Plant Stress最新文献

{"title":"Mitochondrial oxidative phosphorylation (mtOXPHOS) serves as a sentinel to gauge fluctuations under heat stress in Arabidopsis thaliana elucidated by comparative transcriptomics","authors":"Muhammad Riaz ,&nbsp;Erum Yasmeen ,&nbsp;Moyang Liu ,&nbsp;Hafiz Saqib Ali ,&nbsp;Mengli Lv ,&nbsp;Hu Shi ,&nbsp;Chuanhui Du ,&nbsp;Tiantian Dong ,&nbsp;Zhenxin Liu ,&nbsp;Qingwei Song ,&nbsp;QiJun Ma ,&nbsp;Kaijing Zuo","doi":"10.1016/j.stress.2024.100613","DOIUrl":"10.1016/j.stress.2024.100613","url":null,"abstract":"<div><div>Heat stress has destructive effects on crop production and quality posing a grave threat to food security worldwide. Recent studies have elucidated the complicated transcriptional regulatory networks involved in heat stress, but how the organelles of plants adapt to heat stress remains largely unknown. To analyze the molecular mechanism of the organelle's contribution to plant heat adaptation, we utilized publicly available transcriptomic datasets to identify the central module and key pathway responding to heat stress in <em>Arabidopsis thaliana</em>. The co-expression network showed that the mitochondrial electron transport chain (mETC) and ATP synthase in the pathway of mitochondrial oxidative phosphorylation (mtOXPHOS) shows the highest correlation and ranks at the top among the characterized pathways. Comparative transcriptomic analysis indicated that the genes of the mtOXPHOS pathway and ATP synthesis exhibited different expression profiles between the roots and leaves under high temperature stress. Suppressed OXPHOS and respiration due to the dysfunction of mitochondria in MRPL1 mutants exhibited thermosensitivity. Extensive genetic reprogramming through ROS, Ca⁺², and retrograde signaling pathways that mitigate stress was also observed. In addition, NAD⁺/NADH ratio indicated redox balancing in response to high temperature. We further verified that, lower mtOXPHOS also affects photosystem II under heat stress. Hence, we concluded that appropriate mitochondrial dynamics, higher oxygen consumption rate (∼15.49-fold higher than mutant at 44 °C) and the sufficient levels of ATP production in roots (∼1.78-fold higher than mutant at 44 °C) ensure plant survival under heat stress. These findings provide valuable clues about mitochondrial signaling, OXHOPS, and energy status in response to heat stress in planta.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100613"},"PeriodicalIF":6.8,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulating reactive oxygen species and ion homeostasis for combined salt and cadmium stress tolerance in Brassica campestris: The role of beneficial microbes","authors":"Mirza Hasanuzzaman ,&nbsp;Md. Mahabub Alam ,&nbsp;Farha Naz ,&nbsp;Samiha Rummana ,&nbsp;Ayesha Siddika ,&nbsp;Abida Sultana ,&nbsp;Faomida Sinthi ,&nbsp;P.V. Vara Prasad","doi":"10.1016/j.stress.2024.100605","DOIUrl":"10.1016/j.stress.2024.100605","url":null,"abstract":"<div><div>The land areas and crop species adversely impacted by salinity and heavy metals are growing rapidly. Current research indicates that plant growth-promoting microorganisms offer an environmentally friendly option for improving physiological and biochemical processes in plants growing under stress conditions. The aim of the present study was to investigate the potential mitigation of simultaneous salinity and cadmium (Cd) stress in rapeseed (<em>Brassica campestris</em> cv. BARI Sarisha-17) by the application of <em>Azospirillum</em> sp. (Az), phosphate solubilizing bacteria (PSB), potassium mobilizing bacteria (KMB), and vesicular arbuscular mycorrhiza (VAM). Seeds were treated with PSB or KMB prior to sowing, whereas Az, PSB, KMB, or VAM were added as supplements during soil preparation. At 21 days after sowing, the plants were treated with a combination of salt (100 mM NaCl) and Cd (0.25 mM CdCl₂), with several applications at 7-day intervals. The combination of salt and Cd stress decreased plant growth and biomass, relative water content, and photosynthetic pigment levels, while also increased electrolyte leakage, lipid peroxidation, and the generation of excess reactive oxygen species (ROS). Salt and Cd stress also impaired plant ion balances of