Plant Stress最新文献

{"title":"Comprehensive analysis of Vicia faba light-harvesting chlorophyll a/b binding protein (Lhc) revealed the roles of VfLhcb1.5, VfLhcb3.3, VfLhcb4, and VfLhca4 in photosynthesis and stress tolerance","authors":"Ting Huang ,&nbsp;Limeng Dong ,&nbsp;Shuo Han ,&nbsp;Xiaowen Han ,&nbsp;Junliang Yin ,&nbsp;Lu Hou ,&nbsp;Yujiao Liu","doi":"10.1016/j.stress.2025.101052","DOIUrl":"10.1016/j.stress.2025.101052","url":null,"abstract":"<div><div>In addition to its indispensable role in photosynthesis, <u>L</u>ight-<u>h</u>arvesting <u>c</u>hlorophyll a/b binding protein (Lhc) is also involved in plant growth, development, and stress responses. However, the specific roles of <em>Vicia faba</em> VfLhcs in photosynthesis and stress tolerance remain unclear. Here, <em>in silico</em> analysis and <em>in vivo</em> assays were conducted to investigate the characteristics and functions of VfLhcs. Phylogenetic analysis grouped 23 <em>VfLhcs</em> into three subfamilies. Their promoter regions were enriched with cis-elements responsive to light, plant hormones, abiotic stress, and plant growth and development. RT-qPCR analysis revealed that <em>VfLhcs</em> were highly expressed in chlorophyll-containing tissues and exhibited stress-specific regulation. Drought stress (20 % PEG-6000, 72 h) induced a 4.9- to 50.1-fold upregulation in roots, whereas salt stress (200 mM NaCl, 72 h) and darkness (0 h light/24 h dark, 72 h) reduced expression by 93.0–99.3 % and 78.2–100.0 %, respectively. Confocal microscopy confirmed that VfLhcs were localized in chloroplasts. Overexpression of <em>VfLhcb1.5, VfLhcb3.3</em>, and <em>VfLhcb4</em> increased chlorophyll content by 20.0 -35.7 %, which in turn enhanced the net photosynthetic rate by 13.5–23.1 %. Under various stress conditions, <em>VfLhcb1.5, VfLhcb4</em>, and <em>VfLhca4</em> promoted significant over-accumulation of reactive oxygen species (ROS), disrupting ROS homeostasis. While this enhanced response improved tolerance to abiotic stresses, it paradoxically facilitated infection by the pathogen <em>Phytophthora infestans</em>, suggesting that these genes may act as susceptibility factors under biotic stress. In conclusion, this comprehensive analysis of <em>VfLhcs</em> highlights their roles in photosynthesis and responses to both abiotic and biotic stresses, providing a foundation for further exploration of their functional mechanisms.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101052"},"PeriodicalIF":6.8,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145157664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering heat-enhanced resistance of grapevine berries to Botrytis cinerea highlights differential cuticular and secondary metabolite accumulations between Merlot and Cabernet Sauvignon","authors":"Erwan Chavonet ,&nbsp;Cathleen Mirande-Ney ,&nbsp;Sarah Bernardo ,&nbsp;Clément Guinand ,&nbsp;Ghislain Delestre ,&nbsp;Xi Zhan ,&nbsp;Josep Valls Fonayet ,&nbsp;Sylvain Prigent ,&nbsp;Pierre Van Delft ,&nbsp;Stéphanie Pascal ,&nbsp;Jérôme Joubès ,&nbsp;David Lecourieux ,&nbsp;Marc Fermaud ,&nbsp;Frédéric Domergue","doi":"10.1016/j.stress.2025.101051","DOIUrl":"10.1016/j.stress.2025.101051","url":null,"abstract":"<div><div>In the context of climate change, temperature is a key abiotic driver of bunch microclimate, which, in order to reduce <em>Botrytis cinerea</em> development, is often managed in vineyards <em>via</em> practices such as leaf removal. The heat-dependent mechanisms of pathogen resistance in grapevines nevertheless remain to be fully elucidated. In this study, the effect of heat stress (HS) applied specifically to green bunches on infections caused by <em>B. cinerea</em> on ripe berries inoculated 23 days later was assessed for two years in a greenhouse. Bunches of the Cabernet Sauvignon (CS) and Merlot (M) cultivars were heated 6 days 8 h daily with a 10 °C increased temperature. <em>In vitro</em> bio tests highlighted a significant heat-enhanced resistance only in CS berries, whereas a stable constitutive resistance characterized the M berries. Bunch veraison and total sugar content were not affected by HS, rejecting its effect on maturation dynamics. Therefore, berry preformed barriers at the time of inoculation, which can hinder fungal colonization, were investigated. While HS had nearly no effect on waxes, it significantly affected the cutin content in both varieties, and more significantly its composition in CS. Similarly, the antifungal skin condensed tannins overaccumulated following HS in both cultivars, and their basal level was greater in CS than in M. Otherwise, M accumulated more stilbene and flavonoid compounds, which may have contributed to the observed varietal resistance. Finally, untargeted metabolomic data revealed a range of compounds modulated by HS in CS as potential candidates involved in resistance.