Plant Stress最新文献

{"title":"The interaction between BnaAIF1 and BnaICE1 enhances the low-temperature tolerance of Brassica napus","authors":"Xiaoyan Xia ,&nbsp;Shun Li ,&nbsp;Lei Sun,&nbsp;Zhonghua Wang,&nbsp;Xiaoyu Chen,&nbsp;Bo Yang,&nbsp;Zixuan Zhou,&nbsp;Xin He","doi":"10.1016/j.stress.2025.101053","DOIUrl":"10.1016/j.stress.2025.101053","url":null,"abstract":"<div><div>Rapeseed (<em>Brassica napus</em> L.) is an important oilseed crop in the world, it’s often damaged by low-temperature (especially freezing) stress, which has the potential to cause significant yield losses. However, little is known about the molecular mechanisms for coping with low-temperature stress in rapeseed. In this study, a total of 24 atypical bHLH transcription factor <em>AIF</em> (ATBS1 INTERACTING FACTOR) gene family members were identified by systematically bioinformatics analysis in rapeseed, among which all six <em>BnaAIF1</em> genes were strongly induced by low temperature stress, especially <em>BnaAIF1-A03/C03</em>. Overexpression of <em>BnaAIF1-C03</em> improved the seed germination under cold stress (4 °C) and the survival rate of seedlings under freezing stress (-4 °C) in rapeseed, with enhanced stability of light system II (Fv/Fm and NPQ_Lss increased) and reduced oxidative damage (decreased contents of H₂O₂ and MDA). Further analysis revealed that BnaAIF1-C03 was localized in the nucleus and interacted with BnaICE1, activating the ICE1-CBF-COR pathway genes and thus participating in the low-temperature stress response of rapeseed. Overall, this study provides an important theoretical basis and new target gene for future breeding of low-temperature tolerant rapeseed.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101053"},"PeriodicalIF":6.8,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145157790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaping plant resilience through nitrate nutrition: Insights from the crosstalk between nitrate signaling and stress response","authors":"Jingjing Mao,&nbsp;Jinhao Sun,&nbsp;Zhen Tian,&nbsp;Duanfei Wang,&nbsp;Yating Yu,&nbsp;Shaopeng Li","doi":"10.1016/j.stress.2025.101047","DOIUrl":"10.1016/j.stress.2025.101047","url":null,"abstract":"<div><div>Environmental stresses are a constant challenge for plants and a key determinant of how plants respond to these stressors is their nutritional status, particularly the availability of essential nutrients such as nitrate (NO₃^-). Recent research has uncovered a complex crosstalk between nitrate signaling pathways and stress response. In addition to being a key macronutrient, NO₃^- acts as a signaling molecule that modulates various aspects of plant metabolism and defense. This review explores two layers of interaction between nitrate signaling and plant resilience: indirect crosstalk mediated by the regulation of interconnected physiological processes, and direct crosstalk via specific regulatory proteins. By examining how NO₃^- availability influences stress responses, we aim to provide new insights into the mechanisms underlying plant resilience. This understanding holds significant potential for promoting more sustainable agricultural practices and enhancing crop performance under stress conditions.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101047"},"PeriodicalIF":6.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of phenology and agronomical performance of Syrah grafted on two rootstocks under combined salinity and water stress conditions: A three-year field study","authors":"Kidanemaryam Reta ,&nbsp;Yaniv Lupo ,&nbsp;Noga Sikron Persi ,&nbsp;Naftali Lazarovitch ,&nbsp;Aaron Fait","doi":"10.1016/j.stress.2025.101050","DOIUrl":"10.1016/j.stress.2025.101050","url":null,"abstract":"<div><div>Climate change is challenging global viticulture through increasing drought and salinization, making rootstock selection critical. However, field-based understanding of rootstock-mediated stress responses remains limited. Here, we examined the mediation of Sélection Oppenheim 4 (SO4) and 1103 Paulsen (PL1103) rootstocks on Syrah grapevines' responses under combined water and salinity stress over 3 years (2022‒2024). We observed that the soil electrical conductivity (EC_e1:1) increased from 2.09 to 8 dS <em>m</em>⁻¹ under severe stress, while soil chloride concentration ([Cl⁻]) reached 716.5 mg <em>L</em>⁻¹ in Syrah grafted onto PL1103 (SY_PL1103) and 954.9 mg <em>L</em>⁻¹ in Syrah grafted onto SO4 (SY_SO4), compared with 90‒162 mg L^‒1 under the control conditions coupled with varied leaf [Cl⁻] between grafts. Salinity delayed flowering time by 7‒11 days when the EC_e1:1 exceeded 2.79 dS m^‒1 in SY_PL1103 and 1.99 dS m^‒1 in SY_SO4. Nevertheless, SY_SO4 maintained greater physiological performance with 56.8 % greater photosystem II efficiency, 48.71 % greater electron transport rate, and higher non-photochemical quenching across conditions than SY_PL1103, effectively managing excess light as heat to prevent photodamage. SY_SO4 vines exhibited a significantly lower yield reduction (36.3 % vs 56.4 % in SY_PL1103) and better salinity tolerance, with yield decreasing by only 2.63 t ha⁻¹ per unit increase in EC_e1:1 above the threshold (SY_PL1103 = 3.47 dS <em>m</em>⁻¹ and SY_SO4 = 2.71 dS <em>m</em>⁻¹) compared with 7.78 t ha⁻¹ in SY_PL1103. SY_SO4 showed smaller photosynthetic and morphological changes to combined stressors, with higher soil and lower leaf [Cl−] indicating better ion exclusion, offering valuable insights and practical solutions for resilient grapevine breeding and vineyard management strategies in saline-prone arid regions.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101050"},"PeriodicalIF":6.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated physiological, transcriptomic, and metabolomic analyses reveal the response mechanism of the CsNADP-ME4 gene to drought stress in cucumber (Cucumis sativus L.)","authors":"Putao Wang ,&nbsp;Jiali Lin ,&nbsp;Zijin Xiang ,&nbsp;Xia Zou ,&nbsp;Sha Luo ,&nbsp;Yao Xiao ,&nbsp;Jingyu Sun ,&nbsp;Shenglin Wang ,&nbsp;Qianglong Zhu ,&nbsp;Cuixiang Huang ,&nbsp;Qinghong Zhou ,&nbsp;Nan Shan","doi":"10.1016/j.stress.2025.101048","DOIUrl":"10.1016/j.stress.2025.101048","url":null,"abstract":"<div><div>Drought is a pervasive abiotic stress that poses a significant threat to global vegetable production. Although the involvement of NADP-malic enzyme (NADP-ME) in plant drought resistance is documented, its specific function and regulatory mechanisms in cucumber remain underexplored. In this study, the NADP-ME gene <em>CsNADP-ME4</em> was predominantly expressed in cucumber leaves and was inducible by drought stress. Subcellular localization confirmed the chloroplast targeting of CsNADP-ME4. Functional analysis using RNA interference (RNAi) indicated that downregulating <em>CsNADP-ME4</em> increased drought sensitivity, as evidenced by severe leaf wilting, reduced photosynthetic efficiency, and altered activities of osmotic regulators and protective enzymes compared to wild-type plants. Integrated transcriptomic and metabolomic analyses revealed that <em>CsNADP-ME4</em> silencing disrupts the levels of sugars, amino acids, and phytohormones, alongside extensive changes in gene expression. Promoter analysis identified MYB-binding <em>cis</em>-elements, and subsequent experiments, including yeast one-hybrid, dual luciferase reporter, and electrophoretic mobility shift assays, confirmed that the transcription factor CsMYB16 directly binds to the <em>CsNADP-ME4</em> promoter to activate its transcription. In conclusion, these results emphasize the critical role of <em>CsNADP-ME4</em> in enhancing drought resilience and suggests a broader function for MYB transcription factors in regulating stress-responsive genes. These findings provided precise targets and genetic resources for molecular design breeding of cucumbers, and offered a solid theoretical basis for the development of new cucumber varieties that are drought-tolerant.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101048"},"PeriodicalIF":6.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis","authors":"Emmanuel Flores-Hernández ,&nbsp;María Elisa Gonzalez ,&nbsp;Paulina Alvarado-Guitron ,&nbsp;Francisco I. Jasso-Robles ,&nbsp;Cesaré Ovando-Vázquez ,&nbsp;Juan Francisco Jiménez-Bremont ,&nbsp;Sanja Ćavar Zeljković ,&nbsp;Markéta Ulbrichová ,&nbsp;Nuria De Diego ,&nbsp;Margarita Rodríguez-Kessler","doi":"10.1016/j.stress.2025.101046","DOIUrl":"10.1016/j.stress.2025.101046","url":null,"abstract":"<div><div>The study of polyamine transport in plants has become increasingly important due to the central role of these amines in regulating growth, development, adaptation, and stress responses. This research focused on the <em>Arabidopsis thaliana Polyamine Uptake Transporters</em> gene family