Plant Stress最新文献

{"title":"Transcriptome analysis revealed the molecular mechanism of the response to leaf spot disease in Camellia sasanqua","authors":"Hongye Zhang,&nbsp;Cheng Yang,&nbsp;Lei Zong,&nbsp;Longqing Chen,&nbsp;Tian Wu","doi":"10.1016/j.stress.2025.101015","DOIUrl":"10.1016/j.stress.2025.101015","url":null,"abstract":"<div><div>The pathogens on the leaves of the <em>Camellia sasanqua</em> seriously affected the ornamental value. Illuminating the underlying molecular mechanisms is of extraordinary importance in <em>C. sasanqua</em> to improve resistance variety. We isolated the pathogens of <em>Fusarium sambucinum, Alternaria alternata, Phyllosticta capitalensis</em>, and <em>Diaporthe amygdali</em> from the diseased leaves of <em>C. sasanqua.</em> These four pathogens might cause the leaf spot of <em>C. sasanqua</em> by working together. The transcriptomic analysis detected differences between healthy and diseased leaf samples of <em>C. sasanqua</em>, and 8139 DEGs were identified, including 4544 up-regulated genes and 3595 down-regulated genes. KEGG enrichment analysis of the canonical defensive pathways and the genes with high expression levels indicated that the response of <em>C. sasanqua</em> to pathogens was a complex signal network, including signal recognition and transmission, plant hormones including SA and ABA, activation of transcription factors of MYB, AP2/ERF, WRKY, and secondary metabolic accumulation. The genes of the phenylpropanoid biosynthesis pathway were expressed significantly in the response processes. We further cloned the genes of <em>CAD</em> (Cinnamic alcohol dehydrogenase) and <em>COMT</em> (Caffeic acid 3-O-methyltransferase), respectively, in the phenylpropanoid biosynthesis pathway, and then characterized their functions in tobacco. The results showed that overexpressing <em>CsCAD5</em> and <em>CsCOMT1</em> could influence the accumulation of lignins. We speculated that the increased lignin content in plants might be achieved by thickening and lignifying the cell wall. In summary, our findings discussed the complexities and interactions of <em>C. sasanqua</em> responses to leaf spot, identifying potential resistance genes and molecular mechanisms for preventing and controlling plant diseases.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101015"},"PeriodicalIF":6.8,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of the UGT gene family revealing PbUGT73EC3 participating in drought stress in Phoebe bournei","authors":"Yan Liu ,&nbsp;Qinglin Sun ,&nbsp;Qiguang Wang ,&nbsp;Hao Wu ,&nbsp;Yuting Zhang ,&nbsp;Chunlong Li ,&nbsp;Dong Meng ,&nbsp;Junhong Zhang ,&nbsp;Zaikang Tong","doi":"10.1016/j.stress.2025.101012","DOIUrl":"10.1016/j.stress.2025.101012","url":null,"abstract":"<div><div>Drought stress significantly limits plant growth and development, and previous studies have demonstrated that glycosyltransferases play crucial roles in stress responses. However, the mechanisms by which <em>UGT</em> genes confer drought tolerance in <em>Phoebe bournei</em> remain largely unknown. In this study, a total of 151 <em>PbUGT</em> genes were identified and systematically named. Phylogenetic analysis classified these genes into 22 subfamilies, all of which contain the conserved PSPG box. Cis-element analysis identified a large number of stress-responsive elements in the promoters of <em>PbUGT</em> members. Combined transcriptomic and RT-qPCR analyses showed that the majority of members from the <em>UGT73, UGT74, UGT85, UGT89</em>, and <em>UGT707</em> subfamilies were highly expressed under PEG-induced drought conditions. Moreover, nine candidate genes exhibited significant transcriptional responses to drought stress. <em>PbUGT73EC3</em>, in particular, was selected for functional validation. In all three transgenic systems, its overexpression significantly increased the accumulation of kaempferol-3-<em>O</em>-rutinoside. The <em>PbUGT73EC3-</em>overexpressing lines displayed enhanced drought tolerance, with increased flavonoid content, elevated activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), and reduced levels of malondialdehyde (MDA), hydrogen peroxide (H₂O₂), and leaf water loss rate. These results suggest that <em>PbUGT73EC3</em> may enhance plant drought tolerance by regulating the biosynthesis of kaempferol-3-O-rutinoside and promoting the scavenging of reactive oxygen species (ROS) through glycosylation. In summary, these findings provide a comprehensive characterization of the <em>UGT</em> gene family in <em>P. bournei</em> and elucidate their potential roles in drought stress responses, offering a theoretical basis for molecular breeding aimed at improving drought tolerance and for further functional studies of <em>UGT</em> genes.