Plant Stress最新文献

{"title":"Transcriptomics and metabolomics analysis reveal the key regulator in BTH-induced fruit resistance of banana","authors":"Faiz Ur Rahman ,&nbsp;Yulin Yao ,&nbsp;Xiaoying Xie,&nbsp;Jiangping Chen,&nbsp;Ke Ma,&nbsp;Weixin Chen,&nbsp;Xueping Li,&nbsp;Xiaoyang Zhu","doi":"10.1016/j.stress.2024.100682","DOIUrl":"10.1016/j.stress.2024.100682","url":null,"abstract":"<div><div>Benzothiadiazole (BTH) is a plant resistance inducer that effectively delays ripening and senescence in fruits. To understand its possible mechanism, transcriptomics and metabolomics analyses were performed on banana (<em>Musa</em> spp.) fruit treated with BTH after their inoculation with the fungal pathogen <em>Colletotrichum musae.</em> A total of 1,747 differentially expressed genes (DEGs) were identified, and 1,160 were up- and 587 downregulated between the BTH and control conditions. These DEGs were highly enriched in metabolic pathways related to disease resistance, such as phenylpropanoid biosynthesis, flavonoids, and starch and sucrose metabolism. A metabolome analysis identified 138 differentially accumulated metabolites (DAMs) of which 73 were upregulated and 65 downregulated between the BTH and control conditions. Flavonoids were significantly upregulated DAMs. Transcriptome and metabolome analyses showed that the lignin biosynthesis and flavonoid biosynthesis pathways played important roles in the disease resistance of banana fruit induced by BTH by upregulating the expression of <em>PAL, 4CL, PER, CHS, FLS</em>, and <em>FL3H</em>. Moreover, WRKY transcription factors (TFs) also played key roles in this process of resistance by mediating genes related to disease resistance, such as <em>PR1, PR1c, TLPH, PTI5,</em> and other genes. Our results suggested that the BTH treatment significantly changed the expression of transcripts and metabolites related to the hormone signaling, secondary metabolism, disease resistance, and key TFs, which induced the disease resistance and delayed the ripening process. The insights into these molecular mechanisms also provide potential targets for genetic or chemical approaches to further enhance fruit quality and resistance.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100682"},"PeriodicalIF":6.8,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought is a lesser evil than cold for photosynthesis and assimilation metabolism of maize","authors":"Kamirán Á. Hamow ,&nbsp;Kinga Benczúr ,&nbsp;Edit Németh,&nbsp;Csaba Éva,&nbsp;Krisztina Balla,&nbsp;Magda Pál,&nbsp;Tibor Janda,&nbsp;Imre Majláth","doi":"10.1016/j.stress.2024.100669","DOIUrl":"10.1016/j.stress.2024.100669","url":null,"abstract":"<div><div>Cold is one of the abiotic environmental factors that severely affect plant metabolism. It causes changes in the fluidity of biological membranes, induces oxidative and osmotic stress, photoinhibition, reduces photosynthetic rates and slows down numerous metabolic enzyme activities. Some effects of cold overlap with drought response processes. Drought itself has a dualistic effect on plants. Severe drought stress is detrimental, while a mild preceding drought may prepare the otherwise sensitive maize to cope with cold. This study focused on the effects of mild drought on photosynthesis, sugar and amino acid metabolism at low temperature. Maize (<em>Zea mays</em> L.) plants were drought-hardened (15±5% relative soil moisture) between stages V4 and V6 at 25°C for 10 days and then subjected to cold treatmet at 10°C for 2 weeks at two light levels (PPFD=450 and 150 µmol m^-2 s^-1). The control population was watered daily (35±5% soil moisture), and in completely dried pots, 5±3% soil moisture was measured. The present results confirmed that photosynthetic performance and glutamine biosynthesis were positively affected by drought under both light intensities. Plants exposed to combined cold and moderate drought stress had a higher dry weight than those exposed to cold stress alone. The metabolism of several sugars, organic acids and amino acids was maintained by mild drought acclimation. The cold protective DHN2-like dehydrin gene was induced by moderate drought treatment, which may have contributed to the drought-induced higher tolerance to low temperature. However, cold stress markers were lower in low light than in normal growth light under drought acclimation, suggesting that drought-induced stress defense may require a certain level of light.