under conditions of systemic acquired resistance. We evaluated all single mutants of this gene family and found that the <em>put2-1</em> mutant abolished systemic acquired resistance while enhancing basal resistance to <em>Pseudomonas syringae</em> pv. <em>tomato</em> DC3000. In contrast, the <em>35S::PUT2</em> overexpression lines showed improved resistance and reduced bacterial titers compared to wild-type plants. RNA-seq analysis revealed that the <em>put2-1</em> mutant had deregulated expression of genes involved in the biosynthesis, signaling, and inactivation of salicylic acid and N-hydroxypipecolic acid. Most of these genes were transcriptionally upregulated by putrescine in wild-type plants, but not in the <em>put2-1</em> mutant. Putrescine supplementation increased endogenous putrescine and salicylic acid levels in wild-type plants but not in <em>put2-1</em>, highlighting the essential role of this transporter in facilitating putrescine mobilization and regulating salicylic acid in distal tissues. We found that the defective systemic acquired resistance phenotype in the <em>put2-1</em> mutant was linked to changes in the timing of polyamines, ROS, phenolic compound accumulation, and alterations in stomatal immunity. Our study emphasizes the key role of the Polyamine Uptake Transporter 2 (PUT2/LAT4) in establishing systemic acquired resistance in <em>Arabidopsis</em>, while also maintaining the plant’s intrinsic basal resistance mechanisms. These findings offer valuable insights into the complex mechanisms of plant resistance, positioning polyamine transport as a central hub in systemic responses.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101046"},"PeriodicalIF":6.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellulose synthase superfamily key in DAMPs-triggered immunity against Sclerotinia stem rot in Brassica napus","authors":"Wennian Xia ,&nbsp;Yunli Su ,&nbsp;Jiayi Song ,&nbsp;Li Wang ,&nbsp;Meiyun Yang ,&nbsp;Jie Yang ,&nbsp;Ran Zhang ,&nbsp;Xiaomao Cheng ,&nbsp;Feng Zu ,&nbsp;Huizhen Hu","doi":"10.1016/j.stress.2025.101040","DOIUrl":"10.1016/j.stress.2025.101040","url":null,"abstract":"<div><div>The cellulose synthase (CESA) superfamily, critical for cell wall biosynthesis and immunity, is poorly characterized in <em>Brassica napus</em> resistance to Sclerotinia stem rot (SSR). We genome-wide identified 92 <em>BnCESA</em> superfamily genes, phylogenetically clustered into seven subfamilies (BnCESA, BnCSLA-BnCSLG). Structural analysis revealed conserved catalytic (DxD, TED) and regulatory (QxxRW) domains polysaccharide synthesis and immune responses. Intraspecific synteny analysis revealed non-uniform gene distribution across chromosomes, while interspecific comparison with <em>Arabidopsis thaliana, Brassica rapa, Brassica oleracea</em>, and <em>Oryza sativa</em> showed high collinearity with Brassica species (75–159 syntenic pairs) but limited conservation with rice (2 pairs), underscoring evolutionary conservation within Brassicaceae. Promoter <em>cis</em>-elements showed enrichment of jasmonic acid (JA)- and salicylic acid (SA)-responsive motifs, aligning with infection-stage expression dynamics. Transcriptomic profiling identified 36 infection-responsive genes, with 15 core candidates (e.g., <em>BnCSLG2c, BnCESA3b, BnCSLD3d, BnCSLC8a</em>) driving biphasic defense: SA-mediated early responses (6–24 hpi) transitioning to JA-dominated late resistance (36–60 hpi), reducing lesion expansion by up to 50 %. Oligosaccharide-derived damage-associated molecular patterns (DAMPs)—cellobiose (CB), xylooligosaccharides (XOS), and oligogalacturonides (OG), activated distinct programs: CB induced sustained responses (0–60 hpi), OG prioritized wall remodeling (48–60 hpi), and XOS synchronized with circadian-regulated defenses. Molecular docking and comparative expression profiling in resistant/susceptible rapeseed varieties predicted seven core genes (<em>BnCSLG2c, BnCSLC8a, BnCESA6b, BnCESA6a, BnCESA5b, BnCESA3b</em>, and <em>BnCSLD3d</em>) as potential integrators of cell wall integrity and immune signaling. Our finding provides potential genetic resources for precision breeding of SSR-resistant rapeseed.