sodium, potassium and nitrate, antioxidant defenses, and glyoxalase system activity. Application of Az, PSB, or KMB restored these parameters to unstressed levels by facilitating the scavenging of ROS, maintaining water status, restoring ion balances, enhancing plant antioxidant defenses, and increasing glyoxalase enzyme activity, while reducing methylglyoxal toxicity and improving photosynthetic activity. The application of KMB was the most effective; however, all microbe supplementations showed the ability to alleviate the damage caused by stress in rapeseed. These findings highlight the ability of soil microorganisms with plant growth-promoting properties to improve the physiological and biochemical functions of rapeseed under Cd and salt stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100605"},"PeriodicalIF":6.8,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002586/pdfft?md5=883c9c4e815fe451533c9b54674977c0&pid=1-s2.0-S2667064X24002586-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical Defense Arsenal, Genes/QTLs and Transcripts for Imparting Anthracnose Resistance in Common bean (Phaseolus vulgaris L.)","authors":"Safoora Shafi ,&nbsp;Mohd Tahir ,&nbsp;Vanya Bawa ,&nbsp;Farkhandah Jan ,&nbsp;Neeraj Choudhary ,&nbsp;Mohd Anwar Khan ,&nbsp;Yogita Singh ,&nbsp;Upendra Kumar ,&nbsp;Bilal Ahmad Bhat ,&nbsp;Waseem Ahmad Dar ,&nbsp;Parvaze Ahmad Sofi ,&nbsp;Mohd. Ashraf Bhat ,&nbsp;Bilal Ahmad Padder ,&nbsp;Asif Bashir Shikari ,&nbsp;Rajneesh Paliwal ,&nbsp;Rajeev Kumar Varshney ,&nbsp;Reyazul Rouf Mir","doi":"10.1016/j.stress.2024.100609","DOIUrl":"10.1016/j.stress.2024.100609","url":null,"abstract":"<div><div>Anthracnose (ANT), caused by <em>Colletotrichum lindemuthianum</em>, is the most devastating disease affecting common bean (<em>Phaseolus vulgaris</em> L.), leading to significant yield losses in the Western Himalayas. The study provides a comprehensive understanding of ANT resistance <em>via</em> trait phenotyping, biochemical profiling, genome-wide association studies (GWASs), and RNA sequencing. The assessment of bean association mapping panel in different environments revealed a diverse spectrum of resistance levels. Biochemical analysis revealed distinctive defense responses against ANT infection among different genotypes. GWAS approach identified 24 significant marker‒trait associations (MTAs) distributed across all 11 bean chromosomes. Notably, 03 MTAs (<em>BMr205, BMr269</em> and <em>BMr244</em>) present on chromosome Pv07 were validated for ANT, and the remaining MTAs were novel MTAs for ANT. Transcriptome sequencing of resistant (PBG-3) and susceptible (PBG-26) genotypes under mock and 120-hour post inoculation conditions revealed key differentially expressed genes, such as leucine-rich repeat domain-containing protein (<em>PHAVU_</em>007G087700g), NB-ARC domain-containing protein (<em>PHAVU_003G002500g</em>) and transcription factors pivotal for disease resistance. The expression patterns of four genes (<em>PHAVU_007G087700g, PHAVU_003G002500g, PHAVU_007G056100g</em> and <em>PHAVU_003G003000g</em>) were validated through quantitative reverse transcription polymerase chain reaction (qRT‒PCR). Furthermore, the integration of GWAS-identified candidate genes with transcriptomics and cross-referencing with previous studies validated overlapping regions and common candidate genes, enriching our understanding of the genetic basis of ANT resistance. Therefore, the results offer a holistic perspective on ANT resistance in common bean, providing a foundation for targeted breeding efforts. The identified potential candidate genes and associated pathways will contribute valuable insights into the development of ANT resistant common bean varieties.