Plant Stress最新文献

{"title":"Exogenous EBR enhanced heat tolerance in mini Chinese cabbage by regulating ABA accumulation","authors":"Jiaojiao Yang ,&nbsp;Guangzheng Wang ,&nbsp;Yongmei He ,&nbsp;Wenxu Chen ,&nbsp;Xuehua Wang ,&nbsp;Jizhong Ma ,&nbsp;Xueqing Gao ,&nbsp;Jihua Yu ,&nbsp;Linli Hu","doi":"10.1016/j.stress.2025.100784","DOIUrl":"10.1016/j.stress.2025.100784","url":null,"abstract":"<div><div>It is unclear whether 24-epibrassinolide (EBR) can improve the heat resistance of mini Chinese cabbage (<em>Brassica pekinensis</em>). Whether abscisic acid (ABA) can be involved in EBR to improve the heat tolerance of mini Chinese cabbage is not clear. Therefore, in this study, mini Chinese cabbage was used as experimental material, and the effects of EBR/ABA and its synthetic inhibitors were studied by applying pharmacological methods to explore the effects of EBR and ABA on improving the heat resistance of mini Chinese cabbage. Our experimental results revealed that EBR pretreatment can reduce reactive oxygen species (ROS) under heat stress, protect the photosynthetic system, and maintain stomatal function, improving the heat resistance of mini Chinese cabbage. It is noteworthy that the application of fluriddone (FDT) or brassinazole (Brz) to EBR or ABA treated mini Chinese cabbage significantly reduced the stability of the membrane system and photosynthetic system. Based on these observations, we speculated that EBR and ABA may have a synergistic effect to jointly promote the heat resistance of mini Chinese cabbage. Transcriptome result showed that EBR pretreatment significantly increased the relative expression levels of <em>BrNCED3</em> and <em>BrPYL</em> genes, and decreased the relative expression levels of <em>BrCYP707A1</em> genes. These results suggested that EBR activates downstream signaling pathways by promoting ABA accumulation under heat stress. In conclusion, exogenous EBR can improve the heat resistance of mini Chinese cabbage by regulating ABA accumulation. This study provides new insights into the potential mechanism of EBR alleviating heat stress in mini Chinese cabbage.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100784"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of secondary metabolism in Cannabis sativa glandular trichomes by abscisic acid and water deficit stress during late flowering development","authors":"Nicolas Dimopoulos ,&nbsp;Qi Guo ,&nbsp;Lei Liu ,&nbsp;Razlin Azman Halimi ,&nbsp;Lennard Garcia-de Heer ,&nbsp;Matthew Nolan ,&nbsp;Jos C. Mieog ,&nbsp;Bronwyn J. Barkla ,&nbsp;Tobias Kretzschmar","doi":"10.1016/j.stress.2025.100799","DOIUrl":"10.1016/j.stress.2025.100799","url":null,"abstract":"<div><div>Cannabis (<em>Cannabis sativa</em> L.) is a medicinally important plant of moderate drought tolerance. The glandular trichomes (GT) on female flowers of cannabis are the main site of production and storage of cannabinoids and terpenoids. While water deficit stress (WD) was shown to modulate cannabinoid content in cannabis, it remains unknown how GT regulation is affected to achieve this. We quantified the GT proteome and floral cannabinoid and terpenoid content in late flowering ‘Hindu Kush’ cannabis treated with either short-term WD or with an abscisic acid (ABA) foliar spray. We found that WD and ABA changed the abundance of many of the same proteins in the GT, with changes reflecting a decrease in primary metabolic processes and an increase in stress response mechanisms. Results suggested ABA likely mediates changes in the GT indirectly by affecting salicylic acid and ethylene signalling. WD caused the cannabinoid cannabigerolic acid to increase in content, while ABA caused monoterpene content to increase. Interestingly, proteins involved in the cannabinoid and terpenoid biosynthetic pathways either decreased in abundance or did not change in either treatment, indicating that the increased secondary metabolite content is likely driven by a process other than upregulation of the pathways’ proteins.