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100669"},"PeriodicalIF":6.8,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite profiling in ten bread wheat (Triticum aestivum L.) genotypes in response to drought stress","authors":"Makhubu FN ,&nbsp;Mutanda M ,&nbsp;Madala NE ,&nbsp;Figlan S","doi":"10.1016/j.stress.2024.100680","DOIUrl":"10.1016/j.stress.2024.100680","url":null,"abstract":"<div><div>Wheat is frequently constrained by extreme environmental conditions such as drought. Improving drought tolerance in wheat genotypes is crucial for ensuring food security, especially considering the challenges posed by climate change. To reveal the involvement of metabolites in drought response, ten diverse wheat genotypes were investigated under control and water scarcity conditions. The field experiments were set-up, using a 5 × 2 alpha lattice design, with two replicates per treatment, in the 2022 and 2023 growing seasons. Metabolites associated with drought tolerance were analysed using ultra-high performance liquid chromatography, coupled with a quadruple time of flight mass spectrometry (UHPLC-qTOF-MS). Multivariate statistical analysis (MVDA) tools, viz. principal component analysis (PCA) and the orthogonal projection to latent structures-discriminant analysis (OPLS-DA) loading scatter plot were used to identify the metabolites that are positively and negatively correlated to drought stress. Significant variation (<em>p</em> &lt; 0.05) among genotypes was observed, with 58 metabolites annotated, including phenolic acids, carbohydrates, and fatty acids. The annotated compounds were linked to thirteen most significant pathways, with one carbon metabolism, cutin, suberin and wax synthesis and starch and sucrose metabolism being significantly affected by water stress, based on the KEGG pathway analysis. The two high-yielding wheat genotypes (LM48 and BW140) under drought stress displayed significant upregulation of key metabolites such as sinapoyl hydroxyagmatine, 7-oxostigmasterol, 1-O-caffeoyl-3-O-p-coumaroylglycerol, and 3-beta-3-lupanol, when compared to the non-stressed conditions. This study demonstrates the prospects of applied metabolomics for chemotaxonomic classification, phenotyping and selection in plant breeding, as well as potential use in crop improvement.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100680"},"PeriodicalIF":6.8,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dynamic transcriptome reveals response patterns to black shank disease in tobacco (Nicotiana tabacum L.)","authors":"Zhijun Tong ,&nbsp;Zuoqian Fan ,&nbsp;Tianyu Du ,&nbsp;Dunhuang Fang ,&nbsp;Xueyi Sui ,&nbsp;Chuyu Ye ,&nbsp;Qian-Hao Zhu ,&nbsp;Longjiang Fan ,&nbsp;Bingguang Xiao ,&nbsp;Enhui Shen","doi":"10.1016/j.stress.2024.100676","DOIUrl":"10.1016/j.stress.2024.100676","url":null,"abstract":"<div><div>Black shank disease, caused by <em>Phytophthora nicotianae</em>, is one of the major causes of yield loss in tobacco production. The present study aimed to explore the dynamic transcriptome in tobacco genotypes resistant or susceptible to black shank disease and to understand the defense response of tobacco to <em>P. nicotianae</em> infection. Roots and stems were sampled from two resistant and two susceptible materials at 0, 12, 24, 48, and 72 h post infection and used in RNA-sequencing. Conventional approaches that identify differentially expressed genes are not the best way for handling the complex datasets, so a new method that calculates the standard deviation among samples was applied to identify transcripts with variable expression levels in roots and stems of the four materials at different time points. Of the total of 229,501 transcripts, 7,261 were found to be variable transcripts, with many of them annotated to be related to defense responses against pathogen infection. These variable transcripts showed expression patterns that varied significantly between roots and stems as well as between the resistant and the susceptible materials. Several transcripts were identified to be potential candidates for further functional characterization. Our findings provide invaluable insights into the dynamic transcriptome in defense responses of tobacco against <em>P. nicotianae</em> infection.