Plant Stress最新文献

{"title":"Increasing salinity sequentially induces salt tolerance responses in Szarvasi-1 energy grass","authors":"Vitor Arcoverde Cerveira Sterner ,&nbsp;Kristóf Jobbágy ,&nbsp;Brigitta Tóth ,&nbsp;Szabolcs Rudnóy ,&nbsp;Gyula Sipos ,&nbsp;Ferenc Fodor","doi":"10.1016/j.stress.2024.100572","DOIUrl":"10.1016/j.stress.2024.100572","url":null,"abstract":"<div><p>Soil salinity causes severe physiological disorders, decline in biomass, and crop production worldwide becoming more critical with global climate change. Consequently, salt-tolerant varieties received major focus in all sectors of agriculture. Biomass plants such as Szarvasi-1 energy grass (<em>Elymus elongatus</em> subsp. <em>ponticus</em> cv. Szarvasi-1) may play an important role in energy production if they are tolerant to environmental stresses. In this study, Szarvasi-1 energy grass has been investigated to reveal its tolerance to 50–200 mM NaCl in hydroponics. Significant decline in stomatal conductance appeared at 100 mM NaCl treatment but fresh and dry weight and the maximal quantum efficiency of PSII decreased only at 200 mM NaCl. Relative water content and total chlorophyll concentration did not change compared to the control. Leaf water potential was maintained at the control level for one week NaCl exposure, decrease became significant only after two weeks. Malondialdehyde concentration did not refer to oxidative stress. In the element composition of the plants, remarkable increase was found only for Mo whereas Ca, K, S, P, Mn decreased compared to the control. K to Na ratio remained higher than one in the shoot even at 200 mM NaCl. Salt treatment caused temporal and concentration-dependent changes in the expression of genes in the phenylpropanoid pathway, Na transport, photosynthesis, and cellular protection and repair. Szarvasi-1 was found to be fairly tolerant to NaCl which induced a sequential response switching on vacuolar compartmentalization at 50 mM, Na efflux at 100 mM, and cellular protection and repair at 200 mM.</p></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100572"},"PeriodicalIF":6.8,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002252/pdfft?md5=5c4305b140e57e1217a90f0739995e1f&pid=1-s2.0-S2667064X24002252-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142049597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of plant defenses by Jasmonic acid and salicylic acid in Capsicum annuum L. against Fusarium wilt in response to Fusarium oxysporum pathogen","authors":"Gousia Majeed ,&nbsp;Wasifa Noor ,&nbsp;Rafiq Lone ,&nbsp;Smita Agrawal ,&nbsp;Abdullah Alaklabi ,&nbsp;Manzoor Ahmad Shah ,&nbsp;Azra N. Kamili","doi":"10.1016/j.stress.2024.100571","DOIUrl":"10.1016/j.stress.2024.100571","url":null,"abstract":"<div><p>Wilt disease in chilli caused by <em>Fusarium oxysporum</em> is a serious and global issue in chilli production, necessitating greater knowledge of plant defense systems for overall disease protection and management. In an eco-friendly integrated agricultural protection system, the introduction of chemical elicitors like jasmonic acid (JA) and salicylic acid (SA) represents an attractive new prospect means of controlling bacterial and fungal diseases. In the present study effectiveness of JA and SA as elicitors in disease progression was investigated in <em>Capsicum annuum</em> L. c.v., Kashmiri longum (chilli) plants against Fusarium wilt caused by pathogen <em>Fusarium oxysporum</em>. The experiment had eight sets of plants as control (untreated, uninfected), infected (untreated, infected), and one set of plants for each concentration of JA treatment (50 µM, 100 µM, 150 µM) + pathogen, and SA treatment (50 µM, 100 µM, 150 µM) + pathogen. Pre-treatment of JA and SA elicitors to <em>F.oxysorum-</em>infected chilli plants showed significant decrease in disease severity index as well as in disease symptoms compared to pathogen-infected and control plants. There was a substantial increase in the activities of antioxidant and defense enzymes in elicitor treated samples post pathogen inoculation. Exogenous application of JA and SA enhanced the growth of morphological parameters (shoot length, root length, number of leaves, leaf area, and plant height). Moreover, the statistical analysis using two-way ANOVA and Tukey test revealed significant differences between treatments, and days post infection in comparison to control. The study found that each elicitor treatment significantly differs in the type of enzymes activated and their amounts post-inoculation, indicating distinct host reaction patterns. Overall, the current study highlights the induced defense resistance role of JA and SA in chilli plants in response to <em>F.oxysporum</em> and JA was found most effective concerning disease protection followed by SA.