Plant Stress最新文献

{"title":"Silicon-driven approaches to salinity stress tolerance: Mechanisms, uptake dynamics, and microbial transformations","authors":"Gajendiran Manimaran ,&nbsp;Selvi Duraisamy ,&nbsp;Thiyageshwari Subramanium ,&nbsp;Anandham Rangasamy ,&nbsp;Senthil Alagarsamy ,&nbsp;Prabhaharan James ,&nbsp;Selvababu Selvamani ,&nbsp;Deepana Perumal ,&nbsp;Mageshen Veerappan ,&nbsp;Yuvasri Errakutty Arunan ,&nbsp;Jegan Periakaruppan","doi":"10.1016/j.stress.2025.100825","DOIUrl":"10.1016/j.stress.2025.100825","url":null,"abstract":"<div><div>Salinity stress poses a critical threat to global crop productivity, driven by factors such as saline irrigation, low precipitation, native rock weathering, high surface evaporation, and excessive fertilizer application. This abiotic stress induces oxidative damage, osmotic imbalance, and ionic toxicity, severely affecting plant growth and leading to crop failure. Silicon (Si) has emerged as a versatile element capable of mitigating various biotic and abiotic stresses, including salinity. This review offers a comprehensive analysis of Si's multifaceted role in alleviating salinity stress, elucidating its molecular, physiological, and biochemical mechanisms in plants. It explores Si uptake, transport, and accumulation in plant tissues, emphasizing its contributions to maintaining ionic balance, enhancing water uptake, and reinforcing cell structural integrity under saline conditions. Additionally, this review addresses Si transformations in saline soils and the factors influencing its bioavailability. A significant focus is placed on silicon-solubilizing microorganisms (SSMs), which enhance Si bioavailability through mechanisms such as organic acid production, ligand exchange, mineral dissolution, and biofilm formation. By improving nutrient cycling and mitigating salinity-induced stress, SSMs offer a sustainable alternative to synthetic silicon fertilizers, promoting resilient crop production in salt-affected soils.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100825"},"PeriodicalIF":6.8,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Auxin-induced WRKY23 activates PECTIN LYASE-LIKE1 and PECTIN LYASE-LIKE3 for apoplastic iron reutilization in Arabidopsis roots","authors":"Yuzhen Zhang ,&nbsp;Lihong Hong ,&nbsp;Xiaoya Feng ,&nbsp;Ru'nan Huang ,&nbsp;Li Huang ,&nbsp;Yuhuan Wu ,&nbsp;Weiwei Chen","doi":"10.1016/j.stress.2025.100830","DOIUrl":"10.1016/j.stress.2025.100830","url":null,"abstract":"<div><div>The retention and reutilization of apoplastic iron (Fe) are essential for Fe homeostasis in plants, yet the underlying molecular mechanisms remain largely unexplored. Here, we characterized the role of WRKY23, a nucleus-localized transcription factor, in regulating apoplastic Fe retention and reutilization in response to Fe deficiency in <em>Arabidopsis thaliana</em>. Under Fe deficiency, the induction of <em>WRKY23</em> expression is modulated by local auxin signaling. Once activated, WRKY23 then influenced Fe homeostasis by regulating pectin metabolism and Fe-binding capacity in the cell wall. Notably, WRKY23 could directly bind to W-box motifs in the promoters of target genes, including <em>PECTIN LYASE-LIKE1</em> (<em>PLL1</em>) and <em>PLL3</em>, activating their transcription. Collectively, our findings support a model in which WRKY23 functions as part of a transcriptional cascade, whereby auxin signaling promotes the role of WRKY23 in regulating pectin degradation and enhancing Fe retention and reutilization in the apoplast, thereby negatively modulating Fe deficiency responses in roots. This research deepens our understanding of plant responses to nutritional stress and may inform strategies for improving crop resilience.