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100676"},"PeriodicalIF":6.8,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigating cypermethrin stress in Amaranthus hybridus L.: Efficacy of foliar-applied salicylic acid on growth, enzyme activity, and metabolite profiles","authors":"Arun Kumar,&nbsp;Pradeep Kumar Yadav,&nbsp;Anita Singh","doi":"10.1016/j.stress.2024.100673","DOIUrl":"10.1016/j.stress.2024.100673","url":null,"abstract":"<div><div>The study aimed to evaluate the role of foliar-applied salicylic acid (SA) (5-50 µM) in mitigating the effects of cypermethrin (CYP) stress on <em>Amaranthus hybridus</em> L., at its recommended (100 PPM) and double dose (200 PPM). CYP application at recommended dose proliferated the growth of the crop, while the double dose affected negatively. SA boosted the performance of the CYP-treated plants by reducing oxidative radical formation via increasing the activity of catalase, superoxide dismutase, peroxidase, ascorbate peroxidase, glutathione <em>s</em>-transferase, dehydroascorbate reductase, ascorbate, and proline. SA increased photosynthetic activity by increasing the leaf area, chlorophyll content and regulating the level of intracellular CO₂, stomatal conductance, and transpiration in the CYP-treated plants. The fresh weight of the crop showed maximal increase with 10 µM SA (57 %) at recommended dose and with 20 µM SA (36.8 %) at double dose of CYP. Additionally, CYP residues showed maximum decline with 10 µM and 20 µM SA application in 100P and 200P plants, respectively. UHPLC-MS analysis showed increased levels of crucial secondary metabolites such as antheraxanthin, violaxanthin, kaempferol, rutin, cinnamic acid, ferulic acid, trigonelline, coumarin, scoparone, esculin, caryophyllene oxide, jasmonal with SA supplementation at 10 µM with 100P and at 20 µM with 200P treatments. The observed changes in enzyme activities, physiology and metabolite profiles underscore the potential of SA in mitigating pesticide-induced stress and enhancing plant growth and health. This study could further be explored in future by combining SA with other phytohormones and applying omics approaches could enhance crop resilience across diverse environments.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100673"},"PeriodicalIF":6.8,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fascinating role of nanosilica in mitigating drought and nutrient stress – A review","authors":"Bekkam Rakesh ,&nbsp;Chitdeshwari T ,&nbsp;Mohanapriya G","doi":"10.1016/j.stress.2024.100672","DOIUrl":"10.1016/j.stress.2024.100672","url":null,"abstract":"<div><div>Drought stress is a major global challenge that severely impacts plant growth, photosynthesis and nutrient uptake, leading to significant yield losses. This necessitates the need for developing new agricultural technologies and one such advancement is nanotechnology. Recently, nanosilica has gained importance due to its significant role in mitigating drought and nutrient stress. The foliar/soil application and seed priming with nanosilica has shown to have positive impacts on plants under drought and nutrient stress by modulating morphological, physiological and biochemical parameters. This review aims to explore the impact of nanosilica in enhancing drought and nutrient stress tolerance in plants by demonstrating its beneficial effects on growth, gas exchange attributes, plant water status, membrane stability, antioxidant activity and silicon mediated uptake of nutrients. Further it also provides an overview of recent developments in nanosilica nutrition of crops and suggests future research directions to understand the role of nanosilica in alleviating drought and nutrient stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100672"},"PeriodicalIF":6.8,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the role of biopriming and nanopriming on the morphometric, biochemical, and yield parameters of Chickpea (Cicer arietinum L.) under drought stress","authors":"Simran Rani ,&nbsp;Priyanka Dahiya ,&nbsp;Aarzoo Sharma ,&nbsp;Yash Vashisth ,&nbsp;Kiran Arora ,&nbsp;Amita Suneja Dang ,&nbsp;Pooja Suneja","doi":"10.1016/j.stress.2024.100675","DOIUrl":"10.1016/j.stress.2024.100675","url":null,"abstract":"<div><div>This study evaluated drought mitigation potential of biopriming with plant growth promoting endophytic bacteria (PGPEBs) and nanopriming with their biological copper nanoparticles (CuNPs) and chemical CuNPs under polyethylene glycol (PEG-6000) induced moderate (MD-5%) and severe drought (SD-10%) in chickpea (<em>Cicer arietinum</em> L.). The