Comparative transcriptome and defense hormone analyses of resistant and susceptible tea plant varieties revealed the response mechanisms of tea plants to Apolygus lucorum feeding

IF 6.8 Q1 PLANT SCIENCES
Yuan-Hong Wan , Chun Yang , Da-He Qiao , Yue-Xin Li , Xiao-Zeng Mi , Xing-Yun Shi , Shuai Li , Jin-Feng Zhang , Yu-He Wan , Ze-Hong Meng
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引用次数: 0

Abstract

Apolygus lucorum represents a major insect pest affecting Camellia sinensis, with its feeding activity posing significant threats to tea plant growth and yield productivity. However, the molecular basis of tea plant resistance against A. lucorum infestation remains poorly characterized. In this study, we employed two distinct tea varieties, the resistant variety Taixuan 0310 (TX0310) and the susceptible variety Huangjinya (HJY), for comparative analysis. Phenotypic evaluation revealed consistently less severe damage symptoms in TX0310 compared to HJY under identical infestation conditions. Through transcriptome sequencing approaches and integrated phytohormone profiling, we systematically elucidated the differential defense responses to A. lucorum. The resistant variety TX0310 exhibited sustained and comprehensive activation of both jasmonic acid (JA) signaling cascades and secondary metabolic pathways, whereas the susceptible variety HJY displayed only transient and attenuated JA responses. Notably, key genes involved in JA biosynthesis (CsOPR11 and CsJMT) showed marked upregulation specifically in TX0310. Quantitative analysis confirmed significantly higher accumulation of defense-related phytohormones including JA and salicylic acid (SA) in TX0310 relative to HJY. Through weighted gene co-expression network analysis (WGCNA) and Pearson correlation analysis, we identified 10 core regulatory genes (including CsOPR11, CsAOS, and CsJMT). Notably, overexpression of the CsOPR11 gene in tobacco resulted in significantly higher methyl jasmonate (MeJA) levels in CsOPR11-transgenic plants compared to wild-type controls. This provides novel insights into the molecular mechanisms underlying tea plant resistance against insect pests.
抗性和易感茶树品种的转录组和防御激素对比分析揭示了茶树对绿盲蝽取食的反应机制
绿盲蝽(Apolygus lucorum)是影响茶树的主要害虫,其取食活动对茶树的生长和产量构成严重威胁。然而,茶树抗绿僵菌侵染的分子基础仍不清楚。本研究选用抗性品种太玄0310 (TX0310)和敏感品种黄金芽(HJY)两个不同的茶叶品种进行比较分析。表型评估显示,在相同的侵染条件下,TX0310的损害症状始终比HJY轻。通过转录组测序方法和整合的植物激素分析,我们系统地阐明了对绿桫椤的不同防御反应。抗性品种TX0310表现出茉莉酸信号级联和次生代谢途径的持续和全面激活,而敏感品种HJY仅表现出短暂和减弱的茉莉酸反应。值得注意的是,参与JA生物合成的关键基因(CsOPR11和CsJMT)在TX0310中特异性表达上调。定量分析证实TX0310中JA和水杨酸(SA)等防御相关植物激素的积累明显高于HJY。通过加权基因共表达网络分析(WGCNA)和Pearson相关分析,我们确定了10个核心调控基因(包括copr11、CsAOS和CsJMT)。值得注意的是,copr11基因在烟草中的过表达导致copr11转基因植株中茉莉酸甲酯(MeJA)水平显著高于野生型对照。这为茶树抗虫的分子机制提供了新的见解。
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来源期刊
Plant Stress
Plant Stress PLANT SCIENCES-
CiteScore
5.20
自引率
8.00%
发文量
76
审稿时长
63 days
期刊介绍: The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.
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