{"title":"Generational effects of biosynthesized titanium dioxide nanoparticles on bitter gourd growth and phytochemistry","authors":"Bimal Das , Bappa Paramanik , Dipak Kumar Murmu , Biman De , Partha Sarathi Patra , Prithwiraj Dey , Pradip Dey","doi":"10.1016/j.stress.2025.100985","DOIUrl":null,"url":null,"abstract":"<div><div>The biosynthesis titanium dioxide nanoparticles (TiO<sub>2</sub> NPs) on seedling growth, yield, biochemical traits, and their transmission effect over two generations were assessed in bitter gourd cv. Megna 2. Six distinct nanoscale TiO<sub>2</sub> concentrations (0, 10, 20, 50, 80, and 100 mg L<sup>-1</sup>) were applied to bitter gourd seeds, along with a control. Various characterization methods, including dynamic light scattering (DLS), UV–visible spectroscopy, fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM), were utilized to verify the dimensions, absorption spectra, functional molecules and surface structure of biosynthesis TiO<sub>2</sub> NPs. The initial study observed that the nanoparticle-treated seeds produced better germination and seedling growth (p < 0.05) than the control. Treatments with TiO<sub>2</sub> NPs at the seedling stage had a substantial effect on catalase and peroxidase activities. Parental TiO<sub>2</sub> NPs treated seeds ‘N1’ (first generation) and their progeny ‘N2’ (second generation) showed substantial changes in plant growth, fruit yield characteristics, and phytomedicine properties in bitter gourd. Concentration with 80 mg L<sup>-1</sup> over the two generations proved to be the best treatment, which significantly increased total chlorophyll content, ascorbic acid content, TSS content, beta carotene content, charantin contents, and cucurbitacin content, respectively, compared to the control. After two generations of verification of TiO<sub>2</sub> NP uptake in roots and shoots, it was discovered that the quantity of particle accumulation in the next generation had decreased but significant results achieved for growth and phytomedicinal properties in bitter gourd. These findings suggested that biosynthesized TiO<sub>2</sub> NPs can induce stable transgenerational enhancement offering promising applications in sustainable agricultural by improving both productivity and nutritional quality in edible crop plants.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"18 ","pages":"Article 100985"},"PeriodicalIF":6.8000,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Stress","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667064X25002532","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The biosynthesis titanium dioxide nanoparticles (TiO2 NPs) on seedling growth, yield, biochemical traits, and their transmission effect over two generations were assessed in bitter gourd cv. Megna 2. Six distinct nanoscale TiO2 concentrations (0, 10, 20, 50, 80, and 100 mg L-1) were applied to bitter gourd seeds, along with a control. Various characterization methods, including dynamic light scattering (DLS), UV–visible spectroscopy, fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM), were utilized to verify the dimensions, absorption spectra, functional molecules and surface structure of biosynthesis TiO2 NPs. The initial study observed that the nanoparticle-treated seeds produced better germination and seedling growth (p < 0.05) than the control. Treatments with TiO2 NPs at the seedling stage had a substantial effect on catalase and peroxidase activities. Parental TiO2 NPs treated seeds ‘N1’ (first generation) and their progeny ‘N2’ (second generation) showed substantial changes in plant growth, fruit yield characteristics, and phytomedicine properties in bitter gourd. Concentration with 80 mg L-1 over the two generations proved to be the best treatment, which significantly increased total chlorophyll content, ascorbic acid content, TSS content, beta carotene content, charantin contents, and cucurbitacin content, respectively, compared to the control. After two generations of verification of TiO2 NP uptake in roots and shoots, it was discovered that the quantity of particle accumulation in the next generation had decreased but significant results achieved for growth and phytomedicinal properties in bitter gourd. These findings suggested that biosynthesized TiO2 NPs can induce stable transgenerational enhancement offering promising applications in sustainable agricultural by improving both productivity and nutritional quality in edible crop plants.
期刊介绍:
The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues.
Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and:
Lack of water (drought) and excess (flooding),
Salinity stress,
Elevated temperature and/or low temperature (chilling and freezing),
Hypoxia and/or anoxia,
Mineral nutrient excess and/or deficiency,
Heavy metals and/or metalloids,
Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection,
Viral, phytoplasma, bacterial and fungal plant-pathogen interactions.
The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.