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101012"},"PeriodicalIF":6.8,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144906931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of the Spanish fir transcriptome in nature: Metabolic pathways and gene networks involved in the response to climate stress","authors":"Irene Blanca-Reyes ,&nbsp;María Torés-España ,&nbsp;Victor Lechuga ,&nbsp;María Teresa Llebrés ,&nbsp;Fernando N. de la Torre ,&nbsp;José A. Carreira ,&nbsp;Concepción Avila ,&nbsp;Francisco M. Cánovas ,&nbsp;Vanessa Castro-Rodríguez","doi":"10.1016/j.stress.2025.101009","DOIUrl":"10.1016/j.stress.2025.101009","url":null,"abstract":"<div><div>Circum-Mediterranean firs, and particularly the relic Spanish-fir from the south of the Iberian Peninsula (<em>Abies pinsapo</em> Boiss.), are among the most drought-sensitive and vulnerable to climate change within the broad context of Mediterranean forest species. Forest decline and die-back episodes associated to warming trends and recurrent droughts of increasing duration and intensity in the last decades point to an increasing vulnerability of <em>A. pinsapo</em> local populations. In this work, physiological and transcriptional analyses were combined to assess the response of trees growing in natural forests under contrasting conditions. The results show a modulation of the transcriptome in response to climatic stress with substantial changes in the expression of genes involved in water stress, aromatic amino acid metabolism, and transcription factors associated with the transcriptional regulation of the observed patterns of gene expression. Roots were determined to be the primary organs involved in the transcriptional response to stress, which may be mediated by a gene-network including interactions among structural and regulatory genes. Interactive elements comprise genes encoding stress-related proteins of the ApLEA family, ApADH, the first committed enzyme in tyrosine biosynthesis, and root-specific transcription factors implicated in stress regulation belonging to the ApERF, ApYABBY, and ApNAC superfamilies. Data provide new insights to understand the response of Spanish firs to current climatic pressure by the identification of individual genes and gene-networks potentially involved in local adaptation. This new knowledge will facilitate comparative studies of gene variation in the Spanish fir populations using the identified genes as molecular markers for the selection of the best adapted genotypes in the response to climate stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101009"},"PeriodicalIF":6.8,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145003915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High radiosensitivity in the conifer Norway spruce (Picea abies) due to less comprehensive mobilisation of protection and repair responses compared to the radiotolerant Arabidopsis thaliana","authors":"Payel Bhattacharjee ,&nbsp;Dajana Blagojevic ,&nbsp;YeonKyeong Lee ,&nbsp;Gareth B Gillard ,&nbsp;Lars Grønvold ,&nbsp;Torgeir Rhoden Hvidsten ,&nbsp;Simen Rød Sandve ,&nbsp;Ole Christian Lind ,&nbsp;Brit Salbu ,&nbsp;Dag Anders Brede ,&nbsp;Jorunn E. Olsen","doi":"10.1016/j.stress.2025.101010","DOIUrl":"10.1016/j.stress.2025.101010","url":null,"abstract":"<div><div>Risk assessment and protection of plant communities in contaminated ecosystems require in-depth understanding of differential sensitivity to chronic ionising radiation in plants. However, the contributing molecular factors to differential radiosensitivity among plant species are poorly understood. To shed light on this, we compared early events associated with protection, repair, and stress responses in gamma-irradiated (1–290 mGy h^-1) seedlings of the radiosensitive conifer Norway spruce (<em>Picea abies</em>) and the radiotolerant <em>Arabidopsis thaliana</em>, by analysing growth, organelle and DNA damage, transcriptomes and the dynamics of antioxidant activities and expression of relevant genes. After 48 h of gamma radiation exposure, Norway spruce showed significantly reduced growth at 100–290 mGy h^-1 and organelle damage, especially in mitochondria, at ≥ 1 mGy h^-1 whereas <em>A. thaliana</em> showed normal vegetative growth at all dose rates, transiently delayed reproductive development at 290 mGy h^-1 only, minor organelle damage only at ≥ 100 mGy h^-1 and significantly less DNA damage than in Norway spruce at all dose rates. Comparative transcriptomics revealed that <em>A. thaliana</em> showed massive activation of genes related to DNA damage repair, antioxidants, and other stress responses at ≥ 1 mGy h^-1 while Norway spruce mobilized transcription of such pathways only at ≥ 40 mGy h^-1. The transcriptional activation of repair and protection responses at higher gamma dose-rates only and its absence in lower dose-rates, correlates with high radiosensitivity of Norway spruce, compared to the massive transcriptional activation from low dose-rates in the radiotolerant <em>A. thaliana.