Jornal Brasileiro de Patologia e Medicina Laboratorial最新文献_第7页

Quantitative and qualitative evaluation of IgG synthesis in 8,947 cerebrospinal fluid samples 8,947例脑脊液IgG合成的定量和定性评价

Jornal Brasileiro de Patologia e Medicina Laboratorial Pub Date : 2020-01-01 DOI: 10.5935/1676-2444.20200004

R. Domingues, G. Peres, Fernando B. Moura-Leite, C. S. Soares

{"title":"Quantitative and qualitative evaluation of IgG synthesis in 8,947 cerebrospinal\u0000 fluid samples","authors":"R. Domingues, G. Peres, Fernando B. Moura-Leite, C. S. Soares","doi":"10.5935/1676-2444.20200004","DOIUrl":"https://doi.org/10.5935/1676-2444.20200004","url":null,"abstract":"Introduction: Increased intrathecal immunoglobulin class G (IgG) synthesis can be found in several neuroinflammatory diseases. Objective: The aim of this study was to analyze quantitative and qualitative methods of intrathecal immunoproduction evaluation. Methods: We retrospectively assessed data from cerebrospinal fluid (CSF) and serum samples sent to Senne Liquor Diagnóstico from 2001 to 2017. Cytological, biochemical, and immunological data were compared between cases with and without oligoclonal bands (OCBs). Comparisons between samples with OCBs (OCB+) and without them (OCB-) were carried out, and the ability to predict the presence of OCBs was assessed with ROC analysis. Results: We included 8,947 samples (2,599 OCB+ samples and 6,348 OCBsamples). CSF lymphocytes and monocytes were significantly associated with the presence of OCB (p < 0.001). All the inflammatory parameters were significantly associated with OCB, and the methods with the highest predictive ability for OCB were IgG index and Reiber diagram [area under the receiver operating characteristic (AUROC) curve = 0.881 and 0.863, respectively]. A small percentage of cases without OCB had high IgG index (9.56%) or Reiber diagram, showing increased IgG production (4.6%). Conclusion: We showed a strong association between OCB and other CSF inflammatory parameters. The presence of cases with increased quantitative CSF IgG synthesis without OCB suggests that both quantitative and qualitative methods should be performed in the evaluation of neuroinflammatory processes.","PeriodicalId":35397,"journal":{"name":"Jornal Brasileiro de Patologia e Medicina Laboratorial","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71115918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of hemosiderotic adenodermatofibroma in Brazil 巴西首例含铁血黄素性腺皮纤维瘤报告

Jornal Brasileiro de Patologia e Medicina Laboratorial Pub Date : 2020-01-01 DOI: 10.5935/1676-2444.20200023

M. A. Pereira, L. Pantaleão, M. Rochael

引用次数: 1

Fascin overexpression in nasal mucosa in patients with chronic rhinosinusitis with and without nasal polyps 伴有和不伴有鼻息肉的慢性鼻窦炎患者鼻黏膜中筋膜蛋白的过表达

Jornal Brasileiro de Patologia e Medicina Laboratorial Pub Date : 2020-01-01 DOI: 10.5935/1676-2444.20200007

A. Tkachenko, G. Gubina-Vakulyck, A. Onishchenko, I. Kalashnyk, Ya. O. Butko, T. Gorbach

引用次数: 0

Avaliação de variáveis pré-analíticas em exames laboratoriais de pacientes atendidos no Laboratório Central de Vitória da Conquista, Bahia, Brasil 在巴西巴伊亚州vitoria da Conquista中心实验室接受治疗的患者的实验室检查中分析前变量的评估

Jornal Brasileiro de Patologia e Medicina Laboratorial Pub Date : 2020-01-01 DOI: 10.5935/1676-2444.20200009

Ludimila Rocha Ramos, Márcio Vasconcelos Oliveira, Cláudio Lima Souza

引用次数: 3

Evaluation of PSA requests in men under 40 years of age 40岁以下男性PSA要求的评估

Jornal Brasileiro de Patologia e Medicina Laboratorial Pub Date : 2020-01-01 DOI: 10.5935/1676-2444.20200021