</p></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100571"},"PeriodicalIF":6.8,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002240/pdfft?md5=1a5d09acd0ec71c9fce73eeaaae83066&pid=1-s2.0-S2667064X24002240-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142137146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bread wheat cultivar Popo harbors QTLs for seedling and adult plant resistance to leaf rust in South African and Argentine environments","authors":"Sandiswa Figlan ,&nbsp;Tsepiso Hlongoane ,&nbsp;Carlos Bainotti ,&nbsp;Pablo Campos ,&nbsp;Leonardo Vanzetti ,&nbsp;Gabriela Edith Tranquilli ,&nbsp;Toi John Tsilo","doi":"10.1016/j.stress.2024.100570","DOIUrl":"10.1016/j.stress.2024.100570","url":null,"abstract":"<div><p>Leaf rust, caused by the fungus <em>Puccinia triticina</em> Eriks (<em>Pt</em>), is a destructive disease affecting wheat (<em>Triticum aestivum</em> L.) production in many countries, and a serious threat to food security. As a result, several breeding programs have included leaf rust resistance as an important trait. The discovery and identification of new resistance genes that could aid in incorporating durable or long-lasting leaf rust resistance into wheat is fundamental in these breeding programs. The present study aimed to identify quantitative trait loci (QTLs) for leaf rust resistance in 127 recombinant inbred lines (RIL) developed from the cross between the resistant wheat cultivar Popo and the susceptible cultivar Kariega. The RIL population and parental lines were phenotyped for leaf rust infection type and severity at seedling and adult plant stage, respectively. The former in the greenhouse (in Argentina) and the latter in multiple field test environments comprising 3 locations in South Africa (in Tygerhoek in the Western Cape Province during the 2014, 2015, 2017 and 2018 cropping seasons; Clarens during 2014, 2016 and 2017 cropping seasons and in Bethlehem in the Free State Province during 2017 cropping season) and in 1 location in Argentina (during the 2017 and 2018 cropping seasons in Marcos Juárez, Córdoba Province). The population was genotyped using genotyping-by-sequencing. A total of 12,080 silicoDArT and 2,669 SNP markers were used for QTL analysis. In total, 25 putative QTLs for resistance to leaf rust at seedling and adult plant stages were identified, including 5 QTLs for seedling and 20 QTLs for adult plant resistance (APR). Interestingly, both Popo and Kariega contributed with alleles for resistance. Significant loci for reducing leaf rust infection at seedling stage were designated <em>QLr.arc-1A, QLr.arc-2B, QLr.arc-5B, QLr.arc-6A</em> and <em>QLr.arc-6D</em>. Three minor QTLs derived from Popo designated as <em>QLr.arc-1B1, QLr.arc-2D</em> and <em>QLr.arc-3D</em> were also detected from the field tests, explaining 5–10%, 10–16% and 5–7% of the phenotypic variance, respectively. The identified QTLs and their closely linked silicoDArT and SNP-based markers can be used for fine mapping and candidate gene discovery in wheat breeding programs targeting durable leaf rust resistance.