Plant Stress最新文献

{"title":"Auxin accumulation mediated by the CsPIF1-like-CsYUC8 module drives high temperature-induced hypocotyl elongation in cucumber","authors":"Yonggui Liang ,&nbsp;Ziyan Feng ,&nbsp;Bingwei Yu ,&nbsp;Simin Yang ,&nbsp;Changwen Gao ,&nbsp;Renjian Liu ,&nbsp;Zhilei Xia ,&nbsp;Yujia Lin ,&nbsp;Liping Chen ,&nbsp;Zhengkun Qiu ,&nbsp;Bihao Cao ,&nbsp;Shuangshuang Yan","doi":"10.1016/j.stress.2025.100991","DOIUrl":"10.1016/j.stress.2025.100991","url":null,"abstract":"<div><div>High temperature trigger various thermomorphogenic adaptations in plants, such as hypocotyl elongation, which disrupts developmental homeostasis and threaten agricultural productivity. However, the molecular mechanism underlying hypocotyl elongation in cucumber under high-temperature conditions remain poorly characterized. Here, we revealed that the increased auxin biosynthesis is a pivotal driver of high-temperature-induced hypocotyl elongation in cucumber. Transcript profiling indicated that <em>CsYUC8</em>, a gene encoding a key auxin biosynthetic enzyme, was upregulated in cucumber hypocotyl elongation under high-temperature conditions. Functional analysis and NPA (auxin inhibitor) treatments demonstrated that <em>CsYUC8-</em>mediated auxin accumulation is essential for thermomorphogenic hypocotyl elongation. Furthermore, we identified a heat-responsive bHLH transcription factor, <em>CsPIF1-like</em>, which directly binds to the <em>CsYUC8</em> promoter to activate its expression. Those results revealed a CsPIF1-like-<em>CsYUC8</em> regulatory module that fine-tunes auxin-dependent hypocotyl elongation in cucumber, providing a mechanistic framework for improving heat resilience in cucurbit crops.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 100991"},"PeriodicalIF":6.8,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of the Dof gene family in Carya illinoinensis and potential function analysis of CiDof22 in drought stress","authors":"Jinhua He,&nbsp;Linna Wang,&nbsp;Lina Zou,&nbsp;Zixian Yao,&nbsp;Shunran Zhang,&nbsp;Yan Xiang","doi":"10.1016/j.stress.2025.100990","DOIUrl":"10.1016/j.stress.2025.100990","url":null,"abstract":"<div><div>DNA-binding with one finger (Dof) proteins are a unique class of plant-specific transcription factors that play a crucial role in regulating plant growth, development, and stress responses. <em>Carya illinoinensis</em> is an economically and ecologically important tree species. However, the functions of <em>Dof</em> transcription factors in this species remain unknown. In this study, 48 members of the <em>Dof</em> transcription factor family were identified from the <em>C. illinoinensis</em> genome and comprehensively investigated using bioinformatics. The analyses included phylogenetic relationships, chromosomal distribution, gene structure organization, conserved motif patterns, promoter cis-regulatory elements, interspecies collinearity, and evolutionary rate assessments. The integration of transcriptome data with qRT-PCR analysis revealed that <em>CiDof22</em> exhibited a strong response to drought stress. CiDof22 was demonstrated as a nuclear-localized protein that lacks transcriptional self-activation activity in yeast. By transforming <em>CiDof22</em> into <em>Nicotiana benthamiana</em> and using a 10% PEG6000 solution to simulate drought conditions for transient expression experiments, we found that both <em>CiDof22</em> and drought-related genes were upregulated, confirming that <em>CiDof22</em> significantly responds to drought stress. The findings of this study enhance our understanding of <em>CiDof</em> gene structure and function, while identifying promising gene candidates for improving drought resistance in <em>C. illinoinensis</em> through molecular breeding.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 100990"},"PeriodicalIF":6.8,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144851923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptome and defense hormone analyses of resistant and susceptible tea plant varieties revealed the response mechanisms of tea plants to Apolygus lucorum feeding","authors":"Yuan-Hong Wan ,&nbsp;Chun Yang ,&nbsp;Da-He Qiao ,&nbsp;Yue-Xin Li ,&nbsp;Xiao-Zeng Mi ,&nbsp;Xing-Yun Shi ,&nbsp;Shuai Li ,&nbsp;Jin-Feng Zhang ,&nbsp;Yu-He Wan ,&nbsp;Ze-Hong Meng","doi":"10.1016/j.stress.2025.100989","DOIUrl":"10.1016/j.stress.2025.100989","url":null,"abstract":"<div><div><em>Apolygus lucorum</em> represents a major insect pest affecting <em>Camellia sinensis</em>, with its feeding activity posing significant threats to tea plant growth and yield productivity. However, the molecular basis of