Plant Gene最新文献

{"title":"Genome-wide identification and characterisation of hevein precursor genes in Hevea brasiliensis","authors":"Li Ying Chang,&nbsp;Hui Wen Lee,&nbsp;Gincy P. Thottathil,&nbsp;Kumar Sudesh","doi":"10.1016/j.plgene.2024.100455","DOIUrl":"https://doi.org/10.1016/j.plgene.2024.100455","url":null,"abstract":"<div><p><em>Hevea brasiliensis</em> is the primary source of natural rubber, which is obtained from its latex and used in the manufacturing of various products. Hevein is a small protein found in the latex, produced by post-translational cleavage of prohevin, which is encoded by the hevein precursor gene. It exhibits antimicrobial and agglutination properties. Several nucleotide sequences that encode the hevein precursor genes were reported, however, the sequences are not characterised well. In the present study, four full-length hevein precursor sequences (<em>Hevein 1</em>–<em>4</em>) were obtained by manually curating the sequences from different databases. All sequences show high homology with the highest identity between <em>Hevein 1</em> and <em>Hevein 3</em>. In a phylogenetic analysis along with sequences from related plants, all sequences from <em>H. brasiliensis</em> were clustered into a specific clade. All hevein precursor genes were expressed in the latex samples obtained from three clones; RRIM 600, RRIM 3001 and PB 350 of less than one year old and five-year-old RRIM 3001 plants, grown in plant house, as well as field-grown trees of clone PB 350 belonging to three different ages; two years, six years and fifteen years. All hevein precursor genes in the five-year-old RRIM 3001 plants with stunted growth, maintained in the plant house showed alternative splicing. <em>Hevein 3</em> was expressed with two splice variants, one with intron retention and the other without intron whereas the other genes were expressed with only intron retained variant. Differential expression analysis using nanoplate digital PCR showed that <em>Hevein 2</em> and <em>Hevein 3</em> were expressed with no significant difference among the three young <em>H. brasiliensis</em> clones. The expression of <em>Hevein 2</em> and <em>Hevein 3</em> among the <em>H. brasiliensis</em> clone PB 350 of different ages grown in field conditions showed significant difference. The present study provides a better understanding on the importance of hevein precursor genes in different physiological responses which will be useful for further research leading to the genetic improvement of <em>H. brasiliensis</em>.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"38 ","pages":"Article 100455"},"PeriodicalIF":0.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140550788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tapetum-specific expression of cysteine protease induces male sterility in tomato","authors":"Phanikanth Jogam ,&nbsp;Pandarinath Savitikadi ,&nbsp;Dulam Sandhya ,&nbsp;Raghu Ellendula ,&nbsp;Venkataiah Peddaboina ,&nbsp;Venkateswar Rao Allini ,&nbsp;Sadanandam Abbagani","doi":"10.1016/j.plgene.2024.100454","DOIUrl":"https://doi.org/10.1016/j.plgene.2024.100454","url":null,"abstract":"<div><p>Male sterile plants play a significant role in developing hybrid varieties to exploit the benefits of hybrid vigour in crops. Cysteine proteases play critical functions, including proteolysis and programmed cell death in plants. In this study, we have generated male-sterile transgenic tomato plants using <em>AdCP</em> (<em>Arachis diogoi cysteine protease</em>) gene under the control of a tapetum-specific promoter (TA-29). The transgenic tomato plants produced non-functional pollen grains. The aborted pollen grains of the male sterile plant did not germinate even after 24 h of incubation compared to normal pollen grains. PCR analysis confirmed the stable integration of transgenes in transgenic plants. Semi-quantitave RT-PCR analysis showed the tissue-specific <em>AdCP</em> gene expression in the anthers of transgenic tomato plants. A back-cross was conducted between the transgenic male-sterile plants (female parent) and control (untransformed) plants (male parent). The T1 progeny indicated the segregation into female fertile and male-sterile plants, showing normal fruit development and seed set. High levels of <em>AdCP</em> transcripts were detected in anther tissues, confirming tapetum-specific expression of the TA29 promoter. The male-sterile tomato plants with targeted expression of the AdCP gene in tapetum could potentially be used to develop novel varieties through hybrid seed production.