Plant Gene最新文献

{"title":"Analysis of marker gene transfer from chloroplasts to mitochondria in heat-shocked and selection-pressured tobacco","authors":"Masaki Odahara ,&nbsp;Maai Mori ,&nbsp;Keiji Numata","doi":"10.1016/j.plgene.2024.100473","DOIUrl":"10.1016/j.plgene.2024.100473","url":null,"abstract":"<div><div>Angiosperm mitochondrial genomes have highly complex and diverse structures that are partly due to frequent insertions of nuclear and chloroplast DNA (cpDNA) into mitochondrial DNA (mtDNA). This suggests the existence of mechanisms for gene transfer from chloroplasts to mitochondria, but these have yet to be discovered. In this study, we aimed to capture chloroplast-to-mitochondrion gene transfer by analyzing the translocation of a marker gene, <em>sul</em>, encoding a bacterial dihydropteroate synthase that confers sulfonamide resistance in tobacco (<em>Nicotiana tabacum</em>), to mtDNA. First, we created tobacco chloroplast transformants in which <em>sul</em>, surrounded on both sides by ∼1 kb of mitochondrial homologous sequences that enable targeted integration into mtDNA, was introduced into the chloroplast genome. Heat shock enhanced <em>sul</em> expression in the transformants, suggesting that chloroplast degradation can stimulate gene transfer from chloroplasts to mitochondria. Shoot regeneration using the heat-shocked chloroplast transformants under sulfadiazine selection resulted in several transformants with moderate resistance to sulfadiazine. Deep sequencing analysis of the target mitochondrial locus detected <em>sul</em> in the sulfadiazine-resistant (SR) plants, but an integration efficiency was 0.0011–0.0051 %. We validated the results by ruling out <em>sul</em> integration into nuclear mitochondrial DNA (NuMT). From these results, we propose the established system is capable of capturing gene transfer from chloroplasts to mitochondria in tobacco, but the transfer efficiency is substantially lower than those from organelles to nucleus.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"40 ","pages":"Article 100473"},"PeriodicalIF":2.2,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142572965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transgressive segregation and generation mean analysis reveal the gene action underlying the inheritance of drought tolerance in rice","authors":"Kossi Lorimpo Adjah ,&nbsp;Maxwell Darko Asante ,&nbsp;Aboubacar Toure ,&nbsp;Mawuli Aziadekey ,&nbsp;Shailesh Yadav ,&nbsp;Felix Frimpong ,&nbsp;Francis Osei Amoako-Andoh ,&nbsp;Daniel Dzorkpe Gamenyah","doi":"10.1016/j.plgene.2024.100472","DOIUrl":"10.1016/j.plgene.2024.100472","url":null,"abstract":"<div><div>Climate change, an effective driver of unprecedented seasonal droughts, is greatly affecting rice production in Africa by threatening food security and safety. Rice, one of the major staple crops on the continent, can save the situation through the development of drought-tolerant cultivars, presenting a major challenge for future rice improvement programs as drought is regarded as a critical limitation in rain-fed ecosystems. This study sought to understand the genetic basis and inheritance behind the expression of tolerance of rice breeding lines to drought-stress through generation mean analysis. To achieve these objectives, two drought-sensitive genotypes (Jasmine 85 and CRI-Agrarice) were crossed with a drought-tolerant genotype (APO) to develop six populations (F₁, F₂, BC₁, BC₂, P₁ and P₂) under screenhouse drought-stress and non-stress evaluation. Data were collected on grain yield and yield-related traits among which the generation mean analysis was conducted. At least one transgressive phenotype was produced in the F₂ population for each trait whether there is a significant difference or not among the parental lines under drought-stress. Under non-stress conditions, there was a significance for all six types of gene action for days to flowering in both crosses. Among both crosses and water-regimes, additive x additive gene interaction was significant for most of the traits even though the scaling tests were not significant indicating the effectiveness of selection in early generations. Therefore, either forward breeding or backcross breeding can be adopted as breeding strategies for rapid improvement for these lines to drought tolerance.