Jiao Zhu , Liuyan Yang , Youming Cai , Xinhua Zeng , Yongchun Zhang , Weichang Huang
{"title":"适宜的光照强度通过调节淀粉和蔗糖代谢来促进白芨假球茎中多糖的合成","authors":"Jiao Zhu , Liuyan Yang , Youming Cai , Xinhua Zeng , Yongchun Zhang , Weichang Huang","doi":"10.1016/j.stress.2025.101041","DOIUrl":null,"url":null,"abstract":"<div><div><em>Bletilla striata</em> is a famous medicinal plant, which medicinal function on polysaccharide. However, the effect of light intensity on polysaccharide accumulation in <em>B. striata</em> pseudobulbs is largely unknown. <em>B. striata</em> plants were exposed to three different light intensities: low light (5–20 μmol·<em>m</em><sup>−2</sup>·s<sup>−1</sup>; LP), middle light (200 μmol·<em>m</em><sup>−2</sup>·s<sup>−1</sup>; MP), and high light (400 μmol·<em>m</em><sup>−2</sup>·s<sup>−1</sup>; HP). The results indicated that polysaccharide content of new pseudobulbs significantly increased under MP, and enrichment in starch and sucrose metabolism with metabolomics and transcriptomics. More sucrose was produced in the leaves through photosynthesis and was efficiently distributed to the pseudobulbs, where it was hydrolyzed into key metabolites for polysaccharide biosynthesis function by <em>SUS</em> and <em>CSLA</em> genes. The key metabolites in the polysaccharide biosynthetic pathway, such as sucrose-6-phosphate, fructose, fructose-6-phosphate, glucose-6-phosphate, GDP-mannose, and uridine 5′-diphospho-<span>d</span>-glucose, were upregulated under MP. Furthermore, the expression of the <em>UGP2</em> gene involved in polysaccharide biosynthesis was significantly higher under MP than under LP and HP. Conversely, sucrose in leaves was minimally transported into pseudobulbs due to insufficient photosynthesis under LP and energy metabolism related to carbohydrate degradation and oxidation was hindered under HP. Thus, suitable light intensity effectively stimulated polysaccharide formation in <em>B. striata</em> pseudobulbs through starch and sucrose metabolism–mediated regulation.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101041"},"PeriodicalIF":6.8000,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Suitable light intensity stimulated polysaccharide biosynthesis in bletilla striata pseudobulbs through regulating starch and sucrose metabolism\",\"authors\":\"Jiao Zhu , Liuyan Yang , Youming Cai , Xinhua Zeng , Yongchun Zhang , Weichang Huang\",\"doi\":\"10.1016/j.stress.2025.101041\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Bletilla striata</em> is a famous medicinal plant, which medicinal function on polysaccharide. However, the effect of light intensity on polysaccharide accumulation in <em>B. striata</em> pseudobulbs is largely unknown. <em>B. striata</em> plants were exposed to three different light intensities: low light (5–20 μmol·<em>m</em><sup>−2</sup>·s<sup>−1</sup>; LP), middle light (200 μmol·<em>m</em><sup>−2</sup>·s<sup>−1</sup>; MP), and high light (400 μmol·<em>m</em><sup>−2</sup>·s<sup>−1</sup>; HP). The results indicated that polysaccharide content of new pseudobulbs significantly increased under MP, and enrichment in starch and sucrose metabolism with metabolomics and transcriptomics. More sucrose was produced in the leaves through photosynthesis and was efficiently distributed to the pseudobulbs, where it was hydrolyzed into key metabolites for polysaccharide biosynthesis function by <em>SUS</em> and <em>CSLA</em> genes. The key metabolites in the polysaccharide biosynthetic pathway, such as sucrose-6-phosphate, fructose, fructose-6-phosphate, glucose-6-phosphate, GDP-mannose, and uridine 5′-diphospho-<span>d</span>-glucose, were upregulated under MP. Furthermore, the expression of the <em>UGP2</em> gene involved in polysaccharide biosynthesis was significantly higher under MP than under LP and HP. Conversely, sucrose in leaves was minimally transported into pseudobulbs due to insufficient photosynthesis under LP and energy metabolism related to carbohydrate degradation and oxidation was hindered under HP. Thus, suitable light intensity effectively stimulated polysaccharide formation in <em>B. striata</em> pseudobulbs through starch and sucrose metabolism–mediated regulation.</div></div>\",\"PeriodicalId\":34736,\"journal\":{\"name\":\"Plant Stress\",\"volume\":\"18 \",\"pages\":\"Article 101041\"},\"PeriodicalIF\":6.8000,\"publicationDate\":\"2025-09-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Stress\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667064X25003094\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Stress","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667064X25003094","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Suitable light intensity stimulated polysaccharide biosynthesis in bletilla striata pseudobulbs through regulating starch and sucrose metabolism
Bletilla striata is a famous medicinal plant, which medicinal function on polysaccharide. However, the effect of light intensity on polysaccharide accumulation in B. striata pseudobulbs is largely unknown. B. striata plants were exposed to three different light intensities: low light (5–20 μmol·m−2·s−1; LP), middle light (200 μmol·m−2·s−1; MP), and high light (400 μmol·m−2·s−1; HP). The results indicated that polysaccharide content of new pseudobulbs significantly increased under MP, and enrichment in starch and sucrose metabolism with metabolomics and transcriptomics. More sucrose was produced in the leaves through photosynthesis and was efficiently distributed to the pseudobulbs, where it was hydrolyzed into key metabolites for polysaccharide biosynthesis function by SUS and CSLA genes. The key metabolites in the polysaccharide biosynthetic pathway, such as sucrose-6-phosphate, fructose, fructose-6-phosphate, glucose-6-phosphate, GDP-mannose, and uridine 5′-diphospho-d-glucose, were upregulated under MP. Furthermore, the expression of the UGP2 gene involved in polysaccharide biosynthesis was significantly higher under MP than under LP and HP. Conversely, sucrose in leaves was minimally transported into pseudobulbs due to insufficient photosynthesis under LP and energy metabolism related to carbohydrate degradation and oxidation was hindered under HP. Thus, suitable light intensity effectively stimulated polysaccharide formation in B. striata pseudobulbs through starch and sucrose metabolism–mediated regulation.
期刊介绍:
The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues.
Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and:
Lack of water (drought) and excess (flooding),
Salinity stress,
Elevated temperature and/or low temperature (chilling and freezing),
Hypoxia and/or anoxia,
Mineral nutrient excess and/or deficiency,
Heavy metals and/or metalloids,
Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection,
Viral, phytoplasma, bacterial and fungal plant-pathogen interactions.
The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.