核转录因子ZmCCT正调控玉米对盐胁迫和低氮胁迫的响应

IF 6.8 Q1 PLANT SCIENCES
Yanbing Zhang , Zhihuan Zhou , Senlin Xiao , Yipu Li , Suxiao Hao , Fan Que , Zhongjia Liu , Liyu Shi , Yingying Shi , Zhaoheng Zhang , Yang Xu , Tonghui Li , Yaxing Shi , Chun Yin , Wei Song , Ronghuan Wang , Weixiang Wang
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引用次数: 0

摘要

干旱、盐度和低氮等非生物胁迫对玉米生长发育产生负面影响,导致产量显著下降。在之前的研究中,我们成功克隆了玉米转录因子基因ZmCCT,并通过光循环途径证明了其在开花调控中的作用。此外,我们发现ZmCCT启动子区域的转座因子(TE)插入降低了玉米对茎腐病的抗性。然而,尽管ZmCCT在多年前就被克隆出来,但其响应生物和非生物胁迫的关键分子机制仍不清楚。在本研究中,我们利用Y331/Y331-ΔTE自交系和83B28H1/H1/83B28H5/H5单倍型,证明了ZmCCT在玉米耐盐和低氮胁迫中发挥重要作用。通过DAB染色和H2O2含量分析,我们证实Y331-ΔTE和83B28H5/H5在高盐低氮处理后,膜系统损伤较小,耐应力能力较强。在高盐低氮胁迫条件下,Y331-ΔTE和83B28H5/H5自交系的表型表现优于Y331和83B28H1/H1。此外,与野生型植物相比,过表达ZmCCT的转基因拟南芥对盐胁迫和低氮胁迫的耐受性增强。此外,RNA-Seq分析表明,ZmCCT在对高盐低氮耐受做出反应时,可以直接激活ZmNADP、ZmPP2C、ZmbHLH55、ZmPIP1-1、ZmPIP2-4等盐诱导基因以及ZmWRKY47、ZmMYB44、ZmMYB36、ZmPIN10和ZmbHLH83等低氮相关基因。综上所述,我们的研究结果表明ZmCCT在高盐低氮胁迫耐受中发挥了重要作用,并进一步强调了ZmCCT具有多种非生物胁迫作用。这些结果表明,ZmCCT可能是玉米分子设计育种中提高植物盐和低氮胁迫的潜在候选基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The nuclear transcription factor ZmCCT positively regulates salt and low nitrogen stress response in Maize
Abiotic stresses such as drought, salinity, and low nitrogen negatively affect maize growth and development, leading to significant yield reductions. In previous studies, we successfully cloned the maize transcription factor gene ZmCCT and demonstrated its role in flowering regulation through the photocycle pathway. Additionally, we found that transposable element (TE) insertions in the ZmCCT promoter region reduce maize resistance to stem rot. However, although ZmCCT was cloned years ago, its key molecular mechanisms in response to biotic and abiotic stresses remain unclear. In this study, we demonstrated that ZmCCT plays important roles in salt and low-nitrogen stress tolerance in maize, using the Y331/Y331-ΔTE inbred line and 83B28H1/H1/83B28H5/H5 haplotypes. Through DAB staining and H2O2 content analysis, we confirmed that Y331-ΔTE and 83B28H5/H5 exhibited less membrane system damage and greater stress tolerance following high-salt and low-nitrogen treatments. Under high salt and low nitrogen stress conditions, the Y331-ΔTE and 83B28H5/H5 inbred lines demonstrated superior phenotypic performance compared to the Y331 and 83B28H1/H1 lines. Furthermore, transgenic Arabidopsis thaliana overexpressing ZmCCT showed enhanced tolerance to salt and low nitrogen stress compared with wild-type plants. In addition, RNA-Seq analysis indicated that ZmCCT can directly activate these salt inducible genes of ZmNADP, ZmPP2C, ZmbHLH55, ZmPIP1–1, ZmPIP2–4 and some low nitrogen involved genes of ZmWRKY47, ZmMYB44, ZmMYB36, ZmPIN10 and ZmbHLH83 when respond to high salt and low nitrogen tolerance. Taken together, our results have provided that ZmCCT functions as important roles in high salt and low nitrogen stress tolerance and further highlight that ZmCCT has multiple abiotic stress roles. These results indicate that ZmCCT may be a potential candidate to enhance plant salt and low nitrogen stresses in mazie molecular design breeding.
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来源期刊
Plant Stress
Plant Stress PLANT SCIENCES-
CiteScore
5.20
自引率
8.00%
发文量
76
审稿时长
63 days
期刊介绍: The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.
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