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"40 ","pages":"Article 100472"},"PeriodicalIF":2.2,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142530805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted editing of susceptibility genes for plant disease resistance: Current state and future hopes","authors":"Lingareddy Usha Rani ,&nbsp;Manisha Shelke ,&nbsp;Maddi Sandhya ,&nbsp;Govindasamy Senthilraja","doi":"10.1016/j.plgene.2024.100471","DOIUrl":"10.1016/j.plgene.2024.100471","url":null,"abstract":"<div><div>Plants are constantly exposed to a plethora of pathogens including bacteria, fungi, and viruses posing significant challenges to global food security. The susceptibility of plants to these pathogens is often determined by specific genes within their genome. Understanding the role of susceptibility genes in plant-pathogen interactions is crucial for devising effective strategies to combat crop diseases. This review elucidates the importance of susceptibility genes in plants concerning their interactions with fungal, bacterial and viral pathogens. Susceptibility genes often encode proteins involved in crucial cellular processes such as signal transduction, defense response and pathogen recognition. Pathogens exploit vulnerabilities in these genes to establish infection and multiply within the host plant. In addition, advances in genome editing technologies offer promising avenues to enhance plant resistance against pathogens by targeting susceptibility genes. Techniques such as genome editing tools and epigenomic modification allow precise changes to be made in plant genomes, including the elimination or modification of susceptibility genes to confer resistance. However, ethical considerations and regulatory frameworks need to be addressed to ensure the potential use of gene editing in agriculture.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"40 ","pages":"Article 100471"},"PeriodicalIF":2.2,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142530806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and expression analysis of genes encoding late embryogenesis proteins in Cicer arietinum","authors":"Reetu Singh ,&nbsp;Varnika Rana ,&nbsp;Sudesh Kumar Yadav ,&nbsp;Vinay Kumar","doi":"10.1016/j.plgene.2024.100469","DOIUrl":"10.1016/j.plgene.2024.100469","url":null,"abstract":"<div><div>Late embryogenesis abundant (LEA) proteins play defensive roles during seed maturation and seed germination processes. However, there is no such investigation was carried out in chickpea. In present study, genome wide identification and characterization of LEA encoding genes has been investigated, and identified 65 and 74 LEA encoding genes in desi and kabuli cultivar of chickpea, respectively. All these genes have been classified into eight subfamilies on the bases of their phylogenetic analysis and conserved domain. Maximum members of LEA encoding genes were found to be a part of the LEA_2 gene family. The analysis of physicochemical properties of LEAs was also conducted. LEA encoding genes have been found to be located in all chromosomes (8 chr) of chickpea and identified as involved in response to stimulus, biological processes, molecular functions and cellular components based upon gene ontology analysis. Gene expression analysis of randomly selected 8 LEA encoding genes has been carried out during different seed developmental stages which revealed the higher expression of LEA encoding genes during later stage of seed development in chickpea and proved their potential role in desiccation process during seed maturation. During seed germination, expression analysis of LEA encoding genes was found to be higher during the initial stages of seed germination. In conclusion, this work highlights the genome wide identification and characterization of LEA encoding genes in chickpea and proposed potential roles during seed developmental processes. This information could also be useful as a reference investigation for molecular breeding of chickpea for recalcitrant behaviour of seed.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"40 ","pages":"Article 100469"},"PeriodicalIF":2.2,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of clock-associated genes and circadian rhythms in Fragaria × ananassa seedlings","authors":"Misaki Ishibashi ,&nbsp;Norihito Nakamichi ,&nbsp;Yuki Hayashida ,&nbsp;Haruka Kazumori ,&nbsp;Shungo Otagaki ,&nbsp;Shogo Matsumoto ,&nbsp;Akira Oikawa ,&nbsp;Katsuhiro Shiratake","doi":"10.1016/j.plgene.2024.100470","DOIUrl":"10.1016/j.plgene.2024.100470","url":null,"abstract":"<div><div>Flowering time in plants is regulated by a photoperiod-responsive mechanism. Some plant species use a circadian clock-based control mechanism to adapt to variable environments. Strawberry is a horticultural crop that responds to certain photoperiods and temperatures to induce flowering. However, clock-associated genes in octoploid cultivated strawberry (<em>Fragaria × ananassa</em>) have not been defined, and their regulatory mechanism for responding to photoperiods