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100799"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potato cultivars use distinct mechanisms for salt stress acclimation","authors":"Michael Nicolas ,&nbsp;Jort Bouma ,&nbsp;Jan Henk Venema ,&nbsp;Hanneke van der Schoot ,&nbsp;Francel Verstappen ,&nbsp;Thijs de Zeeuw ,&nbsp;Sanne E. Langedijk ,&nbsp;Damian Boer ,&nbsp;Johan Bucher ,&nbsp;Marten Staal ,&nbsp;Ben Krom ,&nbsp;J. Theo M Elzenga ,&nbsp;Richard G.F. Visser ,&nbsp;Christa Testerink ,&nbsp;Rumyana Karlova","doi":"10.1016/j.stress.2025.100798","DOIUrl":"10.1016/j.stress.2025.100798","url":null,"abstract":"<div><div>Soil salinity induces osmotic stress and ion toxicity in plants, detrimentally affecting their growth. Potato (<em>Solanum tuberosum</em>) suffers yield reductions under salt stress. To understand salt-stress resilience mechanisms in potatoes, we studied three cultivars with contrasting salt sensitivity: Innovator, Desirée, and Mozart. Innovator emerged as the most resilient under salt stress, displaying minimal reductions in growth and plant tolerance index with no tuber yield loss, despite notable water loss. Conversely, Desirée experienced a significant tuber yield reduction but maintained better water retention. Mozart showed a low plant tolerance index and high water loss. Interestingly, ions measurement across different tissues revealed that, unlike chloride, sodium does not accumulate in tubers under salt stress in these cultivars, suggesting existence of an active sodium exclusion mechanism. A whole root transcriptomic analysis of these cultivars revealed a conserved salt stress response between potato and Arabidopsis<em>.</em> This response includes activation of various abiotic stress pathways and involves sequential activation of various transcription factor families. Root analyses showed that Innovator has lower suberin and lignin deposition, along with stronger K⁺ leakage in control conditions, resulting in a higher early stress response and increased ABA accumulation shortly after salt stress induction. This could explain Innovator has a more divergent transcriptomic response to salt stress compared to Desirée and Mozart. Nevertheless, Innovator displayed high suberin and lignin levels and ceased K⁺ leakage after salt stress, suggesting a high acclimation ability. Altogether, our results indicate that acclimation ability, rather than initial root protection against salt prevails in long-term salt-stress resilience of potato.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100798"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chloroplast positioning affects Hexokinase 1-mediated plant immunity under combined low temperature and high light","authors":"Sophia Bianca Bagshaw ,&nbsp;Anastasia Kitashova ,&nbsp;Beyza Özmen ,&nbsp;Chun Kwan Yip ,&nbsp;Bianca Emily Süling ,&nbsp;Laura Schröder ,&nbsp;Tatjana Kleine ,&nbsp;Thomas Nägele","doi":"10.1016/j.stress.2025.100791","DOIUrl":"10.1016/j.stress.2025.100791","url":null,"abstract":"<div><div>Molecular networks involved in the responses of plants towards environmental changes are multifaceted and affect diverse metabolic and signalling pathways. Under challenging environmental conditions, such as low temperatures and high light intensities, plants need to immediately adjust their metabolism to prevent irreversible tissue damage. Regulation of photosynthesis and carbohydrate metabolism plays a crucial role in this stress response. Here, we analysed mutants of <em>Arabidopsis thaliana</em>, which were affected in either central enzymatic activities of carbohydrate metabolism, in chloroplast positioning or in a combination of both. Plants were exposed to a treatment of combined cold and elevated light. While mutants with deficiencies in sucrose or starch metabolism showed affected metabolic pathway regulation under abiotic stress, Hexokinase 1 mutants (<em>hxk1</em>) showed a severe growth phenotype with lesions and pale areas on leaf tissue. In a double mutant, combining deficiencies in Chloroplast Unusual Positioning 1 (CHUP1)-mediated chloroplast positioning and HXK1 (<em>hxk1 x chup1</em>), this growth phenotype vanished resulting in wild type-like plants. Transcriptome analysis revealed a significantly affected immune response of <em>hxk1</em> plants, which was suppressed in the double mutants. Our results support previous findings which suggested that HXK1 acts as a positive regulator of the plant immune response. Finally, we suggest that, due to its potential role as a negative regulator of plant immunity, CHUP1 deficiency counteracted the reduced immunity of <em>hxk1</em> in the double mutant which rescued the plants. Future studies might now reveal whether deficiencies in CHUP1 function and/or transcription represent a conserved strategy to increase plant immunity under abiotic stress.