Yanbing Zhang , Zhihuan Zhou , Senlin Xiao , Yipu Li , Suxiao Hao , Fan Que , Zhongjia Liu , Liyu Shi , Yingying Shi , Zhaoheng Zhang , Yang Xu , Tonghui Li , Yaxing Shi , Chun Yin , Wei Song , Ronghuan Wang , Weixiang Wang
{"title":"核转录因子ZmCCT正调控玉米对盐胁迫和低氮胁迫的响应","authors":"Yanbing Zhang , Zhihuan Zhou , Senlin Xiao , Yipu Li , Suxiao Hao , Fan Que , Zhongjia Liu , Liyu Shi , Yingying Shi , Zhaoheng Zhang , Yang Xu , Tonghui Li , Yaxing Shi , Chun Yin , Wei Song , Ronghuan Wang , Weixiang Wang","doi":"10.1016/j.stress.2025.100893","DOIUrl":null,"url":null,"abstract":"<div><div>Abiotic stresses such as drought, salinity, and low nitrogen negatively affect maize growth and development, leading to significant yield reductions. In previous studies, we successfully cloned the maize transcription factor gene <em>ZmCCT</em> and demonstrated its role in flowering regulation through the photocycle pathway. Additionally, we found that transposable element (TE) insertions in the <em>ZmCCT</em> promoter region reduce maize resistance to stem rot. However, although <em>ZmCCT</em> was cloned years ago, its key molecular mechanisms in response to biotic and abiotic stresses remain unclear. In this study, we demonstrated that <em>ZmCCT</em> plays important roles in salt and low-nitrogen stress tolerance in maize, using the Y331/Y331-ΔTE inbred line and 83B28<em><sup>H1</sup><sup>/H1</sup>/</em>83B28<em><sup>H5</sup><sup>/H5</sup></em> haplotypes. Through DAB staining and H<sub>2</sub>O<sub>2</sub> content analysis, we confirmed that Y331-ΔTE and 83B28<em><sup>H5/H5</sup></em> exhibited less membrane system damage and greater stress tolerance following high-salt and low-nitrogen treatments. Under high salt and low nitrogen stress conditions, the Y331-ΔTE and 83B28<em><sup>H5</sup><sup>/H5</sup></em> inbred lines demonstrated superior phenotypic performance compared to the Y331 and 83B28<em><sup>H1</sup><sup>/H1</sup></em> lines. Furthermore, transgenic <em>Arabidopsis thaliana</em> overexpressing <em>ZmCCT</em> showed enhanced tolerance to salt and low nitrogen stress compared with wild-type plants. In addition, RNA-Seq analysis indicated that <em>ZmCCT</em> can directly activate these salt inducible genes of <em>ZmNADP, ZmPP2C, ZmbHLH55, ZmPIP1–1, ZmPIP2–4</em> and some low nitrogen involved genes of <em>ZmWRKY47, ZmMYB44, ZmMYB36, ZmPIN10</em> and <em>ZmbHLH83</em> when respond to high salt and low nitrogen tolerance. Taken together, our results have provided that <em>ZmCCT</em> functions as important roles in high salt and low nitrogen stress tolerance and further highlight that <em>ZmCCT</em> has multiple abiotic stress roles. These results indicate that <em>ZmCCT</em> may be a potential candidate to enhance plant salt and low nitrogen stresses in mazie molecular design breeding.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"16 ","pages":"Article 100893"},"PeriodicalIF":6.8000,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The nuclear transcription factor ZmCCT positively regulates salt and low nitrogen stress response in Maize\",\"authors\":\"Yanbing Zhang , Zhihuan Zhou , Senlin Xiao , Yipu Li , Suxiao Hao , Fan Que , Zhongjia Liu , Liyu Shi , Yingying Shi , Zhaoheng Zhang , Yang Xu , Tonghui Li , Yaxing Shi , Chun Yin , Wei Song , Ronghuan Wang , Weixiang Wang\",\"doi\":\"10.1016/j.stress.2025.100893\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Abiotic stresses such as drought, salinity, and low nitrogen negatively affect maize growth and development, leading to significant yield reductions. In previous studies, we successfully cloned the maize transcription factor gene <em>ZmCCT</em> and demonstrated its role in flowering regulation through the photocycle pathway. Additionally, we found that transposable element (TE) insertions in the <em>ZmCCT</em> promoter region reduce maize resistance to stem rot. However, although <em>ZmCCT</em> was cloned years ago, its key molecular mechanisms in response to biotic and abiotic stresses remain unclear. In this study, we demonstrated that <em>ZmCCT</em> plays important roles in salt and low-nitrogen stress tolerance in maize, using the Y331/Y331-ΔTE inbred line and 83B28<em><sup>H1</sup><sup>/H1</sup>/</em>83B28<em><sup>H5</sup><sup>/H5</sup></em> haplotypes. Through DAB staining and H<sub>2</sub>O<sub>2</sub> content analysis, we confirmed that