Genome-wide association of root growth angle, seminal root numbers, and leaf area in the synthetic hexaploid wheat diversity panel

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2025-04-11 DOI:10.1016/j.plgene.2025.100507

Maria Khalid , Zoya Khalid , Zubair Ahmed , Lee Hickey , Awais Rasheed , Alvina Gul , Rabia Amir

{"title":"Genome-wide association of root growth angle, seminal root numbers, and leaf area in the synthetic hexaploid wheat diversity panel","authors":"Maria Khalid , Zoya Khalid , Zubair Ahmed , Lee Hickey , Awais Rasheed , Alvina Gul , Rabia Amir","doi":"10.1016/j.plgene.2025.100507","DOIUrl":null,"url":null,"abstract":"<div><div>A lack of understanding about the variations in root number, root angle, and leaf area contributes to inability of wheat withstand drought stress during seedling stage. This discrepancy hinders the development of drought-tolerant wheat varieties. We aimed to evaluate germplasm at seedling stage to find association between markers and traits. We characterized a collection of synthetic hexaploid wheat for root angle, seminal root numbers, and early vigor and identified loci associated with these traits using a 50 K SNP array and genotyping-by-sequencing (GBS) platforms. In total, 39 marker-trait nucleotides (QTNs) were associated with LA, RA, and RN distributed over 10 wheat chromosomes. Out of these, 11 QTNs were associated with LA, 15 QTNs were associated with RA, and 13 QTNs were associated with RN.LA showed a negative correlation with RN and a poor correlation with RA. The phenotypic values of synthetic hexaploid wheat accessions with a higher frequency of favorable alleles have been found to increase. Therefore, prioritizing the use of these synthetic wheat varieties in wheat breeding programs can be beneficial for improving root system architecture (RSA) and early vigor. These preliminary findings can be useful for pre-breeding efforts aimed at enhancing wheat adaptability to drought stress conditions.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100507"},"PeriodicalIF":2.2000,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Gene","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352407325000186","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}

引用次数: 0

Abstract

A lack of understanding about the variations in root number, root angle, and leaf area contributes to inability of wheat withstand drought stress during seedling stage. This discrepancy hinders the development of drought-tolerant wheat varieties. We aimed to evaluate germplasm at seedling stage to find association between markers and traits. We characterized a collection of synthetic hexaploid wheat for root angle, seminal root numbers, and early vigor and identified loci associated with these traits using a 50 K SNP array and genotyping-by-sequencing (GBS) platforms. In total, 39 marker-trait nucleotides (QTNs) were associated with LA, RA, and RN distributed over 10 wheat chromosomes. Out of these, 11 QTNs were associated with LA, 15 QTNs were associated with RA, and 13 QTNs were associated with RN.LA showed a negative correlation with RN and a poor correlation with RA. The phenotypic values of synthetic hexaploid wheat accessions with a higher frequency of favorable alleles have been found to increase. Therefore, prioritizing the use of these synthetic wheat varieties in wheat breeding programs can be beneficial for improving root system architecture (RSA) and early vigor. These preliminary findings can be useful for pre-breeding efforts aimed at enhancing wheat adaptability to drought stress conditions.

查看原文本刊更多论文

合成六倍体小麦多样性面板中根生长角、种子根数和叶面积的全基因组关联

由于对根系数量、根角和叶面积的变化缺乏了解，小麦在苗期无法抵御干旱胁迫。这种差异阻碍了耐旱小麦品种的发展。我们的目的是在苗期对种质进行评价，寻找标记与性状之间的关联。研究人员利用50 K SNP阵列和基因分型测序（GBS）平台对合成六倍体小麦的根角、种子根数和早期活力进行了表征，并确定了与这些性状相关的位点。共有39个与LA、RA和RN相关的标记性状核苷酸（QTNs）分布在10条小麦染色体上。其中，11个qtn与LA相关，15个qtn与RA相关，13个qtn与RN相关。LA与RN呈负相关，与RA相关性较差。有利等位基因频率较高的合成六倍体小麦材料的表型值增加。因此，在小麦育种计划中优先使用这些合成小麦品种有利于改善根系结构（RSA）和早期活力。这些初步发现可用于提高小麦对干旱胁迫条件的适应性的育种工作。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.