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101040"},"PeriodicalIF":6.8,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"B-BOX PROTEINS (BBXs) transcription factors in plant: A comprehensive review of their growth, development, abiotic stress responses and phytohormone signals","authors":"Yaxuan Jiang ,&nbsp;Pei Lei ,&nbsp;Ximei Ji ,&nbsp;Jianxin Li ,&nbsp;Yong Zhao ,&nbsp;Mingquan Jiang ,&nbsp;Le Ma ,&nbsp;Xiyang Zhao ,&nbsp;Fanjuan Meng","doi":"10.1016/j.stress.2025.101042","DOIUrl":"10.1016/j.stress.2025.101042","url":null,"abstract":"<div><div>Global climate change exacerbates the effects of environmental stressors, such as drought, temperatures, salinity. Over the past two decades, there have been many studies on B-BOX PROTEINS (BBXs) in plants, with much of the research concentrated on their roles in plant growth and development. Nevertheless, recent findings have revealed that BBXs are also required for environmental stressors. In this review, we survey recent advances in the characterization BBXs of molecular mechanisms in abiotic stress tolerance and plant growth. The interaction between BBXs transcription factor and related proteins and the binding to promoter elements of stress response related genes were emphatic discussed. In addition, we discuss the future challenges and opportunities for extend the BBXs knowledge model plant to other species, and provide a better understanding of plant growth and development in natural conditions.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101042"},"PeriodicalIF":6.8,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suitable light intensity stimulated polysaccharide biosynthesis in bletilla striata pseudobulbs through regulating starch and sucrose metabolism","authors":"Jiao Zhu ,&nbsp;Liuyan Yang ,&nbsp;Youming Cai ,&nbsp;Xinhua Zeng ,&nbsp;Yongchun Zhang ,&nbsp;Weichang Huang","doi":"10.1016/j.stress.2025.101041","DOIUrl":"10.1016/j.stress.2025.101041","url":null,"abstract":"<div><div><em>Bletilla striata</em> is a famous medicinal plant, which medicinal function on polysaccharide. However, the effect of light intensity on polysaccharide accumulation in <em>B. striata</em> pseudobulbs is largely unknown. <em>B. striata</em> plants were exposed to three different light intensities: low light (5–20 μmol·<em>m</em>⁻²·s⁻¹; LP), middle light (200 μmol·<em>m</em>⁻²·s⁻¹; MP), and high light (400 μmol·<em>m</em>⁻²·s⁻¹; HP). The results indicated that polysaccharide content of new pseudobulbs significantly increased under MP, and enrichment in starch and sucrose metabolism with metabolomics and transcriptomics. More sucrose was produced in the leaves through photosynthesis and was efficiently distributed to the pseudobulbs, where it was hydrolyzed into key metabolites for polysaccharide biosynthesis function by <em>SUS</em> and <em>CSLA</em> genes. The key metabolites in the polysaccharide biosynthetic pathway, such as sucrose-6-phosphate, fructose, fructose-6-phosphate, glucose-6-phosphate, GDP-mannose, and uridine 5′-diphospho-<span>d</span>-glucose, were upregulated under MP. Furthermore, the expression of the <em>UGP2</em> gene involved in polysaccharide biosynthesis was significantly higher under MP than under LP and HP. Conversely, sucrose in leaves was minimally transported into pseudobulbs due to insufficient photosynthesis under LP and energy metabolism related to carbohydrate degradation and oxidation was hindered under HP. Thus, suitable light intensity effectively stimulated polysaccharide formation in <em>B. striata</em> pseudobulbs through starch and sucrose metabolism–mediated regulation.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101041"},"PeriodicalIF":6.8,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145157663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon ion beam-induced radiation hormesis in Bupleurum chinense DC.: Insights from growth, physiological, and metabolomic analyses for increased bioactive substances","authors":"Xiao Liu ,&nbsp;Man Wang ,&nbsp;Yuanmeng Wang ,&nbsp;Xuehu Li ,&nbsp;Fusheng Wang ,&nbsp;Zhijun Xin ,&nbsp;Xihong Lu ,&nbsp;Xiaochun Pan ,&nbsp;Li Li ,&nbsp;Yan Du ,&nbsp;Libin Zhou","doi":"10.1016/j.stress.2025.101037","DOIUrl":"10.1016/j.stress.2025.101037","url":null,"abstract":"<div><div><em>Bupleurum chinense</em> DC (<em>B. chinense</em>) is an important medicinal plant widely used in Asian countries. However, the efficacy of its medicinal components has medicinal components during the domestication process from wild to cultivated lines. Studies have shown that appropriate stress, such as ionizing radiation, can promote the accumulation