</p></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100570"},"PeriodicalIF":6.8,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002239/pdfft?md5=e41d7d7088a0529dfe81d93d54f846fc&pid=1-s2.0-S2667064X24002239-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142058407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing plant-mediated RNAi for effective management of Phthorimaea absoluta by targeting AChE1 and SEC23 genes","authors":"Muneeb Hassan Hashmi ,&nbsp;Haneef Tariq ,&nbsp;Faisal Saeed ,&nbsp;Ufuk Demirel ,&nbsp;Ayhan Gökçe ,&nbsp;Hans Merzendorfer ,&nbsp;Emre Aksoy ,&nbsp;Allah Bakhsh","doi":"10.1016/j.stress.2024.100569","DOIUrl":"10.1016/j.stress.2024.100569","url":null,"abstract":"<div><p>Tomato production on a global scale is under persistent pressure due to the devastating impact of <em>Phthorimaea absoluta</em> Meyrick (Lepidoptera: Gelechiidae), the South American tomato leaf miner. To combat this devastating pest, we explored the potential of plant-mediated RNA interference (RNAi) as a novel strategy for its management. Using transgenic techniques, we developed RNAi constructs (<em>p35S::dsAChE1, p35S::dsSEC23</em>) targeting crucial genes, <em>AChE1</em> and <em>SEC23</em>, in <em>P. absoluta</em>. These genes play pivotal roles in insect physiology and development. The transformation of tomato cultivar Rio Grande was carried out with these RNAi constructs using <em>Agrobacterium tumefaciens</em>. The results demonstrated a significant reduction in transcript levels of both <em>AChE1</em> and <em>SEC23</em> in <em>P. absoluta</em>. Silencing <em>AChE1</em> resulted in substantial mortality rates, reduced larval weight gain, and deformities, highlighting its pivotal role in insect survival. <em>SEC23</em> gene silencing also induced mortality and influenced insect physiology. Furthermore, we explored the susceptibility of <em>AChE1</em> to organophosphate insecticides, revealing its relevance in insecticide susceptibility. These findings support the potential of <em>AChE1</em> and <em>SEC23</em> as valuable targets for RNAi-based control of <em>P. absoluta</em> for the first time, providing multifaceted insights into insect physiology and insecticide susceptibility, thereby offering valuable insights for the development of effective strategies to mitigate the impact of this destructive pest.</p></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100569"},"PeriodicalIF":6.8,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002227/pdfft?md5=727cbfc704ab4ac5a7cd9f6da139bc1b&pid=1-s2.0-S2667064X24002227-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142044902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivating resilience: Harnessing pyoverdine-producing Pseudomonas to contrast iron deficiency in cucumber plants","authors":"José María Lozano-González ,&nbsp;Mónica Yorlady Alzate Zuluaga ,&nbsp;Juan José Lucena ,&nbsp;Sandra López-Rayo ,&nbsp;Sonia Monterisi ,&nbsp;Stefano Cesco ,&nbsp;Youry Pii","doi":"10.1016/j.stress.2024.100565","DOIUrl":"10.1016/j.stress.2024.100565","url":null,"abstract":"<div><p>Iron (Fe) deficiency in crops significantly reduces yield, impacting agricultural productivity worldwide. Synthetic Fe chelates are commonly applied as fertilizers to address this issue, but their synthetic nature and prolonged use poses environmental risks. Thus, inoculation of plant growth-promoting bacteria rises as an alternative to enhance Fe uptake in crops while minimizing reliance on synthetic chelates. This study aimed to examine the influence of <em>Pseudomonas</em> RMC4 inoculation and pyoverdine application on cucumber plants cultivated hydroponically under Fe deficiency conditions. Evaluations included the SPAD index, plant biomass, root morphology, Fe-chelate reductase activity, gene expression, and ionomic analysis. Following Fe deficiency, <em>Pseudomonas</em> RMC4 inoculation improved the SPAD index, increased dry weight, enhanced root development, and facilitated Fe acquisition mechanisms, thus, fostering the endogenous resilience of the plant to the limited Fe availability. This improvement was observed with bacterial inoculation or pyoverdine application alongside an insoluble Fe source (ferrihydrite). Overall, the results suggest the beneficial impact of <em>Pseudomonas</em> RMC4 inoculation in alleviating symptoms of Fe deficiency. Future studies will investigate bacterial application under field conditions to assess its potential in replacing synthetic Fe-chelates fertilizers in crop production in favor of more sustainable agriculture.