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100791"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium-induced avoidance mechanism, ionic interactions, and antioxidant system to mitigate manganese toxicity in apple rootstock 'Qingzhen 1′","authors":"Zhiyuan Li,&nbsp;Wanying Xie,&nbsp;Yu Tian,&nbsp;Jie Shen,&nbsp;Xintong Su,&nbsp;Jingjing Yang,&nbsp;Meixia Liang,&nbsp;Xuqiang Qiao","doi":"10.1016/j.stress.2025.100797","DOIUrl":"10.1016/j.stress.2025.100797","url":null,"abstract":"<div><div>Manganese toxicity poses a significant challenge to apple tree growth. Although selenium has been shown to mitigate heavy metal toxicity, the underlying mechanisms by which Se reduces Mn accumulation and toxicity remain poorly understood, particularly in the apple rootstock ‘<em>Qingzhen 1</em>′. This study investigates the role of Se in Mn detoxification, focusing on avoidance mechanisms (such as amino acid and organic acid regulation, phytochelatin and metallothionein expression), ion competition, and modulation of the antioxidant defense system. The application of selenium not only reduced the Mn content by 14.64 % but also improved key growth parameters compared to the Mn only treatment. The amino acid level was increased by 2.86 %-32.55 %, the concentration of citric acid and tartaric acid was increased by 9.01 % and 47.30 %, the activity and expression of glutathione synthase and plant chelating protein synthase were increased, and the expression of metallothionein was regulated. Furthermore, Se facilitated the uptake of essential nutrients, including Ca, Zn, Cu, and S, which helped restore the plant's nutritional balance. Additionally, Se bolstered the plant's oxidative stress tolerance by enhancing the activities of catalase and superoxide dismutase, while increasing levels of ascorbic acid, glutathione, and non-protein sulphhydryls. Consequently, less hydrogen peroxide and nitric oxide accumulation was observed. Overall, selenium mitigated Mn toxicity in apple rootstock ‘<em>Qingzhen 1</em>′ by activating avoidance strategies, optimizing ion competition, and enhancing antioxidant responses. These findings provide new insights into how Se can reduce Mn uptake and alleviate Mn-induced toxicity in plants.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100797"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional analyses revealed Pinus radiata CHLOROPLAST SENSOR KINASE as a key component in UV-B stress and development","authors":"Lara García-Campa ,&nbsp;Jesús Pascual ,&nbsp;Mónica Meijón ,&nbsp;Sara Guerrero ,&nbsp;David Morales ,&nbsp;José M. Álvarez ,&nbsp;Luis Valledor","doi":"10.1016/j.stress.2025.100796","DOIUrl":"10.1016/j.stress.2025.100796","url":null,"abstract":"<div><div>Deciphering pine response mechanisms to UV-B stress enhanced by climate change is crucial to establish damage-reducing strategies and avoid losses for forestry sector. In this study, <em>CHLOROPLAST SENSOR KINASE</em> from <em>Pinus radiata</em> (<em>PrCSK</em>), previously identified as a potentially relevant component in UV-B stress response using a systems biology approach, was sequenced and used to generate overexpression and RNAi <em>Arabidopsis thaliana</em> lines to carry out functional validation studies employing phenotypical, physiological, and metabolomic analyses. Results demonstrated CSK evolutionary conservation between <em>A. thaliana</em> and <em>P. radiata</em> and its involvement in growth, development, and UV-B response and tolerance. Notably, overexpression lines showed enhanced growth, photosynthetic performance, and stress resilience linked to efficient water and carbon use, as well as resilient and efficient carbohydrate metabolism. Conversely, amino acid metabolism was essential for plant recovery when cellular damage increased due to <em>CSK</em> down-regulation, which also resulted in severe growth retardation. Moreover, secondary metabolism, represented by the accumulation of phenolic compounds, was also modified according to <em>CSK</em> expression levels. Overall, these results propose <em>PrCSK</em> as a valuable biomarker to select tolerant trees to adverse UV-B environmental conditions expected in the coming years, and to increase pine biomass and production to meet current and future global demand of timber products.