Molecular marker assisted gene stacking for multiple diseases resistance in an elite rice cultivar, BRRI dhan48

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2025-04-10 DOI:10.1016/j.plgene.2025.100505

Mohammad Abdul Latif, Omar Kayess, Rakibul Hasan, Lutfur Rahman

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引用次数: 0

Abstract

Blast and bacterial blight (BB) pose a significant threat to rice production. To enhance resistance to blast and BB, we employed marker-assisted backcross breeding to introgress the resistance genes into the genetic background of BRRI dhan48. The resistant (R) genes Pi9, Pb1, Xa4, xa13, and Xa21 were introgressed from Pi9-US2, Pb1-US2, and IRBB58, respectively. The Pb1 gene strengthens blast resistance in rice by interacting with WRKY45 transcription factor to modulate salicylic acid and phytoalexin pathways, while the Pi9 gene triggers a signaling cascade involving reactive oxygen species (ROS) production and hormones like jasmonic acid and ethylene activation to enhance defense responses. Xa4 strengthens the cell wall via WAK (cell wall-associated kinase) protein, hindering pathogen invasion, xa13 disrupts SWEET (Sugar Will Eventually be Exported Transporter) protein function to limit sugar availability for Xanthomonas oryzae pv. oryzae (Xoo). Xa21 triggers mitogen-activated protein kinases (MAPKs)-mediated signaling cascades, leading to the activation of WRKY transcription factors that suppress bacterial proliferation. For that, triple cross was made, followed by repeated backcrosses, self-pollinations, and marker-assisted selection to generate BC₃F₅ progeny. Chi-square analysis of 380 BC₃F₂ individuals confirmed a monogenic inheritance pattern for blast and BB resistance traits. Finally, we selected 32 advanced lines, and among them nineteen lines possessed all these 5 R genes, i.e., Pi9, Pb1, Xa4, xa13, and Xa21_, while eight lines consisted of 4 R genes in different combinations. The disease rating of the advanced lines varied from 0 to 5 for both blast and BB diseases, while BRRI dhan48 had a disease rating ranging from 7 to 9. The lines G10, G25, G16, G31, G9, G2, and G20 demonstrated significantly higher grain yield (7.73, 7.72, 7.70, 7.65, 7.64, 7.59, and 7.58 t ha⁻¹, respectively) than the parent (7.00 t ha⁻¹). Investigation of marker trait association showed that molecular markers were negatively linked with blast and BB diseases. So, gene introgression by marker-assisted backcrossing (MABB) offers a robust and efficient way to identify and validate candidate genes with high precision for the development of durable, resistant rice breeding lines. These multiple disease resistant advanced lines may use as a potential genetic stock or incorporate with other desired genes for ensuring sustainable rice production under changing climatic conditions.

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稻瘟病和细菌性枯萎病（BB）对水稻生产构成重大威胁。为了增强对稻瘟病和细菌性白叶枯病的抗性，我们采用了标记辅助回交育种方法，将抗性基因导入 BRRI dhan48 的遗传背景中。抗性（R）基因 Pi9、Pb1、Xa4、xa13 和 Xa21 分别从 Pi9-US2、Pb1-US2 和 IRBB58 中导入。Pb1 基因通过与 WRKY45 转录因子相互作用来调节水杨酸和植物毒素途径，从而增强水稻的抗稻瘟病能力；而 Pi9 基因则触发了一个信号级联，涉及活性氧（ROS）的产生以及茉莉酸和乙烯等激素的激活，从而增强防御反应。Xa4 通过 WAK（细胞壁相关激酶）蛋白强化细胞壁，阻碍病原体入侵；xa13 破坏 SWEET（糖最终将被输出转运体）蛋白功能，限制黄单胞菌（Xanthomonas oryzae pv. oryzae，Xoo）的糖供应。Xa21 触发有丝分裂原激活蛋白激酶（MAPKs）介导的信号级联，从而激活抑制细菌增殖的 WRKY 转录因子。为此，研究人员进行了三系杂交，然后反复进行回交、自花授粉和标记辅助选择，以产生 BC3F5 后代。对380个BC3F2个体的卡方分析证实了抗瘟和抗BB性状的单基因遗传模式。最后，我们选育出 32 个先进品系，其中 19 个品系拥有全部 5 个 R 基因，即 Pi9、Pb1、Xa4、xa13 和 Xa21，8 个品系由不同组合的 4 个 R 基因组成。先进品系的稻瘟病和 BB 病的病害等级从 0 到 5 不等，而 BRRI dhan48 的病害等级从 7 到 9 不等。品系 G10、G25、G16、G31、G9、G2 和 G20 的粮食产量（分别为 7.73 吨/公顷、7.72 吨/公顷、7.70 吨/公顷、7.65 吨/公顷、7.64 吨/公顷、7.59 吨/公顷和 7.58 吨/公顷）明显高于母本（7.00 吨/公顷）。标记性状关联调查表明，分子标记与稻瘟病和 BB 病呈负相关。因此，通过标记辅助回交（MABB）进行基因导入，为高精度鉴定和验证候选基因提供了一种稳健而有效的方法，可用于培育持久抗病的水稻育种品系。这些具有多重抗病性的先进品系可作为潜在的基因储备，或与其他所需基因结合，以确保在不断变化的气候条件下实现水稻的可持续生产。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.