Genome-wide characterization of the anthocyanin O-methyltransferases in the cultivated potato

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2025-04-03 DOI:10.1016/j.plgene.2025.100506

Annalisa Staiti , Carmine Fruggiero , Domenico Carputo , Vincenzo D’Amelia , Nunzio D’Agostino

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引用次数: 0

Abstract

O-methyltransferases (OMTs) are group of enzymes involved in the methylation of various secondary metabolites, including anthocyanins. This secondary modification, together with hydroxylation and glycosylation, affects the chromatic properties, stability and reactivity of these pigments. Meanwhile, no detailed identification or genome-wide analysis of the OMT gene family members in potato (Solanum tuberosum) has been reported. We conducted a genome-wide identification and characterization of potato OMTs, identifying 65 OMT family members. These were characterized based on gene structure, evolutionary relationships, and promoter motifs. Analysis of available gene expression profiles from public databases revealed expression patterns indicative of tissue- and temporal-specificity Finally, we further elucidated expression differences among four selected OMT-encoding genes using potato cell cultures, which represent a smart and gainful alternative for anthocyanin production and deep knowledge about these post-biosynthetic modifications.

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栽培马铃薯花青素o -甲基转移酶的全基因组特征

o -甲基转移酶（OMTs）是一组参与各种次生代谢产物甲基化的酶，包括花青素。这种二级修饰，连同羟基化和糖基化，影响这些颜料的着色性能、稳定性和反应性。同时，马铃薯（Solanum tuberosum） OMT基因家族成员的详细鉴定和全基因组分析尚未见报道。我们对马铃薯OMT进行了全基因组鉴定和表征，鉴定了65个OMT家族成员。这些是基于基因结构、进化关系和启动子基序来鉴定的。通过对公共数据库中可用基因表达谱的分析，揭示了组织特异性和时间特异性的表达模式。最后，我们利用马铃薯细胞培养进一步阐明了四个选定的omt编码基因之间的表达差异，这代表了花青素生产的一个聪明而有益的选择，并深入了解了这些生物合成后修饰。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.