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100830"},"PeriodicalIF":6.8,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elicitor-induced stilbene production and lignin reduction in peanut hairy roots: Insights from transcriptomic and metabolomic analysis","authors":"Apinun Limmongkon ,&nbsp;Phadtraphorn Chayjarung ,&nbsp;Chanyanut Pankaew ,&nbsp;Sompop Pinit ,&nbsp;Nitra Nuengchamnong ,&nbsp;Chonnikan Tothong","doi":"10.1016/j.stress.2025.100823","DOIUrl":"10.1016/j.stress.2025.100823","url":null,"abstract":"<div><div>Secondary metabolites are crucial for plant defense. This study investigates the time-dependent transcriptomic and metabolomic responses of peanut hairy root cultures to a combined elicitor treatment of chitosan (CHT), methyl jasmonate (MeJA), and cyclodextrin (CD). Differentially accumulated metabolites (DAMs), particularly stilbenes and phenolic/flavonoid compounds, increased significantly compared to the baseline group. Phenolic, flavonoid, and stilbene levels rose, while lignin content decreased over time. Transcriptomic analysis revealed upregulation of key genes in the phenylpropanoid pathway, including <em>PAL, C4H,</em> and <em>4CL</em>, which correlated with elevated levels of hydroxybenzoic acid and related compounds. Genes involved in stilbene biosynthesis, such as <em>STS, ROMT, R4DT-1</em>, and <em>R3’DT-4</em>, as well as flavonoid biosynthesis genes, including <em>CHS, CHI, F3H, CHR, FLS</em>, and <em>UGT72E</em>, were also upregulated, corresponding to the accumulation of their respective metabolites. In contrast, lignin biosynthesis genes, such as <em>HCT, CSE, CCoAOMT, CCR, CAD,</em> and <em>POD</em>, were downregulated, while lignin-degrading genes were upregulated. This was further supported by tissue staining results and the reduction of lignin content during the elicitation period. The shift from lignin synthesis to degradation underscores a redirection of metabolic flux toward the production of defense-related secondary metabolites, particularly stilbenes, phenolics, and flavonoids.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100823"},"PeriodicalIF":6.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of ASR (ABA, Stress, and Ripening) genes in responses to phosphate starvation in rice roots","authors":"Nicolle Louise Ferreira Barros ,&nbsp;Breno Xavier Gonçalves ,&nbsp;Thomaz Stumpf Trenz ,&nbsp;Paloma Koprovski Menguer ,&nbsp;Lucas Roani Ponte ,&nbsp;Cristiane P.G. Calixto ,&nbsp;Felipe Klein Ricachenevsky ,&nbsp;Marcia Margis-Pinheiro","doi":"10.1016/j.stress.2025.100824","DOIUrl":"10.1016/j.stress.2025.100824","url":null,"abstract":"<div><div>Phosphorus (P) is a crucial macronutrient for plant growth and development, absorbed by plant roots as inorganic phosphate, which is frequently limited in soil. Plants use only 30 % of the total phosphate fertilizers applied to increase yield. Compared to other nutrients, the understanding of the molecular mechanisms involved in phosphate homeostasis in crops, particularly in the early transcriptional responses to change the root system architecture remain underexplored. Addressing these knowledge gaps requires studies that offer insights into the role of transcription factors in response to endogenous and exogenous signals associated with the nutritional status of crops. ASR (ABA, Stress and Ripening) proteins function as molecular chaperones, transcription factors, and homeostasis sensors. They also regulate the development and response to stress in plants. Our results show that <em>ASR</em> genes play an important role in phosphate homeostasis in rice (<em>Oryza sativa</em> L.) roots. Silencing of <em>OsASR</em> genes (OsASR-RNAi plants) delays development of adventitious and lateral roots, and alters the expression of genes associated with root development and the response to phosphate starvation. These findings suggest that <em>OsASR</em> play a role in regulating root system architecture, nutrient perception and signal transduction in rice plants.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100824"},"PeriodicalIF":6.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interaction between autophagy-related gene FtATG8a and FtE2FB is involved in the drought resistance of Tartary buckwheat","authors":"Shuang Wang ,&nbsp;XueYan Fang ,&nbsp;Chang Zhang,&nbsp;Zhi Shan,&nbsp;XinYu Zhang,&nbsp;Yi Wu,&nbsp;ShuWen Zhang,&nbsp;Tao Wang,&nbsp;Qi Wu","doi":"10.1016/j.stress.2025.100821","DOIUrl":"10.1016/j.stress.2025.100821","url":null,"abstract":"<div><div>Drought significantly limits worldwide crop yields, with autophagy acting as an essential regulatory component in plant adaptation to stress. In Tartary buckwheat, while there has been evidence of autophagosome accumulation and increased levels of <em>FtATG8a</em> due to drought, the molecular pathways governing <em>FtATG8a</em> have not yet been clarified. Our research shows that overexpressing <em>FtATG8a</em> markedly improves drought tolerance in genetically modified plants by synchronizing the activation of