</em></div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101010"},"PeriodicalIF":6.8,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144920424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GABA is a key player regulating the TCA cycle and polyamine metabolism under combined heat-drought stress in tea plants","authors":"Qinqin Gao ,&nbsp;Deng Deng ,&nbsp;Rou Zeng ,&nbsp;Yun Liu ,&nbsp;Jie Jiang ,&nbsp;Qiang Shen ,&nbsp;Yuanchun Ma ,&nbsp;Wanping Fang ,&nbsp;Xujun Zhu","doi":"10.1016/j.stress.2025.101006","DOIUrl":"10.1016/j.stress.2025.101006","url":null,"abstract":"<div><div>Throughout their development, plants experience a range of abiotic stresses, typically not solitary occurrences. For example, drought stress (DS) and heat stress (HS) often co-occur due to a high-temperature environment being accompanied by drought. Differing from single stress, plants have unique responses to the stress combination, with secondary metabolism holding a pivotal position in the process of plant response. Under combined stresses, plants specifically induce the accumulation of secondary metabolites to resist damage. We found that the metabolic responses of tea plants (<em>Camellia sinensis</em>) to DS or HS differed from those to a combination of HS and DS (HS-DS). Metabolic analysis showed that combined HS-DS led to the up-regulation and down-regulation of abundance of key metabolites in the tricarboxylic acid (TCA) cycle and polyamine metabolism pathways. Among the metabolites accumulated under combined HS-DS was γ-aminobutyric acid (GABA). Exogenous spraying of 1 mM GABA and silencing the GABA-synthesis-related gene [glutamate decarboxylase 1 (<em>GAD1</em>)] showed that GABA played a crucial part in the resistance of tea plants to combined HS-DS. This study reveals the function of GABA in regulating the response of tea plant to HS-DS, which provides a theoretical basis for the subsequent research on heat and drought resistance for plants.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101006"},"PeriodicalIF":6.8,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial roles in sodium detoxification and silicon bioavailability for improved soil quality and plant tolerance and productivity","authors":"Qian Xu ,&nbsp;Pengfei Li ,&nbsp;Yuxin Lu ,&nbsp;Zhizhen Feng ,&nbsp;Hong Yan ,&nbsp;Wenjuan Zhao ,&nbsp;Tao Qin ,&nbsp;Wei He ,&nbsp;Jun Zhang ,&nbsp;Penghui Zhang","doi":"10.1016/j.stress.2025.101001","DOIUrl":"10.1016/j.stress.2025.101001","url":null,"abstract":"<div><div>Global agricultural output is severely hampered by soil salinity and abiotic factors including sodium (Na⁺) toxicity. Recent studies have highlighted the importance of helpful bacteria in reducing these pressures. In order to increase soil quality and plant production, this review focuses on two particular microorganisms, <em>Arthrobacter</em> sp. and <em>Bacillus mucilaginosus</em>, and their processes for improving silicon (Si) bioavailability and detoxifying sodium. <em>Arthrobacter</em> sp. is well-known for its capacity to break down organic compounds and generate biosurfactants that change the chemistry of soil and lessen the build-up of salt. This bacterium improves water infiltration, aeration, and soil aggregation—all of which are essential for reducing sodium toxicity. <em>Arthrobacter</em> sp. also produces organic acids that chelate sodium ions, keeping them from being absorbed by plant roots and lessening their harmful effects. Conversely, <em>Bacillus mucilaginosus</em> is excellent at solubilizing silicon compounds, increasing its accessibility for plants. It generates the organic acids and enzymes needed to decompose silicate minerals and release soluble silicon into the soil. Plants with higher Si absorption are more resilient to abiotic stressors such as drought, salinity, and heavy metal toxicity, which promotes better growth and less damage from stress. Using <em>Arthrobacter</em> sp. and <em>Bacillus mucilaginosus</em> in tandem offers a viable way to improve crop productivity and soil quality. These microbial treatments have been shown in field experiments to increase crop yields in saline areas, supporting sustainable farming methods. Clarifying the molecular mechanisms underlying these bacteria's actions, such as the function of organic acids and microbial enzymes in mineral solubilization and the control of stress-related genes in plants, should be the main goal of future research. An eco-friendly way to counteract the negative effects of salt and other abiotic stresses on crops is to create microbial consortia that combine the advantages of different beneficial bacteria. This could further improve soil health and agricultural resilience.