</p></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100565"},"PeriodicalIF":6.8,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002185/pdfft?md5=e5606005577f5f71c9fef4d1031dc3dd&pid=1-s2.0-S2667064X24002185-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142012070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of biostimulants for water stress mitigation in two durum wheat (Triticum durum Desf.) genotypes with different drought tolerance","authors":"Matteo Spada ,&nbsp;Miriam Marín-Sanz ,&nbsp;Valentina Bigini ,&nbsp;Giulia Quagliata ,&nbsp;Eleonora Coppa ,&nbsp;Francisco Barro ,&nbsp;Daniel Savatin ,&nbsp;Roberto Ruggeri ,&nbsp;Francesco Sestili ,&nbsp;Francesco Rossini ,&nbsp;Stefania Astolfi","doi":"10.1016/j.stress.2024.100566","DOIUrl":"10.1016/j.stress.2024.100566","url":null,"abstract":"<div><p>Agriculture is facing complex and unprecedented challenges, such as climate change and global population growth, associated with the increase of food demand. Durum wheat (DW) is a strategic crop for food security in the Mediterranean region. In this work, the effect of two biostimulant compounds on drought response of two DW genotypes with different drought tolerance degree was evaluated: the tolerant genotype Svems16 and the sensitive cv. Iride. Genotyping-By-Sequencing analysis allowed to identify variants in response to water-related genes, such as those encoding dehydrins, with Svems16 exhibiting a missense variant divergent from the sequence found in Iride. Drought significantly hindered growth of cv. Iride by increasing oxidative stress and diminishing stomata density. This difficulty was mitigated by the application of biostimulants, which induced root morphological changes, and increased stomata density. On the other hand, the growth of Svems16 seedlings was not significantly affected by drought, confirming its significant degree of tolerance toward that stress compared with Iride. As a result, both biostimulants showed limited efficacy when applied to Svems16 stressed plants. In conclusion, the application of biostimulants emerges as a valuable agronomic strategy for mitigating drought stress in sensitive DW cultivars.</p></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100566"},"PeriodicalIF":6.8,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002197/pdfft?md5=0f6e624f5cbd126aa5c201865952d2e8&pid=1-s2.0-S2667064X24002197-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142021100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proline and glycine betaine: A dynamic duo for enhancing salt stress resilience in maize by regulating growth, Stomatal size, and Oxidative stress responses","authors":"Mohammad Saidur Rhaman ,&nbsp;Farjana Rauf ,&nbsp;Shaila Shermin Tania ,&nbsp;Nafiz Bayazid ,&nbsp;Md Tahjib-ul-Arif ,&nbsp;Arif Hasan Khan Robin ,&nbsp;Md Anamul Hoque ,&nbsp;Xinghong Yang ,&nbsp;Yoshiyuki Murata ,&nbsp;Marian Brestic","doi":"10.1016/j.stress.2024.100563","DOIUrl":"10.1016/j.stress.2024.100563","url":null,"abstract":"<div><p>Osmolytes proline (Pro) and glycine betaine (GB) have been reported to impart tolerance against salinity stress in many plants. However, there is no report available on the combined application of Pro and GB to mitigate salinity-induced growth inhibition in maize. Consequently, the goal of the current study is to assess Pro's and GB's potential as priming and exogenous agents in maize under salt stress. Therefore, the present study was conducted using a petri dish and hydroponic pot experiment to evaluate the morpho-physiological and biochemical characteristics of maize plants subjected to salt stress, with the addition of Pro and GB. The seeds of maize were germinated under 20 mM of each Pro or GB, with or without 120 mM salinity stress. The findings demonstrated that salt stress lessened the final germination percentage (FGP) (52.9 %), photosynthetic pigments (40.0 %), relative water content (RWC) (20.2 %), stomatal size (59.3 %), and leaf and root <em>K</em>⁺/Na⁺ ratios of maize seedlings compared to control. In addition, compared to the control, higher levels of malondialdehyde (MDA) (24.2 %) and hydrogen peroxide (H₂O₂) (25.7 %) were observed, whereas lower levels of catalase (CAT) (43.8 %) and peroxidase (POX) (30.4 %) were noted. The priming with Pro, GB, Pro+GB significantly increased FGP, germination index (GI), shoot-root biomass, seed vigor