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"38 ","pages":"Article 100454"},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140537102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silicon transporters in plants: Unravelling the molecular Nexus with sodium and potassium transporters under salinity stress","authors":"Heba T. Ebeed ,&nbsp;Hanan S. Ahmed ,&nbsp;Nemat M. Hassan","doi":"10.1016/j.plgene.2024.100453","DOIUrl":"https://doi.org/10.1016/j.plgene.2024.100453","url":null,"abstract":"<div><p>Salt stress poses a serious hazard to plant growth by altering osmotic and ionic homeostasis, producing too many oxidants and radicals, and harming vital metabolic processes like photosynthesis. Plants use mechanistic cascades of biochemical and physiological processes to battle salt stress and prevent ion toxicity; nevertheless, repeated exposure can overwhelm the defence system, leading to plant death. The Salt-Overly Sensitive (SOS) pathway, which predominantly relies on Na⁺ exclusion from the cytosol, makes a significant contribution to salinity tolerance in plants. Although silicon (Si) is known to reduce salt stress in a variety of crops and to raise plant stress tolerance, its impact on Na⁺ transport is little understood. In this review, we emphasise recent research on the interaction between Si treatment and important Na⁺ and K⁺ transporters involved in ion homeostasis under salt stress. The following aspects will receive special consideration: (1) The effects of salinity on membrane stability and ion homeostasis and the involvement of Na⁺ and K⁺ transporters in ion homeostasis (2) The uptake, storage, and transport of Si in higher plants, as well as the discovered Si transporters in many plant species (3) Modulation of the expression of the Na⁺, K⁺, and Si transporters to affect the absorption, transport, and homeostasis of ions by Si. Finally, this review also highlights the necessity for further investigation into the function of Si in salt stress in plants and the discovery of knowledge gaps in the broader area of this process.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"38 ","pages":"Article 100453"},"PeriodicalIF":0.0,"publicationDate":"2024-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140295870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the features and functions of serine/arginine protein kinases in bread wheat","authors":"Deepika,&nbsp;Madhu,&nbsp;Santosh Kumar Upadhyay","doi":"10.1016/j.plgene.2024.100451","DOIUrl":"10.1016/j.plgene.2024.100451","url":null,"abstract":"<div><p>Serine/arginine protein kinases (SRPKs) are members of the serine-threonine kinase family that phosphorylate the Serine/arginine-rich (SR) proteins involved in alternate splicing. They are reported in various eukaryotes including mammals, and in a few plants, but seldom explored in important crop species. Herein, we identified a total of nine <em>TaSRPK</em> genes from all three subgenomes (A, B, and D) of a staple crop <em>Triticum aestivum,</em> and phylogenetically classified them into two groups. The <em>TaSRPKs</em> have conserved gene architecture with four exons. Each TaSRPK protein consists of a characteristic protein kinase domain having an active site and ATP binding region. The occurrence of diverse <em>cis-</em>regulatory elements in the promoter region, and interaction with assorted groups of transcription factors and miRNAs exhibited their divergent functions. Differential expression of certain <em>TaSRPKs</em> in vegetative and reproductive tissues and in the presence of fungal pathogens and various abiotic stress conditions further assured their association during development processes and stress response. Our study highlighted the importance of TaSRPKs, which might be useful for their detailed characterization in future research.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"38 ","pages":"Article 100451"},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139829205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possible role of plant nucleoredoxins in context of ‘stress memory adaptation’ for oxidative stress as revealed by in-silico and citation network analysis","authors":"Arvind Gupta ,&nbsp;Rahul Thakur ,&nbsp;Saurabh Yadav","doi":"10.1016/j.plgene.2024.100452","DOIUrl":"10.1016/j.plgene.2024.100452","url":null,"abstract":"<div><p>Plants being sessile constantly phase encountered stresses throughout their life both abiotic and biotic stresses. Due to these stresses, plants need to possess some memory and currently there is need to correlate ecological constraints and importance of stress memory. Stress priming deals with the plant's capacity to memorize stress onslaught and adapt to recurring stress. Oxidative stress is one such abiotic stress which disturbs the cell homeostasis and redox balance inside the cell which is thereby countered by plant's antioxidant machinery. Thus, plants need to have some kind of memory to encounter future oxidative stress. Redoxins such as glutaredoxin (GRX), thioredoxin (TRX), peroxiredoxin (PRX), nucleoredoxin (NRX) <em>etc.