autophagy, boosting antioxidant defenses (such as SOD, POD, and CAT), and promoting proline biosynthesis. Through yeast two-hybrid screening, we identified FtE2FB as a nuclear-localized partner that interacts with FtATG8a, with their interaction facilitated by a conserved ATG8-interacting motif (EK<u>F</u>ED<u>I</u>) found in FtE2FB, which was validated using various complementary assays. Importantly, <em>FtE2FB</em> expression demonstrated dual induction patterns in response to drought stress and the overexpression of <em>FtATG8a</em>, indicating a feedback regulatory mechanism. Functional experiments showed that the solitary overexpression of <em>FtE2FB</em> boosts drought resistance via the modulation of the antioxidant system and increased proline levels in <em>Arabidopsis</em>. Additionally, the combined expression of <em>FtATG8a</em> and <em>FtE2FB</em> led to a more substantial rise in both antioxidant enzyme activity and proline content when compared to the overexpression of either <em>FtE2FB</em> or <em>FtATG8a</em> on its own under 20 % PEG6000 treatment. This study clarifies an autophagy-related regulatory network that underpins drought adaptation in Tartary buckwheat, offering valuable mechanistic insights into the coordination of stress responses and highlighting potential molecular targets for enhancing crop improvement strategies.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100821"},"PeriodicalIF":6.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Over-expression analysis of TaDWF4˗4B in mutant wheat lines identifies a candidate regulator of heat stress tolerance","authors":"Liaqat Shah ,&nbsp;Muhammad Saeed ,&nbsp;Muhammad Ibrahim ,&nbsp;Waqas Ahmad ,&nbsp;Abdul Rahman Umar ,&nbsp;Ayan Sohail ,&nbsp;Hongqi Si","doi":"10.1016/j.stress.2025.100822","DOIUrl":"10.1016/j.stress.2025.100822","url":null,"abstract":"<div><div>Wheat production faces great threat due to rising environmental temperatures resulting from climate change. Here we analyzed a bulk of wheat exotic, elite, synthetic and local wheat lines to evaluate the impact of heat stress on the physiological and biochemical parameters of different genotypes. Mutant susceptible genotypes were generated by treating with Ethyl Methane Sulfonate (EMS) and its performance was evaluated under normal and heat stress conditions. Based on the heat stress index and yield measurement, the lines were grouped into four classes, i.e. tolerant (T), Susceptible (S), moderately tolerant (MT), and moderately susceptible (MS). The results showed significant impact of heat stress on all parameters of the tested gene pool; however, the affect was less intense on the tolerant lines compared to the other classes. To validate heat stress tolerance, we conducted RNA sequencing analysis and identified eight genes associated with heat tolerance. Among them, <em>TaDWF4˗4B</em> showed highest expression under heat stress condition and selected for further functional analysis. Overexpression of <em>TaDWF4˗4B</em> in the wheat line ESWYT-4 enhanced heat tolerance. Treatment with 2 μM brassinosteroids (BR) decreased seed germination in the transgenic lines, suggesting that <em>TaDWF4˗4B</em> enhances BR response. Endogenous BR contents increased in overexpression lines, along with increasing the expression of several BR biosynthetic pathway genes in overexpression line under heat stress condition. Moreover, the overexpression of <em>TaDWF4˗4B</em> improved reactive oxygen species (ROS) scavenging by increasing the activities of <em>TaCAT3, TaSOD1</em>, and <em>TaGPx1</em>-<em>D</em> under heat stress condition. These findings indicate that <em>TaDWF4˗4B</em> plays an important role in regulating BR biosynthesis, increasing BR response, and ROS scavenging under heat stress condition. These results present mechanistic insights into the role of <em>TaDWF4˗4B</em> in plant responses under heat stress condition.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100822"},"PeriodicalIF":6.