F. Araújo, L. A. Bittencourt, N. Sumita, U. Barroso

引用次数: 1

Oral glucose tolerance test: unnecessary requests and suitable conditions for the test 口服葡萄糖耐量试验:不必要的要求和适宜的试验条件

Jornal Brasileiro de Patologia e Medicina Laboratorial Pub Date : 2020-01-01 DOI: 10.5935/1676-2444.20200010

Gabriele A. Silva, Cláudio Lima Souza, Márcio Vasconcelos Oliveira

{"title":"Oral glucose tolerance test: unnecessary requests and suitable conditions for the\u0000 test","authors":"Gabriele A. Silva, Cláudio Lima Souza, Márcio Vasconcelos Oliveira","doi":"10.5935/1676-2444.20200010","DOIUrl":"https://doi.org/10.5935/1676-2444.20200010","url":null,"abstract":"Introduction: The oral glucose tolerance test (OGTT) is an important test for diagnosis of diabetes mellitus (DM) that needs criteria to be requested and analytical performance evaluation. Objectives: Determine the prevalence of unnecessary OGTT requests and analyze patients’ suitability criteria for glucose load. Method: Cross-sectional, descriptive and analytical study conducted with 554 patients who had OGTT requested from January to April 2018. Data from laboratory tests were collected through Complab Advanced version 6.9.6 system, organized into a Microsoft Excel table and analyzed using Epi Info, version 7.2.1.0. The accuracy of the glucometer paired analysis was performed by determinations of Student’s t test, using SPSS version of IBM 21. Patients with a previous diagnosis of DM and/or who showed OGTT request as a screening test along with blood glucose or glycated hemoglobin were classified as unnecessary requests. Results: Among the studied patients, 17% (94) had unnecessary OGTT requests, 53 (53.4%) patients had blood glucose ≥ 140 mg/dl and previous DM and/or OGTT used as screening; 41 (43.6 %) patients with capillary blood glucose < 140 mg/dl, but with a diagnosis of DM. The glucometer proved to be accurate with a high correlation (r = 0.97, p < 0.0001) with serum glucose. Approaches during screening and capillary blood glucose prevented unnecessary exposure to glucose overload in 67% (63) of the patients. Conclusion: The high prevalence of unnecessary OGTT requests underscores the need of criteria for OGTT requesting and the standardization of procedures for screening in the exam.","PeriodicalId":35397,"journal":{"name":"Jornal Brasileiro de Patologia e Medicina Laboratorial","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71116529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Performance analysis of commercial rapid tests for serum troponin detection 商用血清肌钙蛋白快速检测试剂盒的性能分析

Jornal Brasileiro de Patologia e Medicina Laboratorial Pub Date : 2020-01-01 DOI: 10.5935/1676-2444.20200020

Raquel Ida Ferreira, Marcela R. Amaral, Maytê S. R. Brito, Matheus P. Pinto, Ana Carolina R. Maranhão, Lucas Costa, Rómulo Melo

{"title":"Performance analysis of commercial rapid tests for serum troponin detection","authors":"Raquel Ida Ferreira, Marcela R. Amaral, Maytê S. R. Brito, Matheus P. Pinto, Ana Carolina R. Maranhão, Lucas Costa, Rómulo Melo","doi":"10.5935/1676-2444.20200020","DOIUrl":"https://doi.org/10.5935/1676-2444.20200020","url":null,"abstract":"Introduction: Rapid tests represent an important diagnostic tool, providing results in a short period of time and eliminating the use of large automated equipment. Objective: To evaluate the performance of five rapid test kits for troponin I detection in serum. Materials and methods: Samples from 100 patients with suspected acute myocardial infarction (AMI) were selected from a hospital in Barbacena, MG, Brazil. They were tested in the five troponin I commercial rapid test kits and the results were compared with the study reference method (quantitative chemiluminescent immunoassay). Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and correlation coefficients of the rapid tests against the reference method were defined. Results: The kits from Abon ® , Interteck ® , Medtest ® and Wama ® presented the same performance, reaching equal levels of sensitivity (98.08%), specificity (100%), PPV (100%), NPV (97.96%), and correlation coefficient (0.99). The Eco Diagnostica ® kit presented lower sensitivity (82.69%), low correlation coefficient (0.91) and NPV (84.21%), not reaching ideal levels even though lying within the confidence interval. Discussion: The results obtained with the Abon ® , Interteck ® , Medtest ® and Wama ® test kits corroborate pre-existing literature data on the diagnostic accuracy of rapid tests for troponin detection. These tests are allowed for immediate decision making by physicians and can be used to reduce unnecessary hospitalization time and costs associated with a suspected AMI. Conclusion: The kits from Abon ® , Interteck ® , Medtest ® and Wama ® showed excellent diagnostic performance, what makes them an important diagnostic tool in smaller laboratories without automated equipment.","PeriodicalId":35397,"journal":{"name":"Jornal Brasileiro de Patologia e Medicina Laboratorial","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71116549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Receptores de progesterona e estradiol e Ki-67 no estroma e no epitélio de endometriose superﬁcial e profunda 浅部和深层子宫内膜异位症间质和上皮中的孕酮、雌二醇和Ki-67受体