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100609"},"PeriodicalIF":6.8,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142422077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regional propolis extracts suppress Fusarium fujikuroi and boost rice seedling growth and response against Bakanae disease","authors":"Zeinab A. Kalboush ,&nbsp;Yasser S.A. Mazrou ,&nbsp;Sara H. Elzan ,&nbsp;Eatemad M. Zanaty ,&nbsp;Alyaa A.A. Gazzy ,&nbsp;Marwa B.M. Gomaa ,&nbsp;Abeer H. Makhlouf ,&nbsp;Yasser Nehela","doi":"10.1016/j.stress.2024.100610","DOIUrl":"10.1016/j.stress.2024.100610","url":null,"abstract":"<div><div>Bakanae, caused by Fusarium fujikuroi, is a serious seed-borne disease affecting rice production worldwide. Herein, we investigated the potential of propolis, a natural honeybee product, collected from three geographic locations in Egypt: North (Menofia), Middle (Cairo), and South (Sohag), and extracted with four different solvents (ethanol, olive oil, hexane, and water) as a sustainable eco-friendly alternative to control the Bakanae disease in rice. In vitro experiments showed that propolis extracts exhibited antifungal activity against F. fujikuroi and significantly suppressed its mycelial growth and sporulation. The antifungal activity of propolis extracts was associated with its chemical composition which varied depending on geographical origin, and the extraction solvents. Moreover, SEM-based analysis revealed that ethanolic extract of northern propolis prominently altered the microconidia morphology of F fujikuroi, which shrunken and seemed to lose their viability. These findings were further confirmed in vivo under greenhouse conditions. Soaking F fujikuroi-inoculated rice seeds in 1000 ppm of propolis extract significantly reduced the bakanae disease incidence, and disease severity index compared to mock-treated controls. Although infection with F fujikuroi induced the accumulation of H₂O₂, the application of propolis extracts alleviated oxidative stress and significantly reduced the H₂O₂ levels within infected rice seedlings. Moreover, propolis extracts enhanced the profile of enzymatic antioxidants (guaiacol-dependent peroxidase [POX] and polyphenol oxidase [PPO]) in F. fujikuroi-infected rice seedlings. Finally, Propolis extracts-treated rice seedlings exhibited higher transcript levels of three PTI-marker genes including nonexpressor of pathogenesis-related genes 1 (OsNPR1), WRKY transcription factor 21(OsWRKY21), and phenylalanine ammonia-lyase (OsPAL1), which are associated with systemic acquired resistance (SAR) and crucial for the plant's defense response against pathogens. Collectively, these findings suggest that propolis might be a promising sustainable, eco-friendly alternative to control bakanae disease and other fungal seed-borne phytopathogens due to its antifungal properties and ability to induce a complex multilayered defense system within infected plants.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100610"},"PeriodicalIF":6.8,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X2400263X/pdfft?md5=fb28b8b594498426df92d612c53560b5&pid=1-s2.0-S2667064X2400263X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methyl jasmonate enhances rice tolerance to alkaline stress via the auxin pathway","authors":"Chun-Lan Chen ,&nbsp;Di Wu ,&nbsp;Qian-Kun Li ,&nbsp;Xiao-hu Liu ,&nbsp;Xu-Guang Niu ,&nbsp;Guo-Xian Zhang ,&nbsp;Yong-Yong Zhang ,&nbsp;Hui Zhang ,&nbsp;Chang-Jie Jiang","doi":"10.1016/j.stress.2024.100612","DOIUrl":"10.1016/j.stress.2024.100612","url":null,"abstract":"<div><div>Soil alkalization is a major challenge for global crop production. This study reveals a novel defense mechanism in rice seedlings against alkaline stress, involving methyl jasmonate (MeJA) and auxin pathways. Under alkaline stress (15 mM Na₂CO₃), rice seedlings exhibited elevated levels of endogenous MeJA and upregulation of JA-responsive genes. Pre-treatment with MeJA (50 µM) significantly improved seedling survival, growth, and mitigated root damage under alkaline stress. This treatment also upregulated genes associated with cell death suppression (<em>OsBI1</em>) and stress tolerance (<em>OsJRL, OsNAC</em>). Notably, MeJA pre-treatment increased auxin (indole-3-acetic acid, IAA) levels in roots, and upregulated genes involved in IAA synthesis (<em>OASA1, OASA2</em>) and auxin signaling (<em>Aux/IAA, ARFs</em>). Blocking auxin transport with N-1-naphthylphthalamic acid intensified root damage under alkaline stress and diminished the protective effect of MeJA. These results highlight the crucial role of MeJA-induced activation of auxin pathway in enhancing rice tolerance to alkaline stress, and provide valuable insights for developing strategies to improve crop resilience in alkaline soils.