of metabolites in medicinal plants. Nevertheless, the effects of ionizing radiation on <em>B. chinense</em> remains unclear. In this study, we systematically investigated the stimulatory effect of carbon ion beams (CIB) pretreatment on the growth, physiology, and accumulation of secondary metabolites in <em>B. chinense</em>. Although CIB irradiation inhibited the seed germination and survival rates, by the age of 4 months, the plant height and leaf area of the irradiated group had recovered to the levels comparable to the control. The enhanced growth performance during later developmental stages may be attributed to radiation hormesis and ROS-mediated regulation in antioxidant system and the photosynthetic system. One and two years after irradiation at doses of 50 and 100 Gy, no significant differences in root biomass were observed compared to the control group. Metabolically, the content of total flavonoid, saikosaponin a and saikosaponin c were significantly increased following 50 Gy irradiation. Further metabolomic analysis revealed that intermediate metabolites in the flavonoid and terpenoid biosynthetic pathways were significantly up-regulated in the 50 Gy irradiation group. Compounds with pharmacological activity also accumulated in large quantities after irradiation. These results suggest that pretreatment with 50 Gy CIB irradiation could serve as a potential method to promote the accumulation of secondary metabolites in <em>B. chinense</em>. This finding provides strong support for the application of physical radiation technology to enhance the production of secondary metabolites in medicinal plants, offering new avenues for the cultivation of high-quality Chinese herbal medicines.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101037"},"PeriodicalIF":6.8,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145157661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic reconfiguration and proline-mediated responses enhance citrus tolerance to combined water, light and heat stress","authors":"Damián Balfagón,&nbsp;Clara Segarra-Medina,&nbsp;José L. Rambla,&nbsp;Aurelio Gómez-Cadenas","doi":"10.1016/j.stress.2025.101039","DOIUrl":"10.1016/j.stress.2025.101039","url":null,"abstract":"<div><div>The co-occurrence of multiple abiotic stress factors has become increasingly common in agricultural systems, largely driven by the intensification of climate change. These overlapping stressors not only occur more frequently but also with greater intensity, posing complex challenges to crop resilience and overall productivity. In this context, the ability of plants to adjust their metabolism plays a pivotal role in facilitating acclimation to such multifaceted environmental pressures. The present study explores how two citrus rootstocks with differing stress tolerance profiles—Carrizo citrange and Cleopatra mandarin—reconfigure their primary metabolism when simultaneously exposed to water limitation, high light exposure, and elevated temperatures. Our results demonstrate that Carrizo exhibits distinct metabolic alterations under high temperature stress, whereas Cleopatra remains largely unaffected, suggesting an inherently lower heat tolerance in Cleopatra. Furthermore, Carrizo plants accumulate proline in response to the triple stress combination, while Cleopatra instead accumulates γ-aminobutyric acid (GABA). Remarkably, treatment with exogenous proline enhances stress tolerance in both genotypes by increasing antioxidant enzyme activities and activating autophagy-related stress response pathways. While previous studies have explored plant responses to individual stress factors, our research reveals that metabolic reconfiguration and proline accumulation are key to coping with the combined effects of water deficit, high irradiance, and extreme temperatures in citrus. These findings underscore the pivotal role of proline metabolism in mitigating the detrimental effects of complex abiotic stresses and point to potential strategies for improving citrus adaptation through metabolic modulation. Overall, our study provides valuable insights into the mechanisms driving plant responses to environmental challenges, which are essential for developing sustainable agricultural practices in the face of climate change.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101039"},"PeriodicalIF":6.8,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}