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101051"},"PeriodicalIF":6.8,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethylene–potassium interactions safeguard ionic balance, chlorophyll biosynthesis, and source–sink metabolism to sustain wheat growth and yield responses under salt stress","authors":"Sayeda Khatoon,&nbsp;Moksh Mahajan,&nbsp;M.Iqbal R. Khan","doi":"10.1016/j.stress.2025.101049","DOIUrl":"10.1016/j.stress.2025.101049","url":null,"abstract":"<div><div>Significant reduction in crop yields has been caused due to salt-impacted agricultural soils. In order to meet hunger requirements, significant solutions are required for speeding up agricultural production even in salt-impacted soils. As salt stress reduces the uptake of potassium (K⁺), replenishing the K⁺ nutrition may serve as a tolerance mechanism. The present study investigated the different K⁺ levels (low, optimum, and excess) along with a stress hormone, ethylene in modulating plant responses under salt stress. The results demonstrated that the interplay of ethylene and K⁺ significantly altered salt induced inhibition through improved K⁺retention, chlorophyll biosynthesis, and source-sink metabolism to protect growth and yield traits. Additionally, ethylene and K⁺ improved key pathways such as defense machinery, nitric oxide (NO) biosynthesis along with secondary metabolites (lignin, cellulose, flavonoid and phenol) to overcome salt-induced adversities. The impacts posed by the interplay between ethylene and different K⁺ levels depicted a variable mitigation of salt induced adversities. Conclusively, ethylene and optimum K⁺ treatment best excruciated the salt-inhibited responses. Supplementation of norbonadiene (NBD, ethylene action inhibitor) further confirmed the role of interplay between ethylene and differential K⁺ concentrations in mediating the salt stress responses. The present study pose as a significant strategy towards cultivar development programs to counter salt-inhibited constraints and paves way to ensure crop security.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101049"},"PeriodicalIF":6.8,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interaction between BnaAIF1 and BnaICE1 enhances the low-temperature tolerance of Brassica napus","authors":"Xiaoyan Xia ,&nbsp;Shun Li ,&nbsp;Lei Sun,&nbsp;Zhonghua Wang,&nbsp;Xiaoyu Chen,&nbsp;Bo Yang,&nbsp;Zixuan Zhou,&nbsp;Xin He","doi":"10.1016/j.stress.2025.101053","DOIUrl":"10.1016/j.stress.2025.101053","url":null,"abstract":"<div><div>Rapeseed (<em>Brassica napus</em> L.) is an important oilseed crop in the world, it’s often damaged by low-temperature (especially freezing) stress, which has the potential to cause significant yield losses. However, little is known about the molecular mechanisms for coping with low-temperature stress in rapeseed. In this study, a total of 24 atypical bHLH transcription factor <em>AIF</em> (ATBS1 INTERACTING FACTOR) gene family members were identified by systematically bioinformatics analysis in rapeseed, among which all six <em>BnaAIF1</em> genes were strongly induced by low temperature stress, especially <em>BnaAIF1-A03/C03</em>. Overexpression of <em>BnaAIF1-C03</em> improved the seed germination under cold stress (4 °C) and the survival rate of seedlings under freezing stress (-4 °C) in rapeseed, with enhanced stability of light system II (Fv/Fm and NPQ_Lss increased) and reduced oxidative damage (decreased contents of H₂O₂ and MDA). Further analysis revealed that BnaAIF1-C03 was localized in the nucleus and interacted with BnaICE1, activating the ICE1-CBF-COR pathway genes and thus participating in the low-temperature stress response of rapeseed. Overall, this study provides an important theoretical basis and new target gene for future breeding of low-temperature tolerant rapeseed.