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100612"},"PeriodicalIF":6.8,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002653/pdfft?md5=7cafd528d85383efc51b74cb1a9eea8b&pid=1-s2.0-S2667064X24002653-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudomonas consortium improves soil health and alleviates cadmium (Cd) toxicity in Brassica juncea L. via biochemical and in silico approaches","authors":"Tamanna Bhardwaj ,&nbsp;Ruby Singh ,&nbsp;Harpreet Singh ,&nbsp;Rajendra Bhanwaria ,&nbsp;Sumit G. Gandhi ,&nbsp;Renu Bhardwaj ,&nbsp;Ajaz Ahmad ,&nbsp;Parvaiz Ahmad","doi":"10.1016/j.stress.2024.100611","DOIUrl":"10.1016/j.stress.2024.100611","url":null,"abstract":"<div><div>The exponential rise in Cd in the environment has raised concerns for its adequate remediation worldwide. Its non-biodegradable nature and highly migratory feature make it more toxic. Plant growth-promoting rhizobacteria (PGPR) have shown great potential in the remediation of Cd-polluted agricultural lands. PGPR is comparatively efficient, convenient and economical. PGPR application enhances plant growth and development by conferring direct and indirect benefits. This study aimed to evaluate bacterial strains, <em>Pseudomonas putida (Pp)</em> and <em>Pseudomonas fluorescens (Pf),</em> for their PGPR traits, soil physiochemical analyses, and physiological and anatomical traits of <em>B. juncea</em> under Cd stress. Results showed that both microbial strains shared positive interaction and had minimum inhibitory concentration (MIC) ranging from 0.8mM and 0.6mM values for Pp and Pf respectively. <em>P. fluorescens</em> displayed better anti-phytopathogenic activity against pathogenic fungal strains (<em>Alterneria brassicae, Alterneria brassicola, Verticillium longisporum, Fusarium oxysporum</em>) than <em>P. putida</em>. They also synthesised plant growth regulators (PGRs) such as IAA (0.146,0.156μg/ml) and GA (2.062, 2.074 μg/ml). The co-inoculation of strains improved soil organic C, P, N, and K by 283.01 %, 100.42 %, 8.89 % and 40.38 %. Also, the interactive effect of Pp and Pf recovered dry matter content (DMC) by 18.13 % in comparison to Cd-stressed plants. Moreover, the strains reduced Cd-induced H₂O₂ production by DAB (340.38’-3’ diaminobenzidine) staining and hence restored membrane integrity. Also, in Cd-treated <em>B. juncea</em> plants, the anatomical characteristics were negatively affected. However, inoculated strains induce maximum recovery as indicated by well-developed vascular elements. Genes associated with PGPR traits were mined from the NCBI database. The information compiled thereafter indicated that the genes, <em>galU, CadR,</em> and <em>pgl</em> were responsible for biofilm formation, Cd resistance and lactone synthesis. In conclusion, we reported a promising consortium having PGPR traits, that improve soil and <em>B. juncea</em> health under Cd toxicities. Hence, the use of such bioinoculants can be a safer substitute for chemical fertilizers.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100611"},"PeriodicalIF":6.8,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of SIMK in menadione-treated alfalfa enhances antioxidant machinery and leads to oxidative stress resistance","authors":"Jiří Sojka ,&nbsp;Tomáš Takáč ,&nbsp;Kateřina Hlaváčková ,&nbsp;Pavol Melicher ,&nbsp;Miroslav Ovečka ,&nbsp;Tibor Pechan ,&nbsp;Jozef Šamaj","doi":"10.1016/j.stress.2024.100608","DOIUrl":"10.1016/j.stress.2024.100608","url":null,"abstract":"<div><div>Mitogen-activated protein kinases (MAPKs) transduce stress and developmental signals related to the production of reactive oxygen species (ROS). Alfalfa (<em>Medicago sativa</em> L.) is a valuable forage and human nutrition crop, however, the involvement of MAPKs in plant resistance to oxidative stress is poorly understood in this species. Therefore, we elucidated the role of STRESS-INDUCED MAPK (SIMK) in alfalfa response to menadione, a compound inducing ROS generation, exploiting transgenic alfalfa lines with contrasting SIMK abundance. SIMK was activated by short-term menadione treatment and relocated from the nucleus to the cytoplasm. Proteomic analysis revealed that menadione caused changes