</em> are enzymatic antioxidants which plays vital role in the plant growth and development. NRXs have been lesser characterized in plants as per existing literature and scientometric analysis in present work sheds light on the importance of plant NRXs. Nucleoredoxin, possessing antioxidant properties and the ability to scavenge ROS, may play a crucial role in molecular priming mechanisms. The citation network analysis using VOSviewer server also showed the importance of current research and relation of NRXs with terms like ROS, gene expression regulation, plant gene, phytohormones and plant immunity. Later the <em>Cicer arietinum</em> NRX sequence was bioinformatically characterized using several tools for better understanding. Currently, there is growing evidence and research on this ‘stress memory concept’ and how different molecular players are related to it.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"38 ","pages":"Article 100452"},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139883054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of high temperature stress on metabolome and aroma in rice grains","authors":"Anurag Mishra ,&nbsp;Braj Bhushan Singh ,&nbsp;Najam Akhtar Shakil ,&nbsp;M.D. Shamim ,&nbsp;Fozia Homa ,&nbsp;Rajat Chaudhary ,&nbsp;Prashant Yadav ,&nbsp;Deepti Srivastava ,&nbsp;Parveen Fatima ,&nbsp;Vandana Sharma ,&nbsp;Manoj Kumar Yadav ,&nbsp;Pushpendra Kumar","doi":"10.1016/j.plgene.2024.100450","DOIUrl":"10.1016/j.plgene.2024.100450","url":null,"abstract":"<div><p>Heat stress poses a significant challenge to global rice production, affecting yield and grain quality. Elevated temperatures during the flowering and grain-filling stages, both day and night, lead to reduced yield and compromised grain quality. This impact is more pronounced during nighttime high-temperature stress, seriously threatening rice productivity. With global temperatures rising, there is a looming threat to rice production. Aromatic rice, prized for superior aroma and grain quality, is particularly vulnerable to heat. Therefore, the present work has been carried out to investigate how high temperature affects the aromatic metabolites in rice grains among the 15 rice genotypes (fourteen aromatic and one non-aromatic rice i.e., Nagina 22). Results from the present study indicated that the inactive (mutated) <em>BADH2</em> gene expression was down-regulated under high-temperature stress conditions and no 2-acetyl-1-pyrroline (2-AP) accumulation was detected in the selected rice genotypes. However, the increase in levels of L-proline (precursor molecule for 2-AP) was detected, and due to the down-regulation of inactive <em>BADH2</em>, the oxidation of L-proline into 2-AP was affected. Proline amino acid significantly increased under high temperatures, impacting aroma quality. Metabolome studies revealed variations in compound detection among scented rice genotypes. Understanding these metabolites aids in addressing the loss of aroma in fragrant rice genotypes, offering insights into developing stable aromatic rice varieties under elevated temperature conditions. The study aims to identify metabolites causing aroma loss in aromatic rice. Results will aid in understanding aroma depletion mechanisms in scented rice under high-temperature stress, guiding the development of a stable aromatic rice variety in elevated temperatures.