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100796"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of MRE11 from the moss Physcomitrium patens enhances resistance to genotoxic DNA strand breaks by stimulating homologous recombination","authors":"Arup Das,&nbsp;Sudipta Ray","doi":"10.1016/j.stress.2025.100786","DOIUrl":"10.1016/j.stress.2025.100786","url":null,"abstract":"<div><div>Homology-directed repair (HDR) plays a crucial role in maintaining genetic integrity by facilitating error-free repair of DNA double-strand breaks (DSBs). The meiotic recombination 11 (MRE11) nuclease, the structural center of the MRE11-RAD50-NBS1 (MRN) complex, critically senses DSBs and initiates DNA end resection to commence the HDR process. Here, we report the functional role of MRE11 from the moss <em>Physcomitrium patens</em> (PpMRE11) in enhancing DNA damage tolerance. Our results demonstrate that the overexpression of PpMRE11 confers resistance to genotoxic DSBs in both bacterial and plant systems. Quantitative real-time analysis of the <em>PpMRE11</em> transcript revealed elevated expression in response to diverse stress stimuli. Furthermore, the increased survival in response to genotoxic stress facilitated by PpMRE11 overexpression is directly linked to the higher homologous recombination (HR) frequency - a correlation not observed in bacterial mutant lines lacking HR capability. Moreover, ectopic expression of PpMRE11 in transgenic rice plants improved resistance to genotoxic stress, as evidenced by reduced accumulation of DNA lesions and decreased cellular damage. These findings provide compelling evidence that the overexpression of PpMRE11 promotes HDR and enhances DNA damage tolerance, highlighting its potential as a target for genetic engineering strategies aimed at improving stress resistance in crops.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100786"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of photosystem integrity and reducing Cd accumulation in mitigating Cd stress on olive tree inoculated with soil-borne bacteria consortium is variety-dependent","authors":"Rabaa Hidri ,&nbsp;Rahma Azri ,&nbsp;Katja Burow ,&nbsp;Walid Zorrig ,&nbsp;Ahmed Debez ,&nbsp;Nouf M. Alyami ,&nbsp;Mokded Rabhi ,&nbsp;Asma Bensalem-Fnayou ,&nbsp;Ahmed Mliki ,&nbsp;Philipp Franken ,&nbsp;Ricardo Aroca","doi":"10.1016/j.stress.2025.100788","DOIUrl":"10.1016/j.stress.2025.100788","url":null,"abstract":"<div><div>Rhizosphere microbial community role in boosting fruit tree ability to cope with heavy metals is gaining interest. Here, we address the effects of in-frame holobiont microbial inoculation on two Tunisian olive tree varieties (<em>Olea europaea</em> L. cv. Chemlali and Chetoui) exposed to CdCl₂ for one month. Shoot Cd content, leaf pigment contents, chlorophyll fluorescence, and energy conversion-related traits in photosystems I and II (PSI and PSII) were considered. Following pyrosequencing-based 16S rRNA analysis, Proteobacteria, and to a lesser extent Firmicutes phylums, were the most abundant. Both Cd-treated olive varieties showed a significant decrease in leaf pigment contents, however, this depressive effect was significantly mitigated following inoculation. PSI and PSII activities were more altered in Cd-treated Chetoui as compared to Chemlali. Quantum yield and electron transport of PSI and PSII were also more impacted in Chetoui, whereas the limitation of the donor side of PSI increased in the latter. Yet, Cd impact on these parameters was significantly reduced in inoculated plants. The quantum yield of nonregulated non-photochemical energy loss in PSII significantly increased in both olive varieties under Cd stress, but values were markedly restored with inoculation. High Cd accumulation observed in Chetoui shoots upon Cd exposure was significantly lower in inoculated plants. Overall, the consortium used improved olive tree Cd tolerance likely due to the restoration of leaf pigment contents and the better integrity of photosystems in both varieties, whereas lowering Cd translocation to shoots would be more prominent in Chetoui. Long-term experiments will be conducted to confirm these results.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100788"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing saline-alkali tolerance in cucumber seedlings: The role of exogenous melatonin in redox homeostasis and stomatal function","authors":"Shuchao Huang ,&nbsp;Peng Wu ,&nbsp;Xiting Yang ,&nbsp;Wei Li ,&nbsp;Wenhao Zhou ,&nbsp;Yandong Xie ,&nbsp;Xin Meng ,&nbsp;Zhaozhuang Li ,&nbsp;Zhiqi Xu ,&nbsp;Ning Jin ,&nbsp;Li Jin ,&nbsp;Shuya Wang ,&nbsp;Jian Lyu ,&nbsp;Jihua Yu","doi":"10.1016/j.stress.2025.100789","DOIUrl":"10.1016/j.stress.2025.100789","url":null,"abstract":"<div><div>Cucumbers, cultivated globally on 3.7 million hectares, face yield losses due to salinity, highlighting the need for effective mitigation strategies for degraded soils. Melatonin (MT) has gained significant interest for its ability to relieve plant stress. To explore the regulatory