in the abundance of proteins involved in metabolism, oxidative stress, biotic stress response, detoxification of carbonyl species, glutathione homeostasis, chloroplast protein turnover, photosynthesis, and membrane trafficking. Genetic manipulations of SIMK altered the abundance of proteins involved in mitochondrial and chloroplast protein import and processing, as well as GLUTATHIONE S-TRANSFERASES (GSTs). Increased GST abundance and activity in roots, and modifications in mitochondrial and chloroplast protein turnover might be responsible for the elevated oxidative stress resistance of alfalfa line overexpressing SIMK. This was supported by the reduced ROS levels in this line. These results reveal a complex nature of plant stress response and suggest a new role of SIMK in the alfalfa resistance to menadione-induced oxidative stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100608"},"PeriodicalIF":6.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early and late responses to Fusarium Head blight in durum wheat: Focus on phenylpropanoid biosynthetic pathway","authors":"Linda Felici ,&nbsp;Federica Castellani ,&nbsp;Sara Francesconi ,&nbsp;Matteo Vitali ,&nbsp;Francesco Sestili ,&nbsp;Giorgio Mariano Balestra","doi":"10.1016/j.stress.2024.100603","DOIUrl":"10.1016/j.stress.2024.100603","url":null,"abstract":"<div><p>Durum wheat is among the cereal crops most susceptible to Fusarium Head Blight (FHB), a fungal disease that can lead to significant yield losses. Despite this, only limited research efforts have been directed towards understanding FHB resistance in durum wheat. Wheat grains naturally contain phenolic compounds, and anthocyanins are particularly present in the so-called pigmented wheat genotypes, such as purple pericarp ones. In this study the effects of the biotic stress caused by <em>Fusarium graminearum</em> infection on phenylpropanoid biosynthetic pathway in durum wheat spikes were explored, considering three genotypes with different susceptibility (including a purple pericarp genotype), and two time points (an early stage time point: 2 days post infection, and a late stage time point: 21 days post infection). At early infection stage, the <em>F. graminearum</em> infection triggered upregulation of all the considered genes involved in the phenylpropanoid pathway in the resistant genotype, while, in the purple pericarp genotype, the infection caused an increase in quercetin accumulation in the soluble fraction of spike extract. At late infection stage, the infection caused (in all the genotypes) a degradation of secondary cell wall and the release of the hydroxycinnamic acids esterified with arabinoxylans (ferulic acid and <em>p-</em>coumaric acid) and lignin-derived monomers (vanillic acid). Furthermore, chalcone synthase gene (<em>CHS</em>) and the transcription factor <em>Ppm1</em> (Purple pericarp MYB 1) were boosted in the pigmented genotype due to infection at late infection stage. These findings contribute to the understanding of host-pathogen interactions for future breeding programs focused on improving FHB resistance in durum wheat varieties, with a particular focus on pigmented genotypes.</p></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100603"},"PeriodicalIF":6.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002562/pdfft?md5=e3ece17f0609514e13a2a7f1b5120c9d&pid=1-s2.0-S2667064X24002562-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142272552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analysis of apple CNGC family allows the identification of MdCNGC15A negatively regulating apple salt tolerance","authors":"Lina Qiu ,&nbsp;Chuang Mei ,&nbsp;Zhiping Qi ,&nbsp;Jiaxin Yang ,&nbsp;Na Li ,&nbsp;Ming Li ,&nbsp;Yunxia Sun ,&nbsp;Jie Yang ,&nbsp;Fengwang Ma ,&nbsp;Ke Mao","doi":"10.1016/j.stress.2024.100606","DOIUrl":"10.1016/j.stress.2024.100606","url":null,"abstract":"<div><p>Calcium (Ca²⁺) is essential for signal conduction and plant growth. Cyclic nucleotide-gated channels (CNGCs) are Ca²⁺ transporters that regulate Ca²⁺ signalling and homeostasis by modulating its transmembrane transport, thereby influencing plant development as well as the biotic and abiotic stress responses. Although identified in numerous plant