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"38 ","pages":"Article 100450"},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139872005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the dynamics of starch metabolism and expression profiles of starch synthesis genes in millet under drought stress","authors":"Joseph N. Amoah ,&nbsp;Monica Ode Adu-Gyamfi ,&nbsp;Albert Owusu Kwarteng","doi":"10.1016/j.plgene.2024.100449","DOIUrl":"https://doi.org/10.1016/j.plgene.2024.100449","url":null,"abstract":"<div><p>Drought impacts global food production, prompting extensive research to understand drought tolerance in millet. However, knowledge regarding the extent of tolerance achievable through acclimation remains limited. The objective of the study is to assess the effect of drought acclimation (hardening) on drought tolerance in millet and to investigate the physiological, biochemical, and transcriptional changes associated with starch metabolism in millet. To achieve this aim, two millet genotypes (‘PI 689680’ and ‘PI 662292’), exhibiting differential responses to drought stress, were subjected to various treatments: control (unstressed), drought acclimation (DA; two stress episodes with recovery), and non-acclimation (NA; a single stress episode with no recovery).. The study revealed that drought-induced oxidative stress, manifested by increased amylose, amylopectin, and total starch accumulation in NA plants compared to DA counterparts. Additionally, NA plants experienced a notable reduction in growth and photosynthetic activity. Expression patterns of starch-related transcripts were relatively elevated in NA compared to DA plants. These findings highlighted that acclimation to drought conferred tolerance to subsequent stress events by mitigating oxidative damage induced by drought stress. DA plants exhibited improved tolerance, characterized by enhanced growth, net photosynthetic rate, stomatal activity, osmotic adjustment, starch accumulation, enzyme activity, and the regulated expression of related genes. The study advocates for adopting acclimation as a strategic approach to mitigate the adverse effects of metabolic disruptions induced by drought in millet.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"38 ","pages":"Article 100449"},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352407324000040/pdfft?md5=0d43f7be446c530ae3ca3969779d8ed1&pid=1-s2.0-S2352407324000040-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139726567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative and integrative omic analysis focused on chaperones and interactors in a cultivated and an exotic tomato at different fruit ripening stages","authors":"Valentina Goytia Bertero ,&nbsp;Paolo Cacchiarelli ,&nbsp;Guillermo Raúl Pratta ,&nbsp;Débora Pamela Arce","doi":"10.1016/j.plgene.2024.100448","DOIUrl":"https://doi.org/10.1016/j.plgene.2024.100448","url":null,"abstract":"<div><p><span><span><span>Heat Shock Proteins (HSPs) are a superfamily of chaperones that have been characterized in different organisms. In plants, HSPs promote protein folding and deaggregation during </span>abiotic stress or developmental changes. The aim of this work was to integrate several omic-data to identify chaperone and putative </span>interactors in </span><span><em>Solanum</em><em> lycopersicum</em></span> domesticated cultivar Caimanta (C) and in the latinoamerican wild <em>Solanum pimpinellifolium</em><span><span><span> (P) genotypes during fruit ripening<span><span> (FR), which are the parental lines of different breeding populations obtained by our research group. We were able to identify newly putative interactors and simultaneously induced HSP members at the transcription and proteomic levels. This integrative approach also revealed gene/protein families related to chlorophyll content, </span>photosynthesis and HSP70 chaperones in C. Furthermore, P was enriched with chaperones, including HSP20, </span></span>ATPase<span> families, (characteristic of HSP90 and HSP100) and other protein families involved in oxidoreductase activity, supporting the hypothesis of the existence of a relationship between HSPs and developmental processes as FR. Finally, we found that some of these up-regulated chaperones show the presence of heat shock element motifs in their promoters. Proteomic coupled with </span></span>transcriptomics and interactomics facilitated the exploration of a good new gene-context at the tomato development.