tea plant resistance against <em>A. lucorum</em> infestation remains poorly characterized. In this study, we employed two distinct tea varieties, the resistant variety <em>Taixuan 0310</em> (TX0310) and the susceptible variety <em>Huangjinya</em> (HJY), for comparative analysis. Phenotypic evaluation revealed consistently less severe damage symptoms in TX0310 compared to HJY under identical infestation conditions. Through transcriptome sequencing approaches and integrated phytohormone profiling, we systematically elucidated the differential defense responses to <em>A. lucorum</em>. The resistant variety TX0310 exhibited sustained and comprehensive activation of both jasmonic acid (JA) signaling cascades and secondary metabolic pathways, whereas the susceptible variety HJY displayed only transient and attenuated JA responses. Notably, key genes involved in JA biosynthesis (<em>CsOPR11</em> and <em>CsJMT</em>) showed marked upregulation specifically in TX0310. Quantitative analysis confirmed significantly higher accumulation of defense-related phytohormones including JA and salicylic acid (SA) in TX0310 relative to HJY. Through weighted gene co-expression network analysis (WGCNA) and Pearson correlation analysis, we identified 10 core regulatory genes (including <em>CsOPR11, CsAOS</em>, and <em>CsJMT</em>). Notably, overexpression of the <em>CsOPR11</em> gene in tobacco resulted in significantly higher methyl jasmonate (MeJA) levels in <em>CsOPR11</em>-transgenic plants compared to wild-type controls. This provides novel insights into the molecular mechanisms underlying tea plant resistance against insect pests.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 100989"},"PeriodicalIF":6.8,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Divergent evolution of AWPM-19 from bryophytes to angiosperms and functional conservation of AtAWPM-19-1 and PtAWPM-19-4 in drought response","authors":"Cheng Wang,&nbsp;Xiaoyu Liang,&nbsp;Lingli Dong,&nbsp;Wen Sun,&nbsp;Yanglin Liu,&nbsp;Xinyuan Zhang,&nbsp;Liping Zhang,&nbsp;Jinbiao Li,&nbsp;Hanwei Yan","doi":"10.1016/j.stress.2025.100987","DOIUrl":"10.1016/j.stress.2025.100987","url":null,"abstract":"<div><div>The AWPM-19 gene family, originally identified as an ABA-induced 19-kDa polypeptide in wheat plasma membranes, is involved in stress response pathways, particularly in drought tolerance. However, comprehensive analyses of this gene family are scarce, and the functional characterization of its members remains limited. In this study, we performed genome-wide analysis of the AWPM-19 gene family across ten distantly related plant species, ranging from bryophytes to angiosperms. We analyzed their molecular features, motif compositions, cis-acting regulatory elements in promoters, evolutionary relationships, and synteny across species. The results revealed that AWPM-19 genes in <em>Marchantia polymorpha, Physcomitrium patens</em> and <em>Selaginella moellendorffii</em> clustered together, indicating close evolutionary relationships within non-seed plants. In contrast, genes from <em>Amborella trichopoda, Nymphaea colorata, Arabidopsis thaliana, Brachypodium distachyon, Oryza sativa, Vitis vinifera</em> and <em>Populus trichocarpa</em> showed higher sequence similarity and closer phylogenetic relationship. To explore the functional role of AWPM-19 genes, overexpression lines of <em>PtAWPM-19-4</em> in <em>Arabidopsis, atawpm-19-1</em> mutants, and <em>PtAWPM-19-4/atawpm-19-1</em> complementation lines were generated. Functional analysis revealed both <em>PtAWPM-19-4</em> and <em>AtAWPM-19-1</em> are involved in drought tolerance. These findings provide new insights into the evolutionary conservation and functional relevance of AWPM-19 genes in plant drought stress adaptation.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 100987"},"PeriodicalIF":6.8,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144841907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agrobacterium tumefaciens-induced proteomic remodelling and physiological adaptations in Hypericum perforatum L","authors":"Preeti Shakya ,&nbsp;Rajendran K. Selvakesavan ,&nbsp;Dawid Perlikowski ,&nbsp;Vimala Antonydhason ,&nbsp;Leonard Kiirika ,&nbsp;Gregory Franklin","doi":"10.1016/j.stress.2025.100986","DOIUrl":"10.1016/j.stress.2025.100986","url":null,"abstract":"<div><div>Despite the widespread use of <em>Agrobacterium tumefaciens</em>-mediated transformation in plant genetic engineering and functional genomics, many economically and industrially important crops, especially medicinal plants, are resistant to <em>A. tumefaciens</em>-mediated T-DNA transfer and have proven