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidation of fruit cracking mechanism in bael [Aegle marmelos (L.) Correa.] using physico-biochemical and de novo transcriptomic approaches","authors":"Vasanth Vinayak Vara Prasad ,&nbsp;V.B. Patel ,&nbsp;M.K. Dhakar ,&nbsp;Bikash Das ,&nbsp;Sujit Kumar Bishi ,&nbsp;V.P. Bhadana ,&nbsp;G.P. Mishra ,&nbsp;Vishal Mhetre ,&nbsp;S.K. Singh ,&nbsp;Vishal Nath ,&nbsp;Ram Asrey ,&nbsp;Devendra Pandey","doi":"10.1016/j.stress.2025.100819","DOIUrl":"10.1016/j.stress.2025.100819","url":null,"abstract":"<div><div>Fruit cracking in Bael [<em>Aegle marmelos</em> (L) Correa.] is a major physiological disorder which is influenced by factors like water stress, nutrient deficiency, and environmental conditions. This study aimed to identify key biochemical constituents, genes, and pathways affecting fruit cracking using physical, biochemical, and transcriptomic analyses. Bael genotypes were categorized into three groups based on cracking incidence as tolerant (0 % cracking), moderately tolerant (&gt;0–30 % cracking), and susceptible (&gt;30 % cracking). Three genotypes from each category were selected for further analysis. Biochemical profiling revealed that total flavonoids, antioxidants, vanillic acid and soluble carbohydrates were predominant in the cracking-susceptible genotypes, while calcium and boron levels were significantly lower in these genotypes. Transcriptomic analysis using susceptible (Pant Aparna) and tolerant genotypes (ICAR-RCER 8–5) identified differentially expressed genes (DEGs) associated with cell wall and polysaccharide metabolism, phenolics and flavonoid biosynthesis, plant hormone biosynthesis and signaling, nutrient transport. Interestingly, aquaporin-encoding genes were found significantly upregulated in the cracking stage, while genes involved in MAPK signaling showed higher expression in the susceptible genotype. These transcriptomic changes were corroborated by biochemical findings, reinforcing their role in bael fruit cracking susceptibility. The insights gained from this study provide a foundation for developing cracking-tolerant bael cultivars and formulating management strategies to mitigate fruit cracking in bael.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100819"},"PeriodicalIF":6.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and ultrastructural insights into zinc-induced hormesis in wheat seedlings: Glutathione-mediated antioxidant defense in zinc toxicity regulation","authors":"Qiujuan Jiao ,&nbsp;Lina Fan ,&nbsp;Huihong Zhang ,&nbsp;Jingjing Zhang ,&nbsp;Ying Jiang ,&nbsp;Jin Yang ,&nbsp;Gezi Li ,&nbsp;Shah Fahad ,&nbsp;Evgenios Agathokleous ,&nbsp;Yinglong Chen ,&nbsp;Ajaz Ahmad ,&nbsp;Parvaiz Ahmad ,&nbsp;Shiliang Liu ,&nbsp;Haitao Liu","doi":"10.1016/j.stress.2025.100820","DOIUrl":"10.1016/j.stress.2025.100820","url":null,"abstract":"<div><div>Zinc (Zn), an essential nutrient element, exhibits hormesis in plants-beneficial at low doses but toxic at high concentrations. To understand the molecular mechanisms underlying this hormetic response with low-dose stimulation and high-dose inhibition in wheat, we conducted transcriptomic analysis under different Zn treatments. Low Zn concentration (50 μM) promoted plant growth by maintaining chlorophyll content, enhancing MAPK signaling, phytohormone signaling, glutathione metabolism, nitrogen metabolism, and cell wall polysaccharide biosynthesis. High Zn concentration (500 μM) induced ultrastructural damage and suppressed photosynthesis, chlorophyll metabolism, and secondary metabolisms, while upregulating glutathione metabolism. Molecular docking revealed that hydrogen bonds between Zn and antioxidant enzymes facilitated reactive oxygen species scavenging. Notably, exogenous glutathione (GSH) application enhanced wheat tolerance to Zn stress by strengthening the antioxidant defense system and improving photosynthetic capacity. Our findings elucidate the underlying mechanisms of Zn hormesis in wheat and demonstrate the application potential of glutathione in mitigating Zn toxicity, providing strategies for managing Zn-contaminated soils.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100820"},"PeriodicalIF":6.8,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium increases the capacity of antioxidative defense and their accompanying metal cofactors in maize under sulfate salinity","authors":"Kashif Saeed,&nbsp;Md Arif Hussain,&nbsp;Muna Ali Abdalla,&nbsp;Karl Hermann Mühling","doi":"10.1016/j.stress.2025.100816","DOIUrl":"10.1016/j.stress.2025.100816","url":null,"abstract":"<div><div>The most prevalent salts in saline soils are sodium salts like NaCl and Na₂SO₄. Various salinity forms are the most harmful abiotic stress, which can affect plants' growth, uptake of nutrients, and antioxidant