role of exogenous MT in maintaining redox homeostasis in cucumber seedlings under saline-alkali stress (SA), this study employed the cucumber cultivar 'Xinchun No. 4′. Simulated saline-alkali conditions were applied, and the effects of exogenous MT on seedling growth, reactive oxygen species (ROS) production, the ascorbate-glutathione (AsA-GSH) cycle, and changes in leaf anatomy were systematically assessed. The findings reveal that exposure to 40 mmol·L^-1 saline-alkali stress significantly impaired cucumber seedling growth, reduced biomass, and led to excessive accumulation of hydrogen peroxide (H₂O₂) and superoxide anions (O₂·⁻) in the leaves. This, resulted in increased lipid peroxidation (indicated by elevated malondialdehyde (MDA) levels), whichi further compromised the cell membrane. Application of 10 μmol·L^-1 MT effectively reduced ROS levels, lowered MDA content, and mitigated electrolyte leakage. MT also enhanced AsA and GSH levels, improved AsA/DHA and GSH/GSSG ratios, and upregulated key AsA-GSH cycle genes (<em>CsAPX, CsAAO, CsMDAR, CsDHAR, CsGR</em>), leading to a significant increase in enzymatic activity. In addition, MT alleviated stress-induced stomatal closure, thereby restoring normal stomatal function. These findings suggest that MT enhances saline-alkali tolerance by mitigating oxidative damage, promoting antioxidant defenses, and effectively preserving stomatal function. Thus, our study points to a sustainable strategy to improve crop resilience in salinized environments via MT application.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100789"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal community dynamics and anthocyanin profiling of grapevine leaves in a vineyard affected by esca","authors":"Giovanni Del Frari ,&nbsp;Chiara Ingrà ,&nbsp;Marie Rønne Aggerbeck ,&nbsp;Alex Gobbi ,&nbsp;Teresa Nascimento ,&nbsp;Ana Cabral ,&nbsp;Helena Oliveira ,&nbsp;Lars Hestbjerg Hansen ,&nbsp;Alessandra Ferrandino ,&nbsp;Ricardo Boavida Ferreira","doi":"10.1016/j.stress.2025.100793","DOIUrl":"10.1016/j.stress.2025.100793","url":null,"abstract":"<div><div>Leaves in grapevines affected by esca, a grapevine trunk disease, may occasionally manifest a symptom known as the ‘esca leaf stripe symptom.’ Most frequently, this symptom appears as scorching in the interveinal tissue, with red pigmentation observed between scorched and healthy tissue. However, purple pigmentation or an absence of pigmentation is occasionally reported. The synthesis and accumulation of anthocyanins may drive these different symptom phenotypes. Recent evidence also implicates fungal endophytes in host manipulation, potentially influencing grapevine metabolic profiles, including anthocyanins. In this study, working on cultivars Cabernet Sauvignon and Touriga Nacional, we used DNA metabarcoding (i) to explore the microbial dynamics of endophytes during symptom progression in esca-affected leaves, and (ii) to reveal the fungal diversity for different symptom phenotypes, along with their qualitative and quantitative anthocyanins composition.</div><div>The endophytic mycobiome profiling revealed a large fungal richness (260 taxa), and a beta diversity influenced by cultivar (<em>P</em> &lt; 0.01) and vintage (<em>P</em> = 0.001). We observed significant differences in beta diversity between leaves affected by chlorotic spots and asymptomatic ones (<em>P</em> &lt; 0.05), revealing major shifts in fungal community composition during early stages of esca symptom progression. Comparing asymptomatic leaves and different symptom phenotypes, we detected cultivar and vintage-dependent alterations in alpha and beta diversity, as well as in individual taxa abundance (e.g. <em>Botrytis caroliniana</em> over-represented in red leaves). Total anthocyanin accumulation was influenced by cultivar (<em>P</em> ≤ 0.0001) but not by vintage. In Touriga Nacional, and to a lesser extent in Cabernet Sauvignon, purple leaves accumulated significantly lower amounts of tri-hydroxylated anthocyanins and acyl-derivatives, when compared to red leaves.</div><div>Fungal communities significantly alter in composition during esca symptom progression, and for different symptom phenotypes, suggesting a strong correlation between microbial structure and the physiological and biochemical processes that occur in leaves.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"15 ","pages":"Article 100793"},"PeriodicalIF":6.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143563858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}