is unclear. We herein targeted 12 clock-associated genes reported in other plant species and performed a genome-wide analysis and expression comparison in <em>F. × ananassa</em> seedlings. Seventy-eight sequences were selected from the <em>F. × ananassa</em> genome. The major domains and <em>cis</em>-acting elements were conserved in each sequence. Transcripts were clearly expressed under continuous light conditions in <em>F. × ananassa</em> seedlings (‘Yotsuboshi’) acclimated to long days. Among them, 9 genes maintained their unique autonomous circadian rhythms and may function as clock genes. LHY (LATE ELONGATED HYPOCOTYL) had the Myb domain and <em>LHY</em> expression peaked in the dawn. PRR (PSEUDO-RESPONSE REGULATOR) family members (<em>PRR9</em>, <em>PRR7</em>, <em>PRR5</em>, and <em>TOC1</em> (<em>TIMING OF CAB EXPRESSION 1</em>)) had a pseudo-receiver domain and CCT domain, and peak expression times began sequentially from the afternoon for <em>PRR9</em> to the evening for <em>TOC1</em>. LUX (LUXARRHYTHMO) had a Myb domain, and <em>LUX</em> expression peaked in evening with <em>ELF3</em> (<em>EARLY FLOWERING 3</em>). FKF1 (<em>FLAVIN-BINDING KELCH REPEAT F BOX 1</em>) had PAS and F-box domains, and <em>FKF1</em> expression peaked in the afternoon. <em>GI</em> (<em>GIGANTEA</em>) expression also peaked in the afternoon. <em>F.</em> × <em>ananassa</em> (‘Yotsuboshi’) appears to have multiple feedback loops comprising clock-associated genes. Although the rhythmic expression of <em>CHE</em> (<em>CCA1 HIKING EXPEDITION</em>) and <em>ZTL</em> (<em>ZEITLUPE</em>) was not observed, they had conserved domains, CHE with the TCP domain and ZTL with the PAS and F-box domains. The present results provide basic information on the circadian clock for the control of <em>F.</em> × <em>ananassa</em> flowering.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"40 ","pages":"Article 100470"},"PeriodicalIF":2.2,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of inflorescence embryogenesis in Festuca Glauca","authors":"Hongjuan Xu ,&nbsp;Baohui Zhang ,&nbsp;Lan Yang ,&nbsp;Yuxuan Jin ,&nbsp;Weize Wang ,&nbsp;Ning Ao ,&nbsp;Panpan Yang ,&nbsp;Zhilin Chen","doi":"10.1016/j.plgene.2024.100468","DOIUrl":"10.1016/j.plgene.2024.100468","url":null,"abstract":"<div><div>In this study<strong>,</strong> RNA-seq was employed for transcriptome sequencing at four developmental stages of normal (L) and embryogenic (X) <em>Festuca glauca</em> ‘Elijah Blue’ inflorescences to analyze and identify the metabolic pathways and regulatory genes associated with inflorescence embryogenesis, thereby facilitating the understanding of the molecular mechanisms of inflorescence embryogenesis in <em>Festuca glauca</em>. The results revealed a total of 50,733 differentially expressed genes (DEGs) between the control (L) and embryogenic (X) samples at different developmental stages. Among them, 19,640 (38.71 %) were upregulated and 31,093 (61.29 %) were downregulated. A total of 2585 DEGs were expressed in both stage 1 (L1-vs-X1) and stage 4 (L4-vs-X4). Gene Ontology (GO) analysis revealed that the DEGs in these stages were mainly enriched in processes related to photosynthetic membranes, chloroplasts, activity of DNA-binding transcription factors, components of ribosomal structure, reactions involving oxidized compounds, and photosynthesis; while Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the DEGs in these stages were mainly enriched in pathways such as plant-pathogen interactions, plant hormone signal transduction, phenylpropanoid biosynthesis, ribosomes, and galactose metabolism. The top families containing differentially expressed transcription factors (DETFs) in these stages included ERF, bHLH, MYB-related, NAC, and WRKY. A total of 39 and 29 DETFs associated with embryogenesis were identified in the L1-vs-X1 and L4-vs-X4 stages, respectively. Additionally, 79 and 110 embryogenesis-related genes were identified in the plant hormone signal transduction metabolic pathway in the L1-vs-X1 and L4-vs-X4 stages, respectively.