Agrobacterium tumefaciens-induced proteomic remodelling and physiological adaptations in Hypericum perforatum L

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2025-08-11 DOI:10.1016/j.stress.2025.100986

Preeti Shakya , Rajendran K. Selvakesavan , Dawid Perlikowski , Vimala Antonydhason , Leonard Kiirika , Gregory Franklin

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引用次数: 0

Abstract

Despite the widespread use of Agrobacterium tumefaciens-mediated transformation in plant genetic engineering and functional genomics, many economically and industrially important crops, especially medicinal plants, are resistant to A. tumefaciens-mediated T-DNA transfer and have proven difficult to improve with this technique. Here, we report, for the first time, the proteomic and physiological changes in a pharmaceutically important and non-transformable medicinal plant, Hypericum perforatum, in response to A. tumefaciens using a comprehensive proteomic approach and physiological assessments. Proteome analysis of H. perforatum cells challenged with A. tumefaciens via liquid chromatography/tandem mass spectrometry revealed 1200 differentially expressed proteins (DEPs; 613 up-regulated and 587 down-regulated) after 12 h and 390 DEPs (174 up-regulated and 216 down-regulated) after 24 h of co-cultivation. Functional analysis revealed early activation of the key defence-related protein PR-10, indicating rapid pathogen recognition and initiation of defence responses. Simultaneously, antioxidant enzymes, such as peroxiredoxin and l-ascorbate peroxidase, were significantly up-regulated, indicating robust attenuation of oxidative stress. The induction of cell wall-modifying proteins, such as UDP-arabinopyranose mutase and pectin acetylesterases as well as accumulation of proteins related to secondary metabolic defence mechanisms, such as phenylalanine ammonia lyase, 1,3,7-trihydroxyxanthone synthase, and benzophenone synthase, indicated a coordinated plant defence response. Physiological analysis also revealed significant stress responses in H. perforatum seedlings, including reduced photosynthetic performance and stomatal changes as early as 12 h, which intensified after 24 h. Taken together, the identified key proteins and altered physiological responses of H. perforatum contribute to the understanding of factors underlying recalcitrance of plants to Agrobacterium-mediated transformation.

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农杆菌诱导的贯叶连翘蛋白质组学重构及生理适应

尽管农杆菌介导的转化在植物基因工程和功能基因组学中得到了广泛的应用，但许多经济和工业上重要的作物，特别是药用植物，对农杆菌介导的T-DNA转移具有抗性，并且已被证明难以通过该技术进行改进。在这里，我们首次报道了一种重要的不可转化药用植物贯叶连翘（Hypericum perforatum）对a . tummefaciens的蛋白质组学和生理变化，采用了综合的蛋白质组学方法和生理评估。利用液相色谱/串联质谱技术对经瘤胃芽孢杆菌攻毒的穿孔叶芽孢杆菌细胞进行蛋白质组学分析，发现1200个差异表达蛋白（DEPs）；共培养24 h后共培养390个dep，其中上调174个，下调216个。功能分析显示，关键防御相关蛋白PR-10的早期激活表明病原体的快速识别和防御反应的启动。同时，抗氧化酶，如过氧化物还蛋白和l-抗坏血酸过氧化物酶显著上调，表明氧化应激的衰减强劲。细胞壁修饰蛋白的诱导，如udp -阿拉伯葡萄糖变化酶和果胶乙酰酯酶，以及与次生代谢防御机制相关的蛋白质的积累，如苯丙氨酸解氨酶、1,3,7-三羟基黄酮合成酶和二苯甲酮合成酶，表明植物的防御反应是协调一致的。生理分析还揭示了枯叶连翘幼苗对胁迫的显著响应，包括光合性能下降和气孔变化，最早在12 h， 24 h后加剧。综上所述，鉴定的关键蛋白和枯叶连翘生理反应的改变有助于理解植物对农杆菌介导转化的抗性因素。

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.