Molecular network, structural and expression analyses of NHX genes in Phaseolus vulgaris in response to salt stress

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2025-06-18 DOI:10.1016/j.plgene.2025.100526

Faten Mhadhbi, Hatem Boubakri

{"title":"Molecular network, structural and expression analyses of NHX genes in Phaseolus vulgaris in response to salt stress","authors":"Faten Mhadhbi, Hatem Boubakri","doi":"10.1016/j.plgene.2025.100526","DOIUrl":null,"url":null,"abstract":"<div><div>Salt stress is one of the most serious abiotic stresses limiting plant productivity worldwide. Here, we identified and characterized Sodium/Hydrogen antiporter (NHX) family in Phaseolus vulgaris genome and studied their expression in response to salt stress, for the first time, using RT q-PCR. In total, we identified 9 PvNHX genes from the common bean genome. Phylogeny, gene structure, motif patterns were performed and allowed the classification of these genes according to their subcellular localization into three subfamilies termed vacuolar (Vac-class), plasma membrane (PM-class) and endosomal (Endo-class). Conserved motifs and gene structures showed that most of which had an amiloride-binding site (FFI/LY/FLLPPI). RT q-PCR analysis revealed that salt stress induced the expression of specific PvNHX genes in both leaves (PvNHX1, PvNHX2, PvNHX3, and PvNHX7) and roots (PvNHX1, PvNHX2, PvNHX3, PvNHX4 and PvNHX7), suggesting their potential role in regulating salinity tolerance in P. vulgaris. Protein-protein interaction (PPI) network indicated that several PvNHX proteins interact with CIPK24 (CBL-interacting serine/threonine-protein kinase 24), CBL4 (Calcineurin B-like protein 4) and KEA4 (K+ efflux antiporter 4) proteins, implying their involvement in CBL-CIPK pathway during salinity adaptation. These findings provide valuable targets within the PvNHX family for genetic engineering aimed at improving salinity tolerance in Phaseolus vulgaris.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100526"},"PeriodicalIF":2.2000,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Gene","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S235240732500037X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}

引用次数: 0

Abstract

Salt stress is one of the most serious abiotic stresses limiting plant productivity worldwide. Here, we identified and characterized Sodium/Hydrogen antiporter (NHX) family in Phaseolus vulgaris genome and studied their expression in response to salt stress, for the first time, using RT q-PCR. In total, we identified 9 PvNHX genes from the common bean genome. Phylogeny, gene structure, motif patterns were performed and allowed the classification of these genes according to their subcellular localization into three subfamilies termed vacuolar (Vac-class), plasma membrane (PM-class) and endosomal (Endo-class). Conserved motifs and gene structures showed that most of which had an amiloride-binding site (FFI/LY/FLLPPI). RT q-PCR analysis revealed that salt stress induced the expression of specific PvNHX genes in both leaves (PvNHX1, PvNHX2, PvNHX3, and PvNHX7) and roots (PvNHX1, PvNHX2, PvNHX3, PvNHX4 and PvNHX7), suggesting their potential role in regulating salinity tolerance in P. vulgaris. Protein-protein interaction (PPI) network indicated that several PvNHX proteins interact with CIPK24 (CBL-interacting serine/threonine-protein kinase 24), CBL4 (Calcineurin B-like protein 4) and KEA4 (K⁺ efflux antiporter 4) proteins, implying their involvement in CBL-CIPK pathway during salinity adaptation. These findings provide valuable targets within the PvNHX family for genetic engineering aimed at improving salinity tolerance in Phaseolus vulgaris.

查看原文本刊更多论文

盐胁迫下菜豆NHX基因的分子网络、结构及表达分析

盐胁迫是世界范围内限制植物生产力的最严重的非生物胁迫之一。本研究首次利用RT - q-PCR技术，鉴定了菜豆（Phaseolus vulgaris）基因组中钠/氢反转运蛋白（Sodium/Hydrogen antiporter， NHX）家族，并对其在盐胁迫下的表达进行了研究。我们一共从普通豆基因组中鉴定出9个PvNHX基因。系统发育、基因结构、基序模式进行了分析，并根据亚细胞定位将这些基因分为三个亚家族，即空泡亚家族（vac类）、质膜亚家族（pm类）和内体亚家族（endo类）。保守的基序和基因结构表明，其中大部分具有一个酰胺结合位点（FFI/LY/FLLPPI）。RT - q-PCR分析结果显示，盐胁迫诱导PvNHX基因在植物叶片（PvNHX1、PvNHX2、PvNHX3和PvNHX7）和根系（PvNHX1、PvNHX2、PvNHX3、PvNHX4和PvNHX7）中表达，提示PvNHX基因可能在盐胁迫下调控植物耐盐性。蛋白质-蛋白质相互作用（PPI）网络表明，PvNHX蛋白与CIPK24 （cbl -相互作用丝氨酸/苏氨酸-蛋白激酶24）、CBL4（钙调磷酸酶b样蛋白4）和KEA4 （K+外排反转运蛋白4）蛋白相互作用，暗示它们在盐度适应过程中参与了CBL-CIPK途径。这些发现为提高菜豆耐盐性的基因工程提供了PvNHX家族有价值的靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.