GABA is a key player regulating the TCA cycle and polyamine metabolism under combined heat-drought stress in tea plants

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2025-08-25 DOI:10.1016/j.stress.2025.101006

Qinqin Gao , Deng Deng , Rou Zeng , Yun Liu , Jie Jiang , Qiang Shen , Yuanchun Ma , Wanping Fang , Xujun Zhu

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引用次数: 0

Abstract

Throughout their development, plants experience a range of abiotic stresses, typically not solitary occurrences. For example, drought stress (DS) and heat stress (HS) often co-occur due to a high-temperature environment being accompanied by drought. Differing from single stress, plants have unique responses to the stress combination, with secondary metabolism holding a pivotal position in the process of plant response. Under combined stresses, plants specifically induce the accumulation of secondary metabolites to resist damage. We found that the metabolic responses of tea plants (Camellia sinensis) to DS or HS differed from those to a combination of HS and DS (HS-DS). Metabolic analysis showed that combined HS-DS led to the up-regulation and down-regulation of abundance of key metabolites in the tricarboxylic acid (TCA) cycle and polyamine metabolism pathways. Among the metabolites accumulated under combined HS-DS was γ-aminobutyric acid (GABA). Exogenous spraying of 1 mM GABA and silencing the GABA-synthesis-related gene [glutamate decarboxylase 1 (GAD1)] showed that GABA played a crucial part in the resistance of tea plants to combined HS-DS. This study reveals the function of GABA in regulating the response of tea plant to HS-DS, which provides a theoretical basis for the subsequent research on heat and drought resistance for plants.

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GABA是茶树热旱复合胁迫下TCA循环和多胺代谢的关键调控因子

在植物的整个发育过程中，它们经历了一系列的非生物胁迫，这些胁迫通常不是单独发生的。例如，由于高温环境伴随干旱，干旱胁迫（DS）和热胁迫（HS）往往同时发生。与单一胁迫不同，植物对胁迫组合的响应具有独特的特性，次生代谢在植物响应过程中起着举足轻重的作用。在联合胁迫下，植物特异性地诱导次生代谢物积累以抵抗伤害。我们发现茶树（Camellia sinensis）对DS或HS的代谢反应不同于HS和DS的组合（HS-DS）。代谢分析表明，HS-DS联合处理导致三羧酸（TCA）循环和多胺代谢途径中关键代谢产物丰度的上调和下调。在联合HS-DS下积累的代谢产物中有γ-氨基丁酸（GABA）。外源喷施1 mM GABA并沉默GABA合成相关基因[谷氨酸脱羧酶1 (GAD1)]表明，GABA在茶树抗组合HS-DS中起着至关重要的作用。本研究揭示了GABA在调节茶树对HS-DS的响应中的作用，为后续植物耐热性和抗旱性研究提供了理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.