纤维素合成酶超家族在甘蓝型油菜抗菌核病免疫中的关键作用

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2025-09-22 DOI:10.1016/j.stress.2025.101040

Wennian Xia , Yunli Su , Jiayi Song , Li Wang , Meiyun Yang , Jie Yang , Ran Zhang , Xiaomao Cheng , Feng Zu , Huizhen Hu

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引用次数: 0

摘要

纤维素合成酶（CESA）超家族对细胞壁生物合成和免疫至关重要，但在甘蓝型油菜对菌核病（Sclerotinia stem rot， SSR）的抗性中尚不清楚。我们在全基因组范围内鉴定出92个BnCESA超家族基因，系统发育上聚集为7个亚家族（BnCESA, BnCSLA-BnCSLG）。结构分析显示保守的催化（DxD， TED）和调节（QxxRW）结构域多糖合成和免疫应答。与拟南芥、油菜、甘蓝和水稻的种间共线性分析显示，该基因与芸苔属植物的共线性较高（75-159对），与水稻的共线性较低（2对），表明该基因在芸苔科植物内部具有进化保守性。启动子顺式元件显示茉莉酸（JA）和水杨酸（SA）响应基序富集，与感染期表达动态一致。转录组学分析鉴定了36个感染应答基因，其中15个核心候选基因（例如BnCSLG2c， BnCESA3b, BnCSLD3d, BnCSLC8a）驱动双相防御：sa介导的早期应答（6-24 hpi）过渡到ja主导的晚期耐药（36 - 60 hpi），减少病变扩展高达50%。低聚糖衍生的损伤相关分子模式(DAMPs) -纤维素二糖（CB），低聚木糖（XOS）和低聚半乳糖醛酸酯（OG），激活不同的程序：CB诱导持续反应（0-60 hpi）， OG优先壁重塑（48-60 hpi）， XOS与生物钟调节的防御同步。分子对接和比较表达谱预测了7个核心基因（BnCSLG2c、BnCSLC8a、BnCESA6b、BnCESA6a、BnCESA5b、BnCESA3b和BnCSLD3d）作为细胞壁完整性和免疫信号传导的潜在整合子。本研究为油菜抗ssr的精准育种提供了潜在的遗传资源。

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Cellulose synthase superfamily key in DAMPs-triggered immunity against Sclerotinia stem rot in Brassica napus

The cellulose synthase (CESA) superfamily, critical for cell wall biosynthesis and immunity, is poorly characterized in Brassica napus resistance to Sclerotinia stem rot (SSR). We genome-wide identified 92 BnCESA superfamily genes, phylogenetically clustered into seven subfamilies (BnCESA, BnCSLA-BnCSLG). Structural analysis revealed conserved catalytic (DxD, TED) and regulatory (QxxRW) domains polysaccharide synthesis and immune responses. Intraspecific synteny analysis revealed non-uniform gene distribution across chromosomes, while interspecific comparison with Arabidopsis thaliana, Brassica rapa, Brassica oleracea, and Oryza sativa showed high collinearity with Brassica species (75–159 syntenic pairs) but limited conservation with rice (2 pairs), underscoring evolutionary conservation within Brassicaceae. Promoter cis-elements showed enrichment of jasmonic acid (JA)- and salicylic acid (SA)-responsive motifs, aligning with infection-stage expression dynamics. Transcriptomic profiling identified 36 infection-responsive genes, with 15 core candidates (e.g., BnCSLG2c, BnCESA3b, BnCSLD3d, BnCSLC8a) driving biphasic defense: SA-mediated early responses (6–24 hpi) transitioning to JA-dominated late resistance (36–60 hpi), reducing lesion expansion by up to 50 %. Oligosaccharide-derived damage-associated molecular patterns (DAMPs)—cellobiose (CB), xylooligosaccharides (XOS), and oligogalacturonides (OG), activated distinct programs: CB induced sustained responses (0–60 hpi), OG prioritized wall remodeling (48–60 hpi), and XOS synchronized with circadian-regulated defenses. Molecular docking and comparative expression profiling in resistant/susceptible rapeseed varieties predicted seven core genes (BnCSLG2c, BnCSLC8a, BnCESA6b, BnCESA6a, BnCESA5b, BnCESA3b, and BnCSLD3d) as potential integrators of cell wall integrity and immune signaling. Our finding provides potential genetic resources for precision breeding of SSR-resistant rapeseed.

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.