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"40 ","pages":"Article 100468"},"PeriodicalIF":2.2,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in genome editing and future prospects for Sorghum improvement: A review","authors":"Micheale Yifter Weldemichael ,&nbsp;Hailay Mehari Gebremedhn ,&nbsp;Teklehaimanot Hailesslasie Teklu","doi":"10.1016/j.plgene.2024.100464","DOIUrl":"10.1016/j.plgene.2024.100464","url":null,"abstract":"<div><p>Recent developments in targeted genome editing accelerated genetic research and opened new potentials to improve crops for better yields and quality. Given the significance of cereal crops as a primary source of food for the global population, the utilization of contemporary genome editing techniques like CRISPR/Cas9 is timely and crucial. CRISPR/Cas technology has enabled targeted genomic modifications, revolutionizing genetic research and exploration. Application of gene editing through CRISPR/Cas9 in enhancing sorghum is particularly vital given the current ecological, environmental, and agricultural challenges exacerbated by climate change. As sorghum is one of the main staple foods of our region and known to be a resilient crop with high potential to overcome the above challenges, application of genome editing technology will enhance investigation of gene functionality. CRISPR/Cas9 enables the improvement of desirable sorghum traits, including nutritional value, yield, resistance to pests and diseases, and tolerance to various abiotic stresses. Furthermore, CRISPR/Cas9 has the potential to perform intricate editing and reshape the existing elite sorghum varieties, and introduce new genetic variations. However, current research primarily focuses on improving the efficacy of CRISPR/Cas9 system in successfully editing endogenous sorghum genes, making it a feasible and successful undertaking in sorghum improvement. Recent advancements and developments in CRISPR/Cas9 techniques have further empowered researchers to modify additional genes in sorghum with greater efficiency. Successful application and advancement of CRISPR techniques in sorghum will not only aid in gene discovery, the creation of novel traits that regulate gene expression, and functional genomics, but also in facilitating site-specific integration events. The purpose of this review is, therefore, to elucidate the current advances in sorghum genome editing and highlight its potential in addressing food security issues. It also assesses the efficiency of CRISPR-mediated improvement and its long-term effects on crop improvement and host resistance against parasites, including tissue-specific activity and the ability to induce resistance. This review ends by emphasizing the challenges and opportunities of CRISPR technology in combating parasitic plants, and proposing directions for future research to safeguard global agricultural productivity.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"39 ","pages":"Article 100464"},"PeriodicalIF":2.2,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141840843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular markers that make energy cane differ from sugarcane cultivars (Saccharum spp.)","authors":"Mariana Mancini Benez ,&nbsp;Rone Charles Maranho ,&nbsp;Hugo Zeni Neto ,&nbsp;Claudete Aparecida Mangolin ,&nbsp;Joseli Cristina Silva ,&nbsp;Maria de Fátima Pires da Silva Machado","doi":"10.1016/j.plgene.2024.100465","DOIUrl":"10.1016/j.plgene.2024.100465","url":null,"abstract":"<div><p>Energy cane has been identified as an ideal crop for the sustainable production of biofuels due to its large amounts of lignocellulosic biomass. However, biochemical and molecular characteristics of energy cane have not yet been reported. The current study investigates polymorphism of simple sequence repeats in expressed sequence tags (<em>Est-SSR</em> loci) of energy cane clones PRBIO 172 and PRBIO 130 and of sugarcane varieties CTC 9001, CTC 9003, and RB935744, which permits a direct association between genes for specific proteins and enzymes and traits of agronomic interest. Genetic identity was observed in SSRs associated with loci <em>EstA-68</em> and <em>EstB-130</em> of the three sugarcane varieties and two energy cane clones. The basic contribution of our study was the identification of the polymorphic <em>Est-SRR</em> loci as targets to assess molecular and biochemical divergences of enzymes between sugarcane and energy cane, as well as between the two energy cane clones.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"39 ","pages":"Article 100465"},"PeriodicalIF":2.2,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141688691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the genetic diversity and population structure in Moringa oleifera accessions using DNA markers and phenotypic descriptors","authors":"Preeti Sharma ,&nbsp;Sumita Kachhwaha ,&nbsp;Mahesh Damodhar Mahendrakar ,&nbsp;Shanker Lal Kothari ,&nbsp;Ram Baran Singh","doi":"10.1016/j.plgene.2024.100462","DOIUrl":"https://doi.org/10.1016/j.plgene.2024.100462","url":null,"abstract":"<div><p>Moringa (<em>Moringa oleifera</em> Lam.) is one of the multipurpose trees with significant promise as a high-value crop of industrial importance, having nutritional, therapeutic, and prophylactic properties. Genetic diversity is a cornerstone of any crop