{"title":"Divergent evolution of AWPM-19 from bryophytes to angiosperms and functional conservation of AtAWPM-19-1 and PtAWPM-19-4 in drought response","authors":"Cheng Wang, Xiaoyu Liang, Lingli Dong, Wen Sun, Yanglin Liu, Xinyuan Zhang, Liping Zhang, Jinbiao Li, Hanwei Yan","doi":"10.1016/j.stress.2025.100987","DOIUrl":null,"url":null,"abstract":"<div><div>The AWPM-19 gene family, originally identified as an ABA-induced 19-kDa polypeptide in wheat plasma membranes, is involved in stress response pathways, particularly in drought tolerance. However, comprehensive analyses of this gene family are scarce, and the functional characterization of its members remains limited. In this study, we performed genome-wide analysis of the AWPM-19 gene family across ten distantly related plant species, ranging from bryophytes to angiosperms. We analyzed their molecular features, motif compositions, cis-acting regulatory elements in promoters, evolutionary relationships, and synteny across species. The results revealed that AWPM-19 genes in <em>Marchantia polymorpha, Physcomitrium patens</em> and <em>Selaginella moellendorffii</em> clustered together, indicating close evolutionary relationships within non-seed plants. In contrast, genes from <em>Amborella trichopoda, Nymphaea colorata, Arabidopsis thaliana, Brachypodium distachyon, Oryza sativa, Vitis vinifera</em> and <em>Populus trichocarpa</em> showed higher sequence similarity and closer phylogenetic relationship. To explore the functional role of AWPM-19 genes, overexpression lines of <em>PtAWPM-19-4</em> in <em>Arabidopsis, atawpm-19-1</em> mutants, and <em>PtAWPM-19-4/atawpm-19-1</em> complementation lines were generated. Functional analysis revealed both <em>PtAWPM-19-4</em> and <em>AtAWPM-19-1</em> are involved in drought tolerance. These findings provide new insights into the evolutionary conservation and functional relevance of AWPM-19 genes in plant drought stress adaptation.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 100987"},"PeriodicalIF":6.8000,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Stress","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667064X25002556","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The AWPM-19 gene family, originally identified as an ABA-induced 19-kDa polypeptide in wheat plasma membranes, is involved in stress response pathways, particularly in drought tolerance. However, comprehensive analyses of this gene family are scarce, and the functional characterization of its members remains limited. In this study, we performed genome-wide analysis of the AWPM-19 gene family across ten distantly related plant species, ranging from bryophytes to angiosperms. We analyzed their molecular features, motif compositions, cis-acting regulatory elements in promoters, evolutionary relationships, and synteny across species. The results revealed that AWPM-19 genes in Marchantia polymorpha, Physcomitrium patens and Selaginella moellendorffii clustered together, indicating close evolutionary relationships within non-seed plants. In contrast, genes from Amborella trichopoda, Nymphaea colorata, Arabidopsis thaliana, Brachypodium distachyon, Oryza sativa, Vitis vinifera and Populus trichocarpa showed higher sequence similarity and closer phylogenetic relationship. To explore the functional role of AWPM-19 genes, overexpression lines of PtAWPM-19-4 in Arabidopsis, atawpm-19-1 mutants, and PtAWPM-19-4/atawpm-19-1 complementation lines were generated. Functional analysis revealed both PtAWPM-19-4 and AtAWPM-19-1 are involved in drought tolerance. These findings provide new insights into the evolutionary conservation and functional relevance of AWPM-19 genes in plant drought stress adaptation.
期刊介绍:
The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues.
Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and:
Lack of water (drought) and excess (flooding),
Salinity stress,
Elevated temperature and/or low temperature (chilling and freezing),
Hypoxia and/or anoxia,
Mineral nutrient excess and/or deficiency,
Heavy metals and/or metalloids,
Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection,
Viral, phytoplasma, bacterial and fungal plant-pathogen interactions.
The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.