Suitable light intensity stimulated polysaccharide biosynthesis in bletilla striata pseudobulbs through regulating starch and sucrose metabolism

IF 6.8 Q1 PLANT SCIENCES
Jiao Zhu , Liuyan Yang , Youming Cai , Xinhua Zeng , Yongchun Zhang , Weichang Huang
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引用次数: 0

Abstract

Bletilla striata is a famous medicinal plant, which medicinal function on polysaccharide. However, the effect of light intensity on polysaccharide accumulation in B. striata pseudobulbs is largely unknown. B. striata plants were exposed to three different light intensities: low light (5–20 μmol·m−2·s−1; LP), middle light (200 μmol·m−2·s−1; MP), and high light (400 μmol·m−2·s−1; HP). The results indicated that polysaccharide content of new pseudobulbs significantly increased under MP, and enrichment in starch and sucrose metabolism with metabolomics and transcriptomics. More sucrose was produced in the leaves through photosynthesis and was efficiently distributed to the pseudobulbs, where it was hydrolyzed into key metabolites for polysaccharide biosynthesis function by SUS and CSLA genes. The key metabolites in the polysaccharide biosynthetic pathway, such as sucrose-6-phosphate, fructose, fructose-6-phosphate, glucose-6-phosphate, GDP-mannose, and uridine 5′-diphospho-d-glucose, were upregulated under MP. Furthermore, the expression of the UGP2 gene involved in polysaccharide biosynthesis was significantly higher under MP than under LP and HP. Conversely, sucrose in leaves was minimally transported into pseudobulbs due to insufficient photosynthesis under LP and energy metabolism related to carbohydrate degradation and oxidation was hindered under HP. Thus, suitable light intensity effectively stimulated polysaccharide formation in B. striata pseudobulbs through starch and sucrose metabolism–mediated regulation.
适宜的光照强度通过调节淀粉和蔗糖代谢来促进白芨假球茎中多糖的合成
白芨是一种著名的药用植物,其药用功能是多糖。然而,光照强度对白芨假球茎中多糖积累的影响在很大程度上是未知的。将纹状体植物暴露在弱光(5 ~ 20 μmol·m−2·s−1,LP)、中光(200 μmol·m−2·s−1,MP)和强光(400 μmol·m−2·s−1,HP)下。结果表明,MP处理显著提高了新假球茎的多糖含量,并通过代谢组学和转录组学分析提高了淀粉和蔗糖代谢。叶片通过光合作用产生了更多的蔗糖,并有效地分配到假鳞茎中,在假鳞茎中被SUS和CSLA基因水解成多糖生物合成功能的关键代谢物。多糖生物合成途径中的关键代谢物,如蔗糖-6-磷酸、果糖、果糖-6-磷酸、葡萄糖-6-磷酸、甘露糖和尿苷5′-二磷酸-葡萄糖在MP作用下上调。此外,与多糖生物合成相关的UGP2基因的表达量在MP处理下显著高于LP和HP处理。相反,在低浓度处理下,由于光合作用不足,叶片中的蔗糖很少转运到假鳞茎中,而在高浓度处理下,与碳水化合物降解和氧化有关的能量代谢受到阻碍。因此,适当的光照强度通过淀粉和蔗糖代谢介导的调节,有效地刺激了条纹草假球茎中多糖的形成。
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来源期刊
Plant Stress
Plant Stress PLANT SCIENCES-
CiteScore
5.20
自引率
8.00%
发文量
76
审稿时长
63 days
期刊介绍: The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.
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