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101001"},"PeriodicalIF":6.8,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic insights into the symbiotic relationships and functional roles of bark microbiomes in tea trees","authors":"Lijiao Chen ,&nbsp;Xianyao Li ,&nbsp;Limei Li ,&nbsp;Gelan Wang ,&nbsp;Siqin Chen ,&nbsp;Miao Wang ,&nbsp;Xuemei Zhang ,&nbsp;Zejun Wang ,&nbsp;Zihua Zhao ,&nbsp;Quanyuan Xin ,&nbsp;Zhengmei Chen ,&nbsp;Yuan Zhao ,&nbsp;Bingbing Jiang ,&nbsp;Baijuan Wang ,&nbsp;Ming Zhao","doi":"10.1016/j.stress.2025.101007","DOIUrl":"10.1016/j.stress.2025.101007","url":null,"abstract":"<div><div>Bark represents a fickle microbial habitat that is significantly influenced by environmental factors, tissue age, and etc. The permanent surface of <em>Camellia sinensis</em> bark exhibits the mosaic plaques resulting from the presence of epiphytic microorganisms, particularly on ancient trees. Investigation of microbiome focus on harvested parts of tea tree, but there remains a lack of understanding the associated bark microbial communities. This is significant, as the bark serves as a crucial link between the above-ground harvested components and the subterranean parts of the plant. Here, we utilized amplicon sequencing, microbial isolation and phenotype observation to examine the bark microbiomes of tea trees located on sunny and shady slopes in one grove. Additionally, we conducted metagenomic sequencing, assembly, and binning to explore the potential functions of microorganisms. Comparative analysis revealed significant differences in microbial taxa between epiphytic plaques of individual trees and in slope-associated community structures between the two trees. Co-occurrence networks, characterized by hub nodes of lichenicolous microorganisms, demonstrated positive symbiotic relationships among pathogenic and beneficial microbes. Moreover, the abundance of genes related to carbohydrate and amino acid production in the metagenome-assembled genomes (MAGs) of Acetobacteraceae suggests a potential role in carbon and nitrogen cycles. The genes involved in substance and signal exchange further support the stable symbiont. This study will serve as a valuable resource for guiding future research on utilizing bark microbial resources and genes of primary productivity for agronomical purposes.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101007"},"PeriodicalIF":6.8,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145060463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the multifaceted role of C2H2-type zinc finger proteins: integrators of trichome development and abiotic stress adaptation in plants","authors":"Muhammad Aneeq Ur Rahman ,&nbsp;Muhammad Ali Abid ,&nbsp;Zoya Batool Naqvi ,&nbsp;Mubashir Abbas ,&nbsp;Waqas Malik ,&nbsp;Yunxiao Wei ,&nbsp;Rui Zhang","doi":"10.1016/j.stress.2025.101005","DOIUrl":"10.1016/j.stress.2025.101005","url":null,"abstract":"<div><div>C2H2-type zinc finger proteins function as important transcriptional regulators in plants, facilitating the integration of developmental processes and environmental stress responses. This review demonstrated a comprehensive understanding of the classification, structure, and functional domains of C2H2-type zinc finger proteins, with particular emphasis on their dual roles in trichome development and abiotic stress adaptation. We described the role of C2H2-type zinc finger proteins in regulating unicellular and multicellular trichome formation in <em>Arabidopsis</em> and other plant species. Furthermore, the review summarized the role of these proteins in mediating plant responses to diverse abiotic stresses, including drought, oxidative, cold, heat and salinity stress. Under drought conditions, C2H2 type zinc finger proteins are known to enhance water retention and antioxidant activity; whereas during salt stress, they modulate the expression of protective genes and maintain ionic balance. Additionally, the interplay between C2H2-type zinc finger proteins and phytohormones, such as ABA and auxin, elucidated their integral role in stress signaling networks. This review explored into the molecular cross-talk between trichome development and stress response pathways, underscoring the versatility and regulatory complexity of C2H2-type zinc finger proteins. Unraveling the molecular mechanisms by which C2H2-type zinc finger proteins improve trichome development and enhance stress tolerance will facilitate the breeding and genetic engineering of crops with improved traits.