difficult to improve with this technique. Here, we report, for the first time, the proteomic and physiological changes in a pharmaceutically important and non-transformable medicinal plant, <em>Hypericum perforatum</em>, in response to <em>A. tumefaciens</em> using a comprehensive proteomic approach and physiological assessments. Proteome analysis of <em>H. perforatum</em> cells challenged with <em>A. tumefaciens</em> via liquid chromatography/tandem mass spectrometry revealed 1200 differentially expressed proteins (DEPs; 613 up-regulated and 587 down-regulated) after 12 h and 390 DEPs (174 up-regulated and 216 down-regulated) after 24 h of co-cultivation. Functional analysis revealed early activation of the key defence-related protein PR-10, indicating rapid pathogen recognition and initiation of defence responses. Simultaneously, antioxidant enzymes, such as peroxiredoxin and l-ascorbate peroxidase, were significantly up-regulated, indicating robust attenuation of oxidative stress. The induction of cell wall-modifying proteins, such as UDP-arabinopyranose mutase and pectin acetylesterases as well as accumulation of proteins related to secondary metabolic defence mechanisms, such as phenylalanine ammonia lyase, 1,3,7-trihydroxyxanthone synthase, and benzophenone synthase, indicated a coordinated plant defence response. Physiological analysis also revealed significant stress responses in <em>H. perforatum</em> seedlings, including reduced photosynthetic performance and stomatal changes as early as 12 h, which intensified after 24 h. Taken together, the identified key proteins and altered physiological responses of <em>H. perforatum</em> contribute to the understanding of factors underlying recalcitrance of plants to <em>Agrobacterium</em>-mediated transformation.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 100986"},"PeriodicalIF":6.8,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144861238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GhSPDS11 up-regulated spermidine synthase responding to alkaline tolerance in cotton","authors":"Hao Lan ,&nbsp;Maohua Dai ,&nbsp;Xiugui Chen ,&nbsp;Juyun Zheng ,&nbsp;Ruize Song ,&nbsp;Xuke Lu ,&nbsp;Fange Wu ,&nbsp;Xin Yu ,&nbsp;Xinrui Zhang ,&nbsp;Zhining Yang ,&nbsp;Menghao Zhang ,&nbsp;Lixue Guo ,&nbsp;Shuai Wang ,&nbsp;Lanjie Zhao ,&nbsp;Yupeng Cui ,&nbsp;Xue-Rong Zhou ,&nbsp;Xueyuan Li ,&nbsp;Wuwei Ye","doi":"10.1016/j.stress.2025.100988","DOIUrl":"10.1016/j.stress.2025.100988","url":null,"abstract":"<div><div>Alkaline stress causes significant adverse effects that slows down the growth of plants and lowers the yield of crops; hence, it is a major challenge in cotton farming. Spermidine (Spd), a vital polyamine, plays a significant role in enhancing plant resistance to stress caused by various abiotic factors. The molecular mechanism of Spd biosynthesis and especially the role of spermidine synthase (SPDS) in tolerance of alkaline stress in cotton is, however, little known. In this study, a systematic comparative analysis of SPDS-associated genes was performed across four representative cotton cultivars (<em>Gossypium</em> spp.), followed by preliminary functional characterization through promoter <em>cis</em>-acting element profiling. Virus-induced gene silencing (VIGS) was utilized to disrupt <em>GhSPDS11</em>-mediated Spd biosynthesis. Under alkaline stress, <em>GhSPDS11</em>-silenced seedlings exhibited 29.14% and 11.12% reductions in superoxide dismutase (SOD) and catalase (CAT) activities, 31.57% and 15.16% decreases in soluble sugar and proline (Pro) content, along with 42.38% and 38.66% increases in malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) compared to controls. Concurrently, silenced plants showed 44.87% fewer open stomata and significant declines in Spd content, relative water content, and biomass. These results indicate the key importance of Spd, which is composed of <em>GhSPDS11</em>, in improving alkali tolerance in cotton. This research gives good information regarding the molecular processes that take part in the tolerance of cotton to the saline-alkaline soils, and that <em>GhSPDS11</em> could be a good genetic target in cotton enhancement in this tough agro-climatic condition.