Xiao Liu , Man Wang , Yuanmeng Wang , Xuehu Li , Fusheng Wang , Zhijun Xin , Xihong Lu , Xiaochun Pan , Li Li , Yan Du , Libin Zhou
{"title":"碳离子束诱导的柴胡辐射激效。:生物活性物质增加的生长、生理和代谢组学分析","authors":"Xiao Liu , Man Wang , Yuanmeng Wang , Xuehu Li , Fusheng Wang , Zhijun Xin , Xihong Lu , Xiaochun Pan , Li Li , Yan Du , Libin Zhou","doi":"10.1016/j.stress.2025.101037","DOIUrl":null,"url":null,"abstract":"<div><div><em>Bupleurum chinense</em> DC (<em>B. chinense</em>) is an important medicinal plant widely used in Asian countries. However, the efficacy of its medicinal components has medicinal components during the domestication process from wild to cultivated lines. Studies have shown that appropriate stress, such as ionizing radiation, can promote the accumulation of metabolites in medicinal plants. Nevertheless, the effects of ionizing radiation on <em>B. chinense</em> remains unclear. In this study, we systematically investigated the stimulatory effect of carbon ion beams (CIB) pretreatment on the growth, physiology, and accumulation of secondary metabolites in <em>B. chinense</em>. Although CIB irradiation inhibited the seed germination and survival rates, by the age of 4 months, the plant height and leaf area of the irradiated group had recovered to the levels comparable to the control. The enhanced growth performance during later developmental stages may be attributed to radiation hormesis and ROS-mediated regulation in antioxidant system and the photosynthetic system. One and two years after irradiation at doses of 50 and 100 Gy, no significant differences in root biomass were observed compared to the control group. Metabolically, the content of total flavonoid, saikosaponin a and saikosaponin c were significantly increased following 50 Gy irradiation. Further metabolomic analysis revealed that intermediate metabolites in the flavonoid and terpenoid biosynthetic pathways were significantly up-regulated in the 50 Gy irradiation group. Compounds with pharmacological activity also accumulated in large quantities after irradiation. These results suggest that pretreatment with 50 Gy CIB irradiation could serve as a potential method to promote the accumulation of secondary metabolites in <em>B. chinense</em>. This finding provides strong support for the application of physical radiation technology to enhance the production of secondary metabolites in medicinal plants, offering new avenues for the cultivation of high-quality Chinese herbal medicines.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101037"},"PeriodicalIF":6.8000,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Carbon ion beam-induced radiation hormesis in Bupleurum chinense DC.: Insights from growth, physiological, and metabolomic analyses for increased bioactive substances\",\"authors\":\"Xiao Liu , Man Wang , Yuanmeng Wang , Xuehu Li , Fusheng Wang , Zhijun Xin , Xihong Lu , Xiaochun Pan , Li Li , Yan Du , Libin Zhou\",\"doi\":\"10.1016/j.stress.2025.101037\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Bupleurum chinense</em> DC (<em>B. chinense</em>) is an important medicinal plant widely used in Asian countries. However, the efficacy of its medicinal components has medicinal components during the domestication process from wild to cultivated lines. Studies have shown that appropriate stress, such as ionizing radiation, can promote the accumulation of metabolites in medicinal plants. Nevertheless, the effects of ionizing radiation on <em>B. chinense</em> remains unclear. In this study, we systematically investigated the stimulatory effect of carbon ion beams (CIB) pretreatment on the growth, physiology, and accumulation of secondary metabolites in <em>B. chinense</em>. Although CIB irradiation inhibited the seed germination and survival rates, by the age of 4 months, the plant height and leaf area of the irradiated group had recovered to the levels comparable to the control. The enhanced growth performance during later developmental stages may be attributed to radiation hormesis and ROS-mediated regulation in antioxidant system and the photosynthetic system. One and two years after irradiation at doses of 50 and 100 Gy, no significant differences in root biomass were observed compared to the control group. Metabolically, the content of total flavonoid, saikosaponin a and saikosaponin c were significantly increased following 50 Gy irradiation. Further metabolomic analysis revealed that intermediate metabolites in the flavonoid and terpenoid biosynthetic pathways were significantly up-regulated in the 50 Gy irradiation group. Compounds with pharmacological activity also accumulated in large quantities after irradiation. These results suggest that pretreatment with 50 Gy CIB irradiation could serve as a potential method to promote the accumulation of secondary metabolites in <em>B. chinense</em>. This finding provides strong support for the application of physical radiation technology to enhance the production of secondary metabolites in medicinal plants, offering new avenues for the cultivation of high-quality Chinese herbal medicines.</div></div>\",\"PeriodicalId\":34736,\"journal\":{\"name\":\"Plant Stress\",\"volume\":\"18 \",\"pages\":\"Article 101037\"},\"PeriodicalIF\":6.8000,\"publicationDate\":\"2025-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Stress\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667064X25003057\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Stress","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667064X25003057","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Carbon ion beam-induced radiation hormesis in Bupleurum chinense DC.: Insights from growth, physiological, and metabolomic analyses for increased bioactive substances
Bupleurum chinense DC (B. chinense) is an important medicinal plant widely used in Asian countries. However, the efficacy of its medicinal components has medicinal components during the domestication process from wild to cultivated lines. Studies have shown that appropriate stress, such as ionizing radiation, can promote the accumulation of metabolites in medicinal plants. Nevertheless, the effects of ionizing radiation on B. chinense remains unclear. In this study, we systematically investigated the stimulatory effect of carbon ion beams (CIB) pretreatment on the growth, physiology, and accumulation of secondary metabolites in B. chinense. Although CIB irradiation inhibited the seed germination and survival rates, by the age of 4 months, the plant height and leaf area of the irradiated group had recovered to the levels comparable to the control. The enhanced growth performance during later developmental stages may be attributed to radiation hormesis and ROS-mediated regulation in antioxidant system and the photosynthetic system. One and two years after irradiation at doses of 50 and 100 Gy, no significant differences in root biomass were observed compared to the control group. Metabolically, the content of total flavonoid, saikosaponin a and saikosaponin c were significantly increased following 50 Gy irradiation. Further metabolomic analysis revealed that intermediate metabolites in the flavonoid and terpenoid biosynthetic pathways were significantly up-regulated in the 50 Gy irradiation group. Compounds with pharmacological activity also accumulated in large quantities after irradiation. These results suggest that pretreatment with 50 Gy CIB irradiation could serve as a potential method to promote the accumulation of secondary metabolites in B. chinense. This finding provides strong support for the application of physical radiation technology to enhance the production of secondary metabolites in medicinal plants, offering new avenues for the cultivation of high-quality Chinese herbal medicines.
期刊介绍:
The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues.
Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and:
Lack of water (drought) and excess (flooding),
Salinity stress,
Elevated temperature and/or low temperature (chilling and freezing),
Hypoxia and/or anoxia,
Mineral nutrient excess and/or deficiency,
Heavy metals and/or metalloids,
Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection,
Viral, phytoplasma, bacterial and fungal plant-pathogen interactions.
The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.