this difference, DNA barcoding effectively differentiated these individuals, with both chloroplast trnL–trnF and nuclear ITS barcodes achieving high identification accuracy. The precision of trnL–trnF and ITS in species identification was validated using dried leaf samples of the putative S. procumbens and S. trilobatum. The consistency between fresh and dried samples confirms that our results are reliable and applicable in the pharmaceutical industry.

Conclusions

Phylogenetic analysis based on barcoding data aligned with morphology-based phenogram. These findings demonstrate the effectiveness of DNA barcoding for accurate identification of S. procumbens, particularly when morphological traits are ambiguous.

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.