高光强引起的细胞分裂素升高是水稻耐热性提高的重要原因

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2025-05-23 DOI:10.1016/j.stress.2025.100904

Sylva Prerostova , Jana Jarošová , Petre Dobrev , Alena Gaudinova , Vojtech Knirsch , Eva Kobzova , Kinga Benczúr , Gabriella Szalai , Ondrej Novak , Radomira Vankova

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引用次数: 0

摘要

光是影响植物发育的重要因素。研究了水稻（Oryza sativa）对强光（HL, 1300 μmol m−2 s−1,27°C）的响应。由于蒸腾流，HL显著提高了叶片的根生细胞分裂素和反式玉米素。尽管脱落酸（ABA）和茉莉酸（JA）水平仅发生轻微变化，但HL降低了ABA生物合成基因NCED5的表达，同时通过下调抑制因子JAZ9的表达促进了JA信号通路。光系统II的最大量子产率（Fv/Fm）下降，表明光系统II有保护作用。HL刺激糖的产生，尤其是葡萄糖和甘露糖。由于细胞分裂素水平的调节（应用外源细胞分裂素或其降解抑制剂）促进了水稻的耐热性，因此我们测试了HL处理引起细胞分裂素增加对高温反应的潜在积极影响。研究了热胁迫（HS, 40°C, 6 h）本身和联合HL对叶片、冠和根的影响。HS适用于整株（HS- wp），或针对叶片（HS- l）或根（HS- r）。HS处理降低了反式玉米素水平。在叶片上同时施用HL和HS （HL+HS- wp, HL+HS- l）可降低这一影响。HL联合HS对叶片也有刺激JA和生长素吲哚-3-乙酸的作用。hs标记基因HSP90.2、HSP90.3、HSP26.2和HSFA2d以及编码抗氧化酶（特别是铁超氧化物歧化酶）的基因表达较轻，表明HL提高了水稻的耐热性。HL降低了HS对根系糖的强烈负面影响。结果表明，HL中期处理对水稻耐热性有积极影响，揭示了一种刺激植物防御的新策略。

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Cytokinin elevation caused by high light intensity contributes substantially to the increase of thermotolerance of rice plants

Light is a crucial factor affecting plant development. Rice (Oryza sativa) responses to high light (HL, 1300 μmol m⁻² s⁻¹, 27 °C) were characterized. HL significantly elevated root-born cytokinin, trans-zeatin in leaves due to transpiration stream. Despite only minor changes in abscisic acid (ABA) and jasmonic acid (JA) levels, HL decreased expression of ABA biosynthetic gene NCED5, while promoted JA signalling pathway by down-regulated expression of repressor JAZ9. The maximum quantum yield of photosystem II (F_v/F_m) decreased, suggesting protection of photosystem II. HL stimulated sugar production, especially glucose and mannose. As modulation of cytokinin levels (applying exogenous cytokinin or inhibitors of their degradation) promoted rice thermotolerance, HL treatment causing cytokinin increase was tested on its potential positive effect on response to elevated temperature.

The effects of heat stress (HS, 40 °C for 6 h) per se and in combination with HL were evaluated in leaves, crowns and roots. HS was applied to whole plants (HS-WP), or targeted to leaves (HS-L) or roots (HS-R). HS treatments down-regulated trans-zeatin levels. Simultaneous application of HL and HS to leaves (HL+HS-WP, HL+HS-L) reduced this impact. HL combined with HS affecting leaves also stimulated JA and auxin indole-3-acetic acid. Milder expression of HS-marker genes HSP90.2, HSP90.3, HSP26.2 and HSFA2d, and genes coding antioxidant enzymes (especially Fe-superoxide dismutase) indicated that HL increased rice thermotolerance. HL minimized strong negative effect of HS on sugars in roots. The data showed positive impacts of mid-term HL treatment on thermotolerance of rice, revealing a novel strategy for stimulation of plant defence.

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.