species, the CNGC family has not been characterized in apple until now. Here, 20 <em>MdCNGC</em>s were identified from the apple genome and were randomly distributed on 13 chromosomes. Phylogenetic analysis classified these <em>MdCNGCs</em> into five groups (I, Ⅱ, Ⅲ, Ⅳ-a, and Ⅳ-b), with five pairs of segmental duplicated genes being detected via collinearity analysis. Sequence alignment and analyses of gene structures, conserved motifs, and 3D structures indicated high structural conservation, particularly within groups. Yeast two-hybrid (Y2H) assays demonstrated interactions between most MdCNGCs and the Ca²⁺ receptor MdCaM7.1, except for MdCNGC1B and MdCNGC15A. Promoter analysis and expression profiling revealed significant responses to abiotic stress, particularly salt stress, in some <em>MdCNGCs</em>. Silencing <em>MdCNGC15A</em> significantly enhanced apple plants salt tolerance, while its overexpression in apple calli significantly decreased tolerance, as shown by transgenic analysis. Collectively, our results demonstrate the crucial role of <em>MdCNGCs</em> in abiotic stress responses and provide valuable insights for future functional and regulatory studies in apples.</p></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100606"},"PeriodicalIF":6.8,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002598/pdfft?md5=20c21f79ff04898b54b63bae388b952a&pid=1-s2.0-S2667064X24002598-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142272533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsCSLD4 confers salt–alkali tolerance by regulating gene expressions in photosynthesis and carbohydrate biosynthesis pathways, cell wall hemicellulose accumulation and physio-biochemical adaptability in rice","authors":"Zhijian Liu ,&nbsp;Cuili Wu ,&nbsp;Wenjie Li ,&nbsp;Li Hu ,&nbsp;Xingyue Fu ,&nbsp;Binhua Hu ,&nbsp;Yongxiang Liao ,&nbsp;Zufen Xiang ,&nbsp;Haibo Jiang ,&nbsp;Weizao Huang ,&nbsp;Xiaocheng Yang ,&nbsp;Anping Du ,&nbsp;Zhigang Pu ,&nbsp;Shengbin Tu ,&nbsp;Xinrong Ma ,&nbsp;Hui Li","doi":"10.1016/j.stress.2024.100604","DOIUrl":"10.1016/j.stress.2024.100604","url":null,"abstract":"<div><div>In rice, cell-wall matrix polysaccharides contribute to salt–alkali tolerance. However, the mechanism by which cell-wall matrix polysaccharides and their biosynthetic genes regulate salt–alkali tolerance in rice remains unclear. To address this question, we report on the regulatory mechanism of salt–alkali tolerance of the 1,4-β-d-xylan synthase gene <em>OsCSLD4</em> in the hemicellulose biosynthesis pathway. Mutant of <em>OsCSLD4, nd1</em> and its wild-type were analyzed using comprehensive techniques and methods, including phenotyping, gene expression, comparative transcriptomic analysis, qPCR validation, and determination of physio-biochemical indices. We found that the salt–alkali tolerance of <em>nd1</em> was lower than that of the wild type, and the expression of the <em>OsCSLD4</em> gene was induced under salt–alkali stress. Comparative transcriptomic analysis revealed that the expression levels of genes involved in photosynthesis, carbohydrate, and cell wall matrix polysaccharide biosynthesis pathways in <em>nd1</em> seedlings were downregulated compared to those in the wild type under salt–alkali stress. Accordingly, physio-biochemical analysis demonstrated that <em>nd1</em> seedlings had reduced levels of chlorophyll, total soluble sugar, starch, and hemicellulose, coupled with a significant increase in malondialdehyde content under salt–alkali stress. In essence, the <em>OsCSLD4</em> gene confers salt–alkali tolerance to rice by regulating the hemicellulose content to strengthen cell wall integrity and enhance intracellular physio-biochemical salt–alkali tolerance at the cellular level, thereby maintaining photosynthetic capacity and growth at the plant level. This study revealed that <em>OsCSLD4</em> has potential value in molecular breeding for the development of salt-alkali-tolerant rice varieties.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100604"},"PeriodicalIF":6.8,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002574/pdfft?md5=74fe197c20b2fdde31ca2b2e939235f7&pid=1-s2.0-S2667064X24002574-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}