Jornal Brasileiro de Patologia e Medicina Laboratorial Pub Date : 2020-01-01 DOI: 10.5935/1676-2444.20200014

Luiz Ferraz de Sampaio Neto, Maria Cecília Ferro, L. D. Garcia, B. Ribeiro

{"title":"Receptores de progesterona e estradiol e Ki-67 no estroma e no epitélio de endometriose superﬁcial e profunda","authors":"Luiz Ferraz de Sampaio Neto, Maria Cecília Ferro, L. D. Garcia, B. Ribeiro","doi":"10.5935/1676-2444.20200014","DOIUrl":"https://doi.org/10.5935/1676-2444.20200014","url":null,"abstract":"Introduction: Endometriosis is a hormone-dependent disease characterized by ectopic presence of endometrial tissue responsive to ovarian steroids. Estrogen and progesterone are the main regulators of endometrial tissue, and the expression of receptors of these hormones in the ectopic tissue seems to be related to the pathophysiology of the disease. Ki-67 is a marker of tissue proliferation and an important marker of epithelial kinetics. Endometriosis can be classiﬁed as superﬁcial, in the peritoneum, and deep, when it extends into ligaments and other organs. Objective: Our objective was to analyze the expression of estrogen and progesterone receptors and Ki-67, through immunohistochemistry, in different sites of endometriosis tissues (superﬁcial peritoneal/ovarian endometriosis and deep inﬁltrating endometriosis). Casuistic and methods: We studied nine patients; ﬁve with superﬁcial and four with deep endometriosis. Statistical correlation was performed with the Shapiro-Wilk test (signiﬁcance level of 5%) and linear correlation analysis using Spearman’s non-parametric test (signiﬁcance of 1%). There was a correlation of Spearman between the estrogen receptor variable and Ki-67 in patients with superﬁcial endometriosis. There was also a correlation between the variables estrogen receptor and progesterone receptor in patients with deep endometriosis. Results: Contrary to what was found for superﬁcial endometriosis, there is linear increase of the variables, with a strong and positive correlation coefﬁcient. This demonstrates that the variation of estrogen receptors can be explained in 99.1% by the same variation of progesterone receptors in deep endometriosis. Conclusion: It is possible to infer that other factors are involved in the response to hormonal variations for superﬁcial and deep endometriosi s.","PeriodicalId":35397,"journal":{"name":"Jornal Brasileiro de Patologia e Medicina Laboratorial","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71116173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of HLA-G immune checkpoint molecule in melanocytic lesions HLA-G免疫检查点分子在黑色素细胞病变中的评价

Jornal Brasileiro de Patologia e Medicina Laboratorial Pub Date : 2020-01-01 DOI: 10.5935/1676-2444.20200016

R. P. Gomes, Gabriela Tayrine Pereira Caetano, L. H. Sampaio, Camila R Silva, M. B. Costa, Maria Auxiliadora Cysneiro, I. Wastowski

引用次数: 0

Granular cell tumor in female neonate: a case report 女性新生儿颗粒细胞瘤1例

Jornal Brasileiro de Patologia e Medicina Laboratorial Pub Date : 2020-01-01 DOI: 10.5935/1676-2444.20200019

Henrique M. Pasqual, Andreza Maldaner, Maiara Christine Macagnan, Rafaela A. Link, N. B. Corrêa, R. S. Borges, Vitor B. Santana

引用次数: 0