Divergent evolution of AWPM-19 from bryophytes to angiosperms and functional conservation of AtAWPM-19-1 and PtAWPM-19-4 in drought response

IF 6.8 Q1 PLANT SCIENCES
Cheng Wang, Xiaoyu Liang, Lingli Dong, Wen Sun, Yanglin Liu, Xinyuan Zhang, Liping Zhang, Jinbiao Li, Hanwei Yan
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引用次数: 0

Abstract

The AWPM-19 gene family, originally identified as an ABA-induced 19-kDa polypeptide in wheat plasma membranes, is involved in stress response pathways, particularly in drought tolerance. However, comprehensive analyses of this gene family are scarce, and the functional characterization of its members remains limited. In this study, we performed genome-wide analysis of the AWPM-19 gene family across ten distantly related plant species, ranging from bryophytes to angiosperms. We analyzed their molecular features, motif compositions, cis-acting regulatory elements in promoters, evolutionary relationships, and synteny across species. The results revealed that AWPM-19 genes in Marchantia polymorpha, Physcomitrium patens and Selaginella moellendorffii clustered together, indicating close evolutionary relationships within non-seed plants. In contrast, genes from Amborella trichopoda, Nymphaea colorata, Arabidopsis thaliana, Brachypodium distachyon, Oryza sativa, Vitis vinifera and Populus trichocarpa showed higher sequence similarity and closer phylogenetic relationship. To explore the functional role of AWPM-19 genes, overexpression lines of PtAWPM-19-4 in Arabidopsis, atawpm-19-1 mutants, and PtAWPM-19-4/atawpm-19-1 complementation lines were generated. Functional analysis revealed both PtAWPM-19-4 and AtAWPM-19-1 are involved in drought tolerance. These findings provide new insights into the evolutionary conservation and functional relevance of AWPM-19 genes in plant drought stress adaptation.
AWPM-19从苔藓植物到被子植物的分化演化及AtAWPM-19-1和PtAWPM-19-4在干旱响应中的功能保护
AWPM-19基因家族最初被鉴定为aba诱导的小麦质膜19 kda多肽,参与胁迫反应途径,特别是抗旱性。然而,对该基因家族的全面分析很少,其成员的功能表征仍然有限。在这项研究中,我们对从苔藓植物到被子植物等10种远亲植物的AWPM-19基因家族进行了全基因组分析。我们分析了它们的分子特征、基序组成、启动子中的顺式调控元件、进化关系以及物种间的合度。结果表明,多形地豆、专利立盆菌和卷柏的AWPM-19基因聚集在一起,表明在非种子植物中存在密切的进化关系。相比之下,三叶草(Amborella trichopoda)、花梨花(Nymphaea colorata)、拟南芥(Arabidopsis thaliana)、短尾草(Brachypodium distachyon)、水稻(Oryza sativa)、葡萄(Vitis vinifera)和毛杨(Populus trichocarpa)的基因序列相似性更高,系统发育关系更密切。为了探究AWPM-19基因的功能作用,我们在拟南芥中构建了PtAWPM-19-4过表达系、atawpm-19-1突变体和PtAWPM-19-4/atawpm-19-1互补系。功能分析表明,PtAWPM-19-4和AtAWPM-19-1均参与了抗旱性调控。这些发现为AWPM-19基因在植物干旱胁迫适应中的进化保护和功能相关性提供了新的见解。
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来源期刊
Plant Stress
Plant Stress PLANT SCIENCES-
CiteScore
5.20
自引率
8.00%
发文量
76
审稿时长
63 days
期刊介绍: The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.
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