Coetaneous activity of Sub1a and SK for maintenance of underwater growth in rice genotypes

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2025-03-26 DOI:10.1016/j.plgene.2025.100502

Sandip Pal , Dip Pal , Rup Kumar Kar , Debasish Panda , Pradip Chandra Dey , Narottam Dey

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Abstract

Despite the availability of flood-tolerant genotypes with Sub1a-mediated elongation, SK has been depicted as the primary gene responsible for elongation under prolonged submergence. However, the combined role of both loci (Sub1a and SK) are prerequisites for the survival of rice plants under flash floods followed by stagnant floods that are yet to be revealed. The combinatorial action of Sub1a and SK in a selected group of lowland rice with the simultaneous presence of both loci were studied for their physio-biochemical performance under induced flash flood followed by water stagnation for 21 days. Among the lines, var. Ganga Sali showed elongation and Kalapatia showed quiescence growth with varied degrees of aerenchyma formation. Further, it was endorsed that var. Ganga Sali showed the highest enzymatic activity of ADH and minimal for PDC under submerged conditions. RT PCR-based expression analysis of Sub1a, SK1, SK2, adh1, pdc1, and susy1 genetic loci under differential submergence showed a mixed response to the depth and duration of the induced flood, among which Sub1a and SK2 showed distinct differences between normal and test plant tissue. From expression analysis, the differential activity of Sub1a and SK2 was recorded, which showed that during the onset of flood, Sub1a starts to express in both the var. Ganga Sali and var. Kalapatia, but as the flood prevails for a longer period, the expression of Sub1a is masked by both SK1 and SK2. Overall, the study demonstrates the combinatorial role of both the genes in two studied rice lines under different flood regimes and their effect on phenotype. The present work may be the first report on the combinatorial expression of Sub1a and SK under differentially induced submergence on a mixed rice population.

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Sub1a和SK在水稻基因型中维持水下生长的同时活性

尽管存在具有sub1a介导的伸长的耐洪水基因型，但SK已被描述为在长时间淹没下负责伸长的主要基因。然而，这两个基因座（Sub1a和SK）的联合作用是水稻在山洪暴发后的滞洪中存活的先决条件，这一点尚未被揭示。选取同时存在Sub1a和SK基因座的水稻为研究对象，研究了这两个基因座在山洪诱导下的生理生化性能。其中Ganga Sali品种表现为伸长，Kalapatia品种表现为静止生长，并形成不同程度的通气组织。此外，在淹没条件下，var. Ganga Sali的ADH酶活性最高，而PDC酶活性最低。基于RT - pcr的差异淹水条件下Sub1a、SK1、SK2、adh1、pdc1和susy1基因位点的表达分析显示，不同淹水条件下，Sub1a、pdc1和susy1基因位点的表达对诱导淹水的深度和持续时间有不同的响应，其中Sub1a和SK2基因位点在正常和受试植物组织中表现出明显差异。从表达分析来看，记录了Sub1a和SK2的差异活性，表明在洪水开始时，Sub1a在Ganga Sali和Kalapatia中都开始表达，但随着洪水持续时间的延长，Sub1a的表达被SK1和SK2掩盖。总之，本研究证明了这两个基因在不同洪水条件下的组合作用及其对表型的影响。本研究可能是首次报道了Sub1a和SK在不同诱导淹没条件下在混合水稻群体上的组合表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.