improvement program and plays a key role in the selection of promising parental lines for hybrid breeding. Morphological and molecular markers have been proven to be potential tools for the evaluation of genetic diversity, crop genetic improvement, and conservation of plant genetic resources. In the current study, morphological descriptors, RAPD, and SCoT markers were used to determine genetic diversity among 28 <em>M. oleifera</em> accessions. Significant morphological variations were noted for several economic traits across the accessions studied. Four primary clusters were visible on the dendrogram based on phenotypic markers, indicating clustering of accession from a shared geographical habitat. No correlation was estimated between morphological traits, indicating an environmental influence. Three RAPD and seven SCoT primer sets produced 37 and 46 markers, with 53.2 and 71.3% polymorphisms, respectively. Based on genotypic data and the UPGMA approach, all 28 accessions were separated into two major clusters in the phylogenetic tree, irrespective of any geographical areas. The clustering pattern indicates widespread plant species and rapid gene flow through cross-pollination in <em>Moringa</em> populations. Three subpopulations of the involved accessions were identified by population structure analysis; however, there was only a weak link with the location of plant cultivation. The expected heterozygosity for the three subpopulations varied from 0.23 to 0.32, as per R-based structural analysis. AMOVA's attribution of 86% and 19% of all variations to within- and between-populations, respectively, indicates that there has been gene flow across geographic regions. The PCA showed a wide distribution of genotypes in the scatterplot, also suggesting huge genetic variation among the <em>M. oleifera</em> population. The study revealed a significant level of genetic diversity among <em>M. oleifera</em> accessions, which can be harnessed to conserve plant genetic resources and develop high-yielding, nutrient-dense <em>Moringa</em> cultivars.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"39 ","pages":"Article 100462"},"PeriodicalIF":2.2,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141596343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptomic study of matured fruit and post-fruit developmental stages in Malaysian durian varieties","authors":"Siti Zainab Jantan ,&nbsp;Keong Bun Poh ,&nbsp;Florence C. Ginibun","doi":"10.1016/j.plgene.2024.100463","DOIUrl":"https://doi.org/10.1016/j.plgene.2024.100463","url":null,"abstract":"<div><p>Durian (<em>Durio zibethinus</em> Murr.) is a famous tropical fruit in Malaysia and well-known for its sweet and creamy taste and unique strong aroma. Despite the differences, durian fruit undergo similar fruit developmental stages upon maturity. However, not much information related to metabolic changes at molecular level are available for fruit development in durian. Hence, the aim of this study was to identify and analyze fruit development transcriptomic changes on six commercial durian varieties (D24, D99, D160, D168, D197, and D200). The transcriptome analysis via RNA-seq assays generated 67 to 234 million raw reads, which are assembled into 49,601 genes with protein coding genes as the largest gene biotype, with a total of 35,832 genes (72.2%). All genes were annotated against Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). GO analysis revealed genes were highly linked to biological process, cellular components and molecular function, with the highest representation in cell wall, while the most common pathways identified by KEGG were carotenoid biosynthesis, fatty acid biosynthesis, starch and sucrose metabolism, phenylpropanoid biosynthesis and galactose metabolism. Important changes were found in abscisic acid and lignin accumulation, which associated with post-harvest response and concurrent colour change. Moreover, significant increase in butyric acid, palmitoyl-CoA and different forms of sugars were associated with buttery smell, creamy texture, and sweetness respectively. Thus, mass sequence data and expression profiling provide an insight into molecular mechanisms for durian fruit developmental process. This study aims to enhance comprehension of durian fruit development stages, including physiological, genetic, and molecular processes, to inform breeding, crop enhancement, and post-harvest strategies to meet consumer and agro-biotechnology demands.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"39 ","pages":"Article 100463"},"PeriodicalIF":2.2,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141596342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}