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101053"},"PeriodicalIF":6.8,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145157790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaping plant resilience through nitrate nutrition: Insights from the crosstalk between nitrate signaling and stress response","authors":"Jingjing Mao,&nbsp;Jinhao Sun,&nbsp;Zhen Tian,&nbsp;Duanfei Wang,&nbsp;Yating Yu,&nbsp;Shaopeng Li","doi":"10.1016/j.stress.2025.101047","DOIUrl":"10.1016/j.stress.2025.101047","url":null,"abstract":"<div><div>Environmental stresses are a constant challenge for plants and a key determinant of how plants respond to these stressors is their nutritional status, particularly the availability of essential nutrients such as nitrate (NO₃^-). Recent research has uncovered a complex crosstalk between nitrate signaling pathways and stress response. In addition to being a key macronutrient, NO₃^- acts as a signaling molecule that modulates various aspects of plant metabolism and defense. This review explores two layers of interaction between nitrate signaling and plant resilience: indirect crosstalk mediated by the regulation of interconnected physiological processes, and direct crosstalk via specific regulatory proteins. By examining how NO₃^- availability influences stress responses, we aim to provide new insights into the mechanisms underlying plant resilience. This understanding holds significant potential for promoting more sustainable agricultural practices and enhancing crop performance under stress conditions.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101047"},"PeriodicalIF":6.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abiotic stress tolerance and root nodule-associated bacteria in Lablab purpureus","authors":"Adrián Sapiña-Solano ,&nbsp;Alberto Yago ,&nbsp;Claudia Pallotti ,&nbsp;Pablo G. Acosta-Quezada ,&nbsp;Monica Boscaiu ,&nbsp;Ana Fita ,&nbsp;Oscar Vicente ,&nbsp;Mario X. Ruiz-González","doi":"10.1016/j.stress.2025.101043","DOIUrl":"10.1016/j.stress.2025.101043","url":null,"abstract":"<div><div>Climate change effects pose significant challenges to 21st-century society. Abiotic stresses such as salinity and drought represent a major risk to biodiversity and threaten crops, leading to economic losses and food security concerns. Promoting sustainable agriculture is both a solution and a necessity to address these challenges, requiring the assessment of stress-tolerant crops and the identification of beneficial microorganisms to enhance resilience, soil health, and productivity while reducing agrochemical reliance. In this study, we assessed the tolerance of <em>Lablab purpureus</em>, an underutilised non-European legume with high nutraceutical potential, to saline and water stress. Nodule-associated bacteria were isolated from two Mediterranean ecosystems, and three salt-tolerant strains (CJND1, LN1RA and LN3BA) were selected to evaluate their interaction with the alien crop, <em>L. purpureus</em>, and their effects on plant abiotic stress tolerance and traits. We analysed phenological, biomass, and root traits, photosynthetic pigments, osmolytes, ion contents (Na⁺, Cl⁻, K⁺, Ca²⁺), oxidative stress markers, antioxidant compounds, and antioxidant enzyme activities. The bacteria produced heterogeneous effects. CJND1 and LN3BA promoted root length, whereas LN3BA also increased root surface area. LN1RA increased the Cha/Chb ratio and enhanced nodulation under water stress. Overall, nodule-associated bacteria positively influenced root traits. Salinity reduced biomass, increased proline levels, and led to Na⁺ and Ca²⁺ accumulation in roots while blocking Na⁺ translocation to aerial parts, whereas water stress activated glutathione reductase. . Our results suggest that <em>L. purpureus</em> is a promising crop for mild salt stress in the Mediterranean and reveal the heterogeneity of the plant and root nodule bacteria interactions, highlighting their agronomic potential.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101043"},"PeriodicalIF":6.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spaceflight Disrupts Transcriptome Dynamics and Germination in Date Palm (Phoenix dactylifera) Seeds","authors":"Shafeeq Rahman ,&nbsp;Shamma Aldhaheri ,&nbsp;Wesam Khader ,&nbsp;Jeffin Rockey ,&nbsp;Khaled Masmoudi ,&nbsp;Nadia Hassan Tawfiq ,&nbsp;Abdul Jaleel Cheruth ,&nbsp;Wasef Al-Zayadneh ,&nbsp;Sonu Krishankumar ,&nbsp;Hoor Al Mazmi ,&nbsp;Hamda Faisal Al Shehhi ,&nbsp;Rashid Abdulla Alzaabi ,&nbsp;Shyam Sreedhara Kurup","doi":"10.1016/j.stress.2025.101045","DOIUrl":"10.1016/j.stress.2025.101045","url":null,"abstract":"<div><div>Spaceflight offers a unique setting for investigating plant stress responses and molecular pathways that enable adaptation to