Y331-ΔTE and 83B28<em><sup>H5/H5</sup></em> exhibited less membrane system damage and greater stress tolerance following high-salt and low-nitrogen treatments. Under high salt and low nitrogen stress conditions, the Y331-ΔTE and 83B28<em><sup>H5</sup><sup>/H5</sup></em> inbred lines demonstrated superior phenotypic performance compared to the Y331 and 83B28<em><sup>H1</sup><sup>/H1</sup></em> lines. Furthermore, transgenic <em>Arabidopsis thaliana</em> overexpressing <em>ZmCCT</em> showed enhanced tolerance to salt and low nitrogen stress compared with wild-type plants. In addition, RNA-Seq analysis indicated that <em>ZmCCT</em> can directly activate these salt inducible genes of <em>ZmNADP, ZmPP2C, ZmbHLH55, ZmPIP1–1, ZmPIP2–4</em> and some low nitrogen involved genes of <em>ZmWRKY47, ZmMYB44, ZmMYB36, ZmPIN10</em> and <em>ZmbHLH83</em> when respond to high salt and low nitrogen tolerance. Taken together, our results have provided that <em>ZmCCT</em> functions as important roles in high salt and low nitrogen stress tolerance and further highlight that <em>ZmCCT</em> has multiple abiotic stress roles. These results indicate that <em>ZmCCT</em> may be a potential candidate to enhance plant salt and low nitrogen stresses in mazie molecular design breeding.</div></div>\",\"PeriodicalId\":34736,\"journal\":{\"name\":\"Plant Stress\",\"volume\":\"16 \",\"pages\":\"Article 100893\"},\"PeriodicalIF\":6.8000,\"publicationDate\":\"2025-05-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Stress\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667064X25001617\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Stress","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667064X25001617","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
The nuclear transcription factor ZmCCT positively regulates salt and low nitrogen stress response in Maize
Abiotic stresses such as drought, salinity, and low nitrogen negatively affect maize growth and development, leading to significant yield reductions. In previous studies, we successfully cloned the maize transcription factor gene ZmCCT and demonstrated its role in flowering regulation through the photocycle pathway. Additionally, we found that transposable element (TE) insertions in the ZmCCT promoter region reduce maize resistance to stem rot. However, although ZmCCT was cloned years ago, its key molecular mechanisms in response to biotic and abiotic stresses remain unclear. In this study, we demonstrated that ZmCCT plays important roles in salt and low-nitrogen stress tolerance in maize, using the Y331/Y331-ΔTE inbred line and 83B28H1/H1/83B28H5/H5 haplotypes. Through DAB staining and H2O2 content analysis, we confirmed that Y331-ΔTE and 83B28H5/H5 exhibited less membrane system damage and greater stress tolerance following high-salt and low-nitrogen treatments. Under high salt and low nitrogen stress conditions, the Y331-ΔTE and 83B28H5/H5 inbred lines demonstrated superior phenotypic performance compared to the Y331 and 83B28H1/H1 lines. Furthermore, transgenic Arabidopsis thaliana overexpressing ZmCCT showed enhanced tolerance to salt and low nitrogen stress compared with wild-type plants. In addition, RNA-Seq analysis indicated that ZmCCT can directly activate these salt inducible genes of ZmNADP, ZmPP2C, ZmbHLH55, ZmPIP1–1, ZmPIP2–4 and some low nitrogen involved genes of ZmWRKY47, ZmMYB44, ZmMYB36, ZmPIN10 and ZmbHLH83 when respond to high salt and low nitrogen tolerance. Taken together, our results have provided that ZmCCT functions as important roles in high salt and low nitrogen stress tolerance and further highlight that ZmCCT has multiple abiotic stress roles. These results indicate that ZmCCT may be a potential candidate to enhance plant salt and low nitrogen stresses in mazie molecular design breeding.
期刊介绍:
The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues.
Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and:
Lack of water (drought) and excess (flooding),
Salinity stress,
Elevated temperature and/or low temperature (chilling and freezing),
Hypoxia and/or anoxia,
Mineral nutrient excess and/or deficiency,
Heavy metals and/or metalloids,
Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection,
Viral, phytoplasma, bacterial and fungal plant-pathogen interactions.
The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.