index (SVI), and reduced mean germination time (MGT) under salt stress. Foliar application of Pro, GB, or Pro+GB resulted in a significant increment in chlorophyll contents, RWC, <em>K</em>⁺ absorption, and stomatal size under salt stress. Furthermore, supplementing with Pro, GB, or Pro+GB reduced the accumulation of H₂O₂ (24.0, 23.3, and 31.1 %, respectively) and MDA (22.8, 17.2, and 32.1 %, respectively) caused by salt and augmented the levels of CAT (33.3, 22.8, and 45.2 %, respectively) and POX (36.4, 23.5, and 47.2 %, respectively) in the leaves. Taken together, the current study's findings indicate that combining Pro and GB is one of the most effective techniques for improving the salinity-tolerant seed germination and seedling traits of maize plants. Consequently, this study recommends that Pro and GB can be used as seed priming and exogenous agents to help maize grow faster in salt-stress situations.</p></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100563"},"PeriodicalIF":6.8,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002161/pdfft?md5=722e713f864e3f7f50f4656f6b974a54&pid=1-s2.0-S2667064X24002161-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of rice-husk biochar on soil attributes, microbiome interaction and functional traits of radish plants: A smart candidate for soil engineering","authors":"Kaushik Gautam ,&nbsp;Priya Singh ,&nbsp;Rajeev Pratap Singh ,&nbsp;Anita Singh","doi":"10.1016/j.stress.2024.100564","DOIUrl":"10.1016/j.stress.2024.100564","url":null,"abstract":"<div><p>This study is based on the application of different doses of rice husk biochar; RHB (1, 5, 10, 15, and 20 t/ha) in wastewater-irrigated soil to observe its impact on the soil qualities and functional attributes of the radish plants. The results showed that the RHB treatments increased the soil pH, electrical conductivity (EC), bulk density (BD), soil organic carbon (SOC), total nitrogen (TN), available phosphorous (AP), enzymatic activities, and microbial biomass in the soil. The soil metagenome analysis at 15 t/ha showed more abundance of microbial communities like Actinobacteria, Acidobacteria<em>,</em> and Nitrospira over to control soil<em>.</em> On the other hand, more significantly it led to a reduction in the availability of Cd, Cr, Ni, Zn, and Cu by 73, 60, 63, 61, and 68 %, respectively maximally at 20 t/ha dose of biochar application. Along with that it also led to the level of the all the toxic heavy metals below their safe limit in the edible part of the radish plants. All the doses of RHB application have resulted improvements in functional attributes of the radish plants by reducing the production of oxidative biomarkers more significantly at 15 and 20 t/ha doses of biochar compared to the control plants. Among all the doses the biomass of edible parts of plant i.e., root was increased and the maximum increment was found at 15 t/ha over to control plants and later on, there was an insignificant difference. The total concentrations of Cd, Cr, Ni, Zn, and Cu were decreased in the root and shoot of the plants in a dose-dependent manner. The correlation biplot also showed that with the amendment of biochar, there was a significant correlation between the soil properties and plant yield.</p></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100564"},"PeriodicalIF":6.8,"publicationDate":"2024-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002173/pdfft?md5=f63f6df8384a643d6ae549935591b431&pid=1-s2.0-S2667064X24002173-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142012069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling metabolomics and antioxidant potential of sweet orange cultivar Pusa Sharad grafted on various citrus rootstocks under sodium chloride stress","authors":"Kripa Shankar ,&nbsp;Om Prakash Awasthi ,&nbsp;Supradip Saha ,&nbsp;Jai Prakash ,&nbsp;Renu Pandey ,&nbsp;Theivanai Murugan ,&nbsp;Aria Dolatabadian","doi":"10.1016/j.stress.2024.100562","DOIUrl":"10.1016/j.stress.2024.100562","url":null,"abstract":"<div><p>Rootstock as grafting material mitigates sodium chloride (NaCl) stress by altering physiological, metabolite, and gene expression patterns across different genotypes, cultivars, or species. In this