</span></p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"37 ","pages":"Article 100448"},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139674312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The complete chloroplast genome sequence of Lithospermum erythrorhizon: Insights into the phylogenetic relationship among Boraginaceae species and the maternal lineages of purple gromwells","authors":"Takahiro Okada ,&nbsp;Keiichi Watanabe","doi":"10.1016/j.plgene.2024.100447","DOIUrl":"https://doi.org/10.1016/j.plgene.2024.100447","url":null,"abstract":"<div><p>In Japan, <span><em>Lithospermum erythrorhizon</em></span><span><span><span> grows in the wild, and its roots are traditionally used for dyeing and medicinal purposes. However, due to excessive harvesting and changes in the natural environment, the population of this species has significantly declined over the past decades. To conserve the domestic varieties, it is important to obtain genomic information that accurately represents their pure lineage. The objective of this study was to characterize the chloroplast genome, which serves as a valuable </span>phylogenetic<span> marker, using next-generation sequencing. The results revealed that the DNA<span> has a typical quadripartite structure, spanning 150,478 bp with a GC content of 35.5%. A total of 113 unique genes are encoded, including 80 protein-coding genes, 4 </span></span></span>ribosomal RNA genes, and 29 transfer RNA genes. Comparative plastome analyses involving 13 </span><span><em>Boraginaceae</em></span> species, including <em>L. erythrorhizon</em><span>, showed high similarities in the gene order and codon usage, while an accelerated substitution rate was observed in </span><em>matK</em>. Phylogenetic analyses using this gene and 71 common protein-coding genes indicated a close evolutional relationship between <em>L. erythrorhizon</em> and <em>Glandora prostrata</em><span>. Furthermore, when comparing the chloroplast genome assembly data of a Chinese variety, a total of 44 structural variants were identified. Most of these variants were mononucleotide or dinucleotide in size, but a 70 bp insertion/deletion was identified in the intergenic region flanked by the </span><em>accD</em> and <em>psaI</em><span> genes. The presence of this relatively substantial structural variant indicates that the maternal lineages of the Japanese and Chinese varieties examined in this study are distinctly different.</span></p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"37 ","pages":"Article 100447"},"PeriodicalIF":0.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139674886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of black rice OsC1 confers tissue-specific anthocyanin accumulation in indica rice cv. Kasalath and its potential use as a visible marker in rice transformation","authors":"Chotipa Sakulsingharoj ,&nbsp;Supachai Vuttipongchaikij ,&nbsp;Kanogporn Khammona ,&nbsp;Lalita Narachasima ,&nbsp;Roypim Sukkasem ,&nbsp;Saengtong Pongjaroenkit ,&nbsp;Varaporn Sangtong ,&nbsp;Srimek Chowpongpang","doi":"10.1016/j.plgene.2024.100446","DOIUrl":"10.1016/j.plgene.2024.100446","url":null,"abstract":"<div><p><span><span>Anthocyanin </span>biosynthesis<span>, a process regulated by distinct MYB and MYC transcription factors, plays a crucial role in determining pigmentation in various tissue of plants. This study aimed to investigate the impact of overexpressing the </span></span><em>OsC1</em><span><span> gene from black rice, encoding a MYB transcription factor, on anthocyanin pigmentation in red indica rice cv. Kasalath. Anthocyanin pigmentation was readily observed as purple spots in calli and as purple shoot tips and purple leaf sheath in </span>transgenic seedlings. We confirmed the presence of the transgene using GUS assay and PCR analysis, and the pigmentation segregated following a 3:1 Mendelian ratio. T</span>₀ and T₁<span> transgenic plants<span> exhibits anthocyanin accumulation in various tissues including leaf sheaths, auricles, nodes, stigma, apiculus and awns, excluding the pericarp. Notably, the pigmentation in node tissues has not been previously reported for the </span></span><em>OsC1</em> gene, and this gene does not involve in pericarp pigmentation. RT-PCR analysis of transgenic seedlings demonstrated that the overexpression of the <em>OsC1</em> gene upregulated anthocyanin structural genes, particularly <em>OsDFR,</em> leading to anthocyanin accumulation. Intriguingly, the absence of <em>OsB2</em> expression, encoding a MYC transcription factor, in transgenic seedlings suggests the involvement of alternative MYC factors in purple leaf sheaths. This study not only expands our understanding of <em>OsC1</em>'s role in tissue specific anthocyanin pigmentation but also proposes <em>OsC1</em> as a potential visible marker in rice transformation. Utilizing <em>OsC1</em> as a marker provides an alternative approach to address concerns related to antibiotic-resistant genes while providing visually striking pigmentation.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"37 ","pages":"Article 100446"},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139456483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}