crop harvested at 15 DDS (Days to drought stress) was analysed for morphometric and biochemical parameters of drought tolerant (DT), BG-4958 and drought sensitive (DS), ICC-1882 chickpea varieties. In morphometric traits of DT variety, N₁X led 384% increase in shoot dry weight (SDW) under MD while B₂ in root dry weight (RDW) under SD (418%). For DS variety, N₂X led 444% and 727% increase in SDW (MD) and RDW (SD), respectively. Amongst biochemical parameters, maximum increment was noticed in total chlorophyll content (TCC) by B₁ under MD (703%) as well as SD (1206%) in DT variety. B₁ also led highest increment (758%) in TCC of DS variety under SD while B₂ under MD (300%). B₂ resulted in 242% increment in total soluble carbohydrates (TSC) and 47% increase in total protein content (TPC) of DS variety under SD. N₁X and N₁Y led 318% and 100% increase in the activity of ascorbate peroxidase (APX) and peroxidase (POD) of DS variety. This variety exhibiting pronounced response was subjected to correlation analysis revealing highest correlation amongst morpho-biochemical traits under SD. Score plot in principal component analysis (PCA) of DS variety showed that biopriming and N₁X having higher score values for PC₂ mainly influenced by biochemical parameters also improved the yield parameters to a greater extent as analyzed on 120 DDS. Harvesting index, the ultimate indicator of the agricultural output remained insignificant in DT variety. On the other hand, B₂ and N₁X led highest harvesting indices under MD (94%) and SD (69%), respectively, in DS variety, owing to their higher grain yield than biological yield and higher score values influencing biochemical parameters under stress. The present study provides insights into the beneficial role of PGPEBs and biosynthesized CuNPs in mitigating the adverse effects of drought in chickpea.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100675"},"PeriodicalIF":6.8,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An insight to strategical responses of particulate pollution in plants: From phenome to genome","authors":"Soumya Chatterjee ,&nbsp;Mamun Mandal ,&nbsp;Mrinalini Kakkar ,&nbsp;Ganapati Basak ,&nbsp;Nasrin Banu Khan ,&nbsp;Ranadhir Chakraborty ,&nbsp;Robert Popek ,&nbsp;Abhijit Sarkar ,&nbsp;Chandan Barman","doi":"10.1016/j.stress.2024.100671","DOIUrl":"10.1016/j.stress.2024.100671","url":null,"abstract":"<div><div>Particulate matter (PM) is an extremely overlooked air pollutant with drastic effects on the biome, owing to the industrial and agricultural advancements, significantly exacerbating global environmental contamination levels. The altered atmosphere in urban settings due to PM pollution profoundly influences plants' morphological, physiochemical state and allied responses. PM exposure leads to drastic decrease in plant-height, phytomass, leaf number, leaf length and productivity. PM change the epicuticular wax patterns, penetrates plant tissue through stomata, and denatures the chloroplast pigmentation. It changes leaves' light absorption and reflection patterns, weakening the total radiation that reaches the chlorophyll antenna and ultimately reducing the photosynthetic rate and electron transport chain. Consequently, this alters plants morphology like wax deposits, thick epidermis, and long trichomes near stomata. Moreover, PM stress also adversely effects gluconeogenesis, amino acid biosynthesis, TCA cycle, and photorespiration-associated gene expression. Several transcription factors, such as <em>MYB, C3H</em>, and <em>G2</em>-homologues, are activated as a collective stress response. Additionally, ascorbic acid, proline and soluble sugars accumulate and several antioxidants are produced to scavenge the PM-induced reactive oxygen species (ROS). This review aims to document plants' various responses to PM pollution in their respective eco-geographic settings and investigate ways used by plants to mitigate PM pollution. We also enumerate the consequences of PM pollution on plants and the corresponding phenomic and genetic mechanisms through which plants adapt.