microgravity. Here, we examined the physiological and transcriptomic responses of date palm (<em>Phoenix dactylifera</em> L.) seeds maintained aboard the International Space Station (ISS) for six months. Germination assays revealed that space-exposed seeds from six cultivars failed to germinate, even after exogenous gibberellic acid and cytokinin treatments, whereas ground controls achieved 100% germination. Biochemical analyses indicated significant reductions in protein content, α-amylase activity, and proline levels, indicating impaired antioxidant capacity and reduced reserve mobilisation. Transcriptomic profiling of three representative cultivars (Lulu, Majdool, and Meselli) revealed extensive differential expression with Majdool exhibiting the strongest suppression. Downregulated genes were enriched in mitochondrial respiration, antioxidant defense, and auxin and ethylene signalling, whereas abscisic acid-related dormancy genes were upregulated. Additional disruptions included the suppression of DNA repair mechanisms, cytoskeletal organisation, and negative gravitropism regulators. The qRT-PCR validation of the selected genes confirmed these trends. Together, these results indicate that spaceflight seeds remain viable but enter a state of physiological stasis enforced by ABA dominance, impaired energy metabolism, oxidative imbalance, and disrupted growth orientation. To our knowledge, this is the first report on the long-term space exposure of a perennial fruit tree, providing new insights into seed biology under microgravity and highlighting critical challenges for future extraterrestrial agriculture.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101045"},"PeriodicalIF":6.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of phenology and agronomical performance of Syrah grafted on two rootstocks under combined salinity and water stress conditions: A three-year field study","authors":"Kidanemaryam Reta ,&nbsp;Yaniv Lupo ,&nbsp;Noga Sikron Persi ,&nbsp;Naftali Lazarovitch ,&nbsp;Aaron Fait","doi":"10.1016/j.stress.2025.101050","DOIUrl":"10.1016/j.stress.2025.101050","url":null,"abstract":"<div><div>Climate change is challenging global viticulture through increasing drought and salinization, making rootstock selection critical. However, field-based understanding of rootstock-mediated stress responses remains limited. Here, we examined the mediation of Sélection Oppenheim 4 (SO4) and 1103 Paulsen (PL1103) rootstocks on Syrah grapevines' responses under combined water and salinity stress over 3 years (2022‒2024). We observed that the soil electrical conductivity (EC_e1:1) increased from 2.09 to 8 dS <em>m</em>⁻¹ under severe stress, while soil chloride concentration ([Cl⁻]) reached 716.5 mg <em>L</em>⁻¹ in Syrah grafted onto PL1103 (SY_PL1103) and 954.9 mg <em>L</em>⁻¹ in Syrah grafted onto SO4 (SY_SO4), compared with 90‒162 mg L^‒1 under the control conditions coupled with varied leaf [Cl⁻] between grafts. Salinity delayed flowering time by 7‒11 days when the EC_e1:1 exceeded 2.79 dS m^‒1 in SY_PL1103 and 1.99 dS m^‒1 in SY_SO4. Nevertheless, SY_SO4 maintained greater physiological performance with 56.8 % greater photosystem II efficiency, 48.71 % greater electron transport rate, and higher non-photochemical quenching across conditions than SY_PL1103, effectively managing excess light as heat to prevent photodamage. SY_SO4 vines exhibited a significantly lower yield reduction (36.3 % vs 56.4 % in SY_PL1103) and better salinity tolerance, with yield decreasing by only 2.63 t ha⁻¹ per unit increase in EC_e1:1 above the threshold (SY_PL1103 = 3.47 dS <em>m</em>⁻¹ and SY_SO4 = 2.71 dS <em>m</em>⁻¹) compared with 7.78 t ha⁻¹ in SY_PL1103. SY_SO4 showed smaller photosynthetic and morphological changes to combined stressors, with higher soil and lower leaf [Cl−] indicating better ion exclusion, offering valuable insights and practical solutions for resilient grapevine breeding and vineyard management strategies in saline-prone arid regions.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101050"},"PeriodicalIF":6.