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101005"},"PeriodicalIF":6.8,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-mediated editing of uORFs in the BnVTC2 facilitates abiotic stress resilience without yield penalty","authors":"Mengyu Hao ,&nbsp;Yilin Li ,&nbsp;Shifei Sang ,&nbsp;Miaoying Song ,&nbsp;Yunfei Wen ,&nbsp;Hui Wang ,&nbsp;Wenxiang Wang ,&nbsp;Desheng Mei ,&nbsp;Jia Liu ,&nbsp;Chao Li ,&nbsp;Li Fu ,&nbsp;Qiong Hu ,&nbsp;Hongtao Cheng","doi":"10.1016/j.stress.2025.101004","DOIUrl":"10.1016/j.stress.2025.101004","url":null,"abstract":"<div><div>Upstream open reading frame (uORF)-based genetic engineering has emerged as an excellent strategy for improving agronomic traits of crops. Despite its significant potential, the exploration of CRISPR/Cas-based uORF engineering in many crop species remains unexplored, thereby limiting the application of this approach in genetic innovation of important crops. In this study, we focused on uORF-based genome editing to improve abiotic stresses in <em>Brassica napus</em>. The putative uORFs of <em>BnVTC</em> genes which involved in ascorbic acid (AsA) biosynthesis were selected as potential targets. The AsA contents in leaves, buds, and stems were significantly increased in <em>BnVTC2-uORF</em>-edited mutants. The <em>BnVTC2-uORF</em>-edited mutants exhibited tolerance to environmental stresses, such as low temperature, salinity, and drought. No obvious penalty on yield traits were observed between the <em>BnVTC2-uORF</em>-edited lines and WT. <em>VTC2</em>-uORF sequence was highly conserved across the genus Brassica, coupled with the absence of frameshift mutations in the natural germplasm, which suggested that uORF-targeted gene editing alone could be an effective approach for improving abiotic stresses. This research paves the way for the strategic deployment of CRISPR-based uORF engineering to improve the nutritional profile and abiotic stress resistance of oilseed rape.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101004"},"PeriodicalIF":6.8,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144912715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in genomics-assisted breeding strategies for enhancing nutrient uptake and use efficiency in cereals: A pathway toward sustainable agriculture","authors":"Gurjeet Singh ,&nbsp;Om Prakash Raigar ,&nbsp;Simardeep Kaur ,&nbsp;Ruchi Bishnoi ,&nbsp;Kinjal Mondal ,&nbsp;Kibrom B. Abreha ,&nbsp;Amrit Kumar Nayak ,&nbsp;Tabinda Athar ,&nbsp;Vinay Sharma ,&nbsp;Danishta Aziz ,&nbsp;Santosh Gudi ,&nbsp;Pawan Saini ,&nbsp;Amit Kumar ,&nbsp;Rakesh Bhardwaj ,&nbsp;Amritbir Riar","doi":"10.1016/j.stress.2025.101002","DOIUrl":"10.1016/j.stress.2025.101002","url":null,"abstract":"<div><div>Staple cereals such as rice, wheat, and maize are key to food security by providing the bulk of calories consumed worldwide. However, cereal productivity is often limited by inefficient uptake and utilization of essential nutrients, including N, P, Fe, and Zn especially under stress conditions. Enhancing nutrient uptake efficiency (NutrUE) in staple crops is crucial to reducing fertilizer inputs, supporting sustainable agriculture, and securing food and nutrition for future generations. The present review discusses recent advancements in genomics-assisted breeding (GAB) aimed at improving nutrient uptake (NU) in major cereals. This review delineates the biochemical and molecular underpinnings of NU, emphasizing how genomics tools such as QTL mapping, GWAS, GS, and CRISPR/Cas9 enable the dissection and targeted improvement of multifactorial NU and NutrUE-related traits. Additionally, it also explored the high-throughput phenotyping (HTP) and genotyping (HTG) platforms, including imaging techniques like MRI, X-Ray CT, and UAV-based RGB/Multispectral imaging, aligned with next-generation sequencing which enable precise and rapid characterization for NU and NutrUE-related traits. Furthermore, the review addresses how multi-omics approaches (genomics, transcriptomics, proteomics, and metabolomics) contribute to the identification of candidate genes and regulatory pathways associated with NU. Ultimately, this integrated approach provides valuable strategies and insights for researchers, breeders, and policymakers working in accelerating the development of NU and NutrUE cereal cultivars, thereby supporting agricultural sustainability and global hunger prevention.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101002"},"PeriodicalIF":6.8,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144906933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}