study, we investigated the antioxidant and metabolic responses of sweet orange cultivar Pusa Sharad (PS) grafted onto various rootstocks: Jatti Khatti (JK), X–639 (X9), CRH–12 (C12), NRCC–1 (N1), NRCC–2 (N2), NRCC–3 (N3), NRCC–4 (N4), NRCC–5 (N5), Troyer citrange (TC), CRH–47 (C47), and Cleopatra mandarin (CM). These responses were assessed under different salinity stress levels. Biochemical parameters, including sugars, proline, phenol, soluble protein, hydrogen peroxide (H₂O₂), superoxide radicals (O₂^–), lipid peroxidation, catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), peroxidase (POD), vitamin C, vitamin E, organic acids, and fatty acids were analysed. Results demonstrated that the accumulation of H₂O₂, O₂^–, and malondialdehyde (MDA) was upregulated in PS grafted onto the TC, JK, N2, and C12 rootstocks. Conversely, the CAT, SOD, APX, vitamin C, and vitamin E contents were notably higher in PS grafted onto CM, X9, and C47 under 60 mM NaCl stress. Metabolomic analysis indicated that trehalose, raffinose, sucrose, D-galactose, myo-inositol, piperazine, acetic acid, malonic acid, palmitic acid, stearic acid, and pentanoic acid played crucial roles in metabolic adjustments under increasing NaCl stress. Furthermore, PS grafted onto CM, C47, X9, N1, or N3 showed greater tolerance to NaCl compared to those grafted onto JK, C12, N2, N4, N5, or TC, making these combinations adaptable upto 60 mM NaCl concentrations. This study highlights the role of potential metabolites and its use in enhancing NaCl tolerance through grafting onto tolerant rootstocks.</p></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100562"},"PeriodicalIF":6.8,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X2400215X/pdfft?md5=abc4926a49a89351592b593aebfcdd0b&pid=1-s2.0-S2667064X2400215X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142040218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stable early heading in photoperiod-insensitive rice varieties results from an extremely short photoperiod-sensitive phase and weak temperature sensitivity","authors":"Shuntaro Sakaguchi,&nbsp;Md. Imdadul Hoque,&nbsp;Yuji Kishima","doi":"10.1016/j.stress.2024.100561","DOIUrl":"10.1016/j.stress.2024.100561","url":null,"abstract":"<div><p>The transition from vegetative to reproductive growth in rice (<em>Oryza sativa</em>) is mainly regulated by photoperiod and temperature; however, it remains unclear how photoperiod-insensitive (photo-In) early heading rice varieties make this transition. Vegetative growth consists of a basic vegetative phase (BVP) and a photoperiod-sensitive phase (PSP). A certain duration of the BVP is required prior to the PSP, which varies according to daylength conditions. PSP duration is conventionally used as a parameter of photoperiod sensitivity, but this is not applicable to photo-In rice varieties. Here, we aimed to characterize the PSP in photo-In rice varieties. We examined four photo-In varieties and photoperiod-sensitive (photo-Se) varieties grown in four controlled environments, evaluating the lengths of their growth phases based on the turning point of the interval of leaf emergence and panicle initiation (PI). The photo-In varieties had an extremely short PSP, regardless of daylength, compared with the photo-Se varieties. Low temperature uniformly prolonged all growth stages in the photo-In varieties but led to a much longer PSP in the photo-Se varieties. The photo-In varieties, each carrying a non-functional allele of <em>Ghd7</em>, lost the ability to suppress PI, resulting in early heading. Hemi-knockout plants at the <em>Ghd7</em> locus demonstrated the earlier heading than the wild-type plant due to shortening of PSP. Our results suggest that the photo-In varieties begin PI immediately after the end of the BVP, but their flowering is not a temperature-dependent process.</p></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"13 ","pages":"Article 100561"},"PeriodicalIF":6.8,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667064X24002148/pdfft?md5=1fc7ebce2e3026154b009bb941d65e2a&pid=1-s2.0-S2667064X24002148-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141979698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}