BnaAIF1与BnaICE1的互作增强了甘蓝型油菜的低温耐受性

IF 6.8 Q1 PLANT SCIENCES
Xiaoyan Xia , Shun Li , Lei Sun, Zhonghua Wang, Xiaoyu Chen, Bo Yang, Zixuan Zhou, Xin He
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引用次数: 0

摘要

油菜(Brassica napus L.)是世界上重要的油料作物,它经常受到低温(特别是冷冻)胁迫的损害,这有可能造成重大的产量损失。然而,油菜籽应对低温胁迫的分子机制尚不清楚。本研究通过系统的生物信息学分析,在油菜中共鉴定出24个非典型bHLH转录因子AIF (ATBS1相互作用因子)基因家族成员,其中6个BnaAIF1基因均受到低温胁迫的强烈诱导,尤其是BnaAIF1- a03 /C03。过表达BnaAIF1-C03提高了冷胁迫(4°C)下油菜籽种子的萌发率和冷冻胁迫(-4°C)下幼苗的成活率,增强了光系统II的稳定性(Fv/Fm和NPQ_Lss增加),减少了氧化损伤(H2O2和MDA含量降低)。进一步分析发现,BnaAIF1-C03定位于细胞核内,与bnaaif1相互作用,激活ICE1-CBF-COR通路基因,参与油菜籽低温胁迫响应。本研究为今后耐低温油菜籽的选育提供了重要的理论依据和新的靶基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The interaction between BnaAIF1 and BnaICE1 enhances the low-temperature tolerance of Brassica napus
Rapeseed (Brassica napus L.) is an important oilseed crop in the world, it’s often damaged by low-temperature (especially freezing) stress, which has the potential to cause significant yield losses. However, little is known about the molecular mechanisms for coping with low-temperature stress in rapeseed. In this study, a total of 24 atypical bHLH transcription factor AIF (ATBS1 INTERACTING FACTOR) gene family members were identified by systematically bioinformatics analysis in rapeseed, among which all six BnaAIF1 genes were strongly induced by low temperature stress, especially BnaAIF1-A03/C03. Overexpression of BnaAIF1-C03 improved the seed germination under cold stress (4 °C) and the survival rate of seedlings under freezing stress (-4 °C) in rapeseed, with enhanced stability of light system II (Fv/Fm and NPQ_Lss increased) and reduced oxidative damage (decreased contents of H2O2 and MDA). Further analysis revealed that BnaAIF1-C03 was localized in the nucleus and interacted with BnaICE1, activating the ICE1-CBF-COR pathway genes and thus participating in the low-temperature stress response of rapeseed. Overall, this study provides an important theoretical basis and new target gene for future breeding of low-temperature tolerant rapeseed.
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来源期刊
Plant Stress
Plant Stress PLANT SCIENCES-
CiteScore
5.20
自引率
8.00%
发文量
76
审稿时长
63 days
期刊介绍: The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.
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