machinery. Selenium (Se) confers resistance to salinity by stimulating the ROS detoxification, which are over-accumulated due to stress. Three doses of Se (0, 10, and 50 µM) were applied to hydroponically grown maize under both Cl⁻ and SO₄²⁻-salinity at an equimolar Na⁺ concentration of 100 mM to evaluate the impact of Se on important enzyme activities and nutrient uptake. Higher levels of MDA content under Cl⁻-salinity were associated with higher levels of oxidative stress in plants, demonstrating that Cl⁻-salinity has more harmful effects than SO₄²⁻-salinity. Se application considerably increased the Se concentration in plant tissues, but SO₄²⁻-salinity caused less absorption due to competition. Compared to Se-free shoots, moderate Se dramatically increased the levels of metals, including Zn, Mn, Fe, and Cu, essential for enzyme function under SO₄²⁻-salinity conditions. In contrast, no significant effects were observed under Cl⁻-salinity conditions. Se at a moderate level promoted antioxidant capacity by significantly inducing the enzymatic activities (APX, CAT, GR, SOD, and GPX) in similar patterns under the SO₄²⁻-salinity condition. Whereas, compared to Se0, moderate Se level elevated SOD, CAT, and GPX activities under Cl⁻-salinity, indicating Se's protective and antioxidative role under salinity. The results suggest that even with low uptake due to high SO₄²⁻ availability, moderate Se supply increases the absorption of metal cofactors (micronutrients). This, in turn, enhances antioxidative defenses, enabling maize crops to better tolerate salt stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100816"},"PeriodicalIF":6.8,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143688030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of microbial biofertilizer on proteomic profiling, antioxidant enzyme and andrographolide content in Andrographis paniculata Burm.f Nee. under drought stress","authors":"Butsakorn Yodphet ,&nbsp;Nuntavun Riddech ,&nbsp;Wanwipa Kaewpradit ,&nbsp;Sittiruk Roytrakul ,&nbsp;Sophon Boonlue ,&nbsp;Nisachon Jangpromma","doi":"10.1016/j.stress.2025.100817","DOIUrl":"10.1016/j.stress.2025.100817","url":null,"abstract":"<div><div><em>Andrographis paniculata</em> is a medicinal plant susceptible to drought stress. This study, employing a completely randomized design, examines the impact of drought stress on the growth and andrographolide content of <em>A. paniculata.</em> Microbial biofertilizers were investigated for their ability to mitigate drought stress and enhance resilience in <em>Andrographis paniculata</em>. Their effectiveness was evidenced by plants supplemented with microbial biofertilizers under drought stress (T4) maintaining significantly higher leaf relative water content (RWC) at 78.75 %, compared to unfertilized drought-stressed plants (T2) with only 22.37 % RWC. Microbial biofertilized <em>A. paniculata</em> also exhibited reduced activity of superoxide dismutase (SOD) and peroxidase (POX), indicating mitigated oxidative stress. Importantly, high-performance liquid chromatography (HPLC) analysis revealed that microbial biofertilizer significantly increased andrographolide content, a key bioactive compound, even under drought stress. Proteomic analysis identified key stress responses and photosynthetic proteins upregulated by microbial biofertilizers, particularly under drought. <em>A. paniculata</em> treated with microbial biofertilizers under well-watered conditions (T3) showed increased levels of proteins involved in photosynthesis and stress response (cytochrome F, ATP synthase), and drought tolerance (Kaurene synthase 1). The key photosynthetic enzyme RuBisCO displayed a 2.48-fold increase in T4, suggesting improved photosynthetic efficiency. Unique protein expressions in T4, including ribosomal proteins and UDP-glycosyltransferase, suggest enhanced drought tolerance. Furthermore, consistent upregulation of NAD(P)H-quinone oxidoreductase subunit 5 indicates improved photosynthesis and resilience under both well-watered and drought conditions. Overall, microbial biofertilizers modulated protein expression, enhancing drought tolerance of <em>A. paniculata</em> by improving stress response, photosynthetic capacity, and potentially other cellular processes.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100817"},"PeriodicalIF":6.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}