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 100988"},"PeriodicalIF":6.8,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144827741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generational effects of biosynthesized titanium dioxide nanoparticles on bitter gourd growth and phytochemistry","authors":"Bimal Das ,&nbsp;Bappa Paramanik ,&nbsp;Dipak Kumar Murmu ,&nbsp;Biman De ,&nbsp;Partha Sarathi Patra ,&nbsp;Prithwiraj Dey ,&nbsp;Pradip Dey","doi":"10.1016/j.stress.2025.100985","DOIUrl":"10.1016/j.stress.2025.100985","url":null,"abstract":"<div><div>The biosynthesis titanium dioxide nanoparticles (TiO₂ NPs) on seedling growth, yield, biochemical traits, and their transmission effect over two generations were assessed in bitter gourd cv. Megna 2. Six distinct nanoscale TiO₂ concentrations (0, 10, 20, 50, 80, and 100 mg L^-1) were applied to bitter gourd seeds, along with a control. Various characterization methods, including dynamic light scattering (DLS), UV–visible spectroscopy, fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM), were utilized to verify the dimensions, absorption spectra, functional molecules and surface structure of biosynthesis TiO₂ NPs. The initial study observed that the nanoparticle-treated seeds produced better germination and seedling growth (p &lt; 0.05) than the control. Treatments with TiO₂ NPs at the seedling stage had a substantial effect on catalase and peroxidase activities. Parental TiO₂ NPs treated seeds ‘N1’ (first generation) and their progeny ‘N2’ (second generation) showed substantial changes in plant growth, fruit yield characteristics, and phytomedicine properties in bitter gourd. Concentration with 80 mg L^-1 over the two generations proved to be the best treatment, which significantly increased total chlorophyll content, ascorbic acid content, TSS content, beta carotene content, charantin contents, and cucurbitacin content, respectively, compared to the control. After two generations of verification of TiO₂ NP uptake in roots and shoots, it was discovered that the quantity of particle accumulation in the next generation had decreased but significant results achieved for growth and phytomedicinal properties in bitter gourd. These findings suggested that biosynthesized TiO₂ NPs can induce stable transgenerational enhancement offering promising applications in sustainable agricultural by improving both productivity and nutritional quality in edible crop plants.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 100985"},"PeriodicalIF":6.8,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144858394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of magnetic silver/zinc/iron nanocomposite mitigates detrimental effects of polymethyl methacrylate nanoplastics and Arsenic and ameliorates biochemical compositions in Triticum aestivum L","authors":"Sevda Alizadeh ,&nbsp;Latifeh Pourakbar ,&nbsp;Sina Siavash Moghaddam","doi":"10.1016/j.stress.2025.100984","DOIUrl":"10.1016/j.stress.2025.100984","url":null,"abstract":"<div><div>With the phytotoxicity of nano-microplastics and heavy metals, it becomes imperative to develop appropriate solutions to prevent the entry of these pollutants into plants and to mitigate their harmful effects. The silver/zinc/iron green magnetic nanocomposite (Ag/Zn/Fe) was synthesized using <em>Malva Sylvestris</em> plant extract and characterized by TEM, XRD, FESEM. To explore the effects of Arsenic (As: 50 or 100 mg/L), polymethyl methacrylate nanoplastics (PMMANPs: 50 or 500 mg/L), and Ag/Zn/Fe (100 mg/L) in the wheat plant medium, a factorial experiment employing a randomized complete block design was conducted. TEM images showed the presence of PMMANPs in the roots and leaves, and Ag/Zn/Fe in the leaves. The results indicated that PMMANPs and Ag/Zn/Fe reduced As absorption into the plants. Both As and PMMANPs increased total phenol content (TPC), total flavonoid content (TFC), and DPPH radical scavenging activity. They also increased hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) content. Ag/Zn/Fe improved wheat tolerance by reducing MDA and H₂O₂, TPC and TFC under As and PMMANPs. Polyphenol profile of leaves was changed by all treatments. As increased cinnamic acid, caffeic acid, gallic acid, chlorogenic acid, and p-coumaric, and As+PMMANPs enhanced rosmarinic acid, quercetin and chlorogenic acid. Ag/Zn/Fe increased gallic acid and chlorogenic acid in non-stressed treatments, rosmarinic acid and chlorogenic at 50 mg/L As, and quercetin and caffeic acid at 100 mg/L As. These results suggest that Ag/Zn/Fe mitigated the adverse effects of As and PMMANPs in wheat plants.