Genome-wide analysis of receptor-like 2 kinase 1-like genes reveals involvement of a RLK1L member in tolerance to salt stress in Medicago sativa

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2025-05-23 DOI:10.1016/j.plgene.2025.100520

Yiping Wei , Tian Zhang , Qinyan Bao, Zhaozhu Wen, Shuzhi Ma, Yanbo Zhang, Wenxuan Du

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引用次数: 0

Abstract

The Catharanthus roseus receptor-like kinase 1-like (CrRLK1L) family constitutes a specialized group within plant receptor-like protein kinases that has been detected in numerous plant varieties. However, RLK1L members have not been characterized in alfalfa. Through systematic analysis, this study examined the features and identification of RLK1L genes within alfalfa based on known gene sequences from the model plants within the Arabidopsis genus, specifically Arabidopsis thaliana, and those in the Medicago genus, such as Medicago truncatula. Phylogenetic analysis of AtCrRLK1Ls, MtCrRLK1Ls, and MsCrRLK1Ls led to the classification of 19 MsCrRLK1L genes in alfalfa into eight distinct clusters. Bioinformatic analyses revealed that the molecular masses of MsCrRLK1L proteins spanned from 29.17 to 167.36 kDa. Subcellular targeting predictions indicated predominant cell membrane localization for these polypeptides Conserved domain profiling demonstrated the ubiquitous presence of a malectin-like domain across all MsCrRLK1L members. Chromosome localization and collinearity analysis showed that the 19 MsCrRLK1L genes were unevenly distributed across five alfalfa chromosomes, with four gene pairs exhibiting intra-species collinearity. Cis-Acting element analysis identified stress- and hormone-responsive motifs, suggesting that MsCrRLK1Ls are involved in abiotic stress signaling. Gene expression profiling under salt stress (200 mM NaCl) demonstrated tissue-specific induction patterns, with six MsCrRLK1L genes significantly upregulated in aerial and root tissues. Furthermore, MsCrRLK1L2 overexpression in Arabidopsis improved the germination rate and growth status under salt stress. This study comprehensively profiles the MsCrRLK1L family in alfalfa, confirming its role in salt tolerance and suggesting genetic targets to boost crop resilience.

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受体样2激酶1样基因的全基因组分析揭示了RLK1L成员参与苜蓿对盐胁迫的耐受性

Catharanthus roseus receptor-like kinase 1-like （CrRLK1L）家族是植物受体样蛋白激酶中的一个特殊类群，已在许多植物品种中检测到。然而，RLK1L成员尚未在苜蓿中被表征。本研究通过系统分析，利用拟南芥属模式植物（特别是拟南芥）和紫花苜蓿属模式植物（如truncatula紫花苜蓿）的已知基因序列，对紫花苜蓿RLK1L基因的特征和鉴定进行了研究。通过对AtCrRLK1Ls、MtCrRLK1Ls和MsCrRLK1Ls的系统发育分析，将苜蓿中19个MsCrRLK1L基因分为8个不同的基因簇。生物信息学分析显示，MsCrRLK1L蛋白的分子质量在29.17 ~ 167.36 kDa之间。亚细胞靶向预测表明这些多肽的主要细胞膜定位保守结构域分析表明，在所有MsCrRLK1L成员中普遍存在雄性凝集素样结构域。染色体定位和共线性分析表明，19个MsCrRLK1L基因不均匀分布在5条苜蓿染色体上，其中4对基因在种内共线性。顺式作用元件分析确定了应激和激素反应基序，表明mscrrlk1l参与非生物应激信号传导。盐胁迫（200 mM NaCl）下的基因表达谱显示出组织特异性诱导模式，6个MsCrRLK1L基因在地上和根组织中显著上调。此外，MsCrRLK1L2在拟南芥中的过表达改善了盐胁迫下的发芽率和生长状况。本研究全面分析了苜蓿中MsCrRLK1L家族，证实了其在耐盐性中的作用，并提出了提高作物抗逆性的遗传靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.