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100671"},"PeriodicalIF":6.8,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought-mediated oxidative stress and its scavenging differ between citrus hybrids with medium and late fruit maturation","authors":"Jin Jia,&nbsp;Mingjiong Zhao,&nbsp;Rui Liu,&nbsp;Caixin Xue,&nbsp;Zhuyuan Xia,&nbsp;Bin Hu,&nbsp;Heinz Rennenberg","doi":"10.1016/j.stress.2024.100670","DOIUrl":"10.1016/j.stress.2024.100670","url":null,"abstract":"<div><div>Drought stress is a major environmental factor limiting citrus productivity. Still, differences in drought sensitivity between citrus hybrids of different maturation periods have so far not been reported. Here, we selected a medium-maturing (Fertile orange: FO (<em>Citrus reticulata</em> cv. Fertile orange) and a late-maturing citrus hybrid (Newhall Navel orange: NO (<em>Citrus sinensis</em> Osbeck cv. Newhall) and determined the physiological and biochemical traits of leaves, roots, wood and bark. Our results showed that drought significantly decreased net photosynthetic rate (<em>Pn</em>), stomatal conductance (<em>Gs</em>) and transpiration rate (<em>Tr</em>) of citrus leaves. Oxidative stress upon drought was indicated by enhanced foliar malondialdehyde (MDA) and hydrogen peroxide contents, as well as a stimulation of the anti-oxidative system. This stimulation included the contents of dehydroascorbic acid (DHA), glutathione (GSH) and oxidized glutathione (GSSG) in leaves, roots, wood and bark, as well as activities of antioxidative enzymes of glutathione reductase (GR), dehydroascorbate reductase (DHAR), superoxide dismutase (SOD) and peroxidase (POD). The late maturing NO hybrid not only showed better general physiological performance as indicated by increased <em>Pn</em> in leaves, but also higher biochemical ROS scavenging and osmotic capacity as indicated by increased ascorbic acids (ASA), DHA, and proline contents, as well as activities of enzymes of SOD, POD, ASA/DHA and GSH/GSSG ratios in the investigated tissues compared to the FO hybrid under drought and control conditions. Analysis of molecular mechanisms of signaling, regulatory and functional genes expression are suggested for future studies to elucidate the complex interplay of molecular, biochemical and physiological responses of citrus hybrids to drought.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100670"},"PeriodicalIF":6.8,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C and N allocation on wheat under the effects of depleted, current and elevated [CO2] are modulated by water availability","authors":"Salvador Aljazairi ,&nbsp;Brigen Manikan ,&nbsp;Xavier Serrat ,&nbsp;Salvador Nogués","doi":"10.1016/j.stress.2024.100663","DOIUrl":"10.1016/j.stress.2024.100663","url":null,"abstract":"<div><div>Although elevated atmospheric [CO₂] has substantial indirect effects on vegetation carbon uptake via associated climate change, their dynamics remain unclear. The carbon and nitrogen allocation and partitioning in durum wheat were compared at different [CO₂] and different water availability. The aim of this study was to investigate how the impacts of depleted and elevated [CO₂] driven climate change on Mediterranean wheat plants under drought conditions. For that reason, double stable isotope labelling using ¹³CO₂ and ¹⁵NH₄–¹⁵NO₃ was conducted to follow ¹³C and ¹⁵N allocation and partitioning in the different plant organs. Plants were studied in growth chambers under three different CO₂ environments (depleted, current and elevated) and two water availability conditions (well-watered and mild-water-stress). Isotopic ¹³C and ¹⁵N determination, gas exchange analyses and growth parameters were measured.</div><div>We show that plants subjected to depleted and elevated [CO₂] suffered up and down regulation of photosynthesis respectively, but their responses were both modulated by water availability. Depleted [CO₂] and drought reduced plant biomass. However, elevated [CO₂], show that the initial positive effect of elevated [CO₂] on carbon uptake declined rapidly, showing a consequence of physiological acclimation and the inhibition of [Rubisco] and activity, this effect was more evident in combination with drought. In both cases, depleted [CO₂] and elevated [CO₂] condition modified the C and N allocation compared with current [CO₂], overall combined with drought.</div><div>These results obtained highlight the different C and N management strategies of wheat and provide relevant information about the potential response of plants under global climate change conditions.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100663"},"PeriodicalIF":6.8,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142699481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}