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated physiological, transcriptomic, and metabolomic analyses reveal the response mechanism of the CsNADP-ME4 gene to drought stress in cucumber (Cucumis sativus L.)","authors":"Putao Wang ,&nbsp;Jiali Lin ,&nbsp;Zijin Xiang ,&nbsp;Xia Zou ,&nbsp;Sha Luo ,&nbsp;Yao Xiao ,&nbsp;Jingyu Sun ,&nbsp;Shenglin Wang ,&nbsp;Qianglong Zhu ,&nbsp;Cuixiang Huang ,&nbsp;Qinghong Zhou ,&nbsp;Nan Shan","doi":"10.1016/j.stress.2025.101048","DOIUrl":"10.1016/j.stress.2025.101048","url":null,"abstract":"<div><div>Drought is a pervasive abiotic stress that poses a significant threat to global vegetable production. Although the involvement of NADP-malic enzyme (NADP-ME) in plant drought resistance is documented, its specific function and regulatory mechanisms in cucumber remain underexplored. In this study, the NADP-ME gene <em>CsNADP-ME4</em> was predominantly expressed in cucumber leaves and was inducible by drought stress. Subcellular localization confirmed the chloroplast targeting of CsNADP-ME4. Functional analysis using RNA interference (RNAi) indicated that downregulating <em>CsNADP-ME4</em> increased drought sensitivity, as evidenced by severe leaf wilting, reduced photosynthetic efficiency, and altered activities of osmotic regulators and protective enzymes compared to wild-type plants. Integrated transcriptomic and metabolomic analyses revealed that <em>CsNADP-ME4</em> silencing disrupts the levels of sugars, amino acids, and phytohormones, alongside extensive changes in gene expression. Promoter analysis identified MYB-binding <em>cis</em>-elements, and subsequent experiments, including yeast one-hybrid, dual luciferase reporter, and electrophoretic mobility shift assays, confirmed that the transcription factor CsMYB16 directly binds to the <em>CsNADP-ME4</em> promoter to activate its transcription. In conclusion, these results emphasize the critical role of <em>CsNADP-ME4</em> in enhancing drought resilience and suggests a broader function for MYB transcription factors in regulating stress-responsive genes. These findings provided precise targets and genetic resources for molecular design breeding of cucumbers, and offered a solid theoretical basis for the development of new cucumber varieties that are drought-tolerant.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101048"},"PeriodicalIF":6.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis","authors":"Emmanuel Flores-Hernández ,&nbsp;María Elisa Gonzalez ,&nbsp;Paulina Alvarado-Guitron ,&nbsp;Francisco I. Jasso-Robles ,&nbsp;Cesaré Ovando-Vázquez ,&nbsp;Juan Francisco Jiménez-Bremont ,&nbsp;Sanja Ćavar Zeljković ,&nbsp;Markéta Ulbrichová ,&nbsp;Nuria De Diego ,&nbsp;Margarita Rodríguez-Kessler","doi":"10.1016/j.stress.2025.101046","DOIUrl":"10.1016/j.stress.2025.101046","url":null,"abstract":"<div><div>The study of polyamine transport in plants has become increasingly important due to the central role of these amines in regulating growth, development, adaptation, and stress responses. This research focused on the <em>Arabidopsis thaliana Polyamine Uptake Transporters</em> gene family under conditions of systemic acquired resistance. We evaluated all single mutants of this gene family and found that the <em>put2-1</em> mutant abolished systemic acquired resistance while enhancing basal resistance to <em>Pseudomonas syringae</em> pv. <em>tomato</em> DC3000. In contrast, the <em>35S::PUT2</em> overexpression lines showed improved resistance and reduced bacterial titers compared to wild-type plants. RNA-seq analysis revealed that the <em>put2-1</em> mutant had deregulated expression of genes involved in the biosynthesis, signaling, and inactivation of salicylic acid and N-hydroxypipecolic acid. Most of these genes were transcriptionally upregulated by putrescine in wild-type plants, but not in the <em>put2-1</em> mutant. Putrescine supplementation increased endogenous putrescine and salicylic acid levels in wild-type plants but not in <em>put2-1</em>, highlighting the essential role of this transporter in facilitating putrescine mobilization and regulating salicylic acid in distal tissues. We found that the defective systemic acquired resistance phenotype in the <em>put2-1</em> mutant was linked to changes in the timing of polyamines, ROS, phenolic compound accumulation, and alterations in stomatal immunity. Our study emphasizes the key role of the Polyamine Uptake Transporter 2 (PUT2/LAT4) in establishing systemic acquired resistance in <em>Arabidopsis</em>, while also maintaining the plant’s intrinsic basal resistance mechanisms. These findings offer valuable insights into the complex mechanisms of plant resistance, positioning polyamine transport as a central hub in systemic responses.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 101046"},"PeriodicalIF":6.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}