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"17 ","pages":"Article 100984"},"PeriodicalIF":6.8,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144840850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigating salt stress in Lens culinaris: The protective mechanism of Rhizobium in enhancing growth, photosynthesis, and antioxidant defense system","authors":"Rinkee Kumari ,&nbsp;Ekta Pandey ,&nbsp;Shahla Faizan ,&nbsp;Ahlam Khalofah ,&nbsp;Mohammad Faizan","doi":"10.1016/j.stress.2025.100983","DOIUrl":"10.1016/j.stress.2025.100983","url":null,"abstract":"<div><div>Agricultural soil contamination, particularly salinization, poses a significant and growing threat to global crop productivity, thereby necessitating the development of sustainable strategies to mitigate its adverse effects. This study investigates the potential of Rhizobium inoculation to alleviate the detrimental impact of salinity stress on <em>Lens culinaris</em>. Rhizobium plays a crucial role in legume development through symbiotic nitrogen fixation and may also enhance plant growth under stress conditions by engaging in complex biochemical interactions. However, research on its ability to activate abiotic stress signaling pathways and induce physiological changes in plants remains limited. To address this, L. <em>culinaris</em> plants were subjected to varying concentrations of NaCl (50 mM and 150 mM) at 20 days post-germination, with and without Rhizobium inoculation. For inoculation, 100 g of jaggery or sugar was dissolved in 500 ml of water, boiled, cooled, and then used to mix the Rhizobium culture. This mixture was used to coat lentil seeds, which were air-dried in the shade before sowing. Salt stress significantly reduced plant growth, physiological parameters, and yield. In contrast, Rhizobium inoculation improved reactive oxygen species (ROS) balance and enhanced the activities of key antioxidant enzymes, including superoxide dismutase (SOD) by 21.0 %, catalase (CAT) by 11.34 %, and peroxidase (POD) by 10.43 %. Additionally, photosynthetic rate and chlorophyll content were increased by 10.08 %, stomatal conductance was improved, and leghemoglobin content rose by 15.90 %. Protein and proline levels were elevated by 18.31 % and 32.57 %, respectively. Moreover, membrane stability was enhanced, sodium accumulation was reduced, and overall yield and stomatal behavior were optimized under saline conditions. Rhizobium inoculation also directly supported ACC deaminase (ACCD) activity and increased indole-3-acetic acid (IAA) production in both plant tissues and bacterial isolates. These findings underscore the potential of Rhizobium in mitigating salt-induced damage in L. <em>culinaris</em> by modulating antioxidant enzyme systems, enhancing photosynthetic performance, and promoting redox homeostasis.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 100983"},"PeriodicalIF":6.8,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144827728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles in sustainable agriculture: enhancing nutrient use efficiency and abiotic stress resilience under climate change","authors":"Yingfen Yang ,&nbsp;Chenghu Ye ,&nbsp;Meiwei Zhao ,&nbsp;Juan Li ,&nbsp;Xiaoxia Zhang ,&nbsp;Zihui Yang ,&nbsp;Zhibo Yang ,&nbsp;Uthman Balgith Algopishi ,&nbsp;Waqar Ahmed","doi":"10.1016/j.stress.2025.100982","DOIUrl":"10.1016/j.stress.2025.100982","url":null,"abstract":"<div><div>Climate change poses significant challenges to global agriculture, particularly by reducing nutrient availability and crop yields. Improving nutrient absorption and utilization is vital for sustainable agriculture, especially as the global population grows and food security becomes increasingly critical. Nanoparticles (NPs) offer a promising solution by enhancing plant nutrient uptake and stress tolerance through their unique physicochemical properties. This review examines the impact of various NP types: carbon-based, metal-based, and silicon-based applied through foliar sprays, soil amendments, or seed priming. These methods can improve nutrient solubility, root development, and crop productivity under abiotic stresses like drought, salinity, and heavy metal toxicity. We present recent case studies and experimental findings that highlight NPs’ dual role in enhancing macro/micronutrient bioavailability and mitigating stress via antioxidant activity and osmotic regulation. Nano-fertilizers also optimize nutrient use efficiency through controlled release, reducing environmental losses. However, challenges such as ecosystem safety, regulatory frameworks, and economic viability must be addressed for large-scale adoption. This review explores NPs potential to improve plant growth and nutrient uptake under climate change conditions, emphasizing the need for further research to ensure